LOCUS       NM_001098636            2640 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus PWWP domain containing 2B (Pwwp2b), transcript variant
            1, mRNA.
ACCESSION   NM_001098636
VERSION     NM_001098636.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Yan,L., Jin,W., Zhao,Q., Cui,X., Shi,T., Xu,Y., Li,F., Jin,W.,
            Zhang,Z., Zhang,Z., Tang,Q.Q. and Pan,D.
  TITLE     PWWP2B Fine-Tunes Adipose Thermogenesis by Stabilizing HDACs in a
            NuRD Subcomplex
  JOURNAL   Adv Sci (Weinh) 8 (16), e2102060 (2021)
   PUBMED   34180153
REFERENCE   3  (bases 1 to 2640)
  AUTHORS   Zhang,T., Wei,G., Millard,C.J., Fischer,R., Konietzny,R.,
            Kessler,B.M., Schwabe,J.W.R. and Brockdorff,N.
  TITLE     A variant NuRD complex containing PWWP2A/B excludes MBD2/3 to
            regulate transcription at active genes
  JOURNAL   Nat Commun 9 (1), 3798 (2018)
   PUBMED   30228260
  REMARK    GeneRIF: Study in HeLa, HEK293F, and mouse embryonic stem cells
            reveal that loss of PWWP2A/B leads to increases in histone
            acetylation predominantly at highly expressed genes, accompanied by
            decreases in Pol II elongation. Collectively, these findings
            suggest a role for PWWP2A/B in regulating transcription through the
            fine-tuning of histone acetylation dynamics at actively transcribed
            genes.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC116669.1, AK158245.1 and AK213732.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK165809.1, SRR7974084.25842.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-534               BC116669.1         1-534
            535-1531            AK158245.1         535-1531
            1532-1922           AK213732.1         1-391
            1923-2640           AK158245.1         1618-2335
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 84.39 cM"
     gene            1..2640
                     /gene="Pwwp2b"
                     /gene_synonym="D7Ertd517e; D930023J19Rik; Pwwp2"
                     /note="PWWP domain containing 2B"
                     /db_xref="GeneID:101631"
                     /db_xref="MGI:MGI:2142008"
     exon            1..162
                     /gene="Pwwp2b"
                     /gene_synonym="D7Ertd517e; D930023J19Rik; Pwwp2"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..1840
                     /gene="Pwwp2b"
                     /gene_synonym="D7Ertd517e; D930023J19Rik; Pwwp2"
                     /note="isoform 1 is encoded by transcript variant 1; PWWP
                     domain containing 2; PWWP domain-containing protein 2B"
                     /codon_start=1
                     /product="PWWP domain-containing protein 2B isoform 1"
                     /protein_id="NP_001092106.1"
                     /db_xref="CCDS:CCDS52427.1"
                     /db_xref="GeneID:101631"
                     /db_xref="MGI:MGI:2142008"
                     /translation="MEPRAGCRLPVRVEQVVNGALVVTVSCGERSFAGILLDCTKKSG
                     LFGLSPSTLLPLADNSSAVSCHGQAPEEGTGEVMQLETGPLHPHHKDPEKDQPPKTAV
                     SEPPPPLIPPVPAGNLPPFPPYFEGAPFPHPLWLRNTYQQWVPQPPPRTIKRTRRRLS
                     RNRDPGRLILSTIRLRPRQVLCEKCKSTVSPQEASPSPLNTPKPRRRLGSGPDSEHRK
                     PEEPEDSAVIATAAPRRSKREKREEDRVAGERVPRSPVIKISYSTPQGKGEVVKIPSR
                     VHGSVEPFCPQQSLQNGSQDSEVSRDVEPRGGGDRPPSGSSASIPKLKLTRPVPPISD
                     LPPPKIRLKPHRLGDGEHEPLYRAELVEELNGCPRGPLVSSPALFADGSSHGLEDLSS
                     GSSGEDDDLKRFPQGKHGRDGLAFLVDCPGRRTDCTSESVCSTDSLDELKSSGSEVTS
                     PDTGDLSSGDSASVPSSSADTRQTVPPLTVRLHTQSVSRCVTEDGRTVAVGDIVWGKI
                     HGFPWWPARVLDISLGQKEDGEPSWQEAKVSWFGSPTTSFLSISKLSPFSEFFKLRFN
                     RKKKGMYRKAITEAANATQHVAPEIRELLTQFEM"
     exon            163..1858
                     /gene="Pwwp2b"
                     /gene_synonym="D7Ertd517e; D930023J19Rik; Pwwp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..2640
