LOCUS       NM_138748               2577 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus protein phosphatase 2 protein activator (Ptpa),
            transcript variant 2, mRNA.
ACCESSION   NM_138748
VERSION     NM_138748.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Jayappa,K.D., Tran,B., Gordon,V.L., Morris,C., Saha,S.,
            Farrington,C.C., O'Connor,C.M., Zawacki,K.P., Isaac,K.M.,
            Kester,M., Bender,T.P., Williams,M.E., Portell,C.A., Weber,M.J. and
            Narla,G.
  TITLE     PP2A modulation overcomes multidrug resistance in chronic
            lymphocytic leukemia via mPTP-dependent apoptosis
  JOURNAL   J Clin Invest 133 (13), e155938 (2023)
   PUBMED   37166997
  REMARK    GeneRIF: PP2A modulation overcomes multidrug resistance in chronic
            lymphocytic leukemia via mPTP-dependent apoptosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2577)
  AUTHORS   Meeusen,B., Cortesi,E.E., Domenech Omella,J., Sablina,A.,
            Ventura,J.J. and Janssens,V.
  TITLE     PPP2R4 dysfunction promotes KRAS-mutant lung adenocarcinoma
            development and mediates opposite responses to MEK and mTOR
            inhibition
  JOURNAL   Cancer Lett 520, 57-67 (2021)
   PUBMED   34216687
REFERENCE   4  (bases 1 to 2577)
  AUTHORS   Wang,J., Lou,S.S., Wang,T., Wu,R.J., Li,G., Zhao,M., Lu,B.,
            Li,Y.Y., Zhang,J., Cheng,X., Shen,Y., Wang,X., Zhu,Z.C., Li,M.J.,
            Takumi,T., Yang,H., Yu,X., Liao,L. and Xiong,Z.Q.
  TITLE     UBE3A-mediated PTPA ubiquitination and degradation regulate PP2A
            activity and dendritic spine morphology
  JOURNAL   Proc Natl Acad Sci U S A 116 (25), 12500-12505 (2019)
   PUBMED   31160454
  REMARK    GeneRIF: results implicate a critical role of UBE3A-PTPA-PP2A
            signaling in the pathogenesis of UBE3A-related disorders and
            suggest that PP2A-based drugs could be potential therapeutic
            candidates for treatment of UBE3A-related disorders
REFERENCE   5  (bases 1 to 2577)
  AUTHORS   Sents,W., Meeusen,B., Kalev,P., Radaelli,E., Sagaert,X.,
            Miermans,E., Haesen,D., Lambrecht,C., Dewerchin,M., Carmeliet,P.,
            Westermarck,J., Sablina,A. and Janssens,V.
  TITLE     PP2A Inactivation Mediated by PPP2R4 Haploinsufficiency Promotes
            Cancer Development
  JOURNAL   Cancer Res 77 (24), 6825-6837 (2017)
   PUBMED   29046336
  REMARK    GeneRIF: Evidence for PPP2R4 as a haploinsufficient tumor
            suppressor gene, defining a high-penetrance genetic mechanism for
            PP2A inhibition in human cancer.
REFERENCE   6  (bases 1 to 2577)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
REFERENCE   7  (bases 1 to 2577)
  AUTHORS   Kim,T.S., Kim,H.D., Shin,H.S. and Kim,J.
  TITLE     Phosphorylation status of nuclear ribosomal protein S3 is
            reciprocally regulated by protein kinase C{delta} and protein
            phosphatase 2A
  JOURNAL   J Biol Chem 284 (32), 21201-21208 (2009)
   PUBMED   19458393
  REMARK    GeneRIF: oxidative stress regulates the phosphorylation status of
            nonribosomal rpS3 by both activating PKCdelta and blocking the PP2A
            interaction with rpS3
REFERENCE   8  (bases 1 to 2577)
  AUTHORS   Ortega-Gutierrez,S., Leung,D., Ficarro,S., Peters,E.C. and
            Cravatt,B.F.
