LOCUS       NM_029519               4174 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus RAS related protein 2a (Rap2a), mRNA.
ACCESSION   NM_029519
VERSION     NM_029519.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Qi,H., Zhang,Y., Xu,L., Zheng,X., Li,Y., Wei,Q., Li,Y., Zhao,Z. and
            Fang,J.
  TITLE     Loss of RAP2A Aggravates Cartilage Degradation in TMJOA via YAP
            Signaling
  JOURNAL   J Dent Res 102 (3), 302-312 (2023)
   PUBMED   36366779
  REMARK    GeneRIF: Loss of RAP2A Aggravates Cartilage Degradation in TMJOA
            via YAP Signaling.
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Carvalho,B.C., Oliveira,L.C., Rocha,C.D., Fernandes,H.B.,
            Oliveira,I.M., Leao,F.B., Valverde,T.M., Rego,I.M.G., Ghosh,S. and
            Silva,A.M.
  TITLE     Both knock-down and overexpression of Rap2a small GTPase in
            macrophages result in impairment of NF-kappaB activity and
            inflammatory gene expression
  JOURNAL   Mol Immunol 109, 27-37 (2019)
   PUBMED   30851634
  REMARK    GeneRIF: These results implicate Rap2a in TLR-mediated responses by
            contributing to balanced NF-kappaB activity status in macrophages.
REFERENCE   3  (bases 1 to 4174)
  AUTHORS   Zhang,L., Zhang,P., Wang,G., Zhang,H., Zhang,Y., Yu,Y., Zhang,M.,
            Xiao,J., Crespo,P., Hell,J.W., Lin,L., Huganir,R.L. and Zhu,J.J.
  TITLE     Ras and Rap Signal Bidirectional Synaptic Plasticity via Distinct
            Subcellular Microdomains
  JOURNAL   Neuron 98 (4), 783-800 (2018)
   PUBMED   29706584
REFERENCE   4  (bases 1 to 4174)
  AUTHORS   Xue,Q., Yu,C., Wang,Y., Liu,L., Zhang,K., Fang,C., Liu,F., Bian,G.,
            Song,B., Yang,A., Ju,G. and Wang,J.
  TITLE     miR-9 and miR-124 synergistically affect regulation of dendritic
            branching via the AKT/GSK3beta pathway by targeting Rap2a
  JOURNAL   Sci Rep 6, 26781 (2016)
   PUBMED   27221778
  REMARK    GeneRIF: The small GTP-binding protein Rap2a is a common target of
            both miR-9 and miR-124. miR-9 and miR-124 together, but neither
            miRNA alone, strongly suppressed Rap2a, thereby promoting neuronal
            differentiation of neural stem cells and dendritic branching of
            differentiated neurons. Rap2a also diminished the dendritic
            complexity of mature neurons by decreasing the levels of pAKT and
            pGSK3beta.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4174)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   6  (bases 1 to 4174)
  AUTHORS   McLeod,S.J., Shum,A.J., Lee,R.L., Takei,F. and Gold,M.R.
  TITLE     The Rap GTPases regulate integrin-mediated adhesion, cell
            spreading, actin polymerization, and Pyk2 tyrosine phosphorylation
            in B lymphocytes
  JOURNAL   J Biol Chem 279 (13), 12009-12019 (2004)
   PUBMED   14701796
  REMARK    GeneRIF: Rap2 has a role in regulation of integrin-mediated
            adhesion, cell spreading, actin polymerization, and Pyk2 tyrosine
            phosphorylation in B lymphocytes
REFERENCE   7  (bases 1 to 4174)
  AUTHORS   Christian,S.L., Lee,R.L., McLeod,S.J., Burgess,A.E., Li,A.H.,
            Dang-Lawson,M., Lin,K.B. and Gold,M.R.
  TITLE     Activation of the Rap GTPases in B lymphocytes modulates B cell
            antigen receptor-induced activation of Akt but has no effect on
            MAPK activation
  JOURNAL   J Biol Chem 278 (43), 41756-41767 (2003)
   PUBMED   12904304
  REMARK    GeneRIF: BCR-induced phosphorylation of Akt on critical activating
            sites was increased 2- to 3-fold when Rap1 and Rap2 activation was
            blocked
REFERENCE   8  (bases 1 to 4174)
  AUTHORS   Traver,S., Bidot,C., Spassky,N., Baltauss,T., De Tand,M.F.,
            Thomas,J.L., Zalc,B., Janoueix-Lerosey,I. and Gunzburg,J.D.
  TITLE     RGS14 is a novel Rap effector that preferentially regulates the
            GTPase activity of galphao
  JOURNAL   Biochem J 350 Pt 1 (Pt 1), 19-29 (2000)
   PUBMED   10926822
REFERENCE   9  (bases 1 to 4174)
  AUTHORS   Smallwood,P.M., Munoz-Sanjuan,I., Tong,P., Macke,J.P., Hendry,S.H.,
            Gilbert,D.J., Copeland,N.G., Jenkins,N.A. and Nathans,J.