                     /gene="Pwwp2b"
                     /gene_synonym="D7Ertd517e; D930023J19Rik; Pwwp2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2552..2557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pwwp2b"
                     /gene_synonym="D7Ertd517e; D930023J19Rik; Pwwp2"
                     /note="hexamer: AAGAAA"
     polyA_site      2574
                     /gene="Pwwp2b"
                     /gene_synonym="D7Ertd517e; D930023J19Rik; Pwwp2"
                     /note="major polyA site"
ORIGIN      
        1 gtggcgggcg ggggcggcct ccggaggcgg cgggagcatg gagccgcggg ccggctgccg
       61 gttgccggtg cgggtggaac aggtcgtcaa cggcgcgctg gtggtcaccg tgagctgtgg
      121 cgagcgcagc ttcgccggaa tcctgctgga ttgcacgaaa aagtctggcc tctttggcct
      181 gtctccatct actctactgc ctctggctga caactcctct gccgtcagct gtcatggcca
      241 ggctcccgag gagggaactg gagaagtgat gcagctagag acaggacctc tgcatcctca
      301 ccacaaagac cctgagaagg atcagcctcc caagacagct gtgtctgagc cgcccccacc
      361 tctcatacca cctgtgcctg ccgggaacct gcctcccttt ccaccatact ttgagggtgc
      421 ccccttccct cacccactat ggctgaggaa cacctaccag cagtgggtgc cgcagccccc
      481 acccaggacc atcaagcgga cccgtcggcg actctccaga aaccgggatc ctggaagact
      541 cattcttagc acaatccgcc tgcggccacg tcaggtactg tgtgagaagt gcaagagtac
      601 tgtgagtcct caagaggcca gtcccagccc cctgaacacc cccaaacccc gccggaggct
      661 aggcagtggc cctgacagtg aacaccgaaa gccagaggaa ccagaagaca gcgctgtcat
      721 agctacagct gccccgagga ggagcaagag ggagaagcgg gaagaggaca gggttgctgg
      781 agagcgtgtt ccgagaagcc cagtcatcaa gatctcctac agcacaccac agggtaaggg
      841 tgaggtggtc aagattccat ccagagtgca tggctccgtg gagcccttct gcccgcaaca
      901 gtctctacag aatggcagcc aggactcaga ggtatcaagg gatgtggagc ccaggggtgg
      961 tggagaccga ccacccagcg ggtcttcagc ctccatcccc aagctaaagc tgactcgccc
     1021 agtgcctcct atctctgacc tgccacctcc caaaatccgc ctgaagcccc accggctggg
     1081 ggatggagag catgaacccc tttaccgggc tgagctggtg gaggagctga acggatgccc
     1141 acgaggcccg ctggtcagct cccctgctct ctttgccgat ggctctagcc atgggctaga
     1201 ggacttgtct tctggaagtt ctggtgagga cgatgacctc aagaggtttc cccaaggtaa
     1261 acatggccgt gatggcttgg cttttcttgt ggactgccct gggaggagaa cagattgtac
     1321 cagtgaatct gtgtgcagca cggacagcct ggatgaatta aaatcatctg gttcagaagt
     1381 gacatctcca gacacaggag acctgtcatc tggtgacagt gcctctgttc cttcatcttc
     1441 tgctgacact cgccagacag tccctcccct cacggtcagg ctgcacacgc agagcgtctc
     1501 acggtgtgtg actgaagatg gaaggacggt ggctgtaggg gacattgtgt ggggtaagat
     1561 tcatggtttt ccttggtggc cagcgcgtgt ccttgacatc agccttggcc agaaggagga
     1621 tggagagccc tcttggcaag aagcgaaagt ctcatggttt gggtctccaa ctacatcatt
     1681 cttgtctatt tcaaaactgt cccctttctc tgaatttttc aaactgagat ttaaccgtaa
     1741 gaagaagggg atgtatcgga aagctataac tgaggctgca aatgccacac aacacgtggc
     1801 cccagaaata agggagctct tgacccagtt tgaaatgtaa ctttggttcc aagagcaggt
     1861 caccgacaat gagggtgcag ctgctgtcct ggaacttctg acttctgtcc cagggaccca
     1921 cgtgtctctg gctggctgtg tgcagaaaca tgctgggcac agtggctggc ctgcctccag
     1981 aggagcaggt gtgcatgagg agggacccag cttagggcac ctggctcctc tcggctgaat
     2041 gtatctgttc aggcttcagc gttcctagcc gagaagtgtc ctggagcttg gtgacatgtt
     2101 tgctgctttg tgtgctagag gctgcaaagc ttaggcatcc gcaaacagaa acagggcttt
     2161 ctgcccagcc tgtgaaactc ctggtgtggg tgctgggcct agctacaggg catcctcctg
     2221 tgcctggcct ccgttattca gcacgagctg ccctataggc tttgaggaaa ccaaattgca
     2281 cgtactgggt tctcctccag cccttgggta tagggtctct gtgcacttga gccaggaacc
     2341 tagtcgcatc tcttccacat tccactcaaa gcatgggtcc ctggctccca gccccagcct
     2401 gcaaggccgc cttggtctga gatgctgcta tgtcaccagg actgggaagg ggagaaggaa
     2461 ggcagaagtg aaaacagacc ccaggttagg agataccgtc tctgccctgt gaggccacat
     2521 tcctttcccc tctgtcactt ccgagaccaa aaagaaaaca gaaagaaaag aaaactgtct
     2581 ctgctccttt gtctaagcat ggaaagaaat aaacaggagt ccgtgttgag gctcttgcct
//
