LOCUS       NM_001401141            3390 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus pseudouridylate synthase 10 (Pus10), transcript
            variant 8, mRNA.
ACCESSION   NM_001401141 XM_006514861
VERSION     NM_001401141.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Wang,Y., Zhang,Z., He,H., Song,J., Cui,Y., Chen,Y., Zhuang,Y.,
            Zhang,X., Li,M., Zhang,X., Zhang,M.Q., Shi,M., Yi,C. and Wang,J.
  TITLE     Aging-induced pseudouridine synthase 10 impairs hematopoietic stem
            cells
  JOURNAL   Haematologica 108 (10), 2677-2689 (2023)
   PUBMED   37165848
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3390)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3390)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   5  (bases 1 to 3390)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672049.13, BX255874.1 and AL805916.7.
            
            On Feb 3, 2022 this sequence version replaced XM_006514861.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927932.1622620.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-239               AL672049.13        210245-210483
            240-376             AL672049.13        211308-211444
            377-625             AL672049.13        216538-216786
            626-712             AL672049.13        217268-217354
            713-747             BX255874.1         21926-21960
            748-859             BX255874.1         24886-24997
            860-921             AL805916.7         4015-4076
            922-967             AL805916.7         4653-4698
            968-1032            AL805916.7         5888-5952
            1033-1118           AL805916.7         8326-8411
            1119-1244           AL805916.7         9330-9455
            1245-1301           AL805916.7         14692-14748
            1302-1378           AL805916.7         15706-15782
            1379-1434           AL805916.7         15879-15934
            1435-1552           AL805916.7         17234-17351
            1553-1695           AL805916.7         22562-22704
            1696-1834           AL805916.7         26066-26204
            1835-3390           AL805916.7         28451-30006
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 14.34 cM"
     gene            1..3390
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /note="pseudouridylate synthase 10"
                     /db_xref="GeneID:74467"
                     /db_xref="MGI:MGI:1921717"
     exon            1..239
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            240..376
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     CDS             251..1873
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /EC_number="5.4.99.25"
                     /note="isoform 2 is encoded by transcript variant 8;
                     putative tRNA pseudouridine synthase Pus10; psi55
                     synthase; tRNA-uridine isomerase; tRNA pseudouridylate
                     synthase; tRNA pseudouridine 55 synthase; coiled-coil
                     domain-containing protein 139; coiled-coil domain
                     containing 139"
                     /codon_start=1
                     /product="tRNA pseudouridine synthase Pus10 isoform 2"
                     /protein_id="NP_001388070.1"
                     /db_xref="GeneID:74467"
                     /db_xref="MGI:MGI:1921717"
                     /translation="MLPLTEENKHVAQLLFSSGTCPRCILRFCGVDLPAPYKHPSKEL
                     LNELQKFLEPEKPELILEAPNPPLKKIRLHEDGIDNLSEDGKEGVSVTEDESMAEKPS
                     KLRVCNVCLGILQEFCEKGFITKVCQKVEASGFEFTSVVLSVSFPPQLSVREHAAWLL
                     VKQEMGKQSLSLGRNDVVQLKEAYKWITHPLFSEELGVPTDGKSLFEVSVVFAHPETA
                     EDCHFLGEVCRDCFKPAKNKQSVFTRMAVLKALSKIKEEDFLGQFPCPPNSPKTVCTV
                     LEVECTHGAVFVAGRYNKYSRNLPQTPWIIDGERKMESSVEELISDHLLAVFRAESFN
                     FSSSGREDVDVRTLGNGRPFAVELLNPHRVHFTSQEMKELQQTINKSSDKIQVRDLQL
                     VTREAIGHMKEGEEEKTKTYSALIWTNRAIQKKDIGFLDDLKDLKIDQKTPLRVLHRR
                     PLAVRTRAIHSMKTHYLDEHHFRLHLKTQAGTSWYCIIFSLKRPSYIKEFVHGDFGRT
                     KPNLGSLMNVTADILELDVESVDVDWPPALDD"
     exon            377..625
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            626..712
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            713..747
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            748..859
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            860..921
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            922..967
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1032
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1118
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1244
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1301
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1378
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1434
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1552
