LOCUS       NM_025574               5410 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus Pigy upstream reading frame (Pyurf), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_025574
VERSION     NM_025574.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Murakami,Y., Siripanyaphinyo,U., Hong,Y., Tashima,Y., Maeda,Y. and
            Kinoshita,T.
  TITLE     The initial enzyme for glycosylphosphatidylinositol biosynthesis
            requires PIG-Y, a seventh component
  JOURNAL   Mol Biol Cell 16 (11), 5236-5246 (2005)
   PUBMED   16162815
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK008978.1, AK032381.1, AC127307.3 and AK034566.1.
            
            On Mar 22, 2007 this sequence version replaced NM_025574.2.
            
            Summary: This gene encodes a small protein with a conserved DUF343
            domain. The human ortholog of this gene expresses two distinct
            proteins from upstream and downstream coding regions. The upstream
            CDS encoding a DUF343 domain-containing protein has been conserved
            at this mouse locus, but the downstream CDS encoding a subunit of
            an enzyme involved in glycosylphosphatidylinositol biosynthesis has
            not been conserved. Instead, a separate locus on mouse chromosome 9
            encodes the mouse homolog of the human phosphatidylinositol glycan
            anchor biosynthesis, class Y protein. [provided by RefSeq, Jul
            2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032381.1, AK011515.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AK008978.1         2-9
            9-939               AK032381.1         2-932
            940-5083            AC127307.3         95002-99145         c
            5084-5410           AK034566.1         2515-2841
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 27.82 cM"
     gene            1..5410
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /note="Pigy upstream reading frame"
                     /db_xref="GeneID:66459"
                     /db_xref="MGI:MGI:1913709"
     exon            1..270
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..412
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /note="protein preY, mitochondrial; pre-Y homolog"
                     /codon_start=1
                     /product="protein preY, mitochondrial precursor"
                     /protein_id="NP_079850.1"
                     /db_xref="CCDS:CCDS20184.1"
                     /db_xref="GeneID:66459"
                     /db_xref="MGI:MGI:1913709"
                     /translation="MLSATCRRLAPALRRLRALSAVAGRFLQVPGARLCSDQSERAEQ
                     PHTFHPALLQFLVCPLSKKPLRYEASTNELVNEELGIAYPIIDGIPNMIPQAARTTRQ
                     NEKQEEAEQP"
     exon            271..5410
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /inference="alignment:Splign:2.1.0"
     regulatory      781..786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /note="hexamer: TATAAA"
     polyA_site      805
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /note="major polyA site"
     regulatory      1087..1092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /note="hexamer: AATAAA"
     regulatory      1092..1097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /note="hexamer: AACAAG"
     polyA_site      1111
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
     polyA_site      1118
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
     regulatory      5389..5394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
