LOCUS       NM_001159516            5378 bp    mRNA    linear   ROD 14-OCT-2024
DEFINITION  Mus musculus quaking, KH domain containing RNA binding (Qki),
            transcript variant 2, mRNA.
ACCESSION   NM_001159516
VERSION     NM_001159516.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Hayakawa-Yano,Y., Furukawa,T., Matsuo,T., Ogasawara,T., Nogami,M.,
            Yokoyama,K., Yugami,M., Shinozaki,M., Nakamoto,C., Sakimura,K.,
            Koyama,A., Ogi,K., Onodera,O., Takebayashi,H., Okano,H. and Yano,M.
  TITLE     Qki5 safeguards spinal motor neuron function by defining the motor
            neuron-specific transcriptome via pre-mRNA processing
  JOURNAL   Proc Natl Acad Sci U S A 121 (37), e2401531121 (2024)
   PUBMED   39226364
  REMARK    GeneRIF: Qki5 safeguards spinal motor neuron function by defining
            the motor neuron-specific transcriptome via pre-mRNA processing.
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Montanes-Agudo,P., van der Made,I., Aufiero,S., Tijsen,A.J.,
            Pinto,Y.M. and Creemers,E.E.
  TITLE     Quaking regulates circular RNA production in cardiomyocytes
  JOURNAL   J Cell Sci 136 (13) (2023)
   PUBMED   37272356
REFERENCE   3  (bases 1 to 5378)
  AUTHORS   Yan,Z., Ruan,B., Wang,S., Du,T., Shao,X., Chen,G., Wang,L.,
            Zhai,D., Zhu,S., Lu,Z. and Cao,X.
  TITLE     RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via
            Suppressing Wnt Pathway
  JOURNAL   Arch Med Res 54 (5), 102853 (2023)
   PUBMED   37460362
  REMARK    GeneRIF: RNA-binding Protein QKI Inhibits Osteogenic
            Differentiation Via Suppressing Wnt Pathway.
REFERENCE   4  (bases 1 to 5378)
  AUTHORS   Montanes-Agudo,P., Aufiero,S., Schepers,E.N., van der Made,I.,
            Cocera-Ortega,L., Ernault,A.C., Richard,S., Kuster,D.W.D.,
            Christoffels,V.M., Pinto,Y.M. and Creemers,E.E.
  TITLE     The RNA-binding protein QKI governs a muscle-specific alternative
            splicing program that shapes the contractile function of
            cardiomyocytes
  JOURNAL   Cardiovasc Res 119 (5), 1161-1174 (2023)
   PUBMED   36627242
  REMARK    GeneRIF: The RNA-binding protein QKI governs a muscle-specific
            alternative splicing program that shapes the contractile function
            of cardiomyocytes.
REFERENCE   5  (bases 1 to 5378)
  AUTHORS   Zhai,D., Wang,W., Ye,Z., Xue,K., Chen,G., Hu,S., Yan,Z., Guo,Y.,
            Wang,F., Li,X., Xiang,A., Li,X., Lu,Z. and Wang,L.
  TITLE     QKI degradation in macrophage by RNF6 protects mice from MRSA
            infection via enhancing PI3K p110beta dependent autophagy
  JOURNAL   Cell Biosci 12 (1), 154 (2022)
   PUBMED   36088389
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5378)
  AUTHORS   Mikoshiba,K., Nagaike,K., Aoki,E. and Tsukada,Y.
  TITLE     Biochemical and immunohistochemical studies on dysmyelination of
            quaking mutant mice in vivo and in vitro
  JOURNAL   Brain Res 177 (2), 287-299 (1979)
   PUBMED   227533
REFERENCE   7  (bases 1 to 5378)
  AUTHORS   Greenfield,S., Williams,N.I., White,M., Brostoff,S.W. and
            Hogan,E.L.
  TITLE     Proteolipid protein: synthesis and assembly into quaking mouse
            myelin
  JOURNAL   J Neurochem 32 (6), 1647-1651 (1979)
   PUBMED   448358
REFERENCE   8  (bases 1 to 5378)
  AUTHORS   Fagg,G.E.
  TITLE     The quaking mouse: regional variations in the content and protein
            composition of myelin isolated from the central nervous system
  JOURNAL   Neuroscience 4 (7), 973-978 (1979)
   PUBMED   552615
REFERENCE   9  (bases 1 to 5378)
  AUTHORS   Suzuki,K. and Zagoren,J.C.
