LOCUS       NM_145458               2831 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus PX domain containing serine/threonine kinase (Pxk),
            transcript variant L, mRNA.
ACCESSION   NM_145458 XM_904662
VERSION     NM_145458.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Licatalosi,D.D., Yano,M., Fak,J.J., Mele,A., Grabinski,S.E.,
            Zhang,C. and Darnell,R.B.
  TITLE     Ptbp2 represses adult-specific splicing to regulate the generation
            of neuronal precursors in the embryonic brain
  JOURNAL   Genes Dev 26 (14), 1626-1642 (2012)
   PUBMED   22802532
REFERENCE   3  (bases 1 to 2831)
  AUTHORS   Gorokhova,S., Bibert,S., Geering,K. and Heintz,N.
  TITLE     A novel family of transmembrane proteins interacting with beta
            subunits of the Na,K-ATPase
  JOURNAL   Hum Mol Genet 16 (20), 2394-2410 (2007)
   PUBMED   17606467
REFERENCE   4  (bases 1 to 2831)
  AUTHORS   Mao,H., Ferguson,T.S., Cibulsky,S.M., Holmqvist,M., Ding,C., Fei,H.
            and Levitan,I.B.
  TITLE     MONaKA, a novel modulator of the plasma membrane Na,K-ATPase
  JOURNAL   J Neurosci 25 (35), 7934-7943 (2005)
   PUBMED   16135750
  REMARK    GeneRIF: MONaKA modulates brain Na,K-ATPase by binding tightly to
            its beta1 and beta3 subunits, and may thereby participate in the
            regulation of electrical excitability and synaptic transmission.
REFERENCE   5  (bases 1 to 2831)
  AUTHORS   Caenepeel,S., Charydczak,G., Sudarsanam,S., Hunter,T. and
            Manning,G.
  TITLE     The mouse kinome: discovery and comparative genomics of all mouse
            protein kinases
  JOURNAL   Proc Natl Acad Sci U S A 101 (32), 11707-11712 (2004)
   PUBMED   15289607
REFERENCE   6  (bases 1 to 2831)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK150660.1, AK153067.1, AK150337.1 and AW455875.1.
            
            On Jan 31, 2018 this sequence version replaced NM_145458.3.
            
            Transcript Variant: This variant (L) encodes the longest isoform.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016131.1, AK151098.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AK150660.1         2-45
            45-1020             AK153067.1         2-977
            1021-1569           AK150337.1         1004-1552
            1570-2809           AK153067.1         1527-2766
            2810-2831           AW455875.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 4.7 cM"
     gene            1..2831
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /note="PX domain containing serine/threonine kinase"
                     /db_xref="GeneID:218699"
                     /db_xref="MGI:MGI:1289230"
     exon            1..206
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..1853
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /note="long isoform is encoded by transcript variant L; PX
                     domain-containing protein kinase-like protein; modulator
                     of Na,K-ATPase"
                     /codon_start=1
                     /product="PX domain-containing protein kinase-like protein
                     long isoform"
                     /protein_id="NP_663433.2"
                     /db_xref="CCDS:CCDS26810.1"
                     /db_xref="GeneID:218699"
                     /db_xref="MGI:MGI:1289230"
                     /translation="MAFMEKPPAGKVLLDDTVPLTAAVEASQSLQSHTEYIIRVQRGI
                     SAENSWQIVRRYSDFDLLNNSLQITGLSLPLPPKKLIGNMDREFIAERQRGLQNYLNV
                     IMANHVLSNCELLKKFLDPNNYSANYTEIALQQVSMFFRSEPKWEVVEPLKDIGWRIR
                     KKYFLMKIKNQPKERLVLSWADLGPDKYLSDKDFQCLIKLLPSCVHPYIYRVTFATAS
                     ESSALLIRAFNEKGTLKDLIYKAKPKDPFLKKYCNPKKTQGLELQQIKTYGRQILEAL
                     KFLHDKGFPYGHLHAANVMLDGNTCRLLDLENSLLGLPSFYRSYFTQFRKINTLESVD
                     VHCFGHLLYEMTYGRPPDSVPVDSFPPASSLAVVAVLESTLSCEACKNGMPTVSRLLQ
                     MPLFSDVLLTTSEKPQFKIPTKLKEALRIAKECIEKRLTEEQKQIHQHRRLTRAQSHH
                     GSEEERKRRKILARKKSKRSAVENSEEQPVKHSNSNNSAGSGASSPLTSPSSPTPPST
                     AGLSSALPPPPPPPPPPPPPAGPSPTSATEMPAPFLPQPVNGVNRGALLSSIQNFQKG
                     TLRKAQTCDHSAPKIG"
     exon            207..257
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            258..305
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            306..492
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            493..570
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            571..644
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            645..719
