LOCUS       NM_001204165            4220 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Rap guanine nucleotide exchange factor (GEF) 4
            (Rapgef4), transcript variant 1, mRNA.
ACCESSION   NM_001204165
VERSION     NM_001204165.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Skelin Klemen,M., Dolensek,J., Krizancic Bombek,L., Pohorec,V.,
            Gosak,M., Slak Rupnik,M. and Stozer,A.
  TITLE     The effect of forskolin and the role of Epac2A during activation,
            activity, and deactivation of beta cell networks
  JOURNAL   Front Endocrinol (Lausanne) 14, 1225486 (2023)
   PUBMED   37701894
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4220)
  AUTHORS   Guillot,B., Boileve,A., Walton,R., Harfoush,A., Conte,C.,
            Sainte-Marie,Y., Charron,S., Bernus,O., Recalde,A., Salle,L.,
            Brette,F. and Lezoualc'h,F.
  TITLE     Inhibition of EPAC1 signaling pathway alters atrial
            electrophysiology and prevents atrial fibrillation
  JOURNAL   Front Physiol 14, 1120336 (2023)
   PUBMED   36909224
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4220)
  AUTHORS   Liu,X., Vickstrom,C.R., Yu,H., Liu,S., Snarrenberg,S.T.,
            Friedman,V., Mu,L., Chen,B., Kelly,T.J., Baker,D.A. and Liu,Q.S.
  TITLE     Epac2 in midbrain dopamine neurons contributes to cocaine
            reinforcement via enhancement of dopamine release
  JOURNAL   Elife 11, e80747 (2022)
   PUBMED   35993549
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4220)
  AUTHORS   Tomilin,V.N., Pyrshev,K., Stavniichuk,A., Hassanzadeh Khayyat,N.,
            Ren,G., Zaika,O., Khedr,S., Staruschenko,A., Mei,F.C., Cheng,X. and
            Pochynyuk,O.
  TITLE     Epac1-/- and Epac2-/- mice exhibit deficient epithelial Na+ channel
            regulation and impaired urinary Na+ conservation
  JOURNAL   JCI Insight 7 (3), e145653 (2022)
   PUBMED   34914636
  REMARK    GeneRIF: Epac1-/- and Epac2-/- mice exhibit deficient epithelial
            Na+ channel regulation and impaired urinary Na+ conservation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4220)
  AUTHORS   Rehmann,H., Prakash,B., Wolf,E., Rueppel,A., de Rooij,J., Bos,J.L.
            and Wittinghofer,A.
  TITLE     Structure and regulation of the cAMP-binding domains of Epac2
  JOURNAL   Nat Struct Biol 10 (1), 26-32 (2003)
   PUBMED   12469113
REFERENCE   7  (bases 1 to 4220)
  AUTHORS   Fujimoto,K., Shibasaki,T., Yokoi,N., Kashima,Y., Matsumoto,M.,
            Sasaki,T., Tajima,N., Iwanaga,T. and Seino,S.
  TITLE     Piccolo, a Ca2+ sensor in pancreatic beta-cells. Involvement of
            cAMP-GEFII.Rim2. Piccolo complex in cAMP-dependent exocytosis
  JOURNAL   J Biol Chem 277 (52), 50497-50502 (2002)
   PUBMED   12401793
  REMARK    GeneRIF: Piccolo serves as a Ca(2+) sensor in exocytosis in
            pancreatic beta-cells and the formation of a
            cAMP-GEFII.Rim2.Piccolo complex is important in cAMP-induced
            insulin secretion
REFERENCE   8  (bases 1 to 4220)
  AUTHORS   Kashima,Y., Miki,T., Shibasaki,T., Ozaki,N., Miyazaki,M., Yano,H.
            and Seino,S.
  TITLE     Critical role of cAMP-GEFII--Rim2 complex in incretin-potentiated
            insulin secretion
  JOURNAL   J Biol Chem 276 (49), 46046-46053 (2001)
   PUBMED   11598134
REFERENCE   9  (bases 1 to 4220)
  AUTHORS   Ueno,H., Shibasaki,T., Iwanaga,T., Takahashi,K., Yokoyama,Y.,
            Liu,L.M., Yokoi,N., Ozaki,N., Matsukura,S., Yano,H. and Seino,S.
