LOCUS       NM_021385               2560 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus RAD18 E3 ubiquitin protein ligase (Rad18), transcript
            variant 2, mRNA.
ACCESSION   NM_021385
VERSION     NM_021385.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Kalweit,K., Golling,V., Kosan,C. and Jungnickel,B.
  TITLE     Role of Rad18 in B cell activation and lymphomagenesis
  JOURNAL   Sci Rep 14 (1), 7066 (2024)
   PUBMED   38528023
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2560)
  AUTHORS   Dickinson,K., Hammond,L., Akpa,M., Chu,L.L., Lalonde,C.T.,
            Goumba,A. and Goodyer,P.
  TITLE     WT1 regulates expression of DNA repair gene Neil3 during
            nephrogenesis
  JOURNAL   Am J Physiol Renal Physiol 324 (3), F245-F255 (2023)
   PUBMED   36546838
REFERENCE   4  (bases 1 to 2560)
  AUTHORS   Du,M., Gu,J., Liu,C., Liu,N., Yu,Z., Zhou,C., Heng,W., Cao,Z.,
            Wei,F., Zhu,K., Wang,Y., Zhang,W., Xue,X., Zhang,Y. and Qian,J.
  TITLE     Genome-wide CRISPR screen identified Rad18 as a determinant of
            doxorubicin sensitivity in osteosarcoma
  JOURNAL   J Exp Clin Cancer Res 41 (1), 154 (2022)
   PUBMED   35459258
  REMARK    GeneRIF: Genome-wide CRISPR screen identified Rad18 as a
            determinant of doxorubicin sensitivity in osteosarcoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2560)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2560)
  AUTHORS   Baarends,W.M., Wassenaar,E., van der Laan,R., Hoogerbrugge,J.,
            Sleddens-Linkels,E., Hoeijmakers,J.H., de Boer,P. and
            Grootegoed,J.A.
  TITLE     Silencing of unpaired chromatin and histone H2A ubiquitination in
            mammalian meiosis
  JOURNAL   Mol Cell Biol 25 (3), 1041-1053 (2005)
   PUBMED   15657431
REFERENCE   7  (bases 1 to 2560)
  AUTHORS   van der Laan,R., Uringa,E.J., Wassenaar,E., Hoogerbrugge,J.W.,
            Sleddens,E., Odijk,H., Roest,H.P., de Boer,P., Hoeijmakers,J.H.,
            Grootegoed,J.A. and Baarends,W.M.
  TITLE     Ubiquitin ligase Rad18Sc localizes to the XY body and to other
            chromosomal regions that are unpaired and transcriptionally
            silenced during male meiotic prophase
  JOURNAL   J Cell Sci 117 (Pt 21), 5023-5033 (2004)
   PUBMED   15383616
  REMARK    GeneRIF: Rad18Sc localizes to the XY body and to other chromosomal
            regions that are unpaired and transcriptionally silenced during
            male meiotic prophase
REFERENCE   8  (bases 1 to 2560)
  AUTHORS   Tateishi,S., Niwa,H., Miyazaki,J., Fujimoto,S., Inoue,H. and
            Yamaizumi,M.
  TITLE     Enhanced genomic instability and defective postreplication repair
            in RAD18 knockout mouse embryonic stem cells
  JOURNAL   Mol Cell Biol 23 (2), 474-481 (2003)
   PUBMED   12509447
  REMARK    GeneRIF: RAD18 contributes to maintenance of genomic stability
            through postreplication repair
REFERENCE   9  (bases 1 to 2560)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 2560)
  AUTHORS   van der Laan,R., Roest,H.P., Hoogerbrugge,J.W., Smit,E.M.,
            Slater,R., Baarends,W.M., Hoeijmakers,J.H. and Grootegoed,J.A.
  TITLE     Characterization of mRAD18Sc, a mouse homolog of the yeast
            postreplication repair gene RAD18
  JOURNAL   Genomics 69 (1), 86-94 (2000)
   PUBMED   11013078
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK012795.1 and AC157095.9.
