LOCUS       NM_027557               3251 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus PWWP domain containing 2A (Pwwp2a), transcript variant
            2, mRNA.
ACCESSION   NM_027557 XM_001481283 XM_282969
VERSION     NM_027557.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Wei,G., Brockdorff,N. and Zhang,T.
  TITLE     The PWWP2A Histone Deacetylase Complex Represses Intragenic
            Spurious Transcription Initiation in mESCs
  JOURNAL   iScience 23 (11), 101741 (2020)
   PUBMED   33235983
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3251)
  AUTHORS   Zhang,T., Wei,G., Millard,C.J., Fischer,R., Konietzny,R.,
            Kessler,B.M., Schwabe,J.W.R. and Brockdorff,N.
  TITLE     A variant NuRD complex containing PWWP2A/B excludes MBD2/3 to
            regulate transcription at active genes
  JOURNAL   Nat Commun 9 (1), 3798 (2018)
   PUBMED   30228260
  REMARK    GeneRIF: Study in HeLa, HEK293F, and mouse embryonic stem cells
            reveal that loss of PWWP2A/B leads to increases in histone
            acetylation predominantly at highly expressed genes, accompanied by
            decreases in Pol II elongation. Collectively, these findings
            suggest a role for PWWP2A/B in regulating transcription through the
            fine-tuning of histone acetylation dynamics at actively transcribed
            genes.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3251)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   5  (bases 1 to 3251)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732633.6.
            
            On Nov 18, 2022 this sequence version replaced NM_027557.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK173305.1, SRR13422603.75256.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-590               AL732633.6         46016-46605
            591-3251            AL732633.6         68523-71183
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 25.81 cM"
     gene            1..3251
                     /gene="Pwwp2a"
                     /gene_synonym="4631424J17Rik; D930040F23Rik"
                     /note="PWWP domain containing 2A"
                     /db_xref="GeneID:70802"
                     /db_xref="MGI:MGI:1918052"
     exon            1..590
                     /gene="Pwwp2a"
                     /gene_synonym="4631424J17Rik; D930040F23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..2259
                     /gene="Pwwp2a"
                     /gene_synonym="4631424J17Rik; D930040F23Rik"
                     /note="isoform b is encoded by transcript variant 2; PWWP
                     domain-containing protein 2A"
                     /codon_start=1
                     /product="PWWP domain-containing protein 2A isoform b"
                     /protein_id="NP_081833.1"
                     /db_xref="CCDS:CCDS48774.1"
                     /db_xref="GeneID:70802"
                     /db_xref="MGI:MGI:1918052"
                     /translation="MAAVAAEAAATAASPGEGGAGEAEPELEPIPGSEAGTPLPVTAT
                     EAAVPDGEADGRQSAPQADEQPLPPPPPPPPPGELADSSEAEEAKPPEPAAVPVSPPE
                     QPPAAPEQPEDAPRPPPAPALVPPAGGDSAVSHLIPGSEVRVTLDHIIEDALVVSFRL
                     GEKLFSGVLMDLSKRFGPHGIPVTVFPKREYKDKPDAMQLQSTTFQEGIEVKQEVNGA
                     VPDDLSPVPPPERLWASKPPPLFHEGAPYPPPLFIRDTYNQSIPQPPPRKIKRPKRKM
                     YREEPTSIMNAIKLRPRQVLCDKCKNSVVAEKKEIRKGSSDSSRYEDKKRRNDSVATV
                     NKKLKTDHKVDGKNQNESQRRNTVVRVSSIAHSRGRVVKVSAQANTSKAQLNTKKVLQ
                     SKNMDHAKAREVLKIAKEKAQKKQSETSTSKTAHAKVHFTRRYQSPSSGSLPPRVRLK
                     PQRYRNEENDSSLKTGLEKIRSGKLAPKPQSRCTSTRSAGEAPSEKPSPSEGPEESAG
                     EVQDTSRVRVPGEQEELRMLGKKGSKSSISVYLTLNQETSDSSSASVCSIDSMDDLKS
                     SNSECSSSESFVFPPGCMHAPSASSTSTSFSSKEENSLRNSLKMKIFSKNVSKCITPD
                     GRTICVGDIVWAKIYGFPWWPARILTITVSRKDNGLLARQEARISWFASPTTSSLALS
                     QLSPFLENFQLRFNKKRKGLYRRAITEAAKAAKQLTPEVRALLTQFET"
     exon            591..3251
                     /gene="Pwwp2a"
                     /gene_synonym="4631424J17Rik; D930040F23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3230..3235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pwwp2a"
                     /gene_synonym="4631424J17Rik; D930040F23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3251