  TITLE     Characterization of the gene EPAC2: structure, chromosomal
            localization, tissue expression, and identification of the
            liver-specific isoform
  JOURNAL   Genomics 78 (1-2), 91-98 (2001)
   PUBMED   11707077
REFERENCE   10 (bases 1 to 4220)
  AUTHORS   Ozaki,N., Shibasaki,T., Kashima,Y., Miki,T., Takahashi,K., Ueno,H.,
            Sunaga,Y., Yano,H., Matsuura,Y., Iwanaga,T., Takai,Y. and Seino,S.
  TITLE     cAMP-GEFII is a direct target of cAMP in regulated exocytosis
  JOURNAL   Nat Cell Biol 2 (11), 805-811 (2000)
   PUBMED   11056535
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK220281.1 and BP767789.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK220281.1, SRR1660815.262591.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849390, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4201              AK220281.1         1-4201
            4202-4220           BP767789.1         41-59               c
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 42.9 cM"
     gene            1..4220
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /note="Rap guanine nucleotide exchange factor (GEF) 4"
                     /db_xref="GeneID:56508"
                     /db_xref="MGI:MGI:1917723"
     exon            1..137
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..3108
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cAMP-GEFII; cAMP-dependent Rap1 guanine-nucleotide
                     exchange factor; cAMP-regulated guanine nucleotide
                     exchange factor IIB; exchange factor directly activated by
                     cAMP 2; exchange protein directly activated by cAMP 2"
                     /codon_start=1
                     /product="rap guanine nucleotide exchange factor 4 isoform
                     1"
                     /protein_id="NP_001191094.1"
                     /db_xref="CCDS:CCDS57176.1"
                     /db_xref="GeneID:56508"
                     /db_xref="MGI:MGI:1917723"
                     /translation="MVAAHAAHSQSSAEWIACLDKRPLERSSEDVDIIFTRLKGVKAF
                     EKFHPNLLRQICLCGYYENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAV
                     TICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQEDFKALWEKYRQYMAGLLAP
                     PYGVMETGSNNDRIPDKENTPLIEPHVPLRPAHTITKVPSEKILRAGKILRIAILSRA
                     PHMIRDRKYHLKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQVLLEDGVLNHVDQ
                     ERHFQDKYLFYRFLDDEREDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRK
                     PPGQRTVDDLEIIYDELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGT
                     SWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDK
                     EDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRAANQGNSQPQQKYTVMSGTPEKI
                     LEHFLETIRLEPSLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYHAQPSQGTEQER
                     MDYALNNKRRVIRLVLQWAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMAAFKEQLP
                     ELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCIDHTYT
                     TIRVPVAASVKEVISAVADKLGSGEGLIIVKMNSGGEKVVLKSNDVSVFTTLTINGRL
                     FACPREQFDSLTPLPEQEGPTTGTVGTFELMSSKDLAYQMTTYDWELFNCVHELELIY
                     HTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEVCLCSQLSKRVQLLKKFIKIAAHCKE
                     YKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAA
                     KLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPD
                     AAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP"
     exon            138..280
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            281..369
