LOCUS       NM_028364               1872 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus poly-U binding splicing factor 60 (Puf60), transcript
            variant 1, mRNA.
ACCESSION   NM_028364
VERSION     NM_028364.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Matsushita,K., Kitamura,K., Rahmutulla,B., Tanaka,N., Ishige,T.,
            Satoh,M., Hoshino,T., Miyagi,S., Mori,T., Itoga,S., Shimada,H.,
            Tomonaga,T., Kito,M., Nakajima-Takagi,Y., Kubo,S., Nakaseko,C.,
            Hatano,M., Miki,T., Matsuo,M., Fukuyo,M., Kaneda,A., Iwama,A. and
            Nomura,F.
  TITLE     Haploinsufficiency of the c-myc transcriptional repressor FIR, as a
            dominant negative-alternative splicing model, promoted
            p53-dependent T-cell acute lymphoblastic leukemia progression by
            activating Notch1
  JOURNAL   Oncotarget 6 (7), 5102-5117 (2015)
   PUBMED   25671302
REFERENCE   3  (bases 1 to 1872)
  AUTHORS   Rahmutulla,B., Matsushita,K., Satoh,M., Seimiya,M., Tsuchida,S.,
            Kubo,S., Shimada,H., Ohtsuka,M., Miyazaki,M. and Nomura,F.
  TITLE     Alternative splicing of FBP-interacting repressor coordinates
            c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular
            sensor for bleomycin-induced DNA damage pathway
  JOURNAL   Oncotarget 5 (9), 2404-2417 (2014)
   PUBMED   24811221
REFERENCE   4  (bases 1 to 1872)
  AUTHORS   Sansom,S.N., Griffiths,D.S., Faedo,A., Kleinjan,D.J., Ruan,Y.,
            Smith,J., van Heyningen,V., Rubenstein,J.L. and Livesey,F.J.
  TITLE     The level of the transcription factor Pax6 is essential for
            controlling the balance between neural stem cell self-renewal and
            neurogenesis
  JOURNAL   PLoS Genet 5 (6), e1000511 (2009)
   PUBMED   19521500
REFERENCE   5  (bases 1 to 1872)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116487.14.
            
            On Mar 25, 2022 this sequence version replaced NM_028364.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK149632.1, AK146534.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AC116487.14        16067-16129
            64-165              AC116487.14        19277-19378
            166-261             AC116487.14        21147-21242
            262-351             AC116487.14        21348-21437
            352-402             AC116487.14        22322-22372
            403-564             AC116487.14        24411-24572
            565-657             AC116487.14        24654-24746
            658-871             AC116487.14        25026-25239
            872-1062            AC116487.14        25323-25513
            1063-1198           AC116487.14        25662-25797
            1199-1434           AC116487.14        25995-26230
            1435-1872           AC116487.14        26352-26789
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 35.32 cM"
     gene            1..1872
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /note="poly-U binding splicing factor 60"
                     /db_xref="GeneID:67959"
                     /db_xref="MGI:MGI:1915209"
     exon            1..63
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1734
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /note="isoform a is encoded by transcript variant 1;
                     poly(U)-binding-splicing factor PUF60; 60 kDa
                     poly(U)-binding-splicing factor; fuse-binding
                     protein-interacting repressor"
                     /codon_start=1
                     /product="poly(U)-binding-splicing factor PUF60 isoform a"
                     /protein_id="NP_082640.2"
                     /db_xref="CCDS:CCDS37112.1"
                     /db_xref="GeneID:67959"
                     /db_xref="MGI:MGI:1915209"
                     /translation="MATATIALQVNGQQGGGSEPAAAAAAAAAAVVAAGDKWKPPQGT
                     ESIKMENGQSTGTKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTN
                     LQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPI
