LOCUS       NM_001382853            2346 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus RAD9 checkpoint clamp component B (Rad9b), transcript
            variant 2, mRNA.
ACCESSION   NM_001382853 XM_006530307
VERSION     NM_001382853.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Scekic-Zahirovic,J., Sendscheid,O., El Oussini,H., Jambeau,M.,
            Sun,Y., Mersmann,S., Wagner,M., Dieterle,S., Sinniger,J.,
            Dirrig-Grosch,S., Drenner,K., Birling,M.C., Qiu,J., Zhou,Y., Li,H.,
            Fu,X.D., Rouaux,C., Shelkovnikova,T., Witting,A., Ludolph,A.C.,
            Kiefer,F., Storkebaum,E., Lagier-Tourenne,C. and Dupuis,L.
  TITLE     Toxic gain of function from mutant FUS protein is crucial to
            trigger cell autonomous motor neuron loss
  JOURNAL   EMBO J 35 (10), 1077-1097 (2016)
   PUBMED   26951610
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Broustas,C.G., Zhu,A. and Lieberman,H.B.
  TITLE     Rad9 protein contributes to prostate tumor progression by promoting
            cell migration and anoikis resistance
  JOURNAL   J Biol Chem 287 (49), 41324-41333 (2012)
   PUBMED   23066031
REFERENCE   3  (bases 1 to 2346)
  AUTHORS   Leloup,C., Hopkins,K.M., Wang,X., Zhu,A., Wolgemuth,D.J. and
            Lieberman,H.B.
  TITLE     Mouse Rad9b is essential for embryonic development and promotes
            resistance to DNA damage
  JOURNAL   Dev Dyn 239 (11), 2837-2850 (2010)
   PUBMED   20842695
  REMARK    GeneRIF: Fndings thus indicate that Mrad9b is essential for
            embryonic development and mediates resistance to certain DNA
            damaging agents.
REFERENCE   4  (bases 1 to 2346)
  AUTHORS   He,W., Zhao,Y., Zhang,C., An,L., Hu,Z., Liu,Y., Han,L., Bi,L.,
            Xie,Z., Xue,P., Yang,F. and Hang,H.
  TITLE     Rad9 plays an important role in DNA mismatch repair through
            physical interaction with MLH1
  JOURNAL   Nucleic Acids Res 36 (20), 6406-6417 (2008)
   PUBMED   18842633
REFERENCE   5  (bases 1 to 2346)
  AUTHORS   Dufault,V.M., Oestreich,A.J., Vroman,B.T. and Karnitz,L.M.
  TITLE     Identification and characterization of RAD9B, a paralog of the RAD9
            checkpoint gene
  JOURNAL   Genomics 82 (6), 644-651 (2003)
   PUBMED   14611806
REFERENCE   6  (bases 1 to 2346)
  AUTHORS   Hopkins,K.M., Wang,X., Berlin,A., Hang,H., Thaker,H.M. and
            Lieberman,H.B.
  TITLE     Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint
            control genes in normal and cancerous testicular tissue
  JOURNAL   Cancer Res 63 (17), 5291-5298 (2003)
   PUBMED   14500360
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113285.9 and AC093473.6.
            
            On May 27, 2020 this sequence version replaced XM_006530307.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.131629.1,
                                           SRR7345562.1601754.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                AC113285.9         220558-220624
            68-138              AC093473.6         1258-1328
            139-288             AC093473.6         2162-2311
            289-403             AC093473.6         2499-2613
            404-503             AC093473.6         9575-9674
            504-610             AC093473.6         14034-14140
            611-717             AC093473.6         19356-19462
            718-782             AC093473.6         19546-19610
            783-902             AC093473.6         20457-20576
            903-1131            AC093473.6         22175-22403
            1132-1230           AC093473.6         27893-27991
            1231-1344           AC093473.6         28408-28521
            1345-2346           AC093473.6         29648-30649
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 62.3 cM"
     gene            1..2346
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /note="RAD9 checkpoint clamp component B"
                     /db_xref="GeneID:231724"
                     /db_xref="MGI:MGI:2385231"
     exon            1..67
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1299
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /EC_number="3.1.11.2"
                     /note="isoform 2 is encoded by transcript variant 2; cell
                     cycle checkpoint control protein RAD9B homolog; mRAD9B;
                     DNA repair exonuclease rad9 homolog B; RAD9 homolog B"
                     /codon_start=1
                     /product="cell cycle checkpoint control protein RAD9B
                     isoform 2"
                     /protein_id="NP_001369782.1"
                     /db_xref="GeneID:231724"
                     /db_xref="MGI:MGI:2385231"
                     /translation="MLKCGMTGGQVKVFGKAVQTLSRVSDELWLDPSEKGLALRSVNS