                     /note="hexamer: AGTAAA"
     polyA_site      5406
                     /gene="Pyurf"
                     /gene_synonym="2610022G08Rik; Prey"
ORIGIN      
        1 agggtgacct ccgcggctct ggtgtgagag cagtgaagtt ccgagcgctg cttcgtccat
       61 cttctgagcc gccatgctga gcgccacgtg ccgcaggctc gccccggcgc tgcggaggct
      121 ccgcgcactg tctgcagtcg ccgggaggtt tctgcaagtg cccggggcca ggctctgctc
      181 tgaccagagc gagagggctg agcagcccca caccttccac ccggcgctgc tgcagttcct
      241 ggtgtgtccg ctctccaaga agccgcttag atatgaagca tcgacaaatg aattggttaa
      301 tgaagagttg ggaatagcat atccaatcat tgatgggatc cctaatatga taccacaggc
      361 agcaaggacc acacgtcaaa atgagaagca agaagaagct gagcaaccct agaccctgat
      421 tgttaaaagc ataattactg agttctctta attctgtaca cctttaacac agggagggag
      481 agttggggtg gaacagtaat tctgcctcgg cctgcttgac tgttcttccc ctgccctctt
      541 tagcaggact attctgatct gcttttctgg aggagaatgt cccacagggc tgcaccagca
      601 cagaccacag acaggctttg actttacagt gtgcttttgc caaccaccat agcaatgtgt
      661 gtgttctccc acctttggaa ccagaaaggt ctaaaactct ttaagtgtag ttagtacaac
      721 catagccggg acagcacgtg gattaataag agatctgcag caacatctgt gaaaactaca
      781 tataaaccca tttgatttat aacaagatct gtttgtctta tttctctcag gcttaggacc
      841 aggaagcagg ctatggaagc tttttcatgc atgttatagc cttgtttttt gaagatttca
      901 cttacaacca ctgtcagagg aagcatttca gtcatcagct gaggcttgct tcaaatccca
      961 ttcatttgaa agctggtgat ttcaagtatt gtttagtttc aaggtcaacg gtaagcttaa
     1021 gtgctaagaa aattctgaca ttgtaaactt gccttcagca ctttggtatt tcatggttac
     1081 atcaataata aaacaagatg ctcacagttt accaccaagc ccagtttgca gtggtggaag
     1141 gatgtctgct tgaatggatg gttttatctc aagagtctga taggaagaga acatgtgaaa
     1201 atagtttatg taactacatt ttctatacag agtcatttac tattcaaaat agaatcgttc
     1261 tccagggagg tcccttcagt aggtaaagtc cggagtggga agctggttat atccttttaa
     1321 gtttagtgtt tcaaagtaca ctccagtttc tcttgccctg gaaaatgtga agagtcctcc
     1381 ggcctttaac ctcagttttc tagaagtgta acagagctga ttgaggatga tatagtatct
     1441 acgttagaaa cagagcctga gtttctcagt ccttccccta aagaaatgca agaattgctt
     1501 ataaatttaa ttggttgcac atgccccggc ccaacgcctt gtgtatgtta ggcaagtgtg
     1561 tgaccactga aatcctctcc ccctgattta ttaatctact ctctgtgtgg ggaagagcca
     1621 tagcatgggg aagtcacaga acagattggt ggagttgatt ctttccttgc accattgggt
     1681 gatggagcct caagtcaggt ggccaggctt ggtggtgaga cctttatcat cctaattgtc
     1741 tcactggccc ttaattgatt cgtaatgagc tagcctccta gactagttta ataaaagatg
     1801 tacatgattc tccctgggaa agagaaaatt aaagcctgtc atacatgtct cctattttca
     1861 atattattaa gtataagttg cattcttata ttatccagtg gcaacacaga gctgaactag
     1921 atcatagagc ttctccattc ctgaagtgga gtttttagcc ctgaatttca catttaacca
     1981 actgaaagct gtggacaatg taaatgaaca gttttgccct taaagctagt gtcaacctga
     2041 taagtcagtt gcagcttttt taacttaatc atttgagcag ttgtctctaa gggagctcag
     2101 tatttattct ctcatcaaag aatctacttg tacaaagaac cctgggtgca gttattggca
     2161 tagtacagag tctttatttt cagaacctgg agaggaggta atagcacagt tttacagata
     2221 gacaaacaag catttcagta tcgacttgag ccagggagtt cctctgaatc ccagcacttg
     2281 agatgtgcag caggatgagg aagtcagtca aggacatctt gagctagaga actgtttatc
     2341 aaaactctaa tgaagcccat tgggtcagca agaagacaag taagaaaagg aaggtgtttc
     2401 aatggtaggg gcattaagag ggtactggga caaaaatgat cagaatacat tttatgcatg
     2461 gataaaatat tctattttgc ataattaata tgtttactca gaaaataata gtagatgtgt
     2521 cttagttggg gtttctattg ctgccatgaa atatgatgat caaaagcaag ttggggagga
     2581 aagggtttat ttggcttaca tttccatcac aatcatcaaa gaaggtcagg acaaaaacct
