LOCUS       NM_001160220            6305 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus pumilio RNA-binding family member 2 (Pum2), transcript
            variant 3, mRNA.
ACCESSION   NM_001160220
VERSION     NM_001160220.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Meng,Y., Chen,L., Chai,Y., Meng,W., Yang,G., Ren,J., Li,H., Qi,P.,
            Chen,J. and Wang,N.
  TITLE     PUM2 promoted osteoarthritis progression through PTEN-mediated
            chondrocyte ferroptosis by facilitating NEDD4 mRNA degradation
  JOURNAL   Environ Toxicol 39 (9), 4318-4332 (2024)
   PUBMED   38733337
  REMARK    GeneRIF: PUM2 promoted osteoarthritis progression through
            PTEN-mediated chondrocyte ferroptosis by facilitating NEDD4 mRNA
            degradation.
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6305)
  AUTHORS   Gu,J., Lu,J., Yang,J., Liu,Y., Zhu,X., Zhang,J., Shen,H., Li,X.,
            Yu,Z. and Li,H.
  TITLE     Norad Competently Binds with Pum2 to Regulate Neuronal Apoptosis
            and Play a Neuroprotective Role After SAH in Mice
  JOURNAL   Neuroscience 535, 108-123 (2023)
   PUBMED   37913857
  REMARK    GeneRIF: Norad Competently Binds with Pum2 to Regulate Neuronal
            Apoptosis and Play a Neuroprotective Role After SAH in Mice.
REFERENCE   4  (bases 1 to 6305)
  AUTHORS   Zhi,K., Yin,R., Guo,H. and Qu,L.
  TITLE     PUM2 regulates the formation of thoracic aortic dissection through
            EFEMP1
  JOURNAL   Exp Cell Res 427 (2), 113602 (2023)
   PUBMED   37062520
  REMARK    GeneRIF: PUM2 regulates the formation of thoracic aortic dissection
            through EFEMP1.
REFERENCE   5  (bases 1 to 6305)
  AUTHORS   Yoon,D.S., Choi,Y., Lee,K.M., Ko,E.A., Kim,E.J., Park,K.H. and
            Lee,J.W.
  TITLE     Downregulation of the RNA-binding protein PUM2 facilitates
            MSC-driven bone regeneration and prevents OVX-induced bone loss
  JOURNAL   J Biomed Sci 30 (1), 26 (2023)
   PUBMED   37088847
  REMARK    GeneRIF: Downregulation of the RNA-binding protein PUM2 facilitates
            MSC-driven bone regeneration and prevents OVX-induced bone loss.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6305)
  AUTHORS   Moore,F.L., Jaruzelska,J., Fox,M.S., Urano,J., Firpo,M.T.,
            Turek,P.J., Dorfman,D.M. and Pera,R.A.
  TITLE     Human Pumilio-2 is expressed in embryonic stem cells and germ cells
            and interacts with DAZ (Deleted in AZoospermia) and DAZ-like
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 100 (2), 538-543 (2003)
   PUBMED   12511597
REFERENCE   7  (bases 1 to 6305)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 6305)
  AUTHORS   Spassov,D.S. and Jurecic,R.
  TITLE     Cloning and comparative sequence analysis of PUM1 and PUM2 genes,
            human members of the Pumilio family of RNA-binding proteins
  JOURNAL   Gene 299 (1-2), 195-204 (2002)
   PUBMED   12459267
REFERENCE   9  (bases 1 to 6305)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13061-13066 (2002)
   PUBMED   12226475
REFERENCE   10 (bases 1 to 6305)
  AUTHORS   White,E.K., Moore-Jarrett,T. and Ruley,H.E.
  TITLE     PUM2, a novel murine puf protein, and its consensus RNA-binding
            site
  JOURNAL   RNA 7 (12), 1855-1866 (2001)
   PUBMED   11780640
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ124920.1, AK141682.1, AK147617.1 and BQ828592.1.
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            donor splice site in the 3' coding region compared to variant 1.
            This results in an isoform (2) lacking 2 aa compared to isoform 1.