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1695
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1834
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..3390
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3029..3034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /note="hexamer: AATAAA"
     polyA_site      3052
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /note="major polyA site"
     regulatory      3369..3374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /note="hexamer: ATTAAA"
     polyA_site      3390
                     /gene="Pus10"
                     /gene_synonym="2810013G11Rik; 4933435A13Rik; Ccdc139"
                     /note="major polyA site"
ORIGIN      
        1 ctttcgttcg tagagctagg gggagtgcag gctgccaggc ggacagcctt acaggtcacc
       61 gtgtaagacg agggggttcc tgcattcctc tcgtgagggg cctggtactt tcgttgtaaa
      121 gctctggttt tcttggggaa cacaacggct gagcgcagta ggtgcgctgt ctttactctt
      181 ccccgttccc acccagggcc gaaaagggtt aagactcggc tgcggcatct tgggagaagt
      241 ttcttctagt atgcttccac tgactgagga gaacaagcac gtggcacagc tgttgttcag
      301 ttctggcacc tgtccaagat gcatcttaag attctgtggt gtggatcttc ccgcacctta
      361 caaacaccca tccaaggagt tgctcaatga gctacaaaaa tttctagaac cggaaaaacc
      421 tgaattaatt ttagaagctc caaacccacc attgaagaaa attcgtctac atgaagatgg
      481 gattgataat ttgagtgaag atggaaagga gggagtctct gttactgaag atgaaagcat
      541 ggctgagaag ccttcaaagc tgagagtgtg caatgtttgc ttaggaattc ttcaggaatt
      601 ctgtgagaag gggtttatta cgaaggtgtg ccaaaaggtt gaagcctctg ggtttgaatt
      661 caccagcgtg gttttatctg tctcctttcc accgcagctg tctgtaagag agcatgctgc
      721 atggttgctg gtaaaacagg aaatgggaaa gcagagtctg tccctgggaa gaaatgatgt
      781 agtccagctg aaagaagcct acaagtggat aactcacccc ctgttttcag aggagctggg
      841 cgttcccact gatggaaaga gcttgttcga agtgagcgtg gtctttgctc atccagaaac
      901 agctgaagat tgccatttcc taggtgaagt ttgccgagat tgtttcaaac cagctaaaaa
      961 taaacagtcg gtatttacca gaatggcagt tttgaaagct ttgagtaaga taaaagaaga
     1021 agacttcctc gggcaatttc cttgtcctcc aaactcaccg aagactgtat gcactgtcct
     1081 tgaagttgag tgcactcatg gtgctgtttt tgtcgctggc agatataata aatactccag
     1141 gaatctacca caaactcctt ggataattga tggagaaagg aaaatggaat cttcggtgga
     1201 agaattaatt tcagatcatc ttctggcagt attcagagca gagagtttta atttctcatc
     1261 ctcgggacga gaagacgtag acgtgagaac attaggaaat ggaaggccct ttgcagttga
     1321 acttctgaat cctcatcgag tgcatttcac ctcacaagaa atgaaggagc tccagcagac
     1381 aattaataaa tcatctgaca aaatccaagt ccgtgacttg cagcttgtca ccagggaggc
     1441 aatagggcat atgaaggaag gtgaggaaga aaagactaag acctacagtg ccttgatatg
     1501 gacgaataga gctatccaga agaaggacat tgggttcctg gacgacttaa aggacttaaa
     1561 gattgaccag aaaacacctc tccgagtcct tcaccgaagg cctttggctg tgagaactcg
     1621 ggccattcac tctatgaaga cacactacct ggatgagcat cattttcgtc tgcatttgaa
     1681 gacccaagct ggaactagtt ggtattgcat aatcttttcc ttaaagagac ctagctacat
     1741 taaagaattt gtacatggag actttgggag aaccaaacca aaccttggct ctttgatgaa
     1801 cgtgactgca gacattcttg agctggatgt ggagtctgta gatgttgact ggccgcctgc
     1861 tctggatgac tagctcccac gtgtgaacac aaaaggcttt cctggcatga tgtggacata
     1921 caggcagcat atcctggaaa atgactgttt acccaccata acggtgtctt caaaaccact
     1981 tggatcatgt tgatctgttc ctaaagttca ctgtaacatc tcaggatcta tttgtacgca
     2041 tattttgtgt tgtgttgtgt tgttctaaga atgtcttatg ttttctcatt ccctgtctca
     2101 tgaccacaaa accaaaatag aaggaatgaa accattttcc ttctaaagtc tgttacttag
     2161 agagagatgt aatgggaggc cctgtcagtc aggagagctg ggacttctag cataatctcc
     2221 tgcaggggat gataccttca aagtaacttg ctaaagcttt cagatcatct gaataaatgt
     2281 tacaactaga ctcatgtaga agagtgtcct ataatattcc aatatgtcaa atgtaaatct
     2341 gattctaata ttttccagtt actctcaaac aagcttctat tatcaataga agtactttaa
     2401 ggaacaattt gtcatagtcc ttatgacctc aagtgactat tttgacaatc gaaaattgtc
     2461 agtatgtgtc tcctgcaaag caagtgacac cttacccctg gaggcgtact ctagacactg
     2521 cctacctctt ttgtctttta tgtaaagaga aacaagtgcc ttcagtaact tttgtcccag
     2581 agttacaaat ttgaaaattc gataatgcta aacaaataca aacatatttt ggtgtttcaa
     2641 ttattttcca atttcaaatg ttttctaatt tcaagtggtc tatctaacat ctgattacat
     2701 aaacaaggaa acaaaagtta aatgacacac ctagttactc ttataacagg tctggagctg
     2761 atgttgtgtc tccaggagcc tttctaaaaa cccttgtcag gagcacctgc atctcctgtg
     2821 ggtgacagtg tcctaagaag gacagttagg cttacaaagc caggaggttg gcttctggaa
     2881 cttggatatc aagtgacgcc cccaagaaat ggctaatggt atgtcttata tgatctcata
     2941 attgtcaatt gtaatggcat ggtgaagaga aatcttaaac atattgaacc agaagaatta
     3001 atcatcaaaa ataccagaag ggaagctcaa taaaattttg acttaagtaa aattttcatc
     3061 agctagtgca acttcttgac tttttttgat gcattgattt gaatagcatt tgattattca
     3121 cgtttctcat ctttgcacac aattcatact gactctgaaa gatgtattat atcaggaaga
     3181 tgtttttgat tgcgatgaaa gggcagcctg ctcctttgaa accttgtttc atcatgtgtg
     3241 aataatagca aagggttttt cattgtcaga aaattgaaaa ggagataaca gaaactaact
     3301 gatgaaaagt gaaaagttaa agacttctat gtaatgtttc tcagcttctg atgaaatttt
     3361 gctaaaagat taaataattt gggaaaagtc
//
