LOCUS       NM_009019               6669 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus recombination activating 1 (Rag1), mRNA.
ACCESSION   NM_009019
VERSION     NM_009019.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Biggs O'May,J., Vanes,L., de Boer,L.L., Lewis,D.A., Hartweger,H.,
            Kunzelmann,S., Hayward,D., Llorian,M., Kochl,R. and
            Tybulewicz,V.L.J.
  TITLE     WNK1-dependent water influx is required for CD4+ T cell activation
            and T cell-dependent antibody responses
  JOURNAL   Nat Commun 16 (1), 1857 (2025)
   PUBMED   39984435
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Isshiki,Y., Chen,X., Teater,M., Karagiannidis,I., Nam,H., Cai,W.,
            Meydan,C., Xia,M., Shen,H., Gutierrez,J., Easwar Kumar,V.,
            Carrasco,S.E., Ouseph,M.M., Yamshon,S., Martin,P., Griess,O.,
            Shema,E., Porazzi,P., Ruella,M., Brentjens,R.J., Inghirami,G.,
            Zappasodi,R., Chadburn,A., Melnick,A.M. and Beguelin,W.
  TITLE     EZH2 inhibition enhances T cell immunotherapies by inducing
            lymphoma immunogenicity and improving T cell function
  JOURNAL   Cancer Cell 43 (1), 49-68 (2025)
   PUBMED   39642889
REFERENCE   3  (bases 1 to 6669)
  AUTHORS   Oka,T., Smith,S.S., Son,H.G., Lee,T., Oliver-Garcia,V.S.,
            Mortaja,M., Trerice,K.E., Isakoff,L.S., Conrad,D.N., Azin,M.,
            Raval,N.S., Tabacchi,M., Emdad,L., Das,S.K., Fisher,P.B.,
            Cornelius,L.A. and Demehri,S.
  TITLE     T helper 2 cell-directed immunotherapy eliminates precancerous skin
            lesions
  JOURNAL   J Clin Invest 135 (1), e183274 (2025)
   PUBMED   39744942
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6669)
  AUTHORS   Xiao,X., Du,Y., Sun,S., Su,X., Xing,J., Wang,G., Elzein,S.M.,
            Zou,D., Minze,L.J., Mao,Z., Ghobrial,R.M., Connor,A.A., Chen,W.,
            Zhang,Z. and Li,X.C.
  TITLE     Apex1 safeguards genomic stability to ensure a cytopathic T cell
            fate in autoimmune disease models
  JOURNAL   J Clin Invest 135 (4), e183671 (2024)
   PUBMED   39739423
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6669)
  AUTHORS   Yamashita,M., Ogawa,C., Zhang,B., Kobayashi,T., Nomura,A.,
            Barker,C., Zou,C., Yamanaka,S., Hayashi,K.I., Shinkai,Y., Moro,K.,
            Fargarasan,S., Imami,K., Seita,J., Shirai,F., Sawasaki,T.,
            Kanemaki,M.T. and Taniuchi,I.
  TITLE     Cell-type specific, inducible and acute degradation of targeted
            protein in mice by two degron systems
  JOURNAL   Nat Commun 15 (1), 10129 (2024)
   PUBMED   39613744
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6669)
  AUTHORS   Oettinger,M.A.
  TITLE     Activation of V(D)J recombination by RAG1 and RAG2
  JOURNAL   Trends Genet 8 (12), 413-416 (1992)
   PUBMED   1492366
  REMARK    Review article
REFERENCE   7  (bases 1 to 6669)
  AUTHORS   Mombaerts,P., Clarke,A.R., Rudnicki,M.A., Iacomini,J., Itohara,S.,
            Lafaille,J.J., Wang,L., Ichikawa,Y., Jaenisch,R., Hooper,M.L. et
            al.
  TITLE     Mutations in T-cell antigen receptor genes alpha and beta block
            thymocyte development at different stages
  JOURNAL   Nature 360 (6401), 225-231 (1992)
   PUBMED   1359428
  REMARK    Erratum:[Nature 1992 Dec 3;360(6403):491]
REFERENCE   8  (bases 1 to 6669)
  AUTHORS   Brandle,D., Muller,C., Rulicke,T., Hengartner,H. and Pircher,H.
