LOCUS       NM_175211               6341 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Ral GEF with PH domain and SH3 binding motif 1
            (Ralgps1), transcript variant 1, mRNA.
ACCESSION   NM_175211
VERSION     NM_175211.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Omidvar,N., Pearn,L., Burnett,A.K. and Darley,R.L.
  TITLE     Ral is both necessary and sufficient for the inhibition of myeloid
            differentiation mediated by Ras
  JOURNAL   Mol Cell Biol 26 (10), 3966-3975 (2006)
   PUBMED   16648489
  REMARK    GeneRIF: Ralgps1 is both necessary and sufficient for the
            inhibition of myeloid differentiation by Ras.
REFERENCE   3  (bases 1 to 6341)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ112478.1, AK076767.1 and BC072656.1.
            
            On Mar 14, 2014 this sequence version replaced NM_175211.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.121775.1, AK076767.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                CJ112478.1         2-17
            17-2068             AK076767.1         2-2053
            2069-6341           BC072656.1         1794-6066
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.25 cM"
     gene            1..6341
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /note="Ral GEF with PH domain and SH3 binding motif 1"
                     /db_xref="GeneID:241308"
                     /db_xref="MGI:MGI:1922008"
     exon            1..225
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            226..346
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             290..2047
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /note="isoform a is encoded by transcript variant 1; Ral
                     guanine nucleotide exchange factor RalGPS1A; ras-specific
                     guanine nucleotide-releasing factor RalGPS1; ralA exchange
                     factor RalGPS1; ral GEF with PH domain and SH3-binding
                     motif 1; ral guanine nucleotide exchange factor 2"
                     /codon_start=1
                     /product="ras-specific guanine nucleotide-releasing factor
                     RalGPS1 isoform a"
                     /protein_id="NP_780420.1"
                     /db_xref="CCDS:CCDS15939.1"
                     /db_xref="GeneID:241308"
                     /db_xref="MGI:MGI:1922008"
                     /translation="MYKRNGLMASVLVTSATPQGSSSSDSLEGQSCDYASKSYDAVVF
                     DVLKVTPEEFASQITLMDIPVFKAIQPEELASCGWSKKEKHSLAPNVVAFTRRFNQVS
                     FWVVREILTAQTLKIRAEILSHFVKIAKKLLELNNLHSLMSVVSALQSAPIFRLTKTW
                     ALLNRKDKTTFEKLDYLMSKEDNYKRTRDYIRSLKMVPSIPYLGIYLLDLIYIDSAYP
                     ASGSIMENEQRSNQMNNILRIIADLQVSCSYDHLTTLPHVQKYLKSVRYIEELQKFVE
                     DDNYKLSLRIEPGSSSPRLVSSKEDLAGPSAGSSSARFRRRPTCPDTSVAGSLPTPPV
                     PRHRKSHSLGNNLMCQLSVVESKSATFPSEKARHLLDDSVLESRSPRRGLTHTSSTAI
                     TNGLSLGSSESSEFSEEMSAGLESRGRLYATLGPNWRVPVRNSPRTRSCVYSPTSPCT
                     CTVGSSATVPTMEGPLRRKTLLKEGRKPALSSWTRYWVVLSGATLLYYGAKSLRGTDR
                     KHYKSTPGKKVSIVGWMVQLPDDPEHPDIFQLNNPDKGNVYKFQTGSRFHAILWHKHL
                     DDACKSSRPQVPANLMSFE"