  TITLE     Targeted disruption of the PME-1 gene causes loss of demethylated
            PP2A and perinatal lethality in mice
  JOURNAL   PLoS One 3 (7), e2486 (2008)
   PUBMED   18596935
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2577)
  AUTHORS   Azam,S., Drobetsky,E. and Ramotar,D.
  TITLE     Overexpression of the cis/trans isomerase PTPA triggers caspase
            3-dependent apoptosis
  JOURNAL   Apoptosis 12 (7), 1243-1255 (2007)
   PUBMED   17333320
  REMARK    GeneRIF: findings show that ectopic expression of the
            phosphotyrosyl phosphatase activator PTPA induces cell death in
            mammalian cells via a mechanism involving caspase-3-dependent
            apoptosis
REFERENCE   10 (bases 1 to 2577)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL954299.13 and AK049184.1.
            
            On Jul 23, 2009 this sequence version replaced NM_138748.4.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK031591.1, AK150029.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AL954299.13        10250-10259
            11-2573             AK049184.1         2-2564
            2574-2577           AL954299.13        42004-42007
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 21.71 cM"
     gene            1..2577
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /note="protein phosphatase 2 protein activator"
                     /db_xref="GeneID:110854"
                     /db_xref="MGI:MGI:1346006"
     exon            1..283
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     CDS             253..1224
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /EC_number="5.2.1.8"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serine/threonine-protein phosphatase 2A regulatory subunit
                     B'; serine/threonine-protein phosphatase 2A activator;
                     protein phosphatase 2A activator, regulatory subunit B;
                     protein phosphatase 2A, regulatory subunit B (PR 53);
                     phosphotyrosyl phosphatase activator;
                     serine/threonine-protein phosphatase 2A regulatory subunit
                     4"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 2A
                     activator isoform 2"
                     /protein_id="NP_620087.3"
                     /db_xref="CCDS:CCDS15883.1"
                     /db_xref="GeneID:110854"
                     /db_xref="MGI:MGI:1346006"
                     /translation="MAEGERQPPPDSSEETPPTTQNFIIPKKEIHTVPDMGKWKRSQA
                     YADYIGFILTLNEGVKGKKLTFDYKVSEAIEKLVALLDTLDRWIDETPPVDQPSRFGN
                     KAYRTWYAKLDQEAENLVATVVPTHLAAAVPEVAVYLKEAVGNSTRIDYGTGHEAAFA
                     AFLCCLCKIGVLRVDDQVAIVFKVFDRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFL
                     PFIWGSSQLIDHPHLEPRHFVDEKAVSENHKDYMFLQCILFITEMKTGPFAEHSNQLW
                     NISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG"
     exon            284..381
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            382..468
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            469..594
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            595..712
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            713..812
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            813..937
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1038
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1146
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..2577