     2641 caaacagggc aggaacctgg aggcaggagc tgatatagat gctgctcact ggcttgctct
     2701 catggttatg ctcagtctaa ctacttcctt atagaaccca ggactagaat ggcaggtcgt
     2761 gtctcaatag cacactaggc caaggcagtt ccacatggaa gaggttttat tggaagggag
     2821 agacaaggta gtggcagaaa gagaagatgg gagaaagaga atgagaaagg ggcattctcc
     2881 ttatttatat ggaaaatgac gtaacacagg taaaggtgag aggtgagcca agtggatgct
     2941 agaaatattg gtgcctgttg gcaaccaatc tgcaggccgc gctgtcacct atgtgtgtta
     3001 tagatttggg atgtcctgat gtcaacaacc agcctatgga tggaaccact caatgggttg
     3061 gtccctaccc tattgatcac taagaaaatg ccctacaggt ttgccgagag cccagtctta
     3121 gggaggaatt ttctcaattg aggttccttc ctctcagatg attttagcat gtttcaagtt
     3181 gacaaaaact atccagcaca aggtaatacc agacacatct agctacccac gtttattgtt
     3241 ttctgtgagt ctgaataaac tctcaaggga acaggaccct ttcccaagaa taaagattta
     3301 agctaaccta acagataatg gctgtagtag taggcgcaaa atagcacgtt tgccttcatg
     3361 tttaataaaa atagtttaca ctactcacat caaggattca ttatatttcc tcaataatac
     3421 tacggtgttt ttttttttca aatgatggtt ttattgttcc aatagtacgt gatttaatgt
     3481 tatttttagt attttattta taattgtaaa agttgactaa gaaaaggtca gcgatacaca
     3541 atatgtccca tgaattaaaa ttcctgaata ttctatattt tcattctttg agtctacaag
     3601 gagataatat gggccatcct taccagttat ttcccaaccc aaactagctc ctttcatctt
     3661 tggtagtcca tctttttaat aggaagacct ctgagactca ggaatttaag gtactgttga
     3721 ttgacgtgac ctcagtgact cctgttgtag aaattaaaaa ggagtaacag tgataggctt
     3781 aagaatacat caaatttata agcatatgtg agtatattcc atgatagttt ttaaagtaaa
     3841 gtttttaatt ttcaccatta gcacttttga aatattgcca atagaattaa cacctgatgc
     3901 aaagtacaca ttcctgctgc aaagggagaa gggtatttct tgttattgca ttcatcagac
     3961 tgtgggaaat ctcaagggtt ctttttgcca tcaaatattg catacccact agacaactga
     4021 taatatgatg aagtggtaat caaggcagat gaacttcaac ctaaacagct tagaaaaatc
     4081 cttggtcttg tacatgtgtt cactggtgag aatgttctct aagttaactc caagatgtgt
     4141 ggctggtgtc tcattaatgg catccttgtt tgtgtttaat gagagtcttt attttattta
     4201 ttttgcttca taattctcaa tgtaagagat acatttaaat tctcatacac gttatgaatg
     4261 tgtctgcaag gtattcagtg ctagaaaata agtgttgcaa aatgatagtt atcagaacag
     4321 cacaggttta tgtctactta gtgtctgggt tgcgccatgg aagctaatta tttgtttatt
     4381 ctcatccagc agtcctaacc tgtgagttgt gacctgcatt atgattcata gcagtagcaa
     4441 aattatagtt ttgaagtagc aatgaaatag atttatggtt tggggtcact aacatgagga
     4501 accatgtcaa agggtcacag cattaagagg ttggacataa ggttgagaag cactggtctt
     4561 aggtatagat actggcccag atgtttggta tccacactaa catggaacac ttgtcttttc
     4621 taccttcata tctggttcag acaaagttca gaatttctct tacattttac tacagtggac
     4681 acatgaaaga tgtttaattc aggattctgg attttattgt agcttgatca caggggaaga
     4741 aatataaacg atttcaggta gaattgctac atgactaaaa ctggaggtcc aaaaatgttg
     4801 cataccttct ttccttgtaa aaggggtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     4861 tgtgctatta aaattttact tattagcaaa aacaaaacaa aaatctacaa gcaaacaaac
     4921 aaaaacaaaa acctgcctta aaatagcatt tacacattag atcaaaatac aacaatccag
     4981 aaagttggcc taagtcagtt ttcttcacta ctaaataaca ttcatcttca ttttgagaaa
     5041 atttctgttt acaaagaaaa taagaaccaa aatgtataga ttctaattcg ttaggctgtt
     5101 agtttttact gtctaaagta ccatatactt cagttaggag cattgaaact taatgttctg
     5161 tagcacaata gggtgactaa acttcatgac attttataaa gtacaagaaa tatccttaac
     5221 aaaatgttta tggacagggc cattagtctc cttatttgat catagtataa tgtgtacatg
     5281 tatcatgccc tttgagtata tgtaattttg tgtcaattta aaagacacct tatattttag
     5341 tttgttacat tatattatga ttgcctgctt tttatcttct gtctagacag taaactcttg
     5401 ttccaatttt
//
