LOCUS       NM_001408200            6409 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus Ral GTPase activating protein, alpha subunit 2
            (catalytic) (Ralgapa2), transcript variant 2, mRNA.
ACCESSION   NM_001408200 XM_006499517
VERSION     NM_001408200.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Iida,T., Hirayama,D., Minami,N., Matsuura,M., Wagatsuma,K.,
            Kawakami,K., Nagaishi,K., Nojima,M., Ikeuchi,H., Hirota,S.,
            Shirakawa,R., Horiuchi,H. and Nakase,H.
  TITLE     Down-regulation of RalGTPase-Activating Protein Promotes
            Colitis-Associated Cancer via NLRP3 Inflammasome Activation
  JOURNAL   Cell Mol Gastroenterol Hepatol 9 (2), 277-293 (2020)
   PUBMED   31622786
  REMARK    GeneRIF: Down-regulation of RalGTPase-Activating Protein Promotes
            Colitis-Associated Cancer via NLRP3 Inflammasome Activation.
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Uegaki,M., Kita,Y., Shirakawa,R., Teramoto,Y., Kamiyama,Y.,
            Saito,R., Yoshikawa,T., Sakamoto,H., Goto,T., Akamatsu,S.,
            Yamasaki,T., Inoue,T., Suzuki,A., Horiuchi,H., Ogawa,O. and
            Kobayashi,T.
  TITLE     Downregulation of RalGTPase-activating protein promotes invasion of
            prostatic epithelial cells and progression from intraepithelial
            neoplasia to cancer during prostate carcinogenesis
  JOURNAL   Carcinogenesis 40 (12), 1535-1544 (2019)
   PUBMED   31058283
  REMARK    GeneRIF: loss of RalGAP function has a distinct role in promoting
            progression from prostatic intraepithelial neoplasia to invasive
            adenocarcinoma.
REFERENCE   3  (bases 1 to 6409)
  AUTHORS   Oeckinghaus,A., Postler,T.S., Rao,P., Schmitt,H., Schmitt,V.,
            Grinberg-Bleyer,Y., Kuhn,L.I., Gruber,C.W., Lienhard,G.E. and
            Ghosh,S.
  TITLE     kappaB-Ras proteins regulate both NF-kappaB-dependent inflammation
            and Ral-dependent proliferation
  JOURNAL   Cell Rep 8 (6), 1793-1807 (2014)
   PUBMED   25220458
REFERENCE   4  (bases 1 to 6409)
  AUTHORS   Saito,R., Shirakawa,R., Nishiyama,H., Kobayashi,T., Kawato,M.,
            Kanno,T., Nishizawa,K., Matsui,Y., Ohbayashi,T., Horiguchi,M.,
            Nakamura,T., Ikeda,T., Yamane,K., Nakayama,E., Nakamura,E.,
            Toda,Y., Kimura,T., Kita,T., Ogawa,O. and Horiuchi,H.
  TITLE     Downregulation of Ral GTPase-activating protein promotes tumor
            invasion and metastasis of bladder cancer
  JOURNAL   Oncogene 32 (7), 894-902 (2013)
   PUBMED   22450745
REFERENCE   5  (bases 1 to 6409)
  AUTHORS   Chen,X.W., Leto,D., Xiong,T., Yu,G., Cheng,A., Decker,S. and
            Saltiel,A.R.
  TITLE     A Ral GAP complex links PI 3-kinase/Akt signaling to RalA
            activation in insulin action
  JOURNAL   Mol Biol Cell 22 (1), 141-152 (2011)
   PUBMED   21148297
  REMARK    GeneRIF: study reports the identification and characterization of a
            Ral GAP complex (RG1, RGC2) that mediates the activation of RalA
            downstream of the PI 3-kinase/Akt pathway
REFERENCE   6  (bases 1 to 6409)
  AUTHORS   Gridley,S., Chavez,J.A., Lane,W.S. and Lienhard,G.E.
  TITLE     Adipocytes contain a novel complex similar to the tuberous
            sclerosis complex
  JOURNAL   Cell Signal 18 (10), 1626-1632 (2006)
   PUBMED   16490346
REFERENCE   7  (bases 1 to 6409)
  AUTHORS   Gridley,S., Lane,W.S., Garner,C.W. and Lienhard,G.E.