                     /gene="Pwwp2a"
                     /gene_synonym="4631424J17Rik; D930040F23Rik"
                     /note="major polyA site"
ORIGIN      
        1 atcccagccg cgctgccgcc gccgccgccg cagcccgagt aggaggaggg agaaaccagc
       61 gagaaaatgg cggccgtggc tgctgaggcg gcggcgaccg cggcgtcccc cggggaaggg
      121 ggcgccggcg aggccgagcc ggagctagag cccattcccg gcagcgaagc cggcaccccc
      181 ctcccggtca cggccacgga ggcggcggtg ccggacggcg aggccgacgg gcgccagtcc
      241 gcccctcagg ccgacgagca gccgctgccg ccaccgccgc cgccgccgcc gccgggggag
      301 ctggccgata gctccgaggc tgaggaggcg aagccgcccg agcccgcggc tgtcccggtc
      361 tcgccgcccg agcagcctcc ggccgccccc gagcagcccg aggacgcgcc gcggccgcca
      421 ccagctccgg cgctcgtgcc gccggcgggc ggggactcgg cggtgtcgca cctcatcccc
      481 ggctcggagg tgcgggtcac gctggaccac atcatcgagg acgcgctcgt cgtgtcgttc
      541 cgcctcgggg agaagctctt ctccggggtc ctcatggacc tatccaaaag gtttgggccc
      601 catgggatcc ctgtgacagt atttcccaaa agggaatata aggacaaacc ggacgccatg
      661 cagctccaaa gtaccacatt ccaagaaggg atagaagtca agcaggaagt gaacggtgct
      721 gttcccgatg acctctctcc agtccctcct cctgagcgcc tgtgggcttc caagccacct
      781 cctctcttcc atgaaggagc accttaccct ccccccttgt ttatcaggga cacatataac
      841 caatcaatac ctcagccgcc gcctcggaaa attaagcgac ccaaacgaaa aatgtacagg
      901 gaagaaccca cttcaataat gaatgcgatt aagctacggc ccaggcaagt cctgtgtgat
      961 aagtgtaaaa acagtgttgt tgctgaaaag aaggaaatta ggaaaggtag cagtgactct
     1021 tctaggtatg aagataaaaa acggagaaat gacagtgtag ctactgtgaa caaaaaactg
     1081 aaaactgacc ataaagtgga tgggaaaaac caaaacgaaa gccagagaag gaacactgtg
     1141 gtgagggttt ccagtatcgc tcacagcagg ggcagagtag tcaaggtttc tgctcaggca
     1201 aatacatcaa aagctcagtt aaataccaag aaagtgctcc aaagcaagaa catggatcac
     1261 gcaaaagctc gggaagtatt gaaaattgcc aaagaaaagg cacagaagaa gcaaagtgaa
     1321 acctccactt ccaaaaccgc ccatgcgaaa gtccacttca cacgtcggta tcagagtccc
     1381 agctcaggtt ctctcccacc ccgagtgcgg ttaaaaccac aaaggtacag gaacgaagaa
     1441 aatgactctt ctctgaagac gggactggag aaaattcgga gtggcaagct ggcgcctaag
     1501 ccgcagtctc gatgcacctc cacccgctca gcaggtgagg ccccttcaga aaaaccgagt
     1561 ccctcagaag gcccggaaga gtccgccggt gaggttcagg acacaagcag agtgcgtgtg
     1621 cctggtgagc aggaggaact gcggatgttg ggcaaaaagg gcagcaaaag cagtatctct
     1681 gtttacctga ccctaaatca ggagacatct gactcttcca gtgcctcagt gtgtagcatt
     1741 gatagcatgg atgatttgaa atcctccaac tctgagtgta gctcttctga gagcttcgtt
     1801 tttcctcccg gctgtatgca cgcaccttct gcctcttcca cttctacttc cttctcttca
     1861 aaggaagaga atagcctccg taattctttg aaaatgaaaa tcttttcaaa aaatgtctct
     1921 aaatgtatca caccagatgg caggaccata tgtgtagggg acattgtttg ggccaagata
     1981 tatggcttcc cttggtggcc agcccgtatt cttaccatca ctgtaagccg gaaagataac
     2041 ggccttttag cccggcagga ggcccgtatc tcatggtttg catctccaac cacatcttcc
     2101 cttgctctct cacaactctc cccctttcta gaaaacttcc agttacggtt taataagaag
     2161 agaaagggtc tgtatcgcag ggctatcaca gaggctgcta aggctgctaa gcagctgacc
     2221 cctgaagtgc gggctttgtt gacacaattt gaaacgtgaa catggacagt aaggtaggcg
     2281 agaaccactg gagctgctgc aggtccctgg ctagtcagga atcatttcca ccagctagct
     2341 tggccaaact ggtgagaaat tgcactcagt tggcttttta tacgttatat atagccattt
     2401 ttacactaag aagctgaaaa ctgaaagatg tttcaaattt tctcttaagt aggattttag
     2461 gaatattttc cagttttaaa atctaaaacc accctaaggc atagaagcca tagcttcaaa
     2521 tgaaacaatt tttgcaggga ctgttaattg ccatttgtag ctattgtttg tgtgtatatg
     2581 tatgtctagg tatgcacacc tagacataca tacacacaca tccacatgta taaactattg
     2641 tctgtcacca tgtgacatgg gttgcatatt tgagatttta gtaagggctg gaacttgggg
     2701 aacagtggat attcaatgat tacttgtttt atctagtgaa aacttagact ttctatgcac
     2761 atagggtata aaagtttgat attggatgtt tgacacactg agatgcttgc tatgacaaat
     2821 tggtatgctt gctgtttagt tatagaccag gctgtacaca ttgccagtat ttgggattaa
     2881 aactattaag actagacagg tttgggtcag tactctcttg tccagtgagg ttgtaaaaca
     2941 aactgtcaga tatttggtgg gaagtttaga tagattttat ttggggtata taaataattg
     3001 agtgaaaagt tagtcagatt tctcctgact ggctgttcct aaattcttag gacagaccct
     3061 agcaatttac actttgtgaa ttaatgtcat gtgtaattgt tgcattttgg atgtacctaa
     3121 tttgatagtt tttcagaagc attttccatt taaggtaatc tgtttgcagg tcagaaaatg
     3181 agaaatgtga ttgtataatg cattgaaatg taaaaaaaaa aaagctgtaa ataaaatcaa
     3241 ctaaatccaa a
//