            Variants 3 and 4 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK141682.1, SRR1660813.193559.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-400               CJ124920.1         1-400
            401-4439            AK141682.1         263-4301
            4440-6272           AK147617.1         4388-6220
            6273-6305           BQ828592.1         1-33                c
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 3.92 cM"
     gene            1..6305
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /note="pumilio RNA-binding family member 2"
                     /db_xref="GeneID:80913"
                     /db_xref="MGI:MGI:1931751"
     exon            1..222
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            223..291
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..3435
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /note="isoform 2 is encoded by transcript variant 3;
                     pumilio homolog 2; pumilio 2"
                     /codon_start=1
                     /product="pumilio homolog 2 isoform 2"
                     /protein_id="NP_001153692.1"
                     /db_xref="CCDS:CCDS49025.1"
                     /db_xref="GeneID:80913"
                     /db_xref="MGI:MGI:1931751"
                     /translation="MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDE
                     WRETAWGTSHHSMSQPIMVQRRSGQSFHGNSEVNAILSPRSESGGLGVSMVEYVLSSS
                     PADKLDSRFRKGTFGTRDAETDGPEKGDQKGKASPFEEDQNRDLKQDDEDSKINGRGL
                     PNGMDADCKDFNRTPGSRQASPTEVVERLGPSTNPPEGLGPLPNPTANKPLVEEFSNP
                     ETQNLDAMDQVGLDSLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTSALTVQQLT
                     AAQQQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATL
                     AGPAVVPPQYYGVPWGVYPANLFQQQAAAAASNTANQQAASQAQPGQQQVLRPGAGQR
                     PITPSQGQQGQQAESLAAAANPTLAFGQSLAAGMPGYQVLAPTAYYDQTGALVVGPGA
                     RTGLGAPVRLMAPTPVLISSTAAQAAAAAAAAGGTANSLTGSTNGLFRPIGTQPPQQQ
                     QQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSASLGFGSGSSLGAAIGSA
                     LSGFGSSVGSSASSSATRRESLSTSSDLYKRSSSSLAPIGQPFYNSLGFSSSPSPIGM
                     PLPSQTPGHSLTPPPSLSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSASSTSSL
                     FSSSSQLFPPSRLRYNRSDIMPSGRSRLLEDFRNNRFPNLQLRDLIGHIVEFSQDQHG
                     SRFIQQKLERATPAERQIVFNEILQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALATR
                     IRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVLKCVKDQNGNHVVQKCI
                     ECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQL
                     VQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAER
                     ALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLR
                     KYTYGKHILAKLEKYYLKNSPDLGPIGGPPNGML"
     exon            292..400
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            401..588
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            589..755
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            756..1026
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1120
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1246
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1389
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1522
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1666
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1960
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2197
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..2465
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..2595
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2724
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2862
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            2863..2988
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3110
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            3111..3303
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     exon            3304..6298
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6214..6219
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /note="hexamer: AATGAA"
     regulatory      6242..6247