  TITLE     Novel insulin-elicited phosphoproteins in adipocytes
  JOURNAL   Cell Signal 17 (1), 59-66 (2005)
   PUBMED   15451025
REFERENCE   8  (bases 1 to 6409)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845430.12, AL845273.7 and AL935056.18.
            
            On Jun 14, 2022 this sequence version replaced XM_006499517.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.3229.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-270               AL845430.12        31370-31639         c
            271-381             AL845430.12        4697-4807           c
            382-434             AL845273.7         155814-155866       c
            435-492             AL845273.7         137151-137208       c
            493-536             AL845273.7         129846-129889       c
            537-714             AL845273.7         128120-128297       c
            715-830             AL845273.7         126889-127004       c
            831-969             AL845273.7         125647-125785       c
            970-1169            AL845273.7         123713-123912       c
            1170-1397           AL845273.7         120221-120448       c
            1398-1565           AL845273.7         112276-112443       c
            1566-1703           AL845273.7         111217-111354       c
            1704-1852           AL845273.7         108255-108403       c
            1853-1964           AL845273.7         105042-105153       c
            1965-2202           AL845273.7         101054-101291       c
            2203-2367           AL845273.7         98030-98194         c
            2368-2508           AL845273.7         94799-94939         c
            2509-2643           AL845273.7         89171-89305         c
            2644-2757           AL845273.7         88483-88596         c
            2758-2855           AL845273.7         86610-86707         c
            2856-2946           AL845273.7         82779-82869         c
            2947-3123           AL845273.7         81300-81476         c
            3124-3317           AL845273.7         64928-65121         c
            3318-3416           AL845273.7         64044-64142         c
            3417-3572           AL845273.7         63665-63820         c
            3573-3701           AL845273.7         37828-37956         c
            3702-3830           AL845273.7         34394-34522         c
            3831-3889           AL845273.7         33525-33583         c
            3890-3998           AL845273.7         29608-29716         c
            3999-4109           AL845273.7         24885-24995         c
            4110-4178           AL845273.7         23553-23621         c
            4179-4316           AL845273.7         23146-23283         c
            4317-4500           AL845273.7         21823-22006         c
            4501-5272           AL845273.7         18415-19186         c
            5273-5344           AL845273.7         16816-16887         c
            5345-5468           AL845273.7         10929-11052         c
            5469-5624           AL845273.7         9853-10008          c
            5625-5783           AL845273.7         3815-3973           c
            5784-5911           AL935056.18        135446-135573       c
            5912-6033           AL935056.18        80889-81010         c
            6034-6409           AL935056.18        79092-79467         c
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 71.33 cM"
     gene            1..6409
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /note="Ral GTPase activating protein, alpha subunit 2
                     (catalytic)"
                     /db_xref="GeneID:241694"
                     /db_xref="MGI:MGI:3036245"
     exon            1..270
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..6065
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /note="isoform 2 is encoded by transcript variant 2; ral
                     GTPase-activating protein alpha subunit 2; ral
                     GTPase-activating protein subunit alpha-2; 250 kDa
                     substrate of Akt"
                     /codon_start=1
                     /product="ral GTPase-activating protein subunit alpha-2
                     isoform 2"
                     /protein_id="NP_001395129.1"
                     /db_xref="GeneID:241694"
                     /db_xref="MGI:MGI:3036245"
                     /translation="MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLK
                     QFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFF