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            370..516
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            517..589
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            590..609
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            610..663
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            664..770
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            771..892
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1076
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1161
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1222
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1299
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1446
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1562
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1651
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1730
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1881
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1970
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2001
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2080
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2227
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2325
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2451
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2630
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2631..2721
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2772
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2773..2860
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2861..2925
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..2980
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     exon            2981..4206
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4187..4192
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /note="hexamer: AATATA"
     polyA_site      4204
                     /gene="Rapgef4"
                     /gene_synonym="1300003D15Rik; 5730402K07Rik;
                     6330581N18Rik; EPAC 2; Epac-2; Epac2; mKIAA4040"
                     /note="major polyA site"
ORIGIN      
        1 ggccgcggga gcccgccggg aggcgcgcgg gaggtggccg cggcccgggg ccaccgcgcg
       61 ctgctgccca acatggtcgc tgcgcacgct gcacactctc agtcctcggc cgagtggatc
      121 gcctgcctgg ataaaaggcc gttggagcga tctagtgaag atgtggacat aattttcacg
      181 cggctgaaag gagttaaagc ttttgagaaa tttcacccaa acctccttcg tcagatttgt
      241 ttatgcggtt actatgagaa cctggaaaaa ggaatcacac tgtttcgcca aggggatatt
      301 ggaaccaact ggtatgctgt cctggctggg tctttggatg ttaaagtgtc tgagaccagc
      361 agtcaccagg atgcggtgac catctgcact ctgggaattg ggacagcctt tggagagtcc
      421 attctggata acacccctcg ccatgcaacc atcgttacca gggagagcag cgaacttctc
      481 cgcattgagc aggaggactt caaggcacta tgggagaaat accgacagta tatggccgga
      541 cttctggctc ctccctatgg tgttatggaa acgggctcta acaatgacag gattcctgac
      601 aaggagaata cacctctcat tgaaccccac gttcctctcc gtcctgctca caccattacc
      661 aaggtccctt cagagaagat cctcagagct ggaaaaattt tacgaattgc cattctctct
      721 cgagctcccc acatgataag agacagaaag taccacctaa agacatacag acaatgctgt
      781 gttgggactg agctggtaga ctggatgata cagcagacat cctgtgttca ctcgcggact
      841 caagctgttg gcatgtggca agtcttgctg gaagatggtg tcctcaacca tgtggaccag
      901 gagcgccatt tccaagacaa atatttattt tatcgatttc tggatgacga gcgtgaggat
      961 gcccctttgc ctactgagga agagaagaag gagtgtgatg aagaacttca ggacaccatg
     1021 ctgctgctct cacagatggg ccctgacgcc cacatgagaa tgatcctgcg aaaaccacct
     1081 ggccagagga ctgtggatga cctagagatt atctacgacg agctccttca tattaaagcc
     1141 ttatcccatc tctctaccac agtgaaacgg gagttagcag gtgttctcat ttttgagtct
     1201 cacgccaaag gaggaactgt gttgtttaac cagggggaag aaggtacctc ctggtacatc
