LOCUS       NM_021534               2753 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus peroxisomal membrane protein 4 (Pxmp4), transcript
            variant 1, mRNA.
ACCESSION   NM_021534
VERSION     NM_021534.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Blankestijn,M., Bloks,V.W., Struik,D., Huijkman,N.,
            Kloosterhuis,N., Wolters,J.C., Wanders,R.J.A., Vaz,F.M.,
            Islinger,M., Kuipers,F., van de Sluis,B., Groen,A.K., Verkade,H.J.
            and Jonker,J.W.
  TITLE     Mice with a deficiency in Peroxisomal Membrane Protein 4 (PXMP4)
            display mild changes in hepatic lipid metabolism
  JOURNAL   Sci Rep 12 (1), 2512 (2022)
   PUBMED   35169201
  REMARK    GeneRIF: Mice with a deficiency in Peroxisomal Membrane Protein 4
            (PXMP4) display mild changes in hepatic lipid metabolism.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2753)
  AUTHORS   Fletcher,J.M., Jordan,M.A., Snelgrove,S.L., Slattery,R.M.,
            Dufour,F.D., Kyparissoudis,K., Besra,G.S., Godfrey,D.I. and
            Baxter,A.G.
  TITLE     Congenic analysis of the NKT cell control gene Nkt2 implicates the
            peroxisomal protein Pxmp4
  JOURNAL   J Immunol 181 (5), 3400-3412 (2008)
   PUBMED   18714012
  REMARK    GeneRIF: genetic sequences on chromosome 2 that control NKT cell
            numbers
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929557.15 and AL772292.10.
            
            On Mar 17, 2023 this sequence version replaced NM_021534.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1753940.1,
                                           SRR9219382.76253.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AL929557.15        7016-7171           c
            157-219             AL772292.10        199372-199434       c
            220-418             AL772292.10        195335-195533       c
            419-2753            AL772292.10        188928-191262       c
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 76.79 cM"
     gene            1..2753
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /note="peroxisomal membrane protein 4"
                     /db_xref="GeneID:59038"
                     /db_xref="MGI:MGI:1891701"
     exon            1..156
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..682
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /note="isoform 1 is encoded by transcript variant 1; 24
                     kDa intrinsic membrane protein; 24 kDa peroxisomal
                     intrinsic membrane protein"
                     /codon_start=1
                     /product="peroxisomal membrane protein 4 isoform 1"
                     /protein_id="NP_067509.1"
                     /db_xref="CCDS:CCDS16936.1"
                     /db_xref="GeneID:59038"
                     /db_xref="MGI:MGI:1891701"
                     /translation="MAAPPQLQALLQAVNKLLRQRRYHAALAVIKGFRNGAVYGVKIR
                     APHALVMTFLFRSGSLREKLQAILKATYIHSRNLACFVFAYKSLHALQSHVQGETHQM
                     HSFLAAFIGGLLLFGENNNINSQINMYLTSRVLYALCRLGVEKGYIPALKWDPFPLHT
                     AVIWGLVLWLFEYHRPTLQPSLQSSMTYLYEDSNVWHDLSDFLIFNKSHPSK"
     exon            157..219
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /inference="alignment:Splign:2.1.0"
     exon            220..418
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /inference="alignment:Splign:2.1.0"
     exon            419..2753
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      928
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /note="major polyA site"
     regulatory      2734..2739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /note="hexamer: ATTAAA"
