LOCUS       NM_001310536            7347 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus Rap guanine nucleotide exchange factor (GEF) 2
            (Rapgef2), transcript variant 2, mRNA.
ACCESSION   NM_001310536 XM_006502254
VERSION     NM_001310536.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   Jiang,S.Z., Shahoha,M., Zhang,H.Y., Brancaleone,W., Elkahloun,A.,
            Tejeda,H.A., Ashery,U. and Eiden,L.E.
  TITLE     The guanine nucleotide exchange factor RapGEF2 is required for
            ERK-dependent immediate-early gene (Egr1) activation during fear
            memory formation
  JOURNAL   Cell Mol Life Sci 81 (1), 48 (2024)
   PUBMED   38236296
  REMARK    GeneRIF: The guanine nucleotide exchange factor RapGEF2 is required
            for ERK-dependent immediate-early gene (Egr1) activation during
            fear memory formation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7347)
  AUTHORS   Jang,Y.N., Jang,H., Kim,G.H., Noh,J.E., Chang,K.A. and Lee,K.J.
  TITLE     RAPGEF2 mediates oligomeric Abeta-induced synaptic loss and
            cognitive dysfunction in the 3xTg-AD mouse model of Alzheimer's
            disease
  JOURNAL   Neuropathol Appl Neurobiol 47 (5), 625-639 (2021)
   PUBMED   33345400
  REMARK    GeneRIF: RAPGEF2 mediates oligomeric Abeta-induced synaptic loss
            and cognitive dysfunction in the 3xTg-AD mouse model of Alzheimer's
            disease.
REFERENCE   4  (bases 1 to 7347)
  AUTHORS   Jiang,S.Z., Sweat,S., Dahlke,S.P., Loane,K., Drossel,G., Xu,W.,
            Tejeda,H.A., Gerfen,C.R. and Eiden,L.E.
  TITLE     Cocaine-Dependent Acquisition of Locomotor Sensitization and
            Conditioned Place Preference Requires D1 Dopaminergic Signaling
            through a Cyclic AMP, NCS-Rapgef2, ERK, and Egr-1/Zif268 Pathway
  JOURNAL   J Neurosci 41 (4), 711-725 (2021)
   PUBMED   33268547
REFERENCE   5  (bases 1 to 7347)
  AUTHORS   Arimura,N., Okada,M., Taya,S., Dewa,K.I., Tsuzuki,A., Uetake,H.,
            Miyashita,S., Hashizume,K., Shimaoka,K., Egusa,S., Nishioka,T.,
            Yanagawa,Y., Yamakawa,K., Inoue,Y.U., Inoue,T., Kaibuchi,K. and
            Hoshino,M.
  TITLE     DSCAM regulates delamination of neurons in the developing midbrain
  JOURNAL   Sci Adv 6 (36) (2020)
   PUBMED   32917586
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7347)
  AUTHORS   Wei,P., Satoh,T., Edamatsu,H., Aiba,A., Setsu,T., Terashima,T.,
            Kitazawa,S., Nakao,K., Yoshikawa,Y., Tamada,M. and Kataoka,T.
  TITLE     Defective vascular morphogenesis and mid-gestation embryonic death
            in mice lacking RA-GEF-1
  JOURNAL   Biochem Biophys Res Commun 363 (1), 106-112 (2007)
   PUBMED   17826737
  REMARK    GeneRIF: These results suggest that deficient Rap1 signaling may
            lead to defective vascular morphogenesis in the yolk sac and
            embryos proper.
REFERENCE   7  (bases 1 to 7347)
  AUTHORS   Amsen,E.M., Pham,N., Pak,Y. and Rotin,D.