            
            On Nov 13, 2009 this sequence version replaced NM_021385.1.
            
            Transcript Variant: This variant (2) lacks two alternate in-frame
            exons compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK012795.1, AF205278.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2526              AK012795.1         2-2527
            2527-2560           AC157095.9         47123-47156
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 52.42 cM"
     gene            1..2560
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /note="RAD18 E3 ubiquitin protein ligase"
                     /db_xref="GeneID:58186"
                     /db_xref="MGI:MGI:1890476"
     exon            1..127
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..1606
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2;
                     post-replication repair protein RAD18SC; E3
                     ubiquitin-protein ligase RAD18; mRAD18Sc; postreplication
                     repair protein RAD18; RAD18 homolog; RING-type E3
                     ubiquitin transferase RAD18"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RAD18 isoform 2"
                     /protein_id="NP_067360.2"
                     /db_xref="CCDS:CCDS39589.1"
                     /db_xref="GeneID:58186"
                     /db_xref="MGI:MGI:1890476"
                     /translation="MEVLAEPRWPPGLAVMKTIDDLLRCGICFEYFNIAVIIPQCSHN
                     YCSLCIRKFLSYKTQCPTCCVAVTEPDLRNNRLLDELVKSMNFARTHLLQFALESPPI
                     SPVSSTSKKVVVKVHNADAAQHPVKQANRLMDKFLIRETGDCVFELLGKENERKFSPQ
                     KELSTSAEIKETSLLGKPVLGLSDANGPVTPSTSTMKLDTKVSCPVCGVSIPENHINK
                     HLDSCLSREEKKESLRSSAHKRKPLPKTVYNLLSDRDLKKKLKQYGLSVQGNKQQLIK
                     RHQEFVHMYNAQCDALHPKSAAEIVQEIESMEKTRMRLEASKLNENVMVFTKNQTEKE
                     IEEVHSEYRKKHQNAFQLLVDQAKKGYKKTGRVSQAAAMRTDEPAETLPSMRTDEPAE
                     TLPSMRTDEPAETLPLMRADEPAETLPSECIAQEDNVSFSDTVSVTNHFPQPQLDSPG
                     PSEPERPDDSSSCTDILFSSDSDSCNRNDQNREVSPQQTRRTRASECVEIEPRNKRNK
                     N"
     exon            128..209
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            210..271
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            272..342
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            343..680
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            681..780
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            781..965
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1042
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1103
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1346
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1506
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..2560
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2416..2421
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /note="hexamer: CATAAA"
     polyA_site      2438
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
     regulatory      2517..2522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /note="hexamer: CATAAA"
     polyA_site      2542
                     /gene="Rad18"
                     /gene_synonym="2810024C04Rik; Rad18sc"