                     RWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFP
                     AVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKY
                     MVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPK
                     PVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKII
                     QTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQ
                     RILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILQNKPVFMEEPDKKDV
                     AQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGA
                     QAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQM
                     LQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFL
                     RVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCI
                     LEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATAKRSQS
                     ISNCVHLSEALPATKSVPLLLHTVSALLPGLSYSSCSHRLSEVEEFQQAESTAAADCD
                     YLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPL
                     PLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDD
                     LQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDAR
                     HWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQ
                     SPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTL
                     PNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNC
                     SPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPL
                     LQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSAS
                     HPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVAT
                     IAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAPL
                     LDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSAD
                     LGNLLTVEEEKKRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAH
                     VEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISG
                     KYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHTCGPQKDLFNGEEGDDVLDKLLEN
                     IGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSV
                     TVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQ
                     CRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRN
                     GSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRR
                     GIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCI
                     NASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSVSGTVK
                     EQEESTAS"
     exon            271..381
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            382..434
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            435..492
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            493..536
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            537..714
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            715..830
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            831..969
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1169
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1397
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1565
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1703
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1852
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..1964
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2202
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2367
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2508
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2509..2643
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2757
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2855
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2856..2946
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2947..3123
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3317
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3318..3416
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3417..3572
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3573..3701
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3702..3830
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3831..3889
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3890..3998
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3999..4109
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            4110..4178
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            4179..4316