     exon            347..454
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            455..505
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            506..589
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            590..679
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            680..772
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            773..899
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1037
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1131
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1199
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1326
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1484
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1536
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1620
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1726
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1819
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1925
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2017
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..6308
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2740..2745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /note="hexamer: AACAAA"
     polyA_site      2757
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
     regulatory      6287..6292
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /note="hexamer: ATTAAA"
     polyA_site      6308
                     /gene="Ralgps1"
                     /gene_synonym="5830418G11Rik; mKIAA0351; RalGEF 2;
                     RALGEF2; RALGPS1A"
                     /note="major polyA site"
ORIGIN      
        1 gagccggccg ccgctcaggc ccaggagtgg acggttccta ctgcggctgg gcaccggctg
       61 ggctcccgcg tcggcctcgc gtttcaagct tgcgcctggc tcaacctcaa tccggccccg
      121 gtctcggaac gaaggcgccg ctgccgctgg gcctctcgca agaccatgaa gaagcggtgg
      181 cagccactgc ggccctcgtc aaggtgacca gcccggacta cagcggacct ctcagacagg
      241 ttatgtcacc agcagaggtg gcgccgaagt tccctgcggc ctggagacta tgtacaagag
      301 gaatggtctg atggctagcg tgttggtcac ctctgccact ccacagggca gcagcagctc
      361 agactcgctg gagggccaga gctgcgacta tgccagcaag agctacgatg ctgtcgtctt
      421 cgacgttctg aaagtgactc ccgaggagtt tgccagtcag atcacattga tggacatccc
      481 cgtgttcaaa gccatccagc cagaggagtt ggccagttgt ggatggagta aaaaggagaa
      541 acacagtctt gcccccaacg tcgtggcctt tacccggagg ttcaaccagg tcagtttttg
      601 ggtcgtacga gaaattctaa cagcacagac tttaaaaata agggcagaaa tcctgagcca
      661 ttttgtgaaa atagccaaga aacttctaga actgaacaac cttcattctc tcatgtctgt
      721 ggtgtcagca ttacaaagtg ctcccatctt caggctgaca aaaacctggg ctcttttgaa
      781 tcgaaaagac aagactactt ttgagaaact tgactacctg atgtccaaag aagacaacta
      841 caagcgaacc cgggactaca tccgaagtct gaagatggtg cccagcattc cctatctagg
      901 aatctacctt ctggacttga tctacattga ttctgcatac cctgcctctg gcagcatcat
      961 ggaaaacgaa caacgatcca accagatgaa caacatcctc cgaatcattg ccgatttaca
     1021 agtttcctgt agctatgatc acctcactac cctgccacat gtgcaaaagt acctgaagtc
     1081 tgtgcgctac atcgaggagc tccagaagtt tgtagaagat gataattaca agttgtcact
     1141 cagaatcgaa ccaggaagca gctctccaag actagtttcg tccaaggaag atcttgcagg
     1201 cccctctgcc ggctccagct cagccaggtt ccgccggagg cccacctgtc cagatacatc