  TITLE     The guanine nucleotide exchange factor CNrasGEF regulates
            melanogenesis and cell survival in melanoma cells
  JOURNAL   J Biol Chem 281 (1), 121-128 (2006)
   PUBMED   16272156
  REMARK    GeneRIF: CNrasGEF regulates melanogenesis but that it also has a
            distinct role in regulating cell proliferation/apoptosis
REFERENCE   8  (bases 1 to 7347)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   9  (bases 1 to 7347)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 7347)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Hara,Y., Nagase,T.,
            Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: I. The complete nucleotide sequences of 100 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 9 (5), 179-188 (2002)
   PUBMED   12465718
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124358.4 and AC164560.3.
            
            On or before Feb 12, 2019 this sequence version replaced
            XM_006502254.3, NM_001310536.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and uses a downstream in-frame
            start site compared to variant 1. The encoded isoform (2) has a
            shorter N-terminus than isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147368.1, SRR10223970.5246.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-891               AC124358.4         14838-15728
            892-1023            AC124358.4         48282-48413
            1024-1201           AC124358.4         56444-56621
            1202-1338           AC164560.3         1863-1999
            1339-1497           AC164560.3         2715-2873
            1498-1650           AC164560.3         6128-6280
            1651-1815           AC164560.3         6644-6808
            1816-1923           AC164560.3         7991-8098
            1924-2127           AC164560.3         8190-8393
            2128-2236           AC164560.3         8488-8596
            2237-2483           AC164560.3         9035-9281
            2484-2641           AC164560.3         12291-12448
            2642-2882           AC164560.3         13008-13248
            2883-3266           AC164560.3         14965-15348
            3267-3478           AC164560.3         17473-17684
            3479-3602           AC164560.3         17771-17894
            3603-3626           AC164560.3         18446-18469
            3627-3850           AC164560.3         19808-20031
            3851-4057           AC164560.3         21881-22087
            4058-4210           AC164560.3         26633-26785
            4211-4436           AC164560.3         30878-31103
            4437-4996           AC164560.3         31645-32204
            4997-5296           AC164560.3         34221-34520
            5297-7347           AC164560.3         36532-38582
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 34.91 cM"
     gene            1..7347
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /note="Rap guanine nucleotide exchange factor (GEF) 2"
                     /db_xref="GeneID:76089"
                     /db_xref="MGI:MGI:2659071"
     exon            1..891
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             832..5322
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /note="isoform 2 is encoded by transcript variant 2; rap
                     guanine nucleotide exchange factor 2; neural RAP guanine
                     nucleotide exchange protein; PDZ domain-containing guanine