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            4317..4500
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            4501..5272
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            5273..5344
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            5345..5468
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            5469..5624
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            5625..5783
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            5784..5911
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            5912..6033
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     exon            6034..6409
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6380..6385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /note="hexamer: AATAAA"
     polyA_site      6409
                     /gene="Ralgapa2"
                     /gene_synonym="A230067G21Rik; AS250; P220; pp250; RGC2"
                     /note="major polyA site"
ORIGIN      
        1 gcccctcctt cctggagcct ccgctgaggc tgaggcggcg aggcggccgg gaggggggcg
       61 gcttgtggcc ggagcgacac gcgcccgtcg cgaccctatg cagtgactcg tgcggcgcag
      121 gcgcctcctg ccccgccggc ggggcccagg cttaccgcgg cgggatgttc tcccgaagga
      181 gccacggaga tgtgaaaaag tccacgcaga aggtgctgga ccccaagaag gacgtgctga
      241 cccgcctgaa gcacctgcgg gcgctgctgg ataacgtgga tgcaagcgat cttaagcagt
      301 tctttgagac caactattct cagatatatt tcatcttcta tgaaaatttt ataacactgg
      361 aaaatagttt gaagttaaaa gggaataata agtcacaaag ggaggagctg gactccatcc
      421 tctttctatt tgaaaaaata ctgcagtttc tacctgaacg aattttcttt cgatggcatt
      481 accagagtat aggctcaact ttaaagaagc ttttacacac tggaaattct attaagataa
      541 gatgtgaagg aatcagacta tttcttttgt ggcttcaagc acttcagaca aactgtgcag
      601 aagagcaggt gctgattttc gcttgcctag tgcctggttt cccagcggtc ttgtcatcca
      661 ggggtccctg cacactggag acactcatca accctagccc tagtatagtt gatgcaaaaa
      721 tatacccaga ggaaatcacc ccacttctgc cagccatatc aggggagaag attgctgagg
      781 accaaacctg cttttttctt caaatactgt tgaaatacat ggttattcag gctgcaagct
      841 tggagtggaa gaataaagag aatcaagata ctggttttaa atttcttttt acgttgtttc
      901 gaaagtatta tcttcctcat ttgtttccat catttactaa attaaccaac atctacaaac
      961 ctgtacttga aattccccat ttgagaccaa agccagtata tgttactgta actcgagata
     1021 atgaaacaat ttacagtaca aaaattccat acatggcagc tcgtgttgtt ttcattaaat
     1081 ggattgtaac tttctttttg gaaaaaaagt atctaactgc aacacaaaac actaaaaatg
     1141 gagttgatgt attgcctaaa atcatccaga ctgttggtgg tggtgcaata caagagaagg
     1201 taccagagct ggatggtgct gggtccacag aacaggacaa aagccattct aacagcagca
     1261 ccttgtctga ccgaagactc agcaactcca gtctttgtag cattgaagag gagcatcgga
     1321 cagtgtatga gatggtgcag cggatcctcc tgtcaacacg gggttatgtg aattttgtga
     1381 atgaagtatt tcgacaggca tttttgttgc cttcctgtga gatatctgta acaagaaaag
     1441 tagttcaagt gtataggaaa tggatcctcc agaacaaacc tgttttcatg gaggaaccag
     1501 ataaaaagga tgttgctcaa gaagatgctg acaaattagg actttcagag actgactcca
     1561 aggaggcctc atctgaaagt tctggtcata aacggtcatc cagttgggga cgcacgtatt
     1621 ccttcacaag tgccatgagc agaggatgtg tgacagagga ggacaacaca aatgtgaaag
     1681 ctggggccca agctatgttg caggtctttc ttacgaatgc tgcaaatgtc tttttgctgg
     1741 aaccatgtgc cgaagttccc atgctcctga gagaacaagt agacgcctct aaagctgttc
     1801 tgattatttt tcggcggatg ataatggagc ttacaatgaa tcaaaagaca tgggagcaga
     1861 tgttgcaaat ccttctcagg ataacagaag ctgtcatgca gaagccaaag gataagcatg
     1921 tcaaggactt gtttgcccag agcttggcag ggttgctctt caggacactt attgtggctt
     1981 ggatccgagc aaacctctgt gtgtacattt ctcgggagct ctgggatgac tttctcaggg
     2041 tgctgtcatc ccttacggaa tgggaggaac tcatcactga atggtctaac atcatggatt
     2101 ccttgacagc cgtgcttgcc agaactgtgt atggagttga aatgacaaac ctacctctag
     2161 ataagttaag tgaacaaaag gagaagaagc agcgtggcaa aggttgtatc ctagaacccc
     2221 aaaaaggaac agctgttggg agatcctttt ctctcagctg gcgtagccat ccagatgtga
     2281 ctgagccaat gagattcaga agtgccacca catctggagc accaggagtg gagaaggcaa
     2341 gaaatactgt ccgtcaaaaa gcaactgcta agcgaagcca gtctatcagc aactgtgtcc
     2401 acctctctga ggctttgcct gctactaaaa gcgtcccgct cctcctccat accgtgagcg
     2461 ctctcttacc tggtctctct tactcctctt gctctcacag gctgtcagaa gtggaggagt
     2521 tccagcaggc agagagcacc gccgcagccg actgtgacta tcttgtggta ggacagcagc
     2581 aggtgccccg gagcagcagc acctctgaca tcactgagcg cttgtactca gactcctctc
     2641 aaggttacag ccccatgttg cctcatcctg ctttttccac gacttctggg gatcagagct
     2701 ctggtcctca tgcttgcaca gctggccctt tacctttgag ccgtctcccc agtgcaggtc
     2761 aaaaggtaga acattcgcag aatttaagct cttcagaacc caagtctgtt caagagagta