  TITLE     Engagement of the T-cell receptor during positive selection in the
            thymus down-regulates RAG-1 expression
  JOURNAL   Proc Natl Acad Sci U S A 89 (20), 9529-9533 (1992)
   PUBMED   1329099
REFERENCE   9  (bases 1 to 6669)
  AUTHORS   Mombaerts,P., Iacomini,J., Johnson,R.S., Herrup,K., Tonegawa,S. and
            Papaioannou,V.E.
  TITLE     RAG-1-deficient mice have no mature B and T lymphocytes
  JOURNAL   Cell 68 (5), 869-877 (1992)
   PUBMED   1547488
REFERENCE   10 (bases 1 to 6669)
  AUTHORS   Guy-Grand,D., Vanden Broecke,C., Briottet,C., Malassis-Seris,M.,
            Selz,F. and Vassalli,P.
  TITLE     Different expression of the recombination activity gene RAG-1 in
            various populations of thymocytes, peripheral T cells and gut
            thymus-independent intraepithelial lymphocytes suggests two
            pathways of T cell receptor rearrangement
  JOURNAL   Eur J Immunol 22 (2), 505-510 (1992)
   PUBMED   1537384
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK162974.1 and AL929569.13.
            
            On Mar 20, 2007 this sequence version replaced NM_009019.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK162974.1, BC138342.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-407               AK162974.1         1-407
            408-6669            AL929569.13        136954-143215       c
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 53.88 cM"
     gene            1..6669
                     /gene="Rag1"
                     /gene_synonym="Rag-1"
                     /note="recombination activating 1"
                     /db_xref="GeneID:19373"
                     /db_xref="MGI:MGI:97848"
     exon            1..111
                     /gene="Rag1"
                     /gene_synonym="Rag-1"
                     /inference="alignment:Splign:2.1.0"
     exon            112..6669
                     /gene="Rag1"
                     /gene_synonym="Rag-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..3248
                     /gene="Rag1"
                     /gene_synonym="Rag-1"
                     /EC_number="2.3.2.27"
                     /note="recombination activating protein 1; recombinase
                     activating gene 1; recombination activating gene 1"
                     /codon_start=1
                     /product="V(D)J recombination-activating protein 1"
                     /protein_id="NP_033045.2"
                     /db_xref="CCDS:CCDS16463.1"
                     /db_xref="GeneID:19373"
                     /db_xref="MGI:MGI:97848"
                     /translation="MAASLPSTLSFSSAPDEIQHPQIKFSEWKFKLFRVRSFEKAPEE
                     AQKEKDSSEGKPYLEQSPVVPEKPGGQNSILTQRALKLHPKFSKKFHADGKSSDKAVH
                     QARLRHFCRICGNRFKSDGHSRRYPVHGPVDAKTQSLFRKKEKRVTSWPDLIARIFRI
                     DVKADVDSIHPTEFCHDCWSIMHRKFSSSHSQVYFPRKVTVEWHPHTPSCDICFTAHR
                     GLKRKRHQPNVQLSKKLKTVLNHARRDRRKRTQARVSSKEVLKKISNCSKIHLSTKLL
                     AVDFPAHFVKSISCQICEHILADPVETSCKHLFCRICILRCLKVMGSYCPSCRYPCFP
                     TDLESPVKSFLNILNSLMVKCPAQDCNEEVSLEKYNHHVSSHKESKETLVHINKGGRP
                     RQHLLSLTRRAQKHRLRELKIQVKEFADKEEGGDVKAVCLTLFLLALRARNEHRQADE