                     nucleotide exchange factor 1; cyclic nucleotide ras GEF;
                     ras/Rap1-associating GEF-1"
                     /codon_start=1
                     /product="rap guanine nucleotide exchange factor 2 isoform
                     2"
                     /protein_id="NP_001297465.2"
                     /db_xref="CCDS:CCDS89637.1"
                     /db_xref="GeneID:76089"
                     /db_xref="MGI:MGI:2659071"
                     /translation="MKPLAAPANHGVLGQQEKQSLPADFTKLHLTDSLHPQVTHVSSS
                     HSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLM
                     SRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAG
                     TIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKV
                     DDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTS
                     ERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVL
                     LWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPS
                     REAPLPFILLGGSEKGFGIFVDSVDSCSKATEAGLKRGDQILEVNGQNFENIQLSKAM
                     EILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVD
                     VEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGL
                     RQTKHIPAALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMI
                     SKDTTAKEVVIQAIREFAVTATPEQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLS
                     GRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTE
                     YIDDLFKLKSKTSCANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHC
                     RECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNV
                     LSGQNLQPPVIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVN
                     MDPALMFRTRKKKWRSLGSLSQGSANATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQ
                     MARKVKQYLSNLELEMDEESLQTLSLQCEPATSTLPKNPGDKKPVKSETSPVAPRAGP
                     QQKVQPQQPLAQPQPPHKVSQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILS
                     LSEEGSLERHRKQAEDTISNASSQLSSPPTSPQSSPRKGYALALSGTVDNFSDSGHSE
                     ISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVESNLGVGRMERRTLMEPDQYSLGSY
                     APVSESRGLYAAATVISSPSTEELSHDQGDRASLDAADSGRGSWTSCSSGSHDNIQTI
                     QHQRSWETLPFGHTHFDYSGDAASIWASGGHMDQMMFSDHSTKYNRQNQSRESLEQAQ
                     SRASWASSTGYWGEDSEGDTGTIKRRGGKDVSAEAESSSMVPVTTEEAKPVPMPAHIA
                     VTPSTTKGLIARKEGRYREPPPTPPGYVGIPIADFPEGPCHPARKPPDYNVALQRSRM
                     VARPTEAPAPGQTPPAAAASRPGSKPQWHKPSDADPRLAPFQPQGFAGAEEDEDEQVS
                     AV"
     exon            892..1023
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1201
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1338
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1497
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1650
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1815
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1923
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2127
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2236
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2483
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2484..2641
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2642..2882
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..3266
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3478
