LOCUS       NM_011198               4384 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus prostaglandin-endoperoxide synthase 2 (Ptgs2), mRNA.
ACCESSION   NM_011198
VERSION     NM_011198.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ide,H., Miike,K., Ohmori,T., Maruyama,K., Izumi,Y., Tanigawa,S. and
            Nishinakamura,R.
  TITLE     Mouse embryonic kidney transplantation identifies maturation
            defects in the medulla
  JOURNAL   Sci Rep 14 (1), 30293 (2024)
   PUBMED   39639083
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Zhu,J. and Liang,J.
  TITLE     The role of COX2 deficiency attenuates cardiac damage in acute
            myocardial infarction
  JOURNAL   BMC Cardiovasc Disord 24 (1), 623 (2024)
   PUBMED   39511505
  REMARK    GeneRIF: The role of COX2 deficiency attenuates cardiac damage in
            acute myocardial infarction.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4384)
  AUTHORS   Aikawa,S., Matsuo,M., Akaeda,S., Sugimoto,Y., Arita,M., Isobe,Y.,
            Sugiura,Y., Taira,S., Maeda,R., Shimizu-Hirota,R., Takeda,N.,
            Hiratsuka,D., He,X., Ishizawa,C., Iida,R., Fukui,Y., Hiraoka,T.,
            Harada,M., Wada-Hiraike,O., Osuga,Y. and Hirota,Y.
  TITLE     Spatiotemporally distinct roles of cyclooxygenase-1 and
            cyclooxygenase-2 at fetomaternal interface in mice
  JOURNAL   JCI Insight 9 (19), e181865 (2024)
   PUBMED   39377223
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4384)
  AUTHORS   Sakurai,J., Oka,S., Higuchi,Y., Ohsawa,S. and Fujimori,T.
  TITLE     Effects of blastocyst elongation and implantation chamber formation
            on the alignment of the embryonic axis and uterine axis in mice
  JOURNAL   Front Cell Dev Biol 12, 1421222 (2024)
   PUBMED   38946796
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4384)
  AUTHORS   Kiefer,J.R., Pawlitz,J.L., Moreland,K.T., Stegeman,R.A., Hood,W.F.,
            Gierse,J.K., Stevens,A.M., Goodwin,D.C., Rowlinson,S.W.,
            Marnett,L.J., Stallings,W.C. and Kurumbail,R.G.
  TITLE     Structural insights into the stereochemistry of the cyclooxygenase
            reaction
  JOURNAL   Nature 405 (6782), 97-101 (2000)
   PUBMED   10811226
REFERENCE   6  (bases 1 to 4384)
  AUTHORS   Wen,P.Z., Warden,C., Fletcher,B.S., Kujubu,D.A., Herschman,H.R. and
            Lusis,A.J.
  TITLE     Chromosomal organization of the inducible and constitutive
            prostaglandin synthase/cyclooxygenase genes in mouse
  JOURNAL   Genomics 15 (2), 458-460 (1993)
   PUBMED   8449520
REFERENCE   7  (bases 1 to 4384)
  AUTHORS   Ryseck,R.P., Raynoschek,C., Macdonald-Bravo,H., Dorfman,K.,
            Mattei,M.G. and Bravo,R.
  TITLE     Identification of an immediate early gene, pghs-B, whose protein
            product has prostaglandin synthase/cyclooxygenase activity
  JOURNAL   Cell Growth Differ 3 (7), 443-450 (1992)
   PUBMED   1419907
REFERENCE   8  (bases 1 to 4384)
  AUTHORS   O'Banion,M.K., Winn,V.D. and Young,D.A.
  TITLE     cDNA cloning and functional activity of a glucocorticoid-regulated
            inflammatory cyclooxygenase
  JOURNAL   Proc Natl Acad Sci U S A 89 (11), 4888-4892 (1992)
   PUBMED   1594589
REFERENCE   9  (bases 1 to 4384)
  AUTHORS   Fletcher,B.S., Kujubu,D.A., Perrin,D.M. and Herschman,H.R.
  TITLE     Structure of the mitogen-inducible TIS10 gene and demonstration
            that the TIS10-encoded protein is a functional prostaglandin G/H
            synthase
  JOURNAL   J Biol Chem 267 (7), 4338-4344 (1992)
   PUBMED   1339449
REFERENCE   10 (bases 1 to 4384)
  AUTHORS   Kujubu,D.A., Fletcher,B.S., Varnum,B.C., Lim,R.W. and
            Herschman,H.R.
  TITLE     TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3
            cells, encodes a novel prostaglandin synthase/cyclooxygenase
            homologue
  JOURNAL   J Biol Chem 266 (20), 12866-12872 (1991)
   PUBMED   1712772
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114655.12.
            
            On Oct 12, 2022 this sequence version replaced NM_011198.4.