     1261 tgtggccggc agcctcccca caccgccagt ccccagacac aggaagagcc acagcctagg
     1321 caacaatctg atgtgtcagt tgagtgtagt tgaaagtaag agtgcgacat tcccatcgga
     1381 gaaggccagg cacctgctgg acgacagtgt cctagagtcc cgcagccccc gcaggggcct
     1441 cacccacacc tcctccaccg ccatcaccaa cggactctcc ctaggcagta gtgagagctc
     1501 agagtttagt gaagaaatgt ctgcagggct ggaaagcagg ggacgtcttt atgccacgct
     1561 gggacctaac tggcgggttc cagttcggaa ttctcccaga actcggagct gtgtctacag
     1621 ccccacgagc ccttgcacct gtactgtggg aagctcagcc actgtgccca ctatggaggg
     1681 gcctctgaga cggaaaaccc tgctcaagga aggccggaag cctgcactgt cctcgtggac
     1741 caggtactgg gttgtactct caggagccac cctcctttac tatggagcca agtctttgcg
     1801 gggtacagac agaaaacatt ataaatctac acctggcaag aaggtctcca tcgtaggctg
     1861 gatggtgcag ctgccagatg accccgagca cccagatatc ttccagctga ataaccctga
     1921 caaaggcaat gtttacaagt tccagacggg ctcccgtttc catgcgatac tgtggcacaa
     1981 gcatttggat gatgcatgta aaagcagcag gcctcaggta cctgcaaacc ttatgtcctt
     2041 tgaatgagta tctgcaggac ttggcgagac tgcaaaggct tctgagagcc tagactaggt
     2101 ctgtggagga gagcagctct gggcataagc tgtgggccaa ggcactggag cctcatcgct
     2161 ggactcgagg aatatggcca tggcctcaca gtcctggatg gctccccagt gtgagatcca
     2221 cctgtcaacc cacagcaact ctcatgaatc attctgcagc agcaccccac cgggtggctg
     2281 tcagacccca aactgcgcac cctatgcccc ctctctgggc catcgtctgt ccaccagctg
     2341 cttttgcatc aggggagcat ttgacccatg ttgggttctc catgctttct gctgcacaca
     2401 gagtggatgg cgttaatctt cgagagggct ggaaagcaga gacgggctgc tggacaggct
     2461 tgttacaccc caagtgcacc ggccactcac ccacagggcc gcctccgatt ttgtataccc
     2521 ttaaagcatc ctgtgtgtgt gtgcgcgcgg tgtgtgtgtg tgagcgagtg agtgtgaacg
     2581 ctctgagaaa catacatttt ggcacaagac ttgtgacatc acacacttat tggctttgag
     2641 tccctgcttg aagctttagt tacagccttg tcctctaaga aaagtctgaa gggagtccag
     2701 aagctccgtg ttaagggtcc cttgagttct tttactgtaa acaaacaatg ccttaaatcg
     2761 tgtcttgttt tctgttccta tgggtgctat tcatctggat ggcctgttcc ctgggcccca
     2821 ggccctcctg ggtcttgttg tcagcccgcc tgaggcagaa tccatttcag gaccgtgtac
     2881 tgggcactgg cctgcttgct ccccaccacg agctttcctg gtggtcctat gggccctcct
     2941 tatctcgccc actcctgcta tgctggtgtg ggaagctggg accttttctc ccagcagcgt
     3001 ggaggccccc acagtgaccg gtggtcagaa gctgtgccac cccgcattct cccacggggc
     3061 agaattacgc tcctttcctc acccttgcca ccactgccag ggccggccac actgattcct
     3121 gatcacacag ggagctgagt gacaaggggg cctaagcccc ctcagtcttg cccctaaatg
     3181 cagtcaccag caagttctca tccaagtcca agggcccagt tcttgatgac caggtgagaa
     3241 gagagccaaa ccctagggag tgagatgtcc agcctctgca ttcatgaaca aggacctatc
     3301 tgtcacctgt tgtcataaat aaggtgattt ttactttgca cattttaatt caagtgtatt
     3361 gccctttgtc atcactgatg ctctctgtcc ttcttcctct tcctcttcct ccttgaatct
     3421 gggtacccta accctgagaa gaaaaccctc agttttctgg aaaaggctga atgtctgggt
     3481 ccctgtgggt atcatggttt tacacagtaa cccaatcatg aaaccatccc aacaataatg
     3541 gagaagctgc agacaacacc ataaccatga acttaccacc agaaagcact gtattacaaa
     3601 caaatggtga catgtctcta aaacatggat gacttgatgg ggtgattagt tgttgtcatg
     3661 gccgtacgtt tgttgtactc tgaagctagc cttcctgtgg gcagagcctg gatattttca
     3721 tgccaagaga aactccacat ggtaaagttg atgccatcaa accttgacca cagctgcctt