                     LEAIMQGRGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEK
                     VLLPGYHPFEWQPPLKNVSSRTDVGIIDGLSGLASSVDEYPVDTIAKRFRYDSALVSA
                     LMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPAVPEKAVRFSF
                     TVMRITIEHGSQNVKVFEEPKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAM
                     KSSELTLEMGGIPRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDTTRLEASQN
                     LVFHSITRSHAENLQRYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDI
                     GNAAEFYKIFQLEIGEVYKHPNASKEERKRWQATLDKHLRKRMNLKPIMRMNGNFARK
                     LMTQETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQ
                     RFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFR
                     KMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKSSGFTMNSKETLGDPLGIED
                     SLESQDSMEF"
     regulatory      6647..6652
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rag1"
                     /gene_synonym="Rag-1"
                     /note="hexamer: AATAAA"
     polyA_site      6664
                     /gene="Rag1"
                     /gene_synonym="Rag-1"
                     /note="major polyA site"
ORIGIN      
        1 aagagagcaa ggagagagta gacagcatat gttgctatct ctgtggcatc gagtgttaac
       61 aaccaagctg cagacattct agcactctgg ccgggaggcc tgtggagcaa ggtagcttag
      121 ccaacatggc tgcctccttg ccgtctaccc tgagcttcag ttctgcaccc gatgaaattc
      181 aacacccaca aatcaaattt tccgagtgga aatttaagct gtttagggtg agatcctttg
      241 aaaaggcacc cgaagaagca cagaaggaga aggattcctc agaggggaaa ccttacctag
      301 aacagtctcc agtagttcca gagaagcctg gtggtcagaa ctcaattctg actcaacgag
      361 cactgaaact ccatcctaaa ttttcaaaga aattccatgc tgatgggaag tcaagcgaca
      421 aagcagttca ccaagccagg cttagacact tctgccgcat ctgtgggaat cgtttcaaga
      481 gtgacgggca cagccggaga tacccagtcc acgggcccgt ggacgctaaa acccaaagtc
      541 ttttccgaaa gaaggaaaaa agagtcactt cctggccaga cctcattgcc aggattttcc
      601 ggatcgacgt gaaggcagat gttgactcca tccacccgac ggaattctgc catgactgtt
      661 ggagcatcat gcacagaaag ttcagcagtt cccacagtca ggtctacttc ccaaggaaag
      721 tgaccgtgga gtggcacccc cacacaccgt cctgtgacat ctgttttact gcccatcggg
      781 gactcaagag gaagagacat cagcccaatg tgcagctcag caagaaacta aaaactgtgc
      841 tcaaccacgc gagacgggac cgtcgcaaga gaactcaggc tagggtcagc agcaaggaag
      901 tcctgaagaa gatctccaac tgcagtaaga ttcatctcag taccaagctt cttgccgtgg
      961 acttcccagc acactttgtg aaatccatct cctgccagat atgcgaacac attctggctg
     1021 atcccgtgga gaccagctgc aagcatctat tctgtaggat ctgcattctc agatgtctca
     1081 aagtcatggg cagctattgt ccctcttgcc gatatccgtg cttccctact gacctggaga
     1141 gcccagtgaa gtcctttctg aacatcttga attctctcat ggtcaagtgt cccgcgcaag
     1201 attgcaatga ggaagtgagt ctggaaaaat ataaccacca tgtgtcaagc cacaaagaat
     1261 ctaaagagac tttggtgcat atcaataaag ggggacggcc tcgccagcat ctcctgtcac
     1321 tgacgagaag ggcgcagaaa catcggctga gggagctcaa gattcaagtc aaagaatttg
     1381 ctgacaaaga agaaggtgga gatgtgaagg ctgtctgctt gacattgttt ctcctggcac
     1441 tgagggcgag gaatgagcac aggcaagctg atgaattaga ggccatcatg caaggcaggg
     1501 gctccgggct tcaaccagct gtttgcttgg ccatccgtgt caataccttc ctcagctgta