     1261 attctgaaag gatccgtgaa tgtagtcatt tatggcaagg gtgtggtctg caccctgcac
     1321 gaaggagatg actttggcaa gttagctcta gtgaacgatg ctccaagagc tgcctccatt
     1381 gttcttcggg aagataattg tcacttccta agagtcgaca aggaagactt caatcggatt
     1441 ctgagggacg ttgaggcgaa tacagtcaga cttaaagaac atgaccaaga tgtcttggta
     1501 ctggagaagg tcccagcagg gaacagagct gctaatcaag gaaactcaca gcctcagcaa
     1561 aagtatactg tgatgtcagg aacacctgaa aagattttag agcattttct agaaacaata
     1621 cgccttgagc catcgttgaa tgaagcaaca gattcggttt taaatgactt tgttatgatg
     1681 cactgtgttt ttatgccaaa tacccagctt tgccctgccc ttgtggccca ttaccacgca
     1741 cagccttctc aaggtaccga gcaggagaga atggattatg ccctcaacaa caagaggcgg
     1801 gtcatccgct tggtcctgca gtgggcggcc atgtatggcg atctcctcca agaagatgat
     1861 gtggccatgg ccttcctgga ggagttctat gtgtctgtat cagatgacgc acggatgatg
     1921 gctgccttca aggagcagct gccagagctg gagaagattg tcaagcaaat ctcagaagac
     1981 gcaaaagctc cacagaagaa gcacaaggtg cttttgcaac agttcaacac aggtgacgag
     2041 agggcccaga agcgtcagcc tattcgtggc tctgatgagg ttttgttcaa ggtctactgc
     2101 atcgaccaca cctatactac cattcgtgtg ccggtagctg cctcggtgaa ggaagtcatc
     2161 agtgcagtag ctgacaaact gggctcaggg gaaggcctga tcatcgtcaa gatgaactct
     2221 ggaggagaaa aggtggtgct gaaatctaat gatgtttcag tatttacgac gctcaccatt
     2281 aatggacgcc tgtttgcctg cccgagagag caattcgact cactgactcc cttgccggaa
     2341 caggaaggcc cgaccactgg gacagtggga acatttgagc tgatgagctc gaaagacctg
     2401 gcgtaccaga tgacaaccta cgattgggaa ctcttcaact gtgtgcatga gctggagcta
     2461 atctaccaca catttggaag gcataatttt aaaaagacca cggcaaactt ggatttgttc
     2521 ctgaggaggt ttaatgaaat tcagttttgg gttgtcactg aggtctgcct ttgttcccag
     2581 ctcagcaaac gtgttcagct tttgaaaaaa tttatcaaga tagcggctca ctgcaaggag
     2641 tacaaaaatc taaattcctt tttcgccatc gtcatgggac tcagcaacgt ggccgtgagc
     2701 cgcttggcac taacgtggga gaaactgccg agcaagttta agaagttcta tgcggagttt
     2761 gagagcttga tggatccttc cagaaaccac agggcataca ggctgacagc agccaagctg
     2821 gagccccctc tcatcccttt catgcccttg cttattaaag atatgacatt tactcatgag
     2881 gggaacaaga cgttcattga caatctagta aactttgaaa aaatgcgcat gattgcaaac
     2941 actgccagaa cagtacggta ctacaggagc cagcccttca atccggatgc cgctcaagct
     3001 aataagaacc atcaggatgt ccggagttat gtacggcaat taaatgtgat tgacaaccag
     3061 agaactttat cacagatgtc acacagatta gagcctcgaa ggccatagac atctgaagtg
     3121 cccagagtga tgttttgtct ccagtccaca atctttcaaa agatgctgtg tatgctacta
     3181 ctgactgtgt tgctactaga gaattctcca gaatgagcaa gagacacatc ctgagagccc
     3241 ctcggggcta catcctgctt tccaaccaca caggagaagc gatctgactt gtctaatgct
     3301 gccatgctgt agcttaggaa tcatcagtta tagtcaccct ccttcattgt tggcacgcca
     3361 gcaccaggct cacgtgaaga aataaccttg gcgggaggct acatcagggc cctgacatcc
     3421 cttcccaggc tgtttgcagc atgagactgt gccatacgta gataggtttc actcggatgc
     3481 gccggcgatg atataattga atgaattgtt tacttagcac cttatttggg gtctggggtc
     3541 tggggagggt attgaggata ccgtaaaagt ccaaattata catcgtgttc ctccatgttc
     3601 ataatctaac ctctgaagga atggaacctc accacaacac tatgaaacat tatggctgtt
     3661 tcgtctgtaa tttcggaatg atctatagaa taatatgttt acattgtaac ttttaaaaac
     3721 taacaaatca ggattgcata cataagaatg ccactaagaa acaccaaggt tcttaatacc
     3781 gccagcgtta agatagaaag taacatttca gaagagcaca atatgcacaa aacatttttt
     3841 caaaatttaa atattttcct gggcattaaa aacgtttcca ctacaaattt attgttactg
     3901 atgaaaaaat gcatattttc tggagttaaa tgttattaca aaaatcttaa ttttcagcaa
     3961 ttgttttgca ctttcagata gattgtaaat aggttatgca gtcaatgata tagagttatt
     4021 tatttgctac ataatagaca ttgtgccaaa taattccttt ttatttattt tattcagtat
     4081 gaaattttgg agtacatttt ttctgttttc ttaattagac cacatttaat gtataggaat
     4141 tgtatgtaca tatctcttct gtaaataaca gccagtatct tcattaaata tacttgacaa
     4201 gaaataaaaa aaaaaaaaaa
//
