LOCUS       NM_019700               1838 bp    mRNA    linear   ROD 08-AUG-2024
DEFINITION  Mus musculus pseudouridine synthase 1 (Pus1), transcript variant 2,
            mRNA.
ACCESSION   NM_019700
VERSION     NM_019700.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Shi,D., Wang,B., Li,H., Lian,Y., Ma,Q., Liu,T., Cao,M., Ma,Y.,
            Shi,L., Yuan,W., Shi,J. and Chu,Y.
  TITLE     Pseudouridine synthase 1 regulates erythropoiesis via transfer RNAs
            pseudouridylation and cytoplasmic translation
  JOURNAL   iScience 27 (3), 109265 (2024)
   PUBMED   38450158
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1838)
  AUTHORS   Mangum,J.E., Hardee,J.P., Fix,D.K., Puppa,M.J., Elkes,J.,
            Altomare,D., Bykhovskaya,Y., Campagna,D.R., Schmidt,P.J.,
            Sendamarai,A.K., Lidov,H.G., Barlow,S.C., Fischel-Ghodsian,N.,
            Fleming,M.D., Carson,J.A. and Patton,J.R.
  TITLE     Pseudouridine synthase 1 deficient mice, a model for Mitochondrial
            Myopathy with Sideroblastic Anemia, exhibit muscle morphology and
            physiology alterations
  JOURNAL   Sci Rep 6, 26202 (2016)
   PUBMED   27197761
  REMARK    GeneRIF: Collectively, these results suggest that alterations in
            muscle metabolism related to mitochondrial content and oxidative
            capacity may account for the reduced exercise capacity in Pus1(-/-)
            mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1838)
  AUTHORS   Zhao,X., Patton,J.R., Ghosh,S.K., Fischel-Ghodsian,N., Shen,L. and
            Spanjaard,R.A.
  TITLE     Pus3p- and Pus1p-dependent pseudouridylation of steroid receptor
            RNA activator controls a functional switch that regulates nuclear
            receptor signaling
  JOURNAL   Mol Endocrinol 21 (3), 686-699 (2007)
   PUBMED   17170069
  REMARK    GeneRIF: Pus1p- and Pus3p-dependent pseudouridylation of SRA
            controls a coactivator-corepressor switch in SRA with major
            consequences for NR signaling.
REFERENCE   5  (bases 1 to 1838)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   6  (bases 1 to 1838)
  AUTHORS   Zhao,X., Patton,J.R., Davis,S.L., Florence,B., Ames,S.J. and
            Spanjaard,R.A.
  TITLE     Regulation of nuclear receptor activity by a pseudouridine synthase
            through posttranscriptional modification of steroid receptor RNA
            activator
  JOURNAL   Mol Cell 15 (4), 549-558 (2004)
   PUBMED   15327771
  REMARK    GeneRIF: Results identify a pseudouridine synthase, mPus1p, as a
            coactivator for retinoic acid receptor (mRAR)gamma when coexpressed
            with steroid receptor RNA activator.
REFERENCE   7  (bases 1 to 1838)
  AUTHORS   Chen,J. and Patton,J.R.
  TITLE     Mouse pseudouridine synthase 1: gene structure and alternative
            splicing of pre-mRNA
  JOURNAL   Biochem J 352 Pt 2 (Pt 2), 465-473 (2000)
   PUBMED   11085940
REFERENCE   8  (bases 1 to 1838)
  AUTHORS   Chen,J. and Patton,J.R.
  TITLE     Pseudouridine synthase 3 from mouse modifies the anticodon loop of
            tRNA
  JOURNAL   Biochemistry 39 (41), 12723-12730 (2000)
   PUBMED   11027153
REFERENCE   9  (bases 1 to 1838)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   10 (bases 1 to 1838)
  AUTHORS   Chen,J. and Patton,J.R.
  TITLE     Cloning and characterization of a mammalian pseudouridine synthase
  JOURNAL   RNA 5 (3), 409-419 (1999)
   PUBMED   10094309
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY331510.1, BU515425.1, CB059120.1 and CF618003.1.
            
            On Apr 13, 2006 this sequence version replaced NM_019700.3.