     2821 aaggacatgt gactcatgaa catgaaggaa taacaatgtt ggttcgaagg agcagcagtc
     2881 ccgctgagct ggagctgaag gatgatttac agcaggcaca tggaagatgc aggcagagac
     2941 agacaagtga aagtactggt agtgacacag ttgtgggcta cagcaatgaa gcagagctac
     3001 ctgtgagccc gtggcaggcc tgtgaggagg acccggacct gagcactccc acagatgctg
     3061 tggctgactc tgatgcccgc cactggttac aactgagtcc tactgatgct tcaaacttaa
     3121 cagacagcag agaatgcctt gcagacgact gtagcattat tgctggagga aacctcactg
     3181 gttggcaccc agactccgct gctgtgttat ggcgaagagt cttgggaatc cttggagatg
     3241 tgaataacat ccagtcgccg aagatccatg ccaaagtttt tggctatctc tatgaactgt
     3301 ggtataaact ggccaagata cgagataatt tagcaataag cctggataac cagtcgtctc
     3361 catctcctcc tctcttgatc ccaccactca gaatgtttgc atcatggcta tttaaggcta
     3421 caactctgcc aaatgaatat aaagaaggca aactgcaagc ctacaaactg atctgtgcca
     3481 tgatgaccag acgccaggat gttctgccaa actctgactt cctggtgcat ttttaccttg
     3541 tgatgcacct gggactaacc agcgaggacc aggatgtctt aaacaccatc ataaaaaact
     3601 gctcaccccg ctttttctcc ctgggtttgc ctggcttctc tatgctggtg ggggacttta
     3661 tcactgctgc tgccagggtc ctcagtacag acatgttggc ggcaccccgt tcagaagctc
     3721 tcactctcct tggttccctt gtctgcttcc caaataccta ccaggagatc cctctgctgc
     3781 agtcagtgcc agaagtgagt gatgttgtca cgggagcgga agacgtcaag cattacctca
     3841 taaatatttt actgaagaat gctaccgagg aaccaaatga atgtgcaaga tgcatcgcca
     3901 tttgctccct cggggtctgg atctgtgaag agcttgcaca gagtgccagc catcctcagg
     3961 tgaaggatgc catcaatgtg ataggtgtaa ctttgaagtt tcctaacaag atcgtggctc
     4021 aggtagcctg tgatgttctt cagttgttgg tttcctactg ggaaaagctg cagatgtttg
     4081 aaactgctct accccgcaaa atggcagaaa ttcttgtggc cacaattgcg ttccttttac
     4141 caagtgcaga gtactcgtca gtggaaacag ataaaaagtt tattgtgtcc ttgctccttt
     4201 gtctcttgga ctggtgcatg gcgctgcccg tgagtgccct cctccaccct gtgtccacag
     4261 cagttctaga ggagctgcac ccatcccggg cccccttgct ggattatatc tacagggttc
     4321 tgcactgctg tgtctgtggc tccagcacat acacacagca aagtcactat actctgacct
     4381 tggctgacct gtcatccacg gattatgacc ccttcctacc tctggcaaac gtgaggaact
     4441 ccgagccaat ccagtatcat tcatcagcag acctggggaa cctgctgacc gtggaagagg
     4501 aaaagaagag aagaagtgtg gaattgatcc ccctcactgc acgaatggtc atggcccacc
     4561 tagtgaacca cctaggccac tatccactga gtgggggtcc tgctgtgctg cacagcctgg
     4621 tcagtgagaa ccatgacaat gcccatgtgg aaggaactga gctgtcctcg gaggtgttca
     4681 ggagcccaaa cctgcagctc ttcgtattta atgatagcac cctcatctcc tatcttcaga
     4741 caccagcaga aggaccagct ggagggactt cagggggttc cctctcagat gtgagagtca
     4801 ttgtgaggga tatctcaggg aagtactctt gggatggcaa ggttttatat ggacctctgg
     4861 aaggccgttt ggcacccaat ggaaggaacc cctcatttca gatttctggc tggcatcatc
     4921 acacatgtgg acctcagaaa gatttgttta acggtgagga gggagatgat gtgcttgaca
     4981 agttacttga aaacattggc catacaagcc ccgaatgcct tttaccatca caactaaatc
     5041 taaatgagcc ttccccaacc ccatgtgcaa tgaactggga ccaagagaag gcaatcatgg
     5101 aggtcatcct gcgccagagc gcacaggaag atgagtatgt gcagaggtgt aactcggatt
     5161 cttcagtaac ggtcaccagc caagggcagc cctcacccgt ggagccccgg ggaccctttt
     5221 atttctgcag gttgttgctt gatgacttgg gaatgaattc ctgggacaga aggaagaatt
     5281 ttcatttgtt gaagaaaaat tcaaaattat tgagagagct aaagaatctg gactcgcgcc
     5341 agtgccgcga gacccataag attgcagtgt tttacattgc tgaagggcaa gaagacaagt
     5401 gttccattct agccaatgaa agaggaagcc aagcatatga agactttgtt gctggtcttg
     5461 gatgggaggt ggatctctca acccactgtg ggtttatggg tggtcttcag cgcaatggca
     5521 gcacgggaca gacagcccct tactatgcta cctcgaccgt ggaagtaatt ttccacgttt
     5581 ccactcggat gccatcagat tcagatgatt ctctcaccaa aaagctccgt cacctgggga
     5641 atgatgaggt ccacatcgtc tggtctgaac actccaggga ctaccgcagg ggtattatcc
     5701 caacagcctt tggagacgtt tccatcatta tttacccaat gaagaatcat atgttcttta
     5761 tcacgataac gaagaagcct gaggttccat tctttgggcc cctgttcgat ggagcgatcg
     5821 tgagtgggaa gctgctgcca agccttatct gtgccacatg catcaatgcc agcagagcgg
     5881 tgaagtgcct catcccactc tatcagagct tctacgaaga gcgagctctg tatctggaag
     5941 caataattca aaaccaccgg gaagtcatga catttgagga tttcgctgct caagtctttt
     6001 ctccctctcc cagctactct gtcagtggaa cggtgaaaga acaggaggaa tccacagcaa
     6061 gctaatgctt tccagctcca ttcagtctct tcccatcctt tggagacgct gggactcggc
     6121 gtttctcagc agaggctttg gttttgggtt tgtttcttgg tttttttttt ttttgttttt
     6181 ttttgttttt ggttttgttt ttgttgtttt tttttttttt taacttctaa gaaaacattt
     6241 catttcttaa aaataaaaga aaaaaatgaa atgataaatg tttatatttc ctaacttttt
     6301 atttttaaag ttgatgatga tcaatgttat tgaaataatg atggctgtta ttttctatgt
     6361 atattttagc ctctgtataa ataaagttca accaattcag ttcttagga
//