  TITLE     Fibroblast growth factor (FGF) homologous factors: new members of
            the FGF family implicated in nervous system development
  JOURNAL   Proc Natl Acad Sci U S A 93 (18), 9850-9857 (1996)
   PUBMED   8790420
REFERENCE   10 (bases 1 to 4174)
  AUTHORS   Lee,C.G., Jenkins,N.A., Gilbert,D.J., Copeland,N.G. and
            O'Brien,W.E.
  TITLE     Cloning and analysis of gene regulation of a novel LPS-inducible
            cDNA
  JOURNAL   Immunogenetics 41 (5), 263-270 (1995)
   PUBMED   7721348
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CT009559.20
            and AC164304.2.
            
            On Nov 17, 2006 this sequence version replaced NM_029519.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053003.1, AK075712.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-580               CT009559.20        70885-71464
            581-4174            AC164304.2         19754-23347
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 64.72 cM"
     gene            1..4174
                     /gene="Rap2a"
                     /gene_synonym="5830461H18Rik"
                     /note="RAS related protein 2a"
                     /db_xref="GeneID:76108"
                     /db_xref="MGI:MGI:97855"
     exon            1..580
                     /gene="Rap2a"
                     /gene_synonym="5830461H18Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..818
                     /gene="Rap2a"
                     /gene_synonym="5830461H18Rik"
                     /EC_number="3.6.5.2"
                     /codon_start=1
                     /product="ras-related protein Rap-2a"
                     /protein_id="NP_083795.2"
                     /db_xref="CCDS:CCDS27341.1"
                     /db_xref="GeneID:76108"
                     /db_xref="MGI:MGI:97855"
                     /translation="MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEI
                     EVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIR
                     VKRYEKVPVILVGNKVDLESEREVSSNEGRALAEEWGCPFMETSAKSKTMVDELFAEI
                     VRQMNYAAQPDKDDPCCSACNIQ"
     exon            581..4174
                     /gene="Rap2a"
                     /gene_synonym="5830461H18Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4154..4159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rap2a"
                     /gene_synonym="5830461H18Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4174
                     /gene="Rap2a"
                     /gene_synonym="5830461H18Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcgggcccgc ggttcgggcg gctccggctc gaggtcgagc tctgttgctg ctctcgccct
       61 cggggctgca tcgtcctccc gcttggcccg ctcacagggc cgcgcacgac tcgttcagct
      121 gcagccccgc tgccggcgcc cggggcgctg ccgcggccgt gagccgacga gtccgtaagg
      181 gcggcggggc cgcggggtcg gggcgcggcg cgcgggcgca ggtctatgtc gcgggcggcg
      241 gcggcggcgg cggccgcgca gggacgatgc gcgagtacaa agtggtggtg ctgggctcgg
      301 gcggggtagg caaatccgcc ctgaccgtgc agttcgtgac cggcaccttc attgagaaat
      361 acgaccccac catcgaggac ttctaccgca aggagatcga ggtggactcc tcgccttcgg
      421 tgctggagat cctggacacc gcgggcaccg agcagttcgc gtccatgcgg gacctgtaca
      481 tcaagaacgg ccagggcttc atcctggtct acagcttggt caaccagcag agcttccaag
      541 acatcaagcc catgcgggac cagatcatcc gcgtgaagcg gtatgagaag gttccagtca
      601 tcctggtcgg gaacaaagtg gacctggaga gtgagagaga agtgtcctcc aacgaaggcc
      661 gcgccttggc cgaggagtgg ggctgcccct ttatggagac ttctgctaag agcaaaacaa
      721 tggtggatga gctctttgca gaaattgtgc ggcagatgaa ctatgcagct cagcctgaca
      781 aagatgaccc atgctgttct gcctgtaaca tacaatagca tccgaacatg gctgccccgg
      841 acaggattcg tctaggctcg taggcaacag taaccttgtc tactgcactg cagtttgtag
      901 gatgggtggt gtgtgagtcc acggtgcact gcaaccttca ggccggatgg ccagctgatg
      961 cctctgagta cctgtgggtt ccgtgactac agcctccact tctgtcctca gtccttgtac
     1021 atctgcaacc tgaaggcccc accgccaagc tacagggtga cctcatttga cgtgatgatg