     1561 gccaatacca taagatgtac aggactgtga aagctatcac tgggaggcag atttttcaac
     1621 ctttgcatgc tcttcggaat gccgagaaag tccttctgcc aggctaccat ccctttgagt
     1681 ggcagccccc actgaagaat gtgtcctcca gaactgatgt tggaattatt gatgggctgt
     1741 ctggacttgc ctcctctgtg gatgagtacc cagtagatac cattgcgaag aggttccgct
     1801 acgactctgc tttggtgtct gctttgatgg acatggaaga agacatcttg gaaggcatga
     1861 gatcccaaga tcttgatgac tacctgaatg gtcccttcac agtggtggta aaggagtctt
     1921 gcgatggaat gggggatgtg agtgagaagc acgggagtgg gcccgcagtt ccagaaaagg
     1981 ccgttcgttt ctctttcaca gtcatgagaa ttacgataga gcatggttca cagaacgtga
     2041 aggtgtttga ggaacccaag cccaattctg aactgtgttg caagccgttg tgtcttatgc
     2101 tggcagatga gtctgaccat gagaccctta ctgctattct aagccccctc attgccgaga
     2161 gggaggccat gaagagcagt gaattaacgc tggagatggg aggcatcccc aggactttta
     2221 aattcatctt caggggcact ggatacgatg aaaaacttgt ccgggaagta gaaggcttgg
     2281 aagcttctgg ctcagtctac atctgtacac tctgtgacac cacccgtttg gaagcctctc
     2341 agaatcttgt cttccactcc ataaccagaa gccacgccga gaacctgcag cgctatgagg
     2401 tctggcggtc caatccgtat catgagtccg tggaagagct ccgggaccgg gtgaaagggg
     2461 tctctgccaa acctttcatc gagacagtcc cttccataga tgcgcttcac tgtgacattg
     2521 gcaatgcagc tgaattctat aagattttcc agctggagat aggggaagtg tataaacatc
     2581 ccaatgcctc taaagaggaa aggaagagat ggcaggccac gctggacaaa catctccgga
     2641 aaaggatgaa cttaaaacca atcatgagga tgaatggcaa ctttgcccgg aagcttatga
     2701 cccaagagac tgtagacgca gtttgtgagt taattccttc tgaggagagg catgaagctc
     2761 tcagggagct catggacctt tacctgaaga tgaaacccgt gtggcgctct tcatgtcccg
     2821 ctaaagagtg tccagagtcc ctctgtcagt acagtttcaa ctcacagcgt ttcgcggaac
     2881 tcctctccac caagttcaaa tatagatacg agggcaaaat caccaattac tttcacaaaa
     2941 ccttggcaca tgtccctgaa attattgaaa gggatggctc tatcggggcc tgggcaagtg
     3001 agggaaatga atcgggtaac aagctgttta gacggtttcg gaaaatgaat gccaggcagt
     3061 ccaagtgcta tgagatggaa gatgtcctga aacatcactg gctgtatact tcaaaatacc
     3121 tccagaagtt tatgaatgct cataacgcgt taaaaagctc tgggtttacc atgaactcaa
     3181 aggagacctt aggggaccct ttgggcattg aggactctct ggaaagccaa gattcaatgg
     3241 agttttaaat aggatctcca catagaagtt ggtatttgcc aatgtgtttt cctttgggtt
     3301 gcagtgaggt cttctcctag cacctagcac attgccatgt gggtgggtct tatcacccaa
     3361 ggggtgacat gttggagtga gaggccagag gtgctgtcac ctggtcaggg atcgatagtt
     3421 gacaagatga gattgcctga gccgtttagt gaggccagaa gagcaacagg agaaatcagt
     3481 tatttggaag ctcaataact tggagcagcg gtagctgcag gggaccaggg atgcacagag
     3541 atatgtgtgt gcatgccact gtgtgccatg aaaattgaag ccaaggctgt caaaggacgg
     3601 ccaagcatgc taggaaagaa atttgtcttg ggcatggttt ttttttccac ttcaatttat
     3661 tatattttgt gctgagatct atgcagtttt tttgccatgt catatttttt ggtcatattt
     3721 tataggacta ttttgtaaat ttcacatatg ttgatttatc ctacaagatt taaaggagcc
     3781 tttgaggttt ctctaacaac cctgtacatg tgtaggacgt ggtggccttt ccaagggatc
     3841 tcagtgcatt ttctcgttgc tacatttatg cataaataaa ttaaaccaag agaggtttca
     3901 gaaaatcatc cttttccagg tttaaatggc ctcagtattg tcaccttcag tgtctaggag
     3961 gtcatctggt gtcataaatt tttcaataat gaagttagag aatgcacctg ttagctacag
     4021 ctacctatta agtataataa agtcatgctt gcttagttat ctagagccag ctctggttgt
     4081 ttatttttac tttttaatta agagtcattt atagccttag tgcttttcta tgtaatctca
