LOCUS       NM_023126               2012 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus RAB8A, member RAS oncogene family (Rab8a), mRNA.
ACCESSION   NM_023126 XM_981797
VERSION     NM_023126.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Singh,S., Tehseen,A., Dahiya,S., Singh,Y.J., Sarkar,R. and
            Sehrawat,S.
  TITLE     Rab8a restores diverse innate functions in CD11c+CD11b+ dendritic
            cells from aged mice
  JOURNAL   Nat Commun 15 (1), 10300 (2024)
   PUBMED   39604443
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2012)
  AUTHORS   Wu,J., Moriwaki,K., Asuka,T., Nakai,R., Kanda,S., Taniguchi,M.,
            Sugiyama,T., Yoshimura,S.I., Kunii,M., Nagasawa,T., Hosen,N.,
            Miyoshi,E. and Harada,A.
  TITLE     EHBP1L1, an apicobasal polarity regulator, is critical for nuclear
            polarization during enucleation of erythroblasts
  JOURNAL   Blood Adv 7 (14), 3382-3394 (2023)
   PUBMED   37042948
  REMARK    Erratum:[Blood Adv. 2024 Mar 12;8(5):1151. doi:
            10.1182/bloodadvances.2023011838.. PMID: 38421726]
REFERENCE   4  (bases 1 to 2012)
  AUTHORS   Ouyang,Q., Chen,Q., Ke,S., Ding,L., Yang,X., Rong,P., Feng,W.,
            Cao,Y., Wang,Q., Li,M., Su,S., Wei,W., Liu,M., Liu,J., Zhang,X.,
            Li,J.Z., Wang,H.Y. and Chen,S.
  TITLE     Rab8a as a mitochondrial receptor for lipid droplets in skeletal
            muscle
  JOURNAL   Dev Cell 58 (4), 289-305 (2023)
   PUBMED   36800997
REFERENCE   5  (bases 1 to 2012)
  AUTHORS   Liu,Y., Zhang,Z., Gao,X., Ma,Q., Yu,Z. and Huang,S.
  TITLE     Rab8A promotes breast cancer progression by increasing surface
            expression of Tropomyosin-related kinase B
  JOURNAL   Cancer Lett 535, 215629 (2022)
   PUBMED   35278612
  REMARK    GeneRIF: Rab8A promotes breast cancer progression by increasing
            surface expression of Tropomyosin-related kinase B.
            Erratum:[Cancer Lett. 2022 Jul 28;539:215701. doi:
            10.1016/j.canlet.2022.215701.. PMID: 35527117]
REFERENCE   6  (bases 1 to 2012)
  AUTHORS   Ren,M., Zeng,J., De Lemos-Chiarandini,C., Rosenfeld,M., Adesnik,M.
            and Sabatini,D.D.
  TITLE     In its active form, the GTP-binding protein rab8 interacts with a
            stress-activated protein kinase
  JOURNAL   Proc Natl Acad Sci U S A 93 (10), 5151-5155 (1996)
   PUBMED   8643544
REFERENCE   7  (bases 1 to 2012)
  AUTHORS   Rao,G., Alland,L., Guida,P., Schreiber-Agus,N., Chen,K., Chin,L.,
            Rochelle,J.M., Seldin,M.F., Skoultchi,A.I. and DePinho,R.A.
  TITLE     Mouse Sin3A interacts with and can functionally substitute for the
            amino-terminal repression of the Myc antagonist Mxi1
  JOURNAL   Oncogene 12 (5), 1165-1172 (1996)
   PUBMED   8649810
REFERENCE   8  (bases 1 to 2012)
  AUTHORS   Nimmo,E.R., Sanders,P.G., Padua,R.A., Hughes,D., Williamson,R. and
            Johnson,K.J.
  TITLE     The MEL gene: a new member of the RAB/YPT class of RAS-related
            genes
  JOURNAL   Oncogene 6 (8), 1347-1351 (1991)
   PUBMED   1886711
REFERENCE   9  (bases 1 to 2012)
  AUTHORS   Howard,T.A., Rochelle,J.M., Saunders,A.M. and Seldin,M.F.
  TITLE     A linkage map of mouse chromosome 8: further definition of
            homologous linkage relationships between mouse chromosome 8 and
            human chromosomes 8, 16, and 19
  JOURNAL   Genomics 10 (1), 207-213 (1991)
   PUBMED   1675193
REFERENCE   10 (bases 1 to 2012)
  AUTHORS   Saunders,A.M. and Seldin,M.F.
  TITLE     A molecular genetic linkage map of mouse chromosome 7
  JOURNAL   Genomics 8 (3), 525-535 (1990)
   PUBMED   1981054
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY279480.1, AK010862.1, AK009259.1, AK076048.1 and BY700504.1.