                     KSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQP
                     IIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGF
                     IEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAA
                     TAKITAQEAVAGAAVLGTLATPGLVSPALTLAQPLGALPQAVMAAQAPGVITGVTPAR
                     PPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGS
                     SARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQ
                     GEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQERFDNSDLSA"
     exon            64..165
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            166..261
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            262..351
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            352..402
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            403..564
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            565..657
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            658..871
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1062
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1198
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1434
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1872
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1851..1856
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /note="hexamer: CATAAA"
     polyA_site      1872
                     /gene="Puf60"
                     /gene_synonym="2410104I19Rik; 2810454F19Rik; SIAHBP1"
                     /note="major polyA site"
ORIGIN      
        1 gatgcttgtg gcgcgcgcgg cggccgcgac ggacgcaaga tggcgacggc gactatagct
       61 ctccaggtca atggccaaca aggaggaggg tcggagccag cagcagcggc tgcagcggcg
      121 gcggcggcag tggtggcagc aggagacaaa tggaaacctc cacagggcac agaatccatc
      181 aagatggaaa atggacaaag cacaggcacc aagctggggc tgcctcccct gacgcccgag
      241 cagcaggagg ccctccagaa ggccaagaaa tatgcaatgg agcagagcat caagagtgtg
      301 ctggtgaaac agaccatcgc gcaccagcag cagcagctca ccaacctgca gatggcagca
      361 gtgacaatgg gctttggaga tcctctctca cctttgcaat cgatggcagc tcaacggcag
      421 cgggcactgg ctatcatgtg ccgggtctat gtgggctcca tctactatga gctgggagaa
      481 gacactattc gccaggcctt tgctcccttt ggccccatca agagcattga tatgtcctgg
      541 gactccgtta ccatgaagca taagggcttt gccttcgtgg agtacgaggt cccagaagct
      601 gcgcagctcg ctttggagca gatgaactct gtgatgcttg ggggcaggaa catcaaggtg
      661 ggaagaccca gcaacattgg acaagcccaa cccatcatag atcagctggc tgaggaagct
      721 agggctttca accgaatata tgtggcctct gtacatcaag acctgtctga tgatgacatc
      781 aagagtgtat ttgaagcctt tggcaagatc aagtcttgta cgctggcccg ggaccccaca
      841 actggcaagc acaagggcta tggttttatc gaatatgaga aggcccagtc gtcccaggat
      901 gctgtgtcct ccatgaacct ctttgatctg ggtggccagt acttgagggt gggcaaggcc
      961 gtcacacccc ccatgcccct gctaacacct gccacgcctg gaggtctccc gcctgctgct
     1021 gctgtggccg cagctgcagc cacggccaag attacagctc aggaagcagt ggctggagca
     1081 gcagtgctgg gtactttagc cacaccagga ctagtgtccc cagcactgac actggcccag
     1141 cccttagggg ccctacccca ggctgtcatg gctgcccagg cccctggagt catcacaggt
     1201 gtgacaccag cccgccctcc tattccggtc accatcccct ctgtgggagt agtgaacccc
     1261 attctcgcca gcccgccaac gttgggtctg ttggagccca agaaggagaa ggaagaggag
     1321 gagctgtttc ctgagtcaga acggccagag atgttgagtg agcaggagca catgagcatc
     1381 tcgggcagca gtgctcgcca catggtcatg caaaagctac tcagaaaaca ggagtctaca
     1441 gtgatggttc tccgaaacat ggtggacccc aaggacatcg atgacgacct ggagggagag
     1501 gtgacagagg agtgtggcaa atttggtgct gtgaaccggg tcattatcta tcaagaaaaa
     1561 cagggcgaag aggaagatgc agaaatcatc gtcaagattt ttgtggaatt ctccatggcc
     1621 tcagagactc acaaggccat ccaggccctc aatgggcgct ggtttggtgg tcgcaaggtg
     1681 gtggctgaag tgtatgacca ggagcgtttt gataacagcg acctctccgc gtgactttgg
     1741 cctcatttcc tggacttgca cttgcccctt ccttcctttg ggttttgtaa agtgttaacc
     1801 gtggtgtgtc cctgggacca ggcactctgc ccaacccacc ctgtagtgta cataaagatg
     1861 cagataccac tg
//
