LOCUS       NM_011232               1479 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript
            variant 1, mRNA.
ACCESSION   NM_011232
VERSION     NM_011232.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Kasowitz,S.D., Ma,J., Anderson,S.J., Leu,N.A., Xu,Y., Gregory,B.D.,
            Schultz,R.M. and Wang,P.J.
  TITLE     Nuclear m6A reader YTHDC1 regulates alternative polyadenylation and
            splicing during mouse oocyte development
  JOURNAL   PLoS Genet 14 (5), e1007412 (2018)
   PUBMED   29799838
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1479)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 1479)
  AUTHORS   Lyndaker,A.M., Lim,P.X., Mleczko,J.M., Diggins,C.E., Holloway,J.K.,
            Holmes,R.J., Kan,R., Schlafer,D.H., Freire,R., Cohen,P.E. and
            Weiss,R.S.
  TITLE     Conditional inactivation of the DNA damage response gene Hus1 in
            mouse testis reveals separable roles for components of the
            RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance
  JOURNAL   PLoS Genet 9 (2), e1003320 (2013)
   PUBMED   23468651
  REMARK    GeneRIF: HUS1 acts as a component of the canonical 9-1-1 complex
            during meiotic prophase I to promote DSB repair and further propose
            that RAD1 and TOPBP1 respond to unsynapsed chromatin through an
            alternative mechanism that does not require RAD9 or HUS1.
REFERENCE   5  (bases 1 to 1479)
  AUTHORS   Refolio,E., Cavero,S., Marcon,E., Freire,R. and San-Segundo,P.A.
  TITLE     The Ddc2/ATRIP checkpoint protein monitors meiotic recombination
            intermediates
  JOURNAL   J Cell Sci 124 (Pt 14), 2488-2500 (2011)
   PUBMED   21693576
REFERENCE   6  (bases 1 to 1479)
  AUTHORS   Dean,F.B., Lian,L. and O'Donnell,M.
  TITLE     cDNA cloning and gene mapping of human homologs for
            Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of
            homologs from mouse, Caenorhabditis elegans, and Drosophila
            melanogaster
  JOURNAL   Genomics 54 (3), 424-436 (1998)
   PUBMED   9878245
REFERENCE   7  (bases 1 to 1479)
  AUTHORS   Bluyssen,H.A., van Os,R.I., Naus,N.C., Jaspers,I., Hoeijmakers,J.H.
            and de Klein,A.
  TITLE     A human and mouse homolog of the Schizosaccharomyces pombe rad1+
            cell cycle checkpoint control gene
  JOURNAL   Genomics 54 (2), 331-337 (1998)
   PUBMED   9828137
REFERENCE   8  (bases 1 to 1479)
  AUTHORS   Udell,C.M., Lee,S.K. and Davey,S.
  TITLE     HRAD1 and MRAD1 encode mammalian homologues of the fission yeast
            rad1(+) cell cycle checkpoint control gene
  JOURNAL   Nucleic Acids Res 26 (17), 3971-3976 (1998)
   PUBMED   9705507
REFERENCE   9  (bases 1 to 1479)
  AUTHORS   Freire,R., Murguia,J.R., Tarsounas,M., Lowndes,N.F., Moens,P.B. and
            Jackson,S.P.
  TITLE     Human and mouse homologs of Schizosaccharomyces pombe rad1(+) and
            Saccharomyces cerevisiae RAD17: linkage to checkpoint control and
            mammalian meiosis
  JOURNAL   Genes Dev 12 (16), 2560-2573 (1998)
   PUBMED   9716408
REFERENCE   10 (bases 1 to 1479)
  AUTHORS   Parker,A.E., Van de Weyer,I., Laus,M.C., Oostveen,I., Yon,J.,
            Verhasselt,P. and Luyten,W.H.
  TITLE     A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint
            gene encodes an exonuclease
  JOURNAL   J Biol Chem 273 (29), 18332-18339 (1998)
   PUBMED   9660799
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102101.7.