     polyA_site      2753
                     /gene="Pxmp4"
                     /gene_synonym="3010018P03Rik; Pmp24"
                     /note="major polyA site"
ORIGIN      
        1 agttcttggc cagccaggac ccttgaccga gtgctccggt gctatggccg ccccaccgca
       61 gctacaggct cttctccagg ccgtcaacaa gctgctgcgc cagcgccgct accacgctgc
      121 gctggccgtg ataaagggct tccggaacgg ggctgtctat ggagtcaaaa tccgggcccc
      181 tcatgcactg gtgatgacct ttctcttcag gagcggcagt ctcagagaga agcttcaggc
      241 cattctgaaa gccacgtaca tccactctcg gaacctagcc tgctttgtgt ttgcctataa
      301 gagtctccat gccctacagt cccatgtgca aggcgagacc caccagatgc actctttcct
      361 ggctgccttc atcggggggc tcctgctgtt tggagagaac aataacatta atagccagat
      421 caacatgtac ctgacctcac gcgtcctgta cgccttgtgc cgcctgggtg tggagaaggg
      481 ctatatccct gcgctcaagt gggacccgtt ccccttgcac actgcggtga tttgggggct
      541 cgtgctgtgg ctttttgagt accaccggcc cactctgcag ccctccctac agtcctccat
      601 gacctacctc tacgaggaca gcaacgtgtg gcacgacctc tcagacttcc tcatcttcaa
      661 caagagccac ccctccaagt aacacagtcc aaggtgcttc aggaactcct ccgcccagac
      721 agcttccagg cagcagccca ccttccaggc agctgctcag ggtatggttc tgttggcata
      781 ccctcgtgga ggagcctgcc ttctcaaagt cataatcctc acactccaac ttgtattaac
      841 ccaggctccc atttgctgaa gtaaaagtac tgagttttcc acttaccagt acttttgagt
      901 actgtagcaa gtggctgggt cccattaagg agtctgtggc ttgattccag atgctcttcc
      961 gacttctgta aggccagggc aaactcaccc cctctcagct tcaggtcatt cacttcaggg
     1021 gaggaccctc cgaggagagt gtcttcctgg tggatatgat ggcccttccc ctctactgta
     1081 tatcagaggg ctccttgcta gtgggcaata ggttacaaac actttgaaag gggaaaaaaa
     1141 aactaaattc tttgtctaaa ttattctgct gagaagttct ggagccctac tgtcaaatag
     1201 gagcactaga tgagtcacgt gcacagcacg tgtcaagctt gtatgagacg caggatccta
     1261 tcaccagcac tgagaataaa acttcaagtg tccacatttt ttgagatgca ttaagaaggc
     1321 tgtggccggg cagtggtggc gcacgccttt aatcccagca ctcgggaggc agaggcaggc
     1381 agatttctga gttcgaggcc aacctggtct acagagtgag ttccaggaca gccagggcta
     1441 cacagagaaa ccctgtctcg gaaaaccaaa aaaaaaaaaa aaaaaggctg tggcagtagg
     1501 gttgtgagct ccaggccagg ctgcgataca ttaaaaaaca aacaaacaaa caacctctgt
     1561 ctcaaaaaaa ggcgggggtg ggcgtccaag gagatggctc agtgagtcaa gatggttgca
     1621 gccaagcctg gcgacctgag tttgatccct gtgttctaca tggtgacatg gtggaagaag
     1681 agaaccagct cctgcaagtt gtcctctgcc cccacatgtg tgccttggca tgtgtgcatg
     1741 cacacgtgca cacacacaca cacacacaca cacacacgca catatacaat aaataaatga
     1801 aaatataata aaacattaaa gaacatgtaa aattaatctt aatcatatat tttgcctgca
     1861 tgtacatctg tgtaccatgc gtgtgtgtgg taccagcaga agtcagcaga gggcgctgag
     1921 ttccttggaa ctgagggtat gggtgttgtg agccactatg tgggaaatga agcttggtct
     1981 tggtcgtctg caagagcact taagtttgtt gttgttgttg ttgttgttgt tgttgttgta
     2041 tgattgtttg aggttttttt tttttttttt tccccagaca gggtctctct gtgtagccct
     2101 ggctgtcctg gaattaactc tgtagaccag gctggcctcg aactcagact gcctctgcct
     2161 cccagtgttg ggattaaaga cgtggactgc ctggtgaact aagtgccttt caccactaag
     2221 ccatttctcc agcctcagac ctttaatcgt gtatatttta actcaatatt tcccaaactg
     2281 tgccattgct acatatgtta atataaaaga ttggtaacag cctttcttga agtatgtcct
     2341 caacactgcc agtgtgttct gcgtgcacca ggtcacacgg ttatcctcac agaaggttcc
     2401 ggaggacact gaaaatctgg aactaaagac acagtctgag ccctgccccc actctgtgat
     2461 gcatccccgg ggaaggctct ccaactctca gcacctccag ccctgctaac ccgatgccca
     2521 ccctggacac ttggcagcac ctgagagcca ctataccagt gacttcagca tagcgagttt
     2581 ctccttcctc ctgactgctt tgcatatgat tgtctttaat ccctagtaac cttgcaagga
     2641 tgcacgctga tccatgtttt ccaggtatgt ggtctcagga tgatttgaac agcctgcctg
     2701 actttgaagc ccgtgtttcc ttcagtgccc cgcattaaac ctagaaaacc tta
//