            
            Summary: This gene encodes an enzyme that is a member of the
            prostaglandin G/H synthase family. The encoded protein converts
            arachidonic acid to prostaglandin endoperoxide H2 which is a key
            enzymatic step in prostaglandin biosynthesis. This gene is the
            inducible prostaglandin G/H synthase family member that is
            upregulated during inflammation. Aberrant regulation of this gene
            is associated with cancer progression in several tissues and an
            increased risk of cardiovascular events. This gene is the target of
            many non-steroidal anti-inflammatory drugs. [provided by RefSeq,
            Sep 2015].
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK149820.1, AK144956.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-176               AC114655.12        77794-77969
            177-293             AC114655.12        78668-78784
            294-437             AC114655.12        78907-79050
            438-581             AC114655.12        79685-79828
            582-763             AC114655.12        80290-80471
            764-847             AC114655.12        81369-81452
            848-1094            AC114655.12        81563-81809
            1095-1381           AC114655.12        81977-82263
            1382-1529           AC114655.12        82636-82783
            1530-4384           AC114655.12        83067-85921
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 63.84 cM"
     gene            1..4384
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /note="prostaglandin-endoperoxide synthase 2"
                     /db_xref="GeneID:19225"
                     /db_xref="MGI:MGI:97798"
     exon            1..176
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..1939
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /EC_number="1.14.99.1"
                     /note="prostaglandin G/H synthase 2; PGH synthase 2;
                     prostaglandin H2 synthase 2; glucocorticoid-regulated
                     inflammatory cyclooxygenase; macrophage
                     activation-associated marker protein P71/73;
                     cyclooxygenase-2"
                     /codon_start=1
                     /product="prostaglandin G/H synthase 2 precursor"
                     /protein_id="NP_035328.2"
                     /db_xref="CCDS:CCDS15353.1"
                     /db_xref="GeneID:19225"
                     /db_xref="MGI:MGI:97798"
                     /translation="MLFRAVLLCAALGLSQAANPCCSNPCQNRGECMSTGFDQYKCDC
                     TRTGFYGENCTTPEFLTRIKLLLKPTPNTVHYILTHFKGVWNIVNNIPFLRSLIMKYV
                     LTSRSYLIDSPPTYNVHYGYKSWEAFSNLSYYTRALPPVADDCPTPMGVKGNKELPDS
                     KEVLEKVLLRREFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPGFTRGLGHGVDLNH
                     IYGETLDRQHKLRLFKDGKLKYQVIGGEVYPPTVKDTQVEMIYPPHIPENLQFAVGQE
                     VFGLVPGLMMYATIWLREHNRVCDILKQEHPEWGDEQLFQTSRLILIGETIKIVIEDY
                     VQHLSGYHFKLKFDPELLFNQQFQYQNRIASEFNTLYHWHPLLPDTFNIEDQEYSFKQ
                     FLYNNSILLEHGLTQFVESFTRQIAGRVAGGRNVPIAVQAVAKASIDQSREMKYQSLN
                     EYRKRFSLKPYTSFEELTGEKEMAAELKALYSDIDVMELYPALLVEKPRPDAIFGETM
                     VELGAPFSLKGLMGNPICSPQYWKPSTFGGEVGFKIINTASIQSLICNNVKGCPFTSF
                     NVQDPQPTKTATINASASHSRLDDINPTVLIKRRSTEL"
     exon            177..293
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     exon            294..437
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     exon            438..581
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     exon            582..763
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     exon            764..847
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     exon            848..1094
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1381
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1529
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..4384
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4299..4304
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /note="hexamer: AATAAA"
     polyA_site      4316