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3479..3602
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3603..3626
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3627..3850
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3851..4057
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4058..4210
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4211..4436
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4437..4996
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4997..5296
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5297..7347
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7314..7319
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /note="hexamer: AATAAA"
     regulatory      7325..7330
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /note="hexamer: CATAAA"
     polyA_site      7330
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
                     /note="major polyA site"
     polyA_site      7342
                     /gene="Rapgef2"
                     /gene_synonym="5830453M24Rik; CNRasGEF; mKIAA0313;
                     nRapGEP; Pdzgef1; RA-GEF-1"
ORIGIN      
        1 tggtgtattg cattcagact catgattctt gagattgtgt gtgtgtgtgt gtgtgtgtgt
       61 gtgtgtgtgc atgttccatg tttctgtcag tgactccggc tagcaggcat gcttgtgttg
      121 agtgtgaggg acagacatca ttaaattaaa tgtccatcag tgtcatactg ctgagattaa
      181 ttgtgtaaac ttggttagtg gagtgggaca gagatcactg gtcaccactg tcagcatcat
      241 tagcagctag ggaggaggtc ctgattcaca cgctctgccc tgattttttt ttctcttcct
      301 ctcttttttt tccttggttg gaggggtctg tatgtgtgat tgcaaatgaa acaagagtgt
      361 taacaatgct tggctgaatg ctagagacta gcttttcacc accctggcta atataattct
      421 gtattaagcc ttttggaggg gagtctgtgt gcagtcagcc attttagctt ttttttcccc
      481 ccttttcttt tgcctcttgt tcttcagcct cagaaatgat ctcgttctat cagaagcctg
      541 aaggctgggt gaggttccct cagccttgtc agagcctccg agggttagag tttcagtgtg
      601 ataaaaatgg agccgagccc tgtcgttcag attccttttc tgtctacaag tgtgaagggt
      661 ttttaggctc accagtagtg acactttaag ctgcagcctg gttttccctc ctccctcttt
      721 ttttttttcc tcccctcttt ttcttttaat ttcatgctgt agagggtgac ttgccgtcgt
      781 gagaggttgg tacatgatgt gtaaattcag ttcagcatat gtttcttcat tatgaaacca
      841 ctggccgcgc ctgctaacca tggagttctg ggccagcagg agaagcagtc tctccctgca
      901 gacttcacaa aactgcacct cactgacagc ctgcacccac aggtgaccca cgtgtcctcc
      961 agccattcag gatgtagcat caccagcgac tctgggagca gcagtctttc tgacatttac
     1021 caggccacgg agagcgaggc cggtgacatg gacctgagtg gactgccaga gacggcggtt
     1081 gactctgagg atgacgatga tgaggaagac atcgagagag catcagaccc tctgatgagc
     1141 agggacatcg tgagggactg cctggagaag gacccgatag acaggactga cgatgacatc
     1201 gaacaactac tggaatttat gcaccagctg ccggcctttg ccaatatgac gatgtcagta
     1261 agacgggagc tctgtgccgt gatggtgttt gcagtggtgg aaagagctgg aaccattgtg
     1321 ttaaacgacg gagaagagct ggattcctgg tcagtgattc tcaatggttc cgtggaggtg
     1381 acatatccgg atgggaaagc agaaattctg tgcatgggaa acagctttgg tgtttcgccc
     1441 accatggaca aagaatacat gaaaggagtc atgaggacta aggtggatga ctgccagttt
     1501 gtctgcattg cccagcaaga ttactgccgt attttgaacc aagtagaaaa gaacatgcaa
     1561 aaagttgagg aggaaggaga gattgtaatg gtgaaggagc accgagaact tgatcggact
     1621 ggaacaagga agggccacat cgtcatcaag ggcacctcag agaggctgac catgcatttg
     1681 gtggaagagc attctgtagt ggatccaaca ttcatagaag acttcctgtt gacataccgg
     1741 actttccttt ccagcccgat ggaagtgggc aagaagttat tggagtggtt caatgacccg
     1801 agcctcaggg ataaggtgac gcgggtagta ttactgtggg tgaataatca cttcaatgac