  TITLE     Quaking mouse: an ultrastructural study of the peripheral nerves
  JOURNAL   J Neurocytol 6 (1), 71-84 (1977)
   PUBMED   190360
REFERENCE   10 (bases 1 to 5378)
  AUTHORS   Friedrich,V.L. Jr.
  TITLE     Hyperplasia of oligodendrocytes in quaking mice
  JOURNAL   Anat Embryol (Berl) 147 (3), 259-271 (1975)
   PUBMED   174456
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154238.2 and AC113114.9.
            
            On Nov 25, 2022 this sequence version replaced NM_001159516.1.
            
            Transcript Variant: This variant (2) differs in the 3' UTR and
            coding sequence compared to variant 1. The resulting isoform (2)
            has a shorter and distinct C-terminus compared to isoform 1.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132303.1, SRR14777534.972378.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-574               AC154238.2         32594-33167         c
            575-717             AC113114.9         27091-27233
            718-834             AC113114.9         36009-36125
            835-978             AC113114.9         71036-71179
            979-1066            AC113114.9         92269-92356
            1067-1366           AC113114.9         93613-93912
            1367-5378           AC113114.9         95878-99889
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 7.75 cM"
     gene            1..5378
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /note="quaking, KH domain containing RNA binding"
                     /db_xref="GeneID:19317"
                     /db_xref="MGI:MGI:97837"
     exon            1..574
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /inference="alignment:Splign:2.1.0"
     CDS             433..1392
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein quaking; lethal, Chr 17, U Wisconsin 1; QKI-5
                     protein; QKI-6 protein; KH domain-containing RNA-binding
                     protein QKI; QKI-7b protein; QKI-7 protein"
                     /codon_start=1
                     /product="KH domain-containing RNA-binding protein QKI
                     isoform 2"
                     /protein_id="NP_001152988.1"
                     /db_xref="CCDS:CCDS88989.1"
                     /db_xref="GeneID:19317"
                     /db_xref="MGI:MGI:97837"
                     /translation="MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERL
                     LDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRI
                     LGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDA
                     QNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSL
                     AATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVP
                     PGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGMAFPTKG"
     exon            575..717
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /inference="alignment:Splign:2.1.0"
     exon            718..834
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /inference="alignment:Splign:2.1.0"
     exon            835..978
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1066
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1366
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..5378
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5357..5362
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /note="hexamer: AATAAA"
     polyA_site      5378
                     /gene="Qki"
