LOCUS       NM_023258               2135 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus PYD and CARD domain containing (Pycard), mRNA.
ACCESSION   NM_023258
VERSION     NM_023258.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Saller,B.S., Wohrle,S., Fischer,L., Dufossez,C., Ingerl,I.L.,
            Kessler,S., Mateo-Tortola,M., Gorka,O., Lange,F., Cheng,Y.,
            Neuwirt,E., Marada,A., Koentges,C., Urban,C., Aktories,P.,
            Reuther,P., Giese,S., Kirschnek,S., Mayer,C., Pilic,J.,
            Falquez-Medina,H., Oelgeklaus,A., Deepagan,V.G., Shojaee,F.,
            Zimmermann,J.A., Weber,D., Tai,Y.H., Crois,A., Ciminski,K.,
            Peyronnet,R., Brandenburg,K.S., Wu,G., Baumeister,R.,
            Heimbucher,T., Rizzi,M., Riedel,D., Helmstadter,M., Buescher,J.,
            Neumann,K., Misgeld,T., Kerschensteiner,M., Walentek,P., Kreutz,C.,
            Maurer,U., Rambold,A.S., Vince,J.E., Edlich,F., Malli,R.,
            Hacker,G., Kierdorf,K., Meisinger,C., Kottgen,A., Jakobs,S.,
            Weber,A.N.R., Schwemmle,M., Gross,C.J. and Gross,O.
  TITLE     Acute suppression of mitochondrial ATP production prevents
            apoptosis and provides an essential signal for NLRP3 inflammasome
            activation
  JOURNAL   Immunity 58 (1), 90-107 (2025)
   PUBMED   39571574
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Jena,K.K., Mambu,J., Boehmer,D., Sposito,B., Millet,V., de Sousa
            Casal,J., Muendlein,H.I., Spreafico,R., Fenouil,R., Spinelli,L.,
            Wurbel,S., Riquier,C., Galland,F., Naquet,P., Chasson,L.,
            Elkins,M., Mitsialis,V., Ketelut-Carneiro,N., Bugda Gwilt,K.,
            Thiagarajah,J.R., Ruan,H.B., Lin,Z., Lien,E., Shao,F., Chou,J.,
            Poltorak,A., Ordovas-Montanes,J., Fitzgerald,K.A., Snapper,S.B.,
            Broggi,A. and Zanoni,I.
  TITLE     Type III interferons induce pyroptosis in gut epithelial cells and
            impair mucosal repair
  JOURNAL   Cell 187 (26), 7533-7550 (2024)
   PUBMED   39500322
REFERENCE   3  (bases 1 to 2135)
  AUTHORS   Wang,Y., Zhang,Y., Kim,K., Han,J., Okin,D., Jiang,Z., Yang,L.,
            Subramaniam,A., Means,T.K., Nestle,F.O., Fitzgerald,K.A.,
            Randolph,G.J., Lesser,C.F., Kagan,J.C., Mathis,D. and Benoist,C.
  TITLE     A pan-family screen of nuclear receptors in immunocytes reveals
            ligand-dependent inflammasome control
  JOURNAL   Immunity 57 (12), 2737-2754 (2024)
   PUBMED   39571575
REFERENCE   4  (bases 1 to 2135)
  AUTHORS   Ding,S.Q., Yan,H.Z., Gao,J.X., Chen,Y.Q., Zhang,N., Wang,R.,
            Li,J.Y., Hu,J.G. and Lu,H.Z.
  TITLE     Genetic deletion of the apoptosis associated speck like protein
            containing a card in LysM+ macrophages attenuates spinal cord
            injury by regulating M1/M2 polarization through ASC-dependent
            inflammasome signaling axis
  JOURNAL   Exp Neurol 382, 114982 (2024)
   PUBMED   39353545
  REMARK    GeneRIF: Genetic deletion of the apoptosis associated speck like
            protein containing a card in LysM[+] macrophages attenuates spinal
            cord injury by regulating M1/M2 polarization through ASC-dependent
            inflammasome signaling axis.