     1861 tttgaagggg atcctgcaat gactcgattt ctggaagaat ttgaaaataa tctggaaaga
     1921 gagaaaatgg gtggacatct aaggctgttg aacattgcat gtgcagctaa agccaaacga
     1981 agactgatga cgttaacaaa gccttcccgg gaagcccctc tgcccttcat cttactggga
     2041 ggctctgaaa agggctttgg catcttcgtg gacagcgttg actcgtgtag caaagccact
     2101 gaagcaggct tgaagcgcgg ggatcagata ttagaagtga atggtcaaaa ttttgaaaat
     2161 atccaacttt caaaggcaat ggaaattctc agaaataaca cacacctgtc catcactgtg
     2221 aaaaccaact tatttgtatt taaagaactt ctgacgagat tgtctgaaga gaaaagaaac
     2281 ggtgcccctc acctccctaa aatcggggac atcaagaagg ccagccgcta ctccataccg
     2341 gatctcgccg tagacgtaga acaggtgata ggcctcgaga aggtcaacaa gaaaagcaaa
     2401 gccaacaccg tgggaggaag aaacaagctg aagaaaatac tcgacaagac ccggatcagc
     2461 atcttacctc agaaaccata caatgatatt gggattggtc agtctcaaga tgatagcatc
     2521 gtaggactga ggcagaccaa gcacatccca gctgccctgc ctgtcagtgg aacgctgtcc
     2581 tccagtaacc ctgacttgct gcagtcacac caccggattc tggacttcag cactactcct
     2641 gacttgccag atcaggtact gagagttttc aaggctgacc aacaaagcag gtacatcatg
     2701 atcagcaagg acaccaccgc aaaggaagtg gtcatccagg ccatcagaga gtttgctgtc
     2761 actgccaccc cagagcagta ttccctctgt gaggtctcag tcacaccaga gggagttatc
     2821 aaacaacgcc gactcccaga tcagctttcc aagcttgccg acaggataca actgagtgga
     2881 aggtattatc tgaaaaacaa catggaaaca gaaacgcttt gctcagatga agatgcccag
     2941 gagctgctgc gagagagcca aatctccctc cttcagctca gcaccgtgga ggttgccacg
     3001 cagctctcca tgaggaactt tgaactcttc cgcaacattg agcctactga atacatagat
     3061 gatttattta aactcaaatc aaaaaccagc tgtgccaacc tgaagaaatt tgaggaagtc
     3121 attaaccagg aaacattttg ggtggcttct gaaatcctgc gggaaacaaa ccagctgaag
     3181 aggatgaaga tcattaagca tttcatcaag attgccttgc actgcaggga atgcaagaat
     3241 tttaactcca tgtttgcaat catcagtggc ctaaacctgg caccggtggc aagactacga
     3301 accacctggg aaaagcttcc caataaatat gaaaagctgt tccaggacct gcaagacctg
     3361 tttgatcctt ccagaaacat ggcaaagtac cgcaatgtcc tgagtggcca gaacttacag
     3421 cctcctgtca ttcccctgtt ccctgtcatc aagaaggacc tcacgtttct ccatgaggga
     3481 aatgactcaa aagtagacgg actggtcaac tttgagaagc tgaggatgat tgcaaaagaa
     3541 atccgtcacg ttggccgaat ggcatcagtc aacatggacc ctgcccttat gttcaggact
     3601 cggaagaaga agtggcggag tctggggtcc ctgagtcagg gcagtgcaaa cgccacagtg
     3661 ctggatgttg ctcagacagg cgggcataaa aagcgggtac gccgtagttc cttcctcaat
     3721 gccaaaaagc tgtatgaaga tgcccagatg gctcggaaag tgaagcaata cctgtccaac
     3781 ctggagctgg agatggatga ggagagtctt cagacactgt cactgcagtg tgaaccagca
     3841 accagcacat tgcccaagaa tcctggagac aaaaagcctg tcaagtctga gacctcccca
     3901 gtggctccac gtgcagggcc ccagcagaaa gtacagcctc agcagccgct ggcacagcca
     3961 caacctccac ataaagtcag ccagggactg caggtgcccg ctgtgtccct ctatccttca
     4021 cgaaagaagg tgccggtgaa ggatctgcct ccttttggca taaactctcc acaagctttg
     4081 aagaaaatcc tgtccttgtc tgaagaaggg agtttggagc gtcacaggaa gcaagcagaa
     4141 gatacaatat caaatgcgtc ctctcagctc tcatcgcccc ccacctcacc tcagagctct
     4201 ccaaggaaag gttatgcact ggctctcagt ggcaccgtgg ataatttctc agattcaggt
     4261 cacagtgaga tttcttcacg atccagtatt gtcagcaatt cttcttttga ctcggtgcca
     4321 gtctctctgc atgatgaacg gcgccagagg cattctgtca gcattgtgga gagcaaccta
     4381 ggtgtgggca ggatggagcg gcggaccctg atggagcctg atcaatacag cttagggtcg
     4441 tatgccccag tgtctgaaag ccgaggcctg tatgccgcag cgacggtcat ctcttctcct
     4501 agcacagagg agctttccca tgaccaaggg gaccgtgctt ccctcgatgc tgctgacagc
     4561 ggtcgtggga gctggacatc atgctcaagt ggttcccatg acaacataca gaccattcag