     3781 gcacatccac ccccaccagt ggtccagctg tgtgcactag ctgtatgcac tggattctat
     3841 caacgttgct ccagttagaa tccagaagat ggatgacggt gtcagacact tgaggcacct
     3901 ccaagcagaa ccattcgtgg tccactttaa tctattccaa ctccaaacct gcctgcctgc
     3961 ctgcctgtta actcctagaa agccagacca tcagctgcta gtttccttta ggtgtctgct
     4021 tttgggctca ttgtttggat tcttgcatgt gcagtgtcaa gacatcaaaa ccccagaact
     4081 tcatcccact caaataactt cctgcccttt acagatttgt cttatgagac acagtgtttt
     4141 cttccaggcc taatctgaaa gagcacaatc acaattattt tcaaagtgtt taatcttcct
     4201 aaatgaagcc aaactcccct cccacaagca tccttaccat gaactcttga agacaactgc
     4261 ttgaacttta ttagtgaaaa gccttgtagg acttttgtga gccttgaagg tagtgggaaa
     4321 gccctgtcta tggagttaaa agatatttgt ctctccaagc agggccttag gccaagcata
     4381 caagctcagt gctgatatcc tcggcacagc ttgtcacaca aggctgcaag gggaggtggc
     4441 caggtcacat gacagagtca gtggtccttt ggtggactct tagccttgaa ggaagctgtt
     4501 ctgcactttt gctccactgt ccatcgtaca caaatactgt gaaactgttg gagacagacc
     4561 atggcacccc aaactgagag ctgtagaatg ctgtttagaa actgggatgg gcagctggta
     4621 gaattaggaa gtcccaagaa aagccttctc actcacaata agagtaaacc tctgtcccct
     4681 ttccagtgta caagttttgc aatacaaggt cagccaagtg tccgctggca agcaagatac
     4741 cttgtggatg ctggacttgg tgggaaacac tcctacacaa gccagcagga gagtccggcc
     4801 ttcccctgcc ctggctgaat gccttggggc acttgatgct gagcaggtag acacatccgt
     4861 ggctcgccaa caggctctct gtatttctcc tccttaccaa gatggccagt gtttcttcca
     4921 tcagtttact cttgcctctc gagcctaaga aaggtgtggt ggttttttgt ttgttttgtt
     4981 tattttttaa tatcaatttt cagatggaaa atgatctgta aaactgggaa gccatttata
     5041 agccaaggct gctttaatct gcacgtggca tccagagctt tgcaacctct attgaatatt
     5101 gccaaaagca cagcttctct tctgtgtgac ccttaaactg tccctgtgtc atattgatat
     5161 gttgggtgat ttcatgttat aaaacaaaca aaaaacccac cctgcacaaa gcccttttga
     5221 aagtgtattt attgcataaa cattcatatg tcaagtgaat acttcttata ccccaggctc
     5281 tacccagact gaaaaaggct agaaacaaga caaatccaat tttggccttt gtagaactta
     5341 accagtcaat gaccaaataa ttaccacctc acaagccaac tttggcaaga ccctatctct
     5401 cccctactcc tgaatacaat cgtgtgcatt ggttcttcca ggaagaccag attgaatgga
     5461 aatgttggga gagtaagttg cattgaggac ttgctggagt cccgacatgg gaggctggct
     5521 ggctctaagc agccagattc tgatctgtgt tcaagaatgg ttccagggaa gacgccaaag
     5581 gcaggatgag agagtagggt ctgagtgctt gccgtacgca cagcccccca ccccgtttac
     5641 tctgtaactt cagtgttaat tcatatacag tatgagtggg agctgcgtgt ccctgggagc
     5701 cgaggacttg gactttggtc catcttgtaa gctgctgctg ctgcacaagt gtgtgtccca
     5761 tggacagccc caaattgcca ctcaccgcag caaggtgata ggtcatctgc cattttggaa
     5821 ggactttgac cattcagtaa tgaaataata gtaattcatt tattagtgag gcatactctt
     5881 taataaattt tgtgccaaaa tgcatggttt cccacttagc attcaaaatg ttgtataaag
     5941 agtagttttc aatttcttac atgttcttcc aagtaaactg aaaatgagtt ttcacatctt
     6001 actttgtttc ttgttcaatc tgctgcaccc ccccccttaa gctgtctttt tttcccagca
     6061 gaccctgcat gcagttgtgt gaggactttt ctctaacatt tgtaaattgt ctcatctgca
     6121 ataaccactg aacatcatcc ctccttggga gtctttagat ttttggtgaa gtattttgtt
     6181 acctcagtct tgtatcaagt tgctgtattt ttcagcttgt tacactgata ataattgttt
     6241 cactaattaa atactttaat gtacagacat ctttgtttac tttgaaatta aatgtgtttt
     6301 ccaatgaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//