     1081 gcattgctgt tgaggtgaca gaagccactg ttgtgtaaac taaaaataat catgagagag
     1141 aaaccagaat tcctatgatc actaacaatt aaaatatttt gttgtcttaa aaaaaaataa
     1201 aaggagaaat attttcctac aagagtactg aagtcaggaa cggatggagc ttctagccag
     1261 tgtattccgt ccagtaaaac agcattgcag ggagactctg ctcagctgac acgtttctag
     1321 ttttcaaatt gatgcctaat cgtagatgtt atcctaattc gtgacatgaa actcactcac
     1381 atatcagtcc tcaatagagt aaaagtcaga acaaggctgt gtgaagatcc agtctggctt
     1441 tagaatctgt tgtatcacct gctctgccac agaaaatagt ctctcatagg ggtttatgaa
     1501 aattgagtcg cacacccacc ctctgttaat ggagagagga tggtctttat ttactgaaca
     1561 agacagaagc aactgagatg gagagccagt cttactaacg agccttgtag ccgattaacc
     1621 atcgcagaag gaaaatgaag ccatgttaca cccacacgct cccgtttgca gacaccccac
     1681 aggacagtac agacatctgg ctttgtttgt gcgtgcgtgc gtgcgtgtgt gtgtgtgtgt
     1741 gtgtgtgtgt gtgtgtgtgt gtgttcgtga aagctgcctt taagggccac ggttgcaatg
     1801 ggacccttgg ctcacctgct ttggaaatcc agtctgctgt atcacagctt tattataggc
     1861 acttaaaaca aaaacaaaaa acagattgaa caacctatga aaaatttggg cttaaaagta
     1921 gagtataaat tattttagcg agggaaggaa gggacacaaa acctcttatc ttccctttcc
     1981 tggagaatca cggaagcttt tcatgtgtgc attccgccct gatggccgga gtggctggca
     2041 cacactgcct gagtgagtct tctgtttcga aagggtgctt aagctctggc atttttggcc
     2101 atgaagtgtt ttatcatttg catgagtaat ggcacaggaa aaaaaatttc agttcaggag
     2161 tttcaggagt gtggaagggt tcttctcacc tcccttagcg gcgtaagtgc tggcgagcgt
     2221 cttgatcctg ccctctgaag agcagtgtgg ccttcagaac cgcagtgtca gtgagcctgg
     2281 aggcacttag ggcctacgcg ttctcaggct ccacagtctc gtgcaagtaa ccacgtagtc
     2341 tcacgtgtac cagaaagaag agtgtgtttg tgtgtgcatg tgtgtttatc gttcctaaga
     2401 atttggcaca attcaggcta tcgcctcgac tgagaatcta tactgcagaa cataatgtat
     2461 aagcaagcag tttttaaatt attagtctct tacaagccat tggaatctcc cccaccccaa
     2521 gtatttagta gctggcttta agtttacaag atagggggac aaggtcaatg gtgtttgtca
     2581 tatgtgagaa aaaaatgata atgtgggtaa aaccaggttg tgggtgttag agagtcttag
     2641 aaaattcatg atcttcagcg ccattttctc cacttaggaa ggacaaaaat gtccatttta
     2701 taggacaaaa agtcctaaca gttttcttga aacaaagtct attatgtaaa agttgatttt
     2761 tgactcagac atattgaaaa attgaactaa agaagtatgt ttcatctgct gaagtagaaa
     2821 tagtgtgaga cctctgagac ctctaacttg tctgactcat tctaattttt agaatctcat
     2881 aatgaacagc aattgatgat actgcgtatt agaaggggct gcacagatcc cacagcctca
     2941 cgtcacggat ggcccatgca aacatggaaa gggtgtgaag cctgagaaag ccgggcaagg
     3001 tgtgaagtgg gagcttttgc caggcatctg ggcaggctct tcctaaggcc cagtgtttac
     3061 accagccctt catagtgcct gggcatcact tccccaggcc aaactgtacc acgccctctc
     3121 cctgttcctc tggtgcaagt ctgactgaca gttgtgcatg atggtgctga gatggggata
     3181 aaaacccaag gacaggaaaa ggacacttgt cagcacaaga aatggcaggg cgcaaaggag
     3241 agctggccca gtgatatttc tttaaaagcc caatccacct agcaaccaga taaatgaaag
     3301 tttgttctaa catgccttaa aaatatggtt tgaccccaaa gacccacaat ttctttagct
     3361 attacttagt tttctttgct ttgttttttt acttttcaca cagagccagg tttttgtttt
     3421 gttttaggtt tatttctttt atggtgtaac ttgcgggttc ttttaagctg tggcagcggc
     3481 ggtaaccaac gcctttctgc tcctcagctt ccacaggaca agccagcatg tgctgggaag
     3541 ccttctaggt gtttctgtac accgtgagag gtgctttgaa ctcccaccct ttgctttgta
     3601 gaattagggc ttgcccgtgc actccagttg ccactctctg gggtagaact actgatgctt
     3661 gcttgctttt tgtgtcgccc gtggacggct gtggtgcgcc gtccagtgtg agtgctgctg
     3721 ctggtggctg tgaaggtgtg ttgtcctcca ctgctgttgg gttgcttagt cgcttagttt
     3781 ctccatcagt agcccccctc ccccgttagc actggctcag ctttaactgt atcctcagcc
     3841 aatcagatgt tgaagaccat gggggtcctc ttgtttaatt tgtcctcgtg tatctattaa
     3901 accgtgatca gggtttctag aacggctgca gtagattttg aaaacagact tactgttatt
     3961 gattggggtt ccccagagat ccagttcaca gttaattgtt ggactctaat acaactgacc
     4021 attgaaaaat gaacaacggc ttattgtttt gtaacagtgt cgggtgagcc ctgacatttc
     4081 aattgaaaca tgaattgtca ttagaactca atgcaaattc aacagttgta tttggagtta
     4141 aattatttta acaaataaat ttatttaatg aaaa
//