     4141 aggttacaga tttttaaagt ccacattttc ttactgccca aaagcagatt taataccttg
     4201 ctaaaaacgt cagtcagatt ttctctatat gaaacatggt ttgaataagt ccaggctttc
     4261 taaagtcttc ctcttttggc aatgtgttat tcttttgatt atgatcagtt gagtttcagt
     4321 gtgtgcagat tttagtagca ggactgtgct gtgctttttc tatttgttaa gtttagatgg
     4381 ggtctactca taacagagta gaggaatgaa catgtgtaca ttgtttattt atattttatt
     4441 atttttggat ttttttggat tatccccaaa gttacaggga taatcaatac ccgatttttg
     4501 aatctgaacc caagccttag tttcttgatt ctgggttctt tatactgtgt ttctgttgtc
     4561 tatacagtag cagcagaaca taattgggat ctacactaaa cactacagtt tataatccca
     4621 agagctatgt ctttggggat ggaaagtcat ctgtttgtct tgggcctttg gatagaataa
     4681 aattgggtca aacctccaag gaatccataa accctctagt ggctgcagtt atttggaaac
     4741 tattcatgtc cttaggaatt cagtattttg ccaggcacca gcaatttaag agcagccacg
     4801 ggattttatt tgctagtgag tcataatgat atgttagtgt taattagttt tttcttcctt
     4861 tgattttatt ggtcataatt gtcagtcttc atacacagta tatcaaagag ctttgtaatt
     4921 tagttgtcaa gtgtgcaaaa atgacattat cctaaatggc acatgtttgg tgcataggca
     4981 gggattgaat ttagtgcttt ctattgaaag atgcagccat tttctttgct ttctgcatgg
     5041 agcacatcga agacattcgt aatttagttg tcaaaaacat acacacactt aagtattatc
     5101 ctaaaaaccc tctctgtttt gtcatttgca aactaatagg cttctaaacc accagtcctt
     5161 tcatcccatt taatccttac agatgtctgt gcactaataa acagctaagt tcctgccacc
     5221 ctcctactcc ctggagcatg ctggcgcttt tggtagatgc cttcccatcc actcggttag
     5281 tcagttggca gcactgtaaa tactgcccga tattaaattc cccttgctag gatggtagga
     5341 caatctcaga agcatttgac tctgggcttg atttatagca catccttccc ctgactctcc
     5401 taatcagatc acattctgat aaacctctgg agcacctggc cacaggattt cattatgtag
     5461 actatcgcct atggctattt gtttccctca gtgtggagac tgcacacttg accctggtct
     5521 aggatattct acttttattt ttaagactcc ccattgcaaa tgcaggcaca tactttgggg
     5581 gaaattcacc acaaggaatg gaggattcta tcacccccac tcccccaccc caccccctgc
     5641 cccagcaggg ctggagaagt acaatgcatt tttttctgct ctgaaactgg aagcctgttt
     5701 ctacaggtct acgtgccagc cttctgccgc gcactgtggg gaccagtgac tgtagctgat
     5761 gaaaattgat gaatggtggg tgagaggtta agaagtgtct tgggattaaa agcatgcaca
     5821 cagatgccag agcagtgggg aaagatgtct ttctctctca ggtggacagg aatttaattt
     5881 tgtctcaatt tgttttcaca aagtatgcat gaaaataaag attttggggg ggggagtggg
     5941 gttgaaagta tgggtttcat atgatttcac acagatttca aattctacac agttaggagg
     6001 caggattgga ggactaagcc agggtcttgc acatgctagg caagtgtata cctctgagga
     6061 atattccaag cttcctttaa gtagtttttg agctaaggtc tcactatttt cacaggctga
     6121 ccttgaattt gagactctcc aatgccagcc tttgggtaac tgggatgaca tatgtgtgcc
     6181 cacccacggg attagttttg caagcagttg tttgatacat gtgtatgcca ttagatactg
     6241 tatggcagtt gttctagttt tcctataaga tagtttaaat cagagttgat actttgtaga
     6301 catttaaaat ttttttcttt ctttaagtat ctatgtaagc aaaacccgga attaatttgt
     6361 tttgggagat attttgtggg gttgtatttt actttattat tattgttatt attgttatta
     6421 ttttacatat attgaatgat acattttgtt caatggcttc cacgagtcta cttttcctct
     6481 ctgaggggag gtttagacac caggatgcaa tgtactattt gatttgagga cctatatttc
     6541 catgttaaat gttttcaaat ggaagaacat gagttcaaat acttattttc atattttact
     6601 ttttagcatt atgattgatt tcacaatttt gtgtttaagt atttggaata aaagtttaag
     6661 atctgaaaa
//