            
            On or before May 4, 2006 this sequence version replaced
            XM_981797.1, NM_023126.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076048.1, AK079306.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-174               BY279480.1         2-175
            175-810             AK010862.1         26-661
            811-1484            AK009259.1         662-1335
            1485-2006           AK076048.1         1364-1885
            2007-2012           BY700504.1         389-394
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.84 cM"
     gene            1..2012
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /note="RAB8A, member RAS oncogene family"
                     /db_xref="GeneID:17274"
                     /db_xref="MGI:MGI:96960"
     exon            1..283
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..783
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /EC_number="3.6.5.2"
                     /note="cell line NK14 derived transforming oncogene;
                     oncogene c-mel"
                     /codon_start=1
                     /product="ras-related protein Rab-8A"
                     /protein_id="NP_075615.2"
                     /db_xref="CCDS:CCDS22409.1"
                     /db_xref="GeneID:17274"
                     /db_xref="MGI:MGI:96960"
                     /translation="MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGID
                     FKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNW
                     IRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVEN
                     AFFTLARDIKAKMDKKLEGNSPQGSSHGVKITVEQQKRTSFFRCSLL"
     exon            284..344
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /inference="alignment:Splign:2.1.0"
     exon            345..405
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /inference="alignment:Splign:2.1.0"
     exon            406..483
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /inference="alignment:Splign:2.1.0"
     exon            484..573
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /inference="alignment:Splign:2.1.0"
     exon            574..639
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /inference="alignment:Splign:2.1.0"
     exon            640..690
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /inference="alignment:Splign:2.1.0"
     exon            691..2012
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1492
                     /gene="Rab8a"
                     /gene_synonym="Mel"
     regulatory      1496..1501
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /note="hexamer: AATGAA"
     regulatory      1901..1906
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /note="hexamer: ATTAAA"
     polyA_site      1924
                     /gene="Rab8a"
                     /gene_synonym="Mel"
                     /note="major polyA site"
ORIGIN      
        1 gggctctctg cgacaggcgg ggctccgggt ggcgggcgta ggcctggatg atgcgcggcg
       61 ggcagggggc caataggggg cgggatctga gatgatagac gggtccagcg accagtggcg
      121 aggcgccggc cgtacttccc gtcggggaga gagtgtaata tggcgaagac ctacgattac
      181 ctgttcaagc tgctgctgat cggggactcg ggggtaggga agacctgtgt cctgttccgc
      241 ttctccgagg acgccttcaa ctccacattc atctctacca taggaattga ctttaaaatt
      301 aggaccatag agctcgatgg caagaggatt aaactgcaga tatgggacac ggccggccag
      361 gagcggtttc gaacaatcac gacagcctac tacaggggtg ccatgggtat catgctggtc
      421 tacgacatta ccaatgagaa gtcctttgac aacatccgga attggattcg gaacattgaa
      481 gagcatgcct ctgcagacgt ggagaagatg atactgggga ataagtgtga tgtgaatgac
      541 aagagacagg tgtccaagga acggggagaa aagctggcac tcgactatgg gatcaagttc
      601 atggagacca gtgcaaaggc caacatcaat gtggagaatg catttttcac tcttgccagg
      661 gatatcaaag caaaaatgga caaaaaattg gaagggaaca gcccgcaggg gagcagccat
      721 ggagtcaaga tcacagtgga gcagcagaag aggaccagct tcttccggtg cagtctcctg
      781 tgaggacaag gtctctgccc attctgagcc tgtgctcagc ccagctgact gtgcctgccc
      841 cgggccagcc tccacctctg atgtttggac acggctgccc ggactcgatt gagaaattga
      901 ttatttttta gtaaccgtct gatcttttcc accttcagag atggagagag ttagaatttt
      961 gctgtttttc ctatgtcatc tgccagtcag gcctatccgt cccctgcctc ataggcacag
     1021 atggagagag gttgggtggg ggcccggcag cctccatttc cagccttctg tccttccagc
     1081 acagaaggta gccagcggta gtgcccagcc tgcctggccc ccatactcca agcaccacca
     1141 ccggggccac tgagggacaa gcctgcacct gccttcagct gttctcagtt gtggacaagt
     1201 gacagccatg tccactgtct ccttacgtgt caaaccaaag ggaagcacaa actaaggaaa
     1261 gctcacttag aggattttgg aagaagtgga ccgcttccat ggccagagca ggatgggagt
     1321 ctgcgattca gactgaggtg acgtcaccac cattctccca tgacccactg tcattgggcc
     1381 acctgcctca gtggcaccaa aaccgcctcc ccaatcttct ctggctggct ctaatcggat
     1441 gttcattttc tatctaagat gattttttta tttttccaga ttcctaacac catgaaatga
     1501 atctgtcttt gattttctag cagcatacct gggaaattgg gaaccagaat gactgccctg
     1561 tgcccacagg gcacctggtc cctgagtccc agacaagcat tccatggcac agcgctcccc
     1621 tgctgtcttt acccagttgt gatgtgacag ctctgtcctc ctggggagac tgattgggac
     1681 tgatgtcctg gtgggagggt gtcctggtgg gactgatgtc ctggtgggac tgatgtcctg
     1741 gtgggagggt gtcctggtgg gactgatgtc ctggtgggac tgatgtcctg gtgggagggt
     1801 gtcctttggg agcacctttc tttttactct aactcttccc ccaagctcca agctgctgtc
     1861 agtccaagct gtcagtatca aaatctctcc cttttttgaa attaaaacca acgtaccagc
     1921 aatagtcacc tgccctcccc tggagtctct gatgtaccat gtttacactg actagtgcac
     1981 tcagaaaatt agattaaagt gcatttaaaa ag
//