REFERENCE   5  (bases 1 to 2135)
  AUTHORS   Pizzuto,M., Hurtado-Navarro,L., Molina-Lopez,C., Soubhye,J.,
            Gelbcke,M., Rodriguez-Lopez,S., Ruysschaert,J.M., Schroder,K. and
            Pelegrin,P.
  TITLE     Ornithine lipid is a partial TLR4 agonist and NLRP3 activator
  JOURNAL   Cell Rep 43 (10), 114788 (2024)
   PUBMED   39340778
REFERENCE   6  (bases 1 to 2135)
  AUTHORS   Yamamoto,M., Yaginuma,K., Tsutsui,H., Sagara,J., Guan,X., Seki,E.,
            Yasuda,K., Yamamoto,M., Akira,S., Nakanishi,K., Noda,T. and
            Taniguchi,S.
  TITLE     ASC is essential for LPS-induced activation of procaspase-1
            independently of TLR-associated signal adaptor molecules
  JOURNAL   Genes Cells 9 (11), 1055-1067 (2004)
   PUBMED   15507117
REFERENCE   7  (bases 1 to 2135)
  AUTHORS   Mariathasan,S., Newton,K., Monack,D.M., Vucic,D., French,D.M.,
            Lee,W.P., Roose-Girma,M., Erickson,S. and Dixit,V.M.
  TITLE     Differential activation of the inflammasome by caspase-1 adaptors
            ASC and Ipaf
  JOURNAL   Nature 430 (6996), 213-218 (2004)
   PUBMED   15190255
  REMARK    GeneRIF: cell death triggered by stimuli that engage caspase-1 was
            ablated in macrophages lacking either ASC or Ipaf, suggesting a
            coupling between the inflammatory and cell death pathways
REFERENCE   8  (bases 1 to 2135)
  AUTHORS   Reed,J.C., Doctor,K., Rojas,A., Zapata,J.M., Stehlik,C.,
            Fiorentino,L., Damiano,J., Roth,W., Matsuzawa,S., Newman,R.,
            Takayama,S., Marusawa,H., Xu,F., Salvesen,G. and Godzik,A.
  CONSRTM   RIKEN GER Group; GSL Members
  TITLE     Comparative analysis of apoptosis and inflammation genes of mice
            and humans
  JOURNAL   Genome Res 13 (6B), 1376-1388 (2003)
   PUBMED   12819136
REFERENCE   9  (bases 1 to 2135)
  AUTHORS   Martinon,F., Hofmann,K. and Tschopp,J.
  TITLE     The pyrin domain: a possible member of the death domain-fold family
            implicated in apoptosis and inflammation
  JOURNAL   Curr Biol 11 (4), R118-R120 (2001)
   PUBMED   11250163
REFERENCE   10 (bases 1 to 2135)
  AUTHORS   Masumoto,J., Taniguchi,S., Nakayama,K., Ayukawa,K. and Sagara,J.
  TITLE     Murine ortholog of ASC, a CARD-containing protein, self-associates
            and exhibits restricted distribution in developing mouse embryos
  JOURNAL   Exp Cell Res 262 (2), 128-133 (2001)
   PUBMED   11139337
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DT921526.1, AK152677.1, CJ137522.1 and BG071362.2.