            
            On Aug 31, 2022 this sequence version replaced NM_011232.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146533.1, AK148098.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               AC102101.7         37020-37235         c
            217-480             AC102101.7         36568-36831         c
            481-589             AC102101.7         35186-35294         c
            590-848             AC102101.7         32770-33028         c
            849-947             AC102101.7         30421-30519         c
            948-1479            AC102101.7         29599-30130         c
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 5.35 cM"
     gene            1..1479
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /note="RAD1 checkpoint DNA exonuclease"
                     /db_xref="GeneID:19355"
                     /db_xref="MGI:MGI:1316678"
     exon            1..216
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            217..480
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             283..1125
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /EC_number="3.1.11.2"
                     /note="DNA repair exonuclease rad1 homolog; rad1-like DNA
                     damage checkpoint protein; RAD1 homolog"
                     /codon_start=1
                     /product="cell cycle checkpoint protein RAD1"
                     /protein_id="NP_035362.2"
                     /db_xref="CCDS:CCDS27379.1"
                     /db_xref="GeneID:19355"
                     /db_xref="MGI:MGI:1316678"
                     /translation="MPLLTQYNEEEYEQYCLVASLDNVRNLSTVLKAIHFREHATCFA
                     TKNGIKVTVENAKCVQANAFIQADVFQEFVIQEESVTFRINLTILLDCLSIFGSSPTP
                     GTLTALRMCYQGYGHPLMLFLEEGGVVTVCKITTQEPEETLDFDFCSTNVMNKIILQS
                     EGLREAFSELDMTGDVLQITVSPDKPYFRLSTFGNAGNSHLDYPKDSDLVEAFHCDKT
                     QVNRYKLSLLKPSTKALALSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEE
                     VPES"
     exon            481..589
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            590..848
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            849..947
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1479
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1447..1452
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /note="hexamer: AATAAA"
     polyA_site      1479
                     /gene="Rad1"
                     /gene_synonym="mRAD1"
                     /note="major polyA site"
ORIGIN      
        1 ggctcggtgg tgacggtggc ctaggtggtt gcgtatctga acggtggcgg gagctgtttg
       61 cctggcttgc tagctttttg gacgctcagg gtgttcttgg ggctggggtg gcaggggctt
      121 tgaggggtcg gacgtcaccc acagcctggc gccaggggct cccttagtgg ccgctgtgcg
      181 gcgagcgggc cgaagtcgcg ttctcctgca agccaggttt tcacacatct tccggaagag
      241 gagcatcctt cttctcggag ccgacggtac cgtggaccat ccatgcctct cctaacccag
      301 tacaatgaag aggagtacga acagtactgc ttagtggcca gccttgacaa cgttaggaat
      361 ctctccactg tcttgaaagc cattcatttc agagaacacg ccacgtgttt tgctaccaaa
      421 aacggaatca aggttacagt ggagaatgca aagtgtgtgc aagcaaatgc ctttattcag
      481 gctgacgtgt ttcaggaatt tgtcattcag gaagaatctg ttacttttcg aattaactta
      541 actatccttt tagactgttt atctattttt ggatcaagtc ctacaccagg gactttgact
      601 gcgcttcgga tgtgttacca aggttatggt cacccactga tgctatttct agaagaagga
      661 ggagtggtga cggtctgcaa aattaccact caggagcctg aggagacact ggattttgat
      721 ttctgcagca ccaatgttat gaataaaatt atcctgcagt cagaggggct ccgggaagcc
      781 ttttctgagc tggacatgac aggtgatgtc ctacagatca ctgtgtctcc tgacaagccc
      841 tatttcaggt tgtctacttt tggaaatgca ggaaactccc atcttgacta tcccaaagat
      901 tccgacttgg tggaagcctt tcactgtgat aagacccagg tcaacagata caagctgtcg
      961 ctactgaagc cctctacaaa ggcactagct ttatcctgta aagtgtctat ccggacagat
     1021 aaccgaggct tcctctcctt acagtacatg attagaaatg aagatgggca gatatgtttt
     1081 gtggaatatt actgctgccc tgatgaagaa gttcctgagt cttgaataat tcactgacta
     1141 tttctgtctt tttttttttt tctttcacga gacagggttt ctctgtagcc ctggctgtcc
     1201 tggaactcac tttgtagacc aggctggcct cgaactcaga aattcacctg cctctgcctc
     1261 ccgagtgctg ggattaaagg catgcgccac cacgaccggc gaatatttgt gtctttatag
     1321 ttggcgttct cactctaact ctctaacctt gatgatttca ttttggattg tatagagaga
     1381 tgcatggaga aaataggagc aaggtcagtc ccattctgta aatctgtaaa tagtttttat
     1441 tttgtcaata aatttttcat atagccataa attatccga
//