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            720..824
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            825..926
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1088
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1206
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1285
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1334
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1412
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1499
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1569
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1632
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..2815
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2794..2799
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /note="hexamer: AATAAA"
     polyA_site      2812
                     /gene="Pxk"
                     /gene_synonym="C230080L11Rik; D14Ertd813e; Monaka; Slob"
                     /note="major polyA site"
ORIGIN      
        1 gcggtgcgcg ttgacagcag cggcggcggg acgcggcggg tggcggggct cggcgcctcc
       61 ggttcctgcc ccggcttcag aggcagcggc ggcggcggcc cgggatggcc ttcatggaga
      121 agccgccggc tggcaaggtg ctgctggacg acacggtgcc gctgacagcg gccgtcgagg
      181 cgagccagag tctgcagtcg cacacggaat atatcatccg ggtgcaaaga ggaatttctg
      241 cagaaaacag ctggcagatc gtcagaagat acagtgactt cgatctgctt aacaacagct
      301 tgcaaattac aggcctgagc ctgcctcttc ctcctaagaa actgattgga aacatggacc
      361 gtgagttcat cgctgagagg cagcgaggcc tgcagaacta ccttaacgtc atcatggcaa
      421 atcatgtctt gtccaactgt gagctgctta agaagttcct agacccaaac aactattctg
      481 caaactatac tgagatcgcc ttacagcagg tttccatgtt cttccgatca gaaccaaagt
      541 gggaggtggt agaaccactg aaggacatag gttggaggat aaggaagaaa tatttcttga
      601 tgaaaataaa aaaccaacca aaggagcggc tggtgctaag ctgggctgat cttggcccag
      661 acaagtactt gtcagataaa gattttcagt gtctaatcaa acttctgcct tcctgtgtgc
      721 acccttacat ctatcgggtc acctttgcca cggctagcga gtcctccgca ttgctgatta
      781 gagcctttaa tgagaaggga actttgaagg atctgatcta caaggcaaaa ccaaaggacc
      841 ccttcctaaa gaaatactgc aaccctaaga aaacccaggg ccttgaactc cagcaaataa
      901 aaacatatgg gcggcagata ttagaggcac taaagtttct tcatgacaag ggattccctt
      961 acggccacct ccatgcagcc aatgtgatgc tggatggcaa cacttgccgg ctgctagacc
     1021 tcgaaaactc actgctgggt ctgccttcct tctaccgatc ctacttcaca caattcagga
     1081 aaatcaatac attggagagt gtagatgtgc actgttttgg ccacttactc tatgaaatga
     1141 cttatggacg acctcccgac tccgtgcctg tggactcctt tcctcctgca tcatccttgg
     1201 ctgtggtggc cgtattggag tctacgctgt cttgtgaagc ctgtaaaaat ggcatgccta
     1261 ccgtctcccg gctcttacag atgccattat tcagtgatgt tttactaaca acttctgaaa
     1321 agccacagtt taagattccc acaaaactaa aagaggcatt gcgaattgcc aaggaatgca
     1381 tagagaagag actcaccgag gaacagaagc agattcacca gcatcgaaga ctaacgagag
     1441 cccagtcaca ccatgggtct gaggaagaaa ggaaaaggag aaagatctta gctcgaaaga
     1501 agtcaaaacg atctgctgtt gaaaacagtg aggagcagcc agtgaagcac agtaactcca
     1561 ataactcagc aggatctggg gccagctcac ctctcacatc cccatcatct ccaactccac
     1621 cctccacagc aggactgtct tcagcattac ctccacctcc tccgccacct cctcctccac
     1681 ctccaccagc aggtccctcg ccaacctcag ccacggagat gcctgctccg ttcctgcccc
     1741 agcctgtgaa tggtgtgaac cgtggggcct tgctcagttc catccagaat ttccaaaagg
     1801 gaactttgcg gaaagcccaa acctgtgatc acagcgctcc caagatcggc tgatgcttct
     1861 tggtaacgct gagcaggctc agccctctgc cccgtcccac ccctcttcct aggtgagctc
     1921 ttccacagct tccctcaagg ccttccccgg gtgactccct gcagagccag ccacactcca
     1981 gttaggaggc ttgaagaggg aagtgcgctc caaatagaat acagtggggc cagcactgct
     2041 gccttaatat tttgctcctt gtgatgctat tgggcttcct ttaggggcag cactgtttgg
     2101 gtattatttt caccagcgaa agaaaaatgt cagaaaatca aaagcaatgg cagtattctg
     2161 tcacagcagc aattggcctt tgtcactccg tccttcccac tctattatag aagtgttctc
     2221 atagctcatt cctctgcagc atcttttggt tagttcttag gcagctcacc tcagatgcta
     2281 acgttgtaac ctgtcagtta cactgctgag ggagaaacag cggctcatca ctctccctac
     2341 acagaggatg cgggggagga gacgggggag ctcctcagag ctcagtgtgg actgaactct
     2401 gttcactgtg ctcagaacag cacctcggga tctgtacagc cacaatgcgc cagacaggac
     2461 attaaccaca gataaagggt tgtcttagtc ctcagccatt aaatccagct tggagtagag
     2521 ggagggccaa cattacaagt gaatctggca tctatagtaa tcacttctga tttgatttat
     2581 ccaacttaag catcttttga tattactgtt atcagaaatc ttttatccta tgatttgctt
     2641 aaacctttta aataaattgc actttaaagg attataaaaa atccatttta aaattcaaat
     2701 acacacatca gtgttggtta ctatgcagaa agaatgttat tgtgtataga ttcatgtaat
     2761 ctgtgatata tgttcagctg tattttttat taaaataaat catgtcacga aagtaaaaaa
     2821 aaaaaaaaaa a
//