     4621 caccagagaa gctgggaaac actgccattt gggcacactc actttgacta ttcaggggat
     4681 gctgcaagta tatgggcctc cggtggccat atggaccaaa tgatgttttc tgatcatagc
     4741 acaaaatata acagacagaa tcaaagtaga gagagtcttg aacaagccca atcccgggcc
     4801 agctgggcgt cttccacggg ctactgggga gaagactcag aaggcgacac aggcactatc
     4861 aagcgaaggg gtgggaagga tgtctccgct gaggcagaga gcagcagcat ggtgcccgtg
     4921 actacagagg aagccaaacc tgtccctatg cctgcccaca tagctgtgac gccgagcact
     4981 accaagggac tcatcgcacg gaaggaaggc aggtaccggg agccgcctcc cacacctcca
     5041 ggctacgtgg gcatccccat tgccgatttc ccagaagggc cttgccaccc ggccaggaag
     5101 cccccggatt acaacgtggc cctgcagcgg tcccgcatgg tggcacggcc cactgaggcc
     5161 ccggcaccgg gccagacgcc gcctgcagcc gcagccagcc ggccgggcag caagccacag
     5221 tggcacaagc ccagcgacgc agacccacgc ctcgcgccct tccagccgca gggcttcgca
     5281 ggagcggagg aggacgaaga tgaacaagtg tctgctgttt gaggcccagg ctccttgatc
     5341 cacagtgagc cacccaaagg agagcacaag aagacgtccc aagccttgga gccttggcac
     5401 gcacatctga ggatggtgga ccagtttgcc tccttccctg ccttaaagca gcatggggct
     5461 tcttctcccc ttcttccttt cccctttgca tgtgaaatac tgtgaagaaa ttgccctggc
     5521 actttgcaga cttgttgctt gaaatgcaca gcccagcagc ccctgagctg ctgcctgcca
     5581 cgtcacgcag tatcattcca aattccaaga tcgtcacaag gcgattccct ctggcggccc
     5641 ttctccatgc ctggaaggag attccaatct tcctcttaga tttcaatcca gtcctagccc
     5701 tcgatctcat ttggaagatg agaaaagcta gcctttgaac tacccggggt ctcgaaccca
     5761 ccaaggaaga caaagactga tatgaaatcc tttgagtaca gtgcttgtcc acttgtttac
     5821 aatatccttt aaaaaaaaat aagtttaaag attgtgttca gagagtaaat gttatatcca
     5881 ttttatgatt acagtattat tttgaacctt aagtaggatt gccagcctgg gtttctgaaa
     5941 aaccaaatat gccggacagg gtttggccgc accaagggaa gacctgggca gtgaccctgt
     6001 cttcccatgt ccttctggcc ttgcccgtga agtgccctat cccagaagca tcaaacgtta
     6061 gccaattact taaataccaa gactcctcac ctgccccttc cccagtgggt gaggctctaa
     6121 tgtaaaacag tttgcacatg gccaggggag ggaactagga cttgtgtgtc ctgtctgagc
     6181 cttatgaggc aggatggtgt tatttaagag tgtgtcctgc tcaattacaa tggatggcaa
     6241 cccctgtttt taagttatgt ttgtttgttt ttttgttttt gggttttttt gtttttttgt
     6301 tttttttttt agttttgtta atttagggtt ttagagcttt ttgtttttat tttttaaaag
     6361 aaacatttat aactggatag cattgcagtg aggcagcttg gggtgttgga gcgaatgcca
     6421 gctgtttgta ctgctctttc aagacagcct ccccttgttg gattggcacc agagagtatg
     6481 tctttaaaca tgattaaagg tcacaaacat ggttaaatac gccagccttt gcaaaatggt
     6541 tagtcaccaa aggctgacct gtaagtggct ttggggacgc tgcatatgga gaaggccaag
     6601 tgtagcgccc gtctgctcat agctgctgct agccctagaa tgactgaaaa tgaccccgcc
     6661 ggtctattct tgtgttgttt ttgcacagac tccggaaaag tgaaggctgc caatccgagt
     6721 agtactcaga tgtgaggggc tgctgatctt ggatttttcc attaaattca gctgatcata
     6781 ttgatcagta gataaacgta aatagcttca aattttaaag gtcgaattgc agtgtttttt
     6841 cactgtgtca aacaatgtca gtgctttatt taataattct cttctatgtc atggcgtttg
     6901 tctacttgtg tatcacattg tcacttacgc atttgtaatt ttccatgtaa tatgcattat
     6961 tgccagtttt actctatagg ctatggacct catgtgcata tagaaagaca aacctctagc
     7021 tctaccacaa attgcacaga tgttatctaa gcattaagta attgtagaac ataggactgc
     7081 taatctcagt tcgctctgtg atgtcaagtg cagaatgtac aattaactgg tgatttcctc
     7141 atgcttttga taccacttgt acctgtatgt cttttagaaa gacattggtg gagtctgtat
     7201 cccttttgta tttttaatac aataattgta catattggtt atatttttgt tgaagatggt
     7261 agaaatgtac tatgtttatg cttctacatc cagtttgtac aagctggaga ataaataaat
     7321 ataacataaa gccttgtcta aactttt
//