                     /gene_synonym="1110003F05Rik; 1500005P18; l(17)-1Wis;
                     l17Wis1; Qk"
                     /note="major polyA site"
ORIGIN      
        1 gcagtcggag cgcggcggcg gcggcggcgg cggtaacgcg gagcgccgcg gcggacagca
       61 ccgcagactc gcgagctccg gcgcaggcgg cgcgaccggc cgacgagcgg ccccccggct
      121 cctcgcggcg cagcggcccc gcgccgcgct cggcccgaga cccgcctctg ccgccggcct
      181 cgcgagggga gccgcgccgg gcggccgagg agcggcggcc gcggcgcgtc gggccgggcg
      241 aaagtgcctg cggggggcgg gcgggcgcgc ggagccggcc gccccggggc tcggcgcggg
      301 agccggagcg ggagccggcg cggagcggga cgccgcgtcc cgagcggccg gcggccggag
      361 ctcgcccccg cccctccctc ctctcctccg gcggcggcgg cggcgggcgg agtgagctgc
      421 ggagcctgga atatggtcgg ggaaatggaa acgaaggaga agccgaagcc caccccagat
      481 tatttgatgc agctgatgaa cgacaagaag ctcatgagca gcctgcccaa cttctgcggg
      541 atcttcaacc acctcgagcg gctgctggac gaagaaatta gcagagtacg gaaagacatg
      601 tacaatgaca cgttaaatgg cagtacagag aaaagaagtg cagaattgcc tgacgcggtg
      661 ggacccattg ttcagttaca agagaaactt tatgtgcctg taaaagaata ccctgatttt
      721 aattttgttg ggagaatcct tggacctaga ggacttacag ctaaacaact tgaagcagaa
      781 acgggatgta aaataatggt ccgaggcaaa ggctcaatga gggataaaaa gaaggaggag
      841 caaaatagag gcaagcccaa ttgggagcat ctaaatgaag acttacatgt actaatcact
      901 gtggaagatg ctcagaacag agcagaaatc aagctgaaga gagcggttga agaagtgaag
      961 aagttactgg tacctgcggc tgaaggtgaa gacagcctga agaagatgca gctgatggag
     1021 cttgcaattc tgaatggcac ctacagagac gccaacatta aatcaccagc ccttgccttt
     1081 tctcttgcag caactgccca ggctgctcca aggatcatca ctgggcctgc gcctgtcctc
     1141 ccaccagctg ctctgcgtac acctacgcca gctggcccta ccataatgcc tttgatcaga
     1201 caaatacaga ccgctgtcat gccaaacgga actcctcacc caactgctgc aatagtccct
     1261 ccagggcctg aagctgggtt aatctacaca ccctatgaat acccctacac attggcacca
     1321 gctacatcaa tccttgagta ccctattgaa cccagtggtg tgttaggtat ggctttccca
     1381 acgaaaggct aagaattcaa gaacggtctt aaccgaaccc tcatcagatc tgaatttaac
     1441 aaatgcttag tctcagcagc ctccgggaaa aaccttagcc tagcagtcag tgacttactt
     1501 gcactttttg cacatagata taaagtaaaa tgatactatt aatttggatt agtctgtaat
     1561 actgcaaagc aacagtaatt tataaaggag tgtatagaag taaactgact gctaagtgta
     1621 ctgtactgtt tgctttacta atcacctaat tcattgcagt tcatacttat tgactcaaaa
     1681 gtttaaatcc acaatctgta ggctttaatt aagagttgct ttttaaacct tttttaagtg
     1741 ataaactctg aaagttaagg ttagcaaaat aattgtcagt attaaatgtg gcattattta
     1801 agtagtcctg ctgcaagaaa ggcacttcag tgaatatgtg actagtaaag ctcagacacg
     1861 tctggttcta atagagctcg tgagatactg tgacttgggg actgtcaatg aagaggtgaa
     1921 gtaggtagaa ggccaacctg tttgaaatga atgcaatatt gatagataca tgacatacta
     1981 cagttgattt taaaactact gtgccttaac atgtttctta agcttttgta gtaagtgaac
     2041 ctttgagatc cattttgata aacctgctgt caatgtttcc cttatgtatg ttttctaact
     2101 ttgtcttggt aattgcaatt taactaggtg cggtggctac taaagttcga aggcacgata
     2161 tgcgtgtcca tccttaccaa aggattgtga ccgcagaccg aggttagttt agttctgcag
     2221 ttcttgttta taagcaatgt gctgggtgtc caaaaagttt gcaacaccag acctgatgga
     2281 aaaatgtaac tgtctacctg cacaccattt tcttttcttt ttccctttct aaatttaata
     2341 attgattgat ttaaggttgg tttggttttg tggttttgtt tttggtttgg gttttcaccc
     2401 ctccccttta cattttaatt tattaaatct tctattactt tgtcctaaga tttattccag
     2461 ctaaaacata aattagtcta tttggggagc tattaaattt ttacattatc atagcaatgt
     2521 ttagtaataa ttgttgctaa atttttccct ttaattattc tgaatatttc agttggtact
     2581 gcaaaatgaa ttgagcagat gatgttttta tgagagaagt aaaaaaaaaa aattttgctt