                     CHSTYGYVLFSSMFFQHYQWSPFATMSDTDLPLNLNCKLAIKSVLPIFRCLNYLERSV
                     EKCTVVARADKCRVVIQFFGKHGIKRTHNVYFQDSQPLKIIFEKSLCANILMIKPRLL
                     TEAIALLTSNQEEVTFSVTPGNFCLKSLSGELLDLTSSVYSEMSFGPEEFDFFQVGLD
                     TEITFCFKELKGILTFSEVMHAPLAIYFDFPGKPVVLSVEDMLLEANFILATLVDYPS
                     RTSSPQLLPLSQARRSHPIQSSAPEDKSRVSQTPESISRAAPKRLFPKDSPDSSSAAE
                     TKRASAGQDDIFEVPESVVSDMEEERSPSHLRKFSCMFFGAVSCEQQEYAGHPLDSLA
                     VASDSEEDPSAALVPVRSAVGGRKHAVKSV"
     exon            68..138
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            139..288
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            289..403
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            404..503
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            504..610
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            611..717
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            718..782
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            783..902
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1131
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1230
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1344
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..2346
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2159..2164
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2180
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /note="major polyA site"
     regulatory      2321..2326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /note="hexamer: TATAAA"
     polyA_site      2346
                     /gene="Rad9b"
                     /gene_synonym="A630082N15Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcgccctcca tgcctgctct gcccgtagcc atgctgaagt gcgggatgac gggcggccag
       61 gtgaaagtat ttgggaaagc agtacaaacg ctatcacgag tgagcgatga gctgtggcta
      121 gacccatccg agaaaggtct tgctctgaga tctgtgaatt cttgtcattc cacgtatggc
      181 tacgtcctgt tttcatctat gttttttcaa cattatcagt ggtcaccctt tgccacgatg
      241 agtgatactg acttaccact gaatttaaat tgcaaattgg caataaagtc agttctgcca
      301 atctttagat gtctgaatta ccttgaaaga agtgtggaga agtgcacagt agtcgccaga
      361 gcggataaat gcagagtcgt cattcagttc ttcggcaagc atggtattaa aagaactcac
      421 aatgtgtatt tccaagacag ccagcccttg aaaattatct tcgaaaagag tctgtgtgcg
      481 aatatcctga tgattaaacc aagactgctg actgaggcca ttgctcttct gacatcaaat
      541 caagaggaag tcaccttctc tgttactcca gggaactttt gcctcaagag tttgagtggg
      601 gaattgctgg atttgaccag ctctgtgtac agtgagatgt cttttggccc ggaagagttt
      661 gacttctttc aggttggact tgataccgag ataacatttt gctttaaaga actgaagggg
      721 atcctcacct tctcagaagt gatgcacgca cctctagcca tctactttga ctttcctgga
      781 aaaccggttg ttttgagtgt tgaagacatg ctcttggaag caaactttat cttggccacg
      841 ttagtggatt atccgagtag aacatcttca ccacagttac tgcccctttc ccaggcacga
      901 aggtcacacc ccatacagtc cagtgccccg gaggataaaa gcagagtcag tcagacccca
      961 gagagcatct ctcgagcagc gcccaaaaga ctatttccca aggattctcc tgacagcagc
     1021 tctgcagctg aaaccaagag agccagcgcc ggccaggatg acatcttcga agtgcctgaa
     1081 agtgttgtta gtgacatgga ggaagagcgg agcccttcac acctcaggaa gttttcctgc
     1141 atgttctttg gagctgtatc ttgtgaacag caagagtatg ccggccaccc cttggacagc
     1201 ctggcagtag caagcgacag tgaggaggac ccctcagctg ctctggttcc tgtgcgctct
     1261 gcagtgggag gcagaaagca tgcagtgaag tcggtgtgag tcatctgcac acagcagcca
     1321 gagctcagcc tggagaggaa caagaactat tcctgcataa gttatatttc caacatcgat
     1381 tcgggtcatt gcctctggta ggacgcacga tgatagtctc aacagaaaag gcagcgcatc
     1441 agtacacaca gactcagact tgcttcgtca tttactcatc gttgccacag actacctact
     1501 aacagttatg ttttgacatg gacactgctt tattgagaag ttcaattttg tgataaattt
     1561 acttttttga aagaaataga atccaaatgt cttctgtttc atattatgtt gttttatgtt
     1621 tttcttttct tttttttttt tttttttttt ttgctattgc aaattttttt aaatgtaaaa
     1681 ctgtgacaat ttccaagaaa gtgagtgtta caactttgcc aaagagagct gctgtacagt
     1741 gacacagagc catgaaccta ctcagacacg cacagcttca gccaatctcg ccaatcccgc
     1801 cagccgcctc caaaccactc ctgccctgtt tccatctgct ggctactcta tccagaacaa
     1861 aggcagcggc agcggcagcg gtggagggca ctctcctgcc ttggagctta tacccagtct
     1921 ctccagaccc agcccaaccc agacacatac ctggaatgaa ttctctgtga gtgagtagcc
     1981 aagcacacag ataactcggg atacatgact tgttcccctc acttctctct gtttgtaaga
     2041 ctctagctac ttcatctcca cttgataatt ttatactttt tgtgcgcagg cagcttaagt
     2101 tcataggaag aaataaggtt ctgatatgtt tagagtgcac atttttaaaa cagaacaaaa
     2161 taaaaatttc aagcatgtga taaaaatagt gattttttta taatacagta ttgctttata
     2221 aatatagatg gaaaagctat aaactttact ggtctttggt gcatccagag ggataaataa
     2281 tttgccattt gtaaattaaa acccaaattt tctcttttgt tataaaagtt cactctgtga
     2341 gacaaa
//