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /note="hexamer: AATAAA"
     polyA_site      6247
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /note="major polyA site"
     polyA_site      6269
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /note="major polyA site"
     regulatory      6270..6275
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
                     /note="hexamer: GATAAA"
     polyA_site      6298
                     /gene="Pum2"
                     /gene_synonym="5730503J23Rik; Pumm2"
ORIGIN      
        1 gttgttgtga gtctctgtgc ctcaccctcg gtacttcggt ggttcggagg aggaggggga
       61 ggagaagaag gaggtggggt gggggggagg gaggtaggag gaggagaaag aagacgtcgc
      121 cgccaccgga ggcggagggg gaggacggac ggccgagagc gtcgccgccg gtgcccaacg
      181 gtcggtgtcc cccggctgag gcagcgcggc cgcagcagcc ctgtttgatc tgtggatgaa
      241 atgaatcatg attttcaagc tcttgcatta gaatctcggg gaatgggaga gcttttgcct
      301 accaaaaagt tttgggaacc tgatgattca acaaaagatg gacaaaaagg catatttctt
      361 ggggatgatg aatggagaga gactgcatgg ggaacttctc atcattcaat gtcccagcct
      421 attatggtgc agagaagatc tggacagagt tttcatggaa acagtgaagt aaatgcaatt
      481 ctttctccac gctcagaaag tggaggcctt ggtgtgagca tggtagaata tgtattaagt
      541 tcctctcctg ctgataaatt ggattctcga tttaggaagg gaacttttgg tactagagat
      601 gctgaaacag atggacctga gaaaggagat caaaaaggca aggcttctcc atttgaggag
      661 gaccaaaaca gagatcttaa acaagatgat gaggactcta aaataaatgg cagaggtttg
      721 ccaaatggaa tggatgccga ttgcaaagat tttaatcgca ctcctggaag tcgccaagcc
      781 tctccaactg aagtagttga gcgccttggc cctagtacta atcccccaga aggattgggc
      841 cctcttccta atccgacagc gaataaacca cttgttgaag aattttcaaa ccctgaaact
      901 cagaatctgg atgcaatgga ccaagttggt ctggattcct tacagtttga ctatcctggt
      961 aatcaggtac ccatggattc ttcaggagct actgtaggcc tttttgacta caattcccaa
     1021 cagcagctct ttcagaggac tagtgcacta acagttcagc agttaactgc agctcagcag
     1081 cagcagtatg cattagcagc agctcagcag ccacatatag ctggtgtatt ctcagcaggc
     1141 cttgctccag ctgcatttgt gccaaatcca tatattatta gtgctgctcc tccagggact
     1201 gacccgtata ctgcagcagg attggctgca gcagcaacat tagcaggtcc agcagtggtt
     1261 ccacctcagt attacggtgt tccatgggga gtgtatccag ccaatttatt tcagcaacaa
     1321 gctgcagctg cggcaagcaa cacagccaac cagcaagcag catcacaagc tcagcctgga
     1381 cagcagcagg ttcttcgtcc tggagcaggt cagcgtccta ttactccaag tcagggccaa
     1441 caagggcagc aagcagagtc acttgcagca gctgcaaacc caactttggc ttttggtcag
     1501 agtcttgctg caggcatgcc aggctatcaa gtactagctc caactgccta ttatgatcag
     1561 actggtgcct tagtggttgg ccccggagca agaactggcc ttggagctcc agtacgatta
     1621 atggctccaa cacctgtctt aataagttca acagcagcac aagctgcagc agcagcagca
     1681 gcagctggag gaactgcaaa tagtcttaca ggcagcacaa atggtctgtt tcggccaatt
     1741 ggcactcagc caccacagca gcagcagcag cagcagcaac caagcactaa cctgcagtct
     1801 aattcatttt atgggagcag ctctttgact aacagctccc agagcagctc tttattctct
     1861 catggacctg gccaacctgg aagtgcgtct cttggctttg gaagtggtag ctctttagga
     1921 gctgctatag gctcagctct cagtggattt ggctcatcag ttggcagttc tgcaagtagt
     1981 agtgccacaa ggagagagtc tctatctact agctctgact tgtacaaaag atctagtagc
     2041 agcctagcac ccatagggca accattttac aatagtctgg gattttcctc ctctccaagt
     2101 ccaataggca tgcctctgcc aagccaaact ccaggacatt cacttacgcc accgccatca
     2161 ctttcatcac atggatcctc atccagtttg catttaggag gactgacaaa cggtagtggt
     2221 cggtatatct ctgcagcacc tggagcagaa gcaaagtacc gaagtgcttc aagcacttcc
     2281 agtctattta gctccagcag ccagctcttt cctccttctc ggctccgcta taatagatct
     2341 gacatcatgc cctccggccg cagtaggtta ttggaagatt tcaggaacaa ccgcttcccc
     2401 aaccttcagc tcagagactt aattggacat atagtcgagt tttctcaaga ccagcatggt
     2461 tccagattca tacagcaaaa gctagagaga gctactccag ctgagcgaca aatagtattt
     2521 aatgaaattc tacaggcagc ctatcaatta atgacagatg tttttggaaa ctatgttata
     2581 cagaagtttt ttgagtttgg aagtttggat cagaaattag ccctggctac tcgtattcgt
     2641 ggtcatgttc taccattagc cttgcagatg tatggctgcc gtgttattca aaaggcgtta
     2701 gaatctattt cttctgatca gcagagtgaa atggttaagg aactagatgg ccatgtactt
     2761 aaatgtgtga aagatcaaaa tggaaaccat gttgtacaga aatgcattga atgtgttcag
     2821 ccacagtcac tgcagttcat catcgatgct ttcaaaggac aagtatttgt gctttcaacc
     2881 catccttatg gctgcagagt cattcagcgt atcttagagc actgcacggc agagcagacc