            
            On Jan 16, 2008 this sequence version replaced NM_023258.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK152677.1, AK018682.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               DT921526.1         1-141
            142-1405            AK152677.1         2-1265
            1406-1611           CJ137522.1         245-450
            1612-2135           BG071362.2         1-524               c
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.88 cM"
     gene            1..2135
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="PYD and CARD domain containing"
                     /db_xref="GeneID:66824"
                     /db_xref="MGI:MGI:1931465"
     exon            1..485
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             215..796
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="PYD and CARD domain-containing protein"
                     /codon_start=1
                     /product="apoptosis-associated speck-like protein
                     containing a CARD"
                     /protein_id="NP_075747.3"
                     /db_xref="CCDS:CCDS21888.1"
                     /db_xref="GeneID:66824"
                     /db_xref="MGI:MGI:1931465"
                     /translation="MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGAL
                     LQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVP
                     AQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKL
                     FSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS"
     exon            486..542
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..2135
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      875
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="major polyA site"
     regulatory      1387..1392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="hexamer: ATTAAA"
     polyA_site      1412
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
ORIGIN      
        1 caaaaagcca agtctgaaag tcttaggggc ggaaaccaaa actagtttgc tggggaaaga
       61 acaggagctg taagaaaaga gggtggggga gtcccagcat gcccatcggc ctaagcagct
      121 gacttcctgg tcttggcggg ctggcagcag gcaggctgca gcaggcgagc agcagcaaga
      181 gtaaaaggtg accgcggctg cccaccccag agccatgggg cgggcacgag atgccatcct
      241 ggacgctctt gaaaacttgt caggggatga actcaaaaag ttcaagatga agctgctgac
      301 agtgcaactg cgagaaggct atgggcgcat cccacgcggg gccctgctgc agatggacgc
      361 catagatctc actgacaaac ttgtcagcta ctatctggag tcgtatggct tggagctcac
      421 aatgactgtg cttagagaca tgggcttaca ggagctggct gagcagctgc aaacgactaa
      481 agaagagtct ggagctgtgg cagctgcagc cagtgtccct gctcagagta cagccagaac
      541 aggacacttt gtggaccagc acaggcaagc actcattgcc agggtcacag aagtggacgg
      601 agtgctggat gctttgcatg gcagtgtgct gactgaagga cagtaccagg cagttcgtgc
      661 agagaccacc agccaagaca agatgaggaa gctcttcagc tttgttccat cctggaacct
      721 gacctgcaag gactccctcc tccaggcctt gaaggaaata catccctact tggtgatgga
      781 cctggagcag agctgaggta tcttttccag ctacattatc tagctcctga ctttgtatac
      841 acaatttttg aaaaaacaat ttgtatttgt gtttactctc tgggatgttt ttgctgagct
      901 aagctacatt tccaaaggcc atatgtggcc cagtggtaga acatatacca aacatgcaca
      961 aatcagtctc caacacctaa attccacaga cccaagttat ggcttctcca ttctagtgca
     1021 aaggcttctc aaatgattcc tggctcccca tttgggagct tttcccatat tatcccaaaa
     1081 ggctaccttt gagaacacat gtaacattat ccaggtcaag ctaaaaagcg agaggggggg
     1141 atgggaacca ggtaatggtt aatcccagca accaggaggc agaggaggct gttccagaac
     1201 agccaagggt acagagagaa accctatctc aaaaaaacaa aaacaaaaaa aaaacaggca
     1261 atggggctta gaaaggcacc tagaaacaaa ccagtccgta gaagactttg ggctctggga
     1321 actcctgtct agacaaggct ggcctaactc aagagatctg cccagctctg cctcctgagt
     1381 tatgagatta aatgcatgta tcaccatgcc cagcccaagc cttttttttt ttttaattat
     1441 gtgtggattt tgggggtggg gctgttcata tgtgtgcacc atttcatgca atgccaacca
     1501 aagccagaag agggcatcag atcccctgga acaggagtta ctggctgagc ctccacacaa
     1561 tgctgggaat ttaacatggg tttcccctgg aaaaagcagt cgttacgact gcagaatcat
     1621 ctctccaacc ccaagggctt atcctttaaa gaaatgaaga tcgtattgtg tgaatataaa
     1681 cacgggtgca tgtctgcaca gaagcttgaa gacaaactcc agagttctgt ttcttacctt
     1741 gaggtaggga ggagttcctg ttctttttgc tgcatactaa ctctgggctg tgagcttcag
     1801 ggccattctg tttctctcat ctgtactttc aggtctctca gcagtgctga gattaacagg
     1861 tattgccatc attcagtgga cagaagacaa aaccagggtg aacgaagctc tctacaaccg
     1921 agtttcatcc ccagtccagt agctactctt ccaagtgtta tttatggtcc tacctatgga
     1981 attttttgaa tctcacttga cccctagatc cctgtcttaa ataggcctgg gatcaaaggc
     2041 ctaacaagtt cacgtgaaat cttattagtg attggagagg acaaaaaagg ttgcctttta
     2101 ttggtatgca cagtctatta gaagggtcct ttgtg
//