                     /note="major polyA site"
ORIGIN      
        1 acggtgtagc gcggggaatt tcgagtagag gagctgcgtg gcgggccggt agctggcggt
       61 ctcatcaggt gcggccatgg aggtcctggc cgagccgcga tggcctccgg ggttggcggt
      121 gatgaagaca atagatgact tgctgcgctg tgggatttgc tttgagtatt tcaacattgc
      181 agtgataatc ccccagtgct ctcacaacta ttgttcactc tgtataagaa agtttttatc
      241 ctataaaact cagtgcccaa cttgttgcgt ggcagtaacg gagccagacc tgagaaataa
      301 tcgcctctta gatgaactgg taaaaagcat gaattttgca cggactcacc tgttgcagtt
      361 tgctttagag tcaccaccca tatctcctgt gtcttccacc tcaaagaagg ttgttgttaa
      421 agtgcataat gctgacgccg cccaacaccc tgtcaaacag gcgaacaggt taatggataa
      481 gttcctgatt agagaaactg gtgactgtgt atttgagttg ttgggaaaag aaaatgagag
      541 gaaattcagc cctcaaaaag agctaagcac ctctgctgag attaaagaga caagtctcct
      601 aggaaagccg gtactggggc tctcggatgc taatggtcct gtgactccct ctacatccac
      661 tatgaaactg gatactaaag tgtcttgtcc tgtttgtggg gtcagcattc cagaaaatca
      721 tatcaataag catttagaca gttgtttatc acgtgaagag aagaaggaga gcctgcgaag
      781 ttctgctcac aaaaggaagc cgttgcccaa aactgtatat aacttgctct ctgatcgtga
      841 tttaaagaaa aagctgaaac agtatggctt atctgttcaa ggaaacaaac agcagcttat
      901 taaaaggcat caggagtttg tgcacatgta taatgcccag tgcgatgctt tgcatcctaa
      961 atcagctgct gaaatcgtcc aagaaattga aagcatggag aagaccagga tgcgccttga
     1021 agcaagtaaa ctcaatgaaa atgtcatggt ttttacaaag aaccaaacag agaaggaaat
     1081 tgaggaagtt cacagtgaat atcgtaaaaa gcaccagaat gcattccagc ttctggtgga
     1141 tcaggccaaa aaagggtata agaaaactgg cagagtttca caagctgcag cgatgagaac
     1201 agatgaacct gcagagacac tgccgtcgat gagaacagat gaacctgcag agacactgcc
     1261 gtcgatgaga acagatgaac ctgcagagac actgccgttg atgagagcag atgaacctgc
     1321 agaaacactg ccgtctgagt gtatcgcaca agaagataat gtgagcttct cagatactgt
     1381 ctcagtaaca aaccactttc ctcagcccca gctggactcg ccaggcccgt cggagcctga
     1441 gaggccggat gattcttcta gttgtactga tattcttttc tcctcggact cagactcatg
     1501 caatagaaat gatcaaaaca gagaagtcag cccacaacag actcgccgca ctagagccag
     1561 tgaatgtgtt gagattgaac caagaaacaa gcggaataag aattagtatg ggctttgtgc
     1621 cagctttccg tacagtgacc tgaatatgat attgttattg ccatgtatgg cttcatattt
     1681 aacaatatcc aaggaagact tttaagtcta aatgtaatag ttaatttatt ttcatgcttc
     1741 tttgccttcc tgaacaacaa caaaacaaac acacacacac acacacacac aaacaaaaac
     1801 caaaaacaaa caaacaaaaa aacgtgaaat gttctgaaat gactcttctt agtcagttgg
     1861 ttccttcctt tcctttgaga catttctccc ctaaaatcat actcaccagc tttgcacaga
     1921 gtatacattt cttagcggga gcgttttcct tggtttgact tctctatttc aggatgtttt
     1981 taagtcattt tttatttttt ccactgggga ccaaacctca gaagcagctt ttcatcctct
     2041 tgacagttgc aagcctgccg ctgagagtgt gcatgctctt gtcctaacca cacacccttg
     2101 gtgggagcgc ctgctgccag gccaccgcag ttactgctta gtacccaagt tgactgtgtt
     2161 tgttagacac acctgtgttt acttatagac taggtttata atggggcggg gtaggggtca
     2221 ttttatggtg tttgaaagct ggggaagtag ggatgtcagt gctgtgcttt aagggcgcag
     2281 tctcaatgac tgaagcatat gtatgtcctt ctgaagtggc acttaaagtt atcactctta
     2341 gtgttcttcc actggggtca ggcagatctc taacactttg aatcccagct gttaaacaaa
     2401 caaacaaaaa agtgacataa atgttattta tcaatgaata tgcaatgtgt aatgtctttg
     2461 aaacagtggc ttgtgagtat ttgaatagag cctacaaaat tttatattgc atttgtcata
     2521 aaatttgttt ctgtgtgaaa aatgccttta tttcccagtt
//