     2641 ccatgtaatt ttggagaaaa ctgaagattt attatccatt tttctatatt ataaaacatt
     2701 gagccactat ataaatatga gggtatatgt gtcaagaaca tacctaaaat caacagtcag
     2761 gactgtgaag ataattttaa tctaagtgga tactttatga atagaaaaca agttccctct
     2821 tactctattt tctggccaaa acggatgctt tgagttttgt catttgctgc taaattacag
     2881 tacctgaacg ttaactgtcc tgcagttctg tggtatgcga ggtccagctc cttggatatc
     2941 cttttttact aacctgtgac tggaaacaag tcttcatttt tctcactttc atatttgtaa
     3001 cttgtgccct atatatgccc ttggacccat ttttaaatta tttatgctta tttgagtagt
     3061 agtagttttg tttgggtttt ttttttttgt tttgttttgt ttttgttttt tttttttttg
     3121 ttttgacttt tttgtgtgat tcttttgtgt tttgttttta attgaaagat tggaatccca
     3181 agcatagtag aaaaggagct agctcacttg acatggttac tctactcttg aataaccact
     3241 ataaatacgg cacacagttg ccttgtgtat tagaaataaa ataaccttcc aaagacatca
     3301 gtgatactta tttactatca cactaaaata gagtcctgaa aactttacct aacatatatt
     3361 tttgtagtgg tttttcattg aacaaaatct ggtggcatat gcataacaat actactacat
     3421 gcagttgtgc caagtgtgag catatcaacc attgtatcag agtcgcatag agatgaggaa
     3481 gttagatggg aaaggttact tcagatgtgg gcagtctcat agatactttg aagctgcatc
     3541 atactgcaaa agtctaacat aattatttta cagagtgttt tgattttgaa gtaatttttc
     3601 cccctctttg gtttttcttt ccccgtgttc cttttaaatt ttttcactaa cacaataatc
     3661 ctttctcact ttgtactcca gagccttcag atagttgaga atattagact aagatcttac
     3721 tgtatttgga aaatgacaac acattccatg catggctcat ctggagctgt gtttattttg
     3781 attagttctg tttgtgttca aaatgcagtg tcagcaaacc aaaacggttt ttatcccccc
     3841 tgctccatct ccaacttttc ctttcttcta gagatagatc acttaggaac acgatcacca
     3901 catgtactca ctgggtccaa gggagaggac cgaagccctt gacttacatt attttatgat
     3961 actactgttt ctctctctct ctctctctct ctctctctct ctttctctct ctctctctct
     4021 ctctcatctt ttgagaagat tattattagc attcatttga tgcaaggaag cagcccgagg
     4081 acatataacc atgtaactgt ctgtatgtaa gcacttagag tatctagttg cttactaaaa
     4141 acaaacccac ttccatagtt atttcacaat tggtttgaaa cttaaagacc aactgacttt
     4201 atagttgtaa cgaactatac gtgattttgc acagttaaca ttacctgtgt gtactgtagg
     4261 agtccaggac atggcagagg atgtatttta gatgtactgg atatgataat gtactttgtt
     4321 aagtaatcca cactcaaata taatcagaaa ttatctaagt aatgatttga ccaaaacatt
     4381 acttacacaa atttaagact atgggacaac cgtttttgtt ttaaataaaa gtctgcttcc
     4441 ttttaagaaa ttcagggaag atttgattat attgaagata caatattttt gtagtattta
     4501 taaactgtcc taattgatag actatagaaa ccatttttgt catacaaagg taaataggtc
     4561 tattatatag aacatttaaa ccctgtgaac atgacaccta ctaggtttga tttatgaagt
     4621 ggcatagact ctaaccttgc actgagcatc ttttcattca catctctcct gcctttgtct
     4681 caatggcaac agtacagaaa attctgtcaa acctcagaat atagtagaaa taattaagct
     4741 gttgaatgag tcttaaaaat tatactactg ttaagtggac caagtttgat gaagtagaat
     4801 gtgagaggtt gattaaagaa ggagctactc aaggactttg gagtgataac ttttccactt
     4861 gagaactact ttatgtttta ctgtaatttt ttaaattttt ttttgttctt ttttgttact
     4921 ttgcaaaaga aaatagtatt tacaggtggc ttcttttaaa atataaaaat ataaagcagg
     4981 aatgtatatg aaatgtcaga ttttattgta tttgcagagt attagctttg aaattgaata
     5041 aagaaagctg tttgtagttt taaaaatgcc ttcttggtat cagttagaaa tttcttctat
     5101 tttttatgta cttagagttc tttgaggtat agagttttaa atggcaggat tttacagtgt
     5161 ttacatgcaa gtgcatttta taagtgttct atatgtgtaa attagtattt tcaactggaa
     5221 aatgttggct ggtgcaaaag gcctggcctt ttttctggtt cccatgatgt tgcctacact
     5281 gctagactac agtattgctt tgtatcatga ggccaagaaa ttccatgttg tttgtaaata
     5341 gaataattga aaaagcaata aacatttatt gaacaaaa
//