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2551..2556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /note="hexamer: ATTAAA"
     polyA_site      2575
                     /gene="Ptpa"
                     /gene_synonym="2610042B21Rik; Ppp2r4; PR53"
                     /note="major polyA site"
ORIGIN      
        1 ggcgccccgc accaagatgg ccgctgcctc ggtcggcgcg gccgccgcga gacggccgtt
       61 ggtgggcatg ctccctgagt gcctcgcacc ggcatggctg ccgccttcgc gcgagtgact
      121 gtaattctcg gcttccggtc ctagccagtc agcgctcatt tccctgctgg aagcttggcg
      181 gagccgggga gagctgcggg gtgaacattg agagggaccg gcgagcatcc gctccggctc
      241 ctcacggaga agatggccga gggcgagcgg cagccgccgc cagattcttc agaagagacc
      301 cctccaacta ctcagaactt tatcattcca aaaaaggaga tccacacagt tccagatatg
      361 ggcaaatgga agcgctctca ggcatatgct gactacattg gcttcatcct taccctcaat
      421 gaaggtgtga aggggaagaa gctgaccttc gactacaaag tctctgaggc catcgagaag
      481 ctggtggcac ttcttgatac gctggatagg tggattgatg aaaccccgcc agtggaccag
      541 ccttcccggt ttgggaacaa agcctacaga acctggtatg ccaaacttga tcaggaagca
      601 gaaaacttgg tggccacagt ggtccccacc cacctggctg ctgctgtgcc tgaagtggca
      661 gtttacctga aggaggctgt ggggaactcc acacgaattg actatggcac agggcatgag
      721 gctgcctttg ctgctttcct ctgttgtctc tgcaagattg gtgtactccg ggtggacgac
      781 caggtggcta ttgtcttcaa ggtgtttgat aggtatcttg aggttatgcg gaagttgcag
      841 aagacataca ggatggagcc tgcaggcagc cagggcgtat ggggtctgga tgacttccag
      901 ttcctgccct tcatctgggg cagctcacag ctcatagacc acccccacct ggagcccaga
      961 catttcgtgg atgagaaggc ggtgagcgag aaccacaagg actacatgtt tctccagtgc
     1021 atcctgttca tcactgagat gaagactggc ccctttgcgg aacactccaa ccagctgtgg
     1081 aacatcagtg ctgtcccctc ctggtctaaa gtgaaccagg gcctcattcg aatgtataag
     1141 gcagagtgcc tggagaagtt ccctgtgatc cagcacttca agttcgggag cctgctgccc
     1201 atccatcccg tcacatcagg ctaggagagg ctgaactacc agagccaccc tggccaggtt
     1261 cctgtgctct ctgtgaccca gcacccctcc cgcccacctt ctgttctttc tgtttgatga
     1321 gaggctgttt actgagatgg gtgctaagca gggcctgagt tgggggaatt gaccacagtg
     1381 tgcccagttt ttgactgtcc tccgtctggt ggccgagtaa gaagagaggg gcctggacgt
     1441 ggtccccggc cctgcccatc cctgtgctcc tgcttcctcc cacggtcagg cccatgtgga
     1501 ggagacaagg acaccctgct cttgtcattg ctcccccttt cctccccatg atgtaatggt
     1561 tccctcatta agagcaggag ttccgtgcct ccagggccgg ctggtgggtt ccccgctagc
     1621 tgtgttccac tgggtatctg ctatggagct gaagggtgag gctgtcatca gctgttggga
     1681 gggctggggt tgggggaggt tggtttgtag gggccatgga tgatgaggct ggtcttctca
     1741 aggtaccatc ctaggccctg ggggacctgt gtccatcctc atgctggctt tgttccctgg
     1801 cccttggctt tgctaggttt cctgactgtt aacataacac tccccttcct ggggatagcc
     1861 tagctatgga agctgccctg ggctcctggg aaagcccatt gcttgggtga gatctgactt
     1921 tccagagctg tcaagcaggg agaggaagag actccgggag gcagtaggac tgttgtgtct
     1981 gtggagaacc tagttttctc cagtcagtct caggcctgct gattctgctg cctctgcctg
     2041 ctgagtgtct gaagcttccc ccgaggcctt ggcttgctgc tccgctccct tgggtcggtt
     2101 tgtggctcgt gtgggagagc aaagaaggta gcagccccct cctcccactt ccctctgtga
     2161 gatgagggaa gtgctggcct gggcctaggc atagactcag aatgggtagg gctgctaagg
     2221 ggacctagcc acagtcacca gttttctctc agcgtgtggc tgaactggaa cctgcaggct
     2281 ttcctgtccc acttgggcct gcccttcttc actgcccatc ctgtgtttgt gcccgcttag
     2341 ctttgtggag tggacactga gaagcacaac tcaggggctt ggccaggaat taactatggt
     2401 ctgggcagag cggggattga agtcatcaga gtcacaggca gttccccagg aaaagcccag
     2461 tgccagcttc cttccagggc caggccactg ccttcctggc tcccttccct gtccctgcgt
     2521 ccccagccct gcgccttcct tgctatttgg attaaagcct ctgttttaca cctgtcc
//