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            coding region, as compared to variant 1. The encoded isoform 2 thus
            lacks an internal segment, as compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034359.1, AK089258.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-335               BY331510.1         2-336
            336-809             BU515425.1         308-781
            810-1282            CB059120.1         165-637
            1283-1838           CF618003.1         27-582              c
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.7 cM"
     gene            1..1838
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /note="pseudouridine synthase 1"
                     /db_xref="GeneID:56361"
                     /db_xref="MGI:MGI:1929237"
     exon            1..357
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /inference="alignment:Splign:2.1.0"
     CDS             278..1549
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /EC_number="5.4.99.12"
                     /note="isoform 2 is encoded by transcript variant 2; tRNA
                     pseudouridine synthase A, mitochondrial; tRNA-uridine
                     isomerase I; tRNA pseudouridylate synthase I; tRNA
                     pseudouridine(38-40) synthase; tRNA pseudouridine synthase
                     1; pseudouridylate synthase 1 homolog"
                     /codon_start=1
                     /product="pseudouridylate synthase 1 homolog isoform 2"
                     /protein_id="NP_062674.2"
                     /db_xref="CCDS:CCDS19531.1"
                     /db_xref="GeneID:56361"
                     /db_xref="MGI:MGI:1929237"
                     /translation="MGFPRLWAALLRNWGRWTARPGPRVPGLPPMAGNKVPPALASHQ
                     PDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGS
                     SQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLID
                     DILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDESY
                     RLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFA
                     VIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERV
                     HFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPI
                     HNFSGTALGAADTGAKVPSSLEGSEGDGDTD"
     exon            358..568
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /inference="alignment:Splign:2.1.0"
     exon            569..706
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /inference="alignment:Splign:2.1.0"
     exon            707..809
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /inference="alignment:Splign:2.1.0"
     exon            810..1501
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1814
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1788..1793
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /note="hexamer: ATTAAA"
     polyA_site      1814
                     /gene="Pus1"
                     /gene_synonym="A730013B20Rik; MPUS1; mPus1p"
                     /note="major polyA site"
ORIGIN      
        1 gtgcacgtgg tcagcttcgg gggttccgcg gcgccgtgag tgggtccggc gggactttcc
       61 tccctcctcg agcgcgccgg ctgcgcgatc tctaaggacg gggtggtggt gttggatggg
      121 ggtgggcagg ggctgcgggg gtcacaggtc agcctaggat ctggggcgcc gcagggtcag
      181 agccaagaca tctggaagac agggcaggga tttcggggct gcagagttcg gaggggatcg
      241 ctgaggtccg gggtcggtgc ccgccacccc gcagcgcatg ggcttccccc gcctgtgggc
      301 cgcactgctg agaaactggg gacggtggac ggcgcgccca ggcccgcgcg tgcccggcct
      361 gccgcctatg gctggaaaca aggtgccacc agcgctggcc tcacaccaac cggacaggaa
      421 gggccgaggt ggctgggtct gggaggagac ggagcatcct gcaaagaggg tcaagggcgg
      481 cgaggatgaa gagcccccac gcaagctgcc aaagcgaaaa attgtgctgc tcatggccta
      541 ctcgggcaag ggctaccacg gcatgcagag gaatctgggg tcctcccagt tcaggaccat
      601 cgaagatgac ttggtatcgg ccttagtcca ggcaggttgt atccctgaaa accatggcac
      661 agatatgagg aagatgtcct tccagcggtg tgctcgaaca gacaagggtg tgtctgcagc
      721 agggcaggtg gtatccctaa aggtgtggct gattgatgac attctggaca agatcaacag
      781 ccaccttcca tcccacattc ggattctggg attgaagagg gtcacaggcg ggttcaactc
      841 caagaacaag tgtgacgcca ggacctactg ctacatgctg cccacctttg cctttgctca
      901 caaggaccgg gatgtacagg acgagagcta tcgcctgagt gcagagaccc tgcagcaggt
      961 caaccgcctt ctggcctgct acaaaggcac ccataacttc cataacttca cctcgcagaa
     1021 gggcccaaga gagcccagtg cgcgccgcta cattctggag atgtactgcg aagagccctt
     1081 cgtacgtgag ggcctggagt ttgctgtgat caaggtgaag ggccagagct tcatgatgca
     1141 ccagatccgg aagatggttg gcctggtggt agccattgtg aagggttatg cccctgagag
     1201 tgtcctggag cgcagctggg gagaggagaa ggtggatgtg cccaaggcac ccgggcttgg
     1261 cttagtcctg gagagggttc actttgaaaa gtacaatcaa cgctttggcg gtgacgggct
     1321 gcatgagccc ctggactgga cgcaggagga agggaaggtg actgctttca aggagcagta
     1381 catttaccct accattgtca gcactgagcg ggatgagcgg tccatggccc agtggctcaa
     1441 tactttgccg atccacaact tcagtggcac tgcacttgga gcggctgaca caggtgccaa
     1501 ggtccccagt tccctggagg gcagtgaagg ggatggagac actgactgaa actgtagatc
     1561 ctgccactgc tgtgtgtgct gaggctggaa tctctgcacc tgcctgtcac tcaggacggg
     1621 ggggagccaa gaattcgggg gctggcttgt ggatgtagcc tggcttcata acctcaggat
     1681 attaaattct catcccagga atcagctgcc gactttagcc catgcatcgt gtgtatacac
     1741 cttagcatga aagggcactt tttaaaaaaa gtgatttttt ttttcctatt aaatatgttt
     1801 tgcttagtga attaaaaaaa aaaaaaaaaa aaaaaaaa
//