     2941 ttacccatct tagaagaact tcaccaacac acagaacagt tggtacagga tcagtatggc
     3001 aattatgtta ttcagcatgt actggaacat ggtcgacctg aagacaagag caaaattgtt
     3061 tccgaaatca gaggaaaggt cttagccctg agtcaacaca aatttgccag caatgtagta
     3121 gaaaagtgtg ttactcatgc ctcccgtgct gagagagctt tactgattga tgaggtctgc
     3181 tgtcagaatg atggtcctca cagtgcctta tacaccatga tgaaggatca gtatgccaac
     3241 tatgtggttc agaagatgat tgatatggct gagcctgcgc agagaaagat aatcatgcac
     3301 aagattcgac ctcatattac tactcttcgc aaatacacat atgggaagca tatactggcc
     3361 aagttggaaa aatactatct gaaaaacagc ccagatctag ggccaattgg aggaccacca
     3421 aatgggatgc tgtaaggaca aacgcggagc tgctttaacc atgttcaaag agtgggcttt
     3481 ctttgtgaat tatcaaaaca caattcaact atgaatcttt gatttttttt taaagcaaaa
     3541 ctatttattg actttcttca tccatttgta aatttttaag gttcttgtgt atatttaggg
     3601 ggtggggggg tgaattataa attatattca accctgagtg gagacctatc agattggatt
     3661 tctggtgaag cacagaatgc ctgtatatga tgtaactgtg tccccccctc ccgcccccca
     3721 ccaaaaaaaa aaaaggaaaa aaagttgtca catattttgt aaatttatac cttgtaaagt
     3781 cacaaaaaat agtttttaaa gggaaaagta caatatttta ataaactggc ttgcagtctg
     3841 gtaggtctac aaccccatag cacaacaggt ttatagagat gtatatagaa ttatagttct
     3901 tatttttttc ctttgtgtga agccttttat aacagattaa caatcaactg cataaatatt
     3961 attaatattt taaaaagaaa gttaagttgt attttggtaa ttcacaaact atcatgcaaa
     4021 gaaaaactca gaaagacaga aaagaataaa atgatttgag ataacattat ttacagtgga
     4081 tgtggtttta ataaaattac tgccctataa tgtccctgac aatgtacaga attgtatata
     4141 cttataattg taatgagtta gaaacaaaat catggtggca cttccatcca gttaaaactg
     4201 cactgaatga aaagttaaat ctcacactaa cttttcaatt tggtttgcaa caagaatgga
     4261 tggagattta tattttcttc taattataga attactaggc tgttgaggaa gtgttggttt
     4321 gttctgcgtt ttcatagtag atacataaag taggaccccg atagaggtga gagaggccac
     4381 tgagaagtga agtgatagct cagcatttac gcatgattgc atattcagag agcttttttc
     4441 ccttcctata aataaacgca ttgtgtgtgt aggggtagat gtaagtgtag aatttgaaag
     4501 caaatcttgt ttcagtgttt gttatggaag ccttgctaat ttcatagtga cgttccttgg
     4561 ttgtacaggt gtacatttgt aaaccttcat gctgtaaatg gaatctgttt tctctcttcg
     4621 ggaaacattt gcatcttagt gtacatttcc atccctgccc cttaacctgg caatatagtg
     4681 ttgtataatg taaatttatt tctccaaatc gagagtgatt tttacaaatt ttttatcttt
     4741 atatggtttc agaagtatga accagctttc ttgttattat tgtgggagac attttgtttt
     4801 ataacatagt tgttgactct gttaataacg gacgtgctag gatctggatc actttcaatt
     4861 gaagtcaggg tattgtgcat aatgaacaga aagtattgga ctgagattga ttaccacaga
     4921 ggccaatgct ttttccatct ttaaatgtga tgtgactttt tttctttgta cagaagagta
     4981 ctgtattttt gaatagccta ctcccaagta agagcaaatc tgtatgataa catttttttc
     5041 ctctggacat aagtcattat aacagtaaca tgatgtacat ttacaagcgg ccttatgtac
     5101 atttcccaac aatcttttta aggcaaaatt gtgaccatat gtgtataatt aaaatcgttt
     5161 ttaatccttt gcctatgaaa atattttgga aaaaattgct ttgtatattc agtttctgaa
     5221 agataaagaa agtgctttgt attttgttga agtcagtatt ttatataaac tttatgttga
     5281 cccacttatg ttcagtgcta aaaactgaaa ggaacatgct atttctggca gatgaatagt
     5341 agaagggatt tttttattcg aggtctgcag aagaaaaaca atcagagcac aatattgagt
     5401 ataaagattc tatccttgtg ttactcatat catgaacctt aaaatttggc atgaagtttg
     5461 gggtgccagg agtctttaac attgatgctg agttactcta gctttcaaac acaacattcc
     5521 ttggtgaggc cactaagtct ggccatgttt caccattctc agtcacactg cttcatcagc
     5581 aggtccctgt cacagtagca gtattctcgc tcatgtagaa taattctctt ctcccctttt
     5641 ctactttgtt ttgccagtct cagagtatct tggccctggg ttgaatttaa cttcctctca
     5701 tcagcccata gtttcggatc aaagttgttg gtgttagaga ttagtaacag ctcttcctgc
     5761 agaaatgcag cccattctgg tttgtcctca cccttccctt tggatgcccc ggcagctgtc
     5821 actcggctca gaaaagtggc aagtcctaga gaaagttatt tggaactgaa tcctcctcta
     5881 gcagtcctga ttcatacttc ccagtgctga caacccagtc ctcctgatac ttttaggcat
     5941 attctagaaa aaacctgttc gtgtagaaaa tttatgatct gccagttttg gtgcccaccc
     6001 tgattccacc ttctagtatg ttggcatgga actttagaaa aatctgacaa tacagaataa
     6061 agacatctga ttgggcagaa tccacttagg ctttaattct tgagtagtct ctttttgatg
     6121 tagattgttt ctggaccagt ttgtctaagt cctggctctt attggctcaa atgaaatgtt
     6181 atcttcactt tgtatattat gtataaatta gaaaatgaaa cgtgtgaata acattgtatg
     6241 aaataaacct ggtcttgtgt ttttctctag ataaaatatc cctctatatc tctgtcaaaa
     6301 aaaaa
//