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /note="major polyA site"
     regulatory      4362..4367
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /note="hexamer: AATAAA"
     polyA_site      4384
                     /gene="Ptgs2"
                     /gene_synonym="Cox-2; COX2; gripghs; PES-2; PGHS-2; Pghs2;
                     PHS II; PHS-2; TIS10"
                     /note="major polyA site"
ORIGIN      
        1 agttgtcaaa ctgcgagcta agagcttcag gagtcagtca ggactctgct cacgaaggaa
       61 ctcagcactg catcctgcca gctccaccgc caccactact gccacctccg ctgccacctc
      121 tgcgatgctc ttccgagctg tgctgctctg cgctgccctg gggctcagcc aggcagcaaa
      181 tccttgctgt tccaatccat gtcaaaaccg tggggaatgt atgagcacag gatttgacca
      241 gtataagtgt gactgtaccc ggactggatt ctatggtgaa aactgtacta cacctgaatt
      301 tctgacaaga atcaaattac tgctgaagcc caccccaaac acagtgcact acatcctgac
      361 ccacttcaag ggagtctgga acattgtgaa caacatcccc ttcctgcgaa gtttaatcat
      421 gaaatatgtg ctgacatcca gatcatattt gattgacagt ccacctactt acaatgtgca
      481 ctatggttac aaaagctggg aagccttctc caacctctcc tactacacca gggcccttcc
      541 tcccgtagca gatgactgcc caactcccat gggtgtgaag ggaaataagg agcttcctga
      601 ttcaaaagaa gtgctggaaa aggttcttct acggagagag ttcatccctg acccccaagg
      661 ctcaaatatg atgtttgcat tctttgccca gcacttcacc catcagtttt tcaagacaga
      721 tcataagcga ggacctgggt tcacccgagg actgggccat ggagtggact taaatcacat
      781 ttatggtgaa actctggaca gacaacataa actgcgcctt ttcaaggatg gaaaattgaa
      841 atatcaggtc attggtggag aggtgtatcc ccccacagtc aaagacactc aggtagagat
      901 gatctaccct cctcacatcc ctgagaacct gcagtttgct gtggggcagg aagtctttgg
      961 tctggtgcct ggtctgatga tgtatgccac catctggctt cgggagcaca acagagtgtg
     1021 cgacatactc aagcaggagc atcctgagtg gggtgatgag caactattcc aaaccagcag
     1081 actcatactc ataggagaga ctatcaagat agtgatcgaa gactacgtgc aacacctgag
     1141 cggttaccac ttcaaactca agtttgaccc agagctcctt ttcaaccagc agttccagta
     1201 tcagaaccgc attgcctctg aattcaacac actctatcac tggcaccccc tgctgcccga
     1261 caccttcaac attgaagacc aggagtacag cttcaaacag tttctctaca acaactccat
     1321 cctcctggaa catggactca ctcagtttgt tgagtcattc accagacaga ttgctggccg
     1381 ggttgctggg ggaagaaatg tgccaattgc tgtacaagca gtggcaaagg cctccattga
     1441 ccagagcaga gagatgaaat accagtctct caatgagtac cgcaaacgct tctccctgaa
     1501 gccgtacaca tcatttgaag aacttacagg agagaaggaa atggctgcag aattgaaagc
     1561 cctctacagt gacatcgatg tcatggaact gtaccctgcc ctgctggtgg aaaaacctcg
     1621 tccagatgct atctttgggg agaccatggt agagcttgga gcaccattct ccttgaaagg
     1681 acttatggga aatcccatct gttctcctca atactggaag ccgagcacct ttggaggcga
     1741 agtgggtttt aagatcatca atactgcctc aattcagtct ctcatctgca ataatgtgaa
     1801 ggggtgtccc ttcacttctt tcaatgtgca agatccacag cctaccaaaa cagccaccat
     1861 caatgcaagt gcctcccact ccagactaga tgacattaac cctacagtac taatcaaaag
     1921 gcgttcaact gagctgtaaa agtctactga ccatatttat ttatttatgt gaagaattta
     1981 atttaattat ttaatattta tactgaattt tttttcatgt aacatcttcc ataacagaag
     2041 gcaatgttct tgaacaatgt tacatttgtg aagattccct ccggtgtttg tcctttaaat
     2101 atgtgttacc tgaaactgaa aggaaatcag cattcattcc tctacataag ccagtgagaa
     2161 gggaaatgaa ttttgatatc tttatacttg aatttcagat catgattagc ttaacaagaa
     2221 ccaaggaaaa atgtatgaat atgtgagtgt tgttacaaga tgaaaaatgc tgcaggtatc
     2281 aacactgttg gttacaactg tgtcttcttt actatgatag gagcatgtaa tgtggaattc
     2341 ttcttaaatc ttgcatatct ttatctcatc aaacaaaggg gtccaagttc agttttaaat
     2401 aagcatttaa ggcagatact gacaacaatc tcatttttta aaatgttgtc ttgagacaaa
     2461 taatttgaaa tttctaaatt gggagtttga atcacttttg aaagctctta ctttcttaag
     2521 ctgtcaggtt tgtaccgaca tggagtaaac agctatcata aacgtaaatc tccaaaacta
     2581 gtagaaatta tgtcatgatt gatggttaag ataccatgtc agggattgtc ttttcttaga
     2641 agtagtgaaa gctacttact atgacaatca gaccttcctt gtatgtcaaa atgctggtgt
     2701 ggaaggtggt ggagcccgtg ctgctctgtc ttaactatga gtgtgagctt taaagctcgt
     2761 tgatgagtgg tagccagcaa agcctagagc aacaaaagct tctacaaagg aactaaccaa
     2821 gaacaaagaa gggttcccaa ttaaagatca cattcagggt taaacttcca aaggagacat
     2881 cctgatcctg gttttgtgct ggcctggtac tcagtaggtt tttgctgtga ggttaaagac
     2941 ttgccaggct gaacttcgaa acagtttttc tgttgcacag tatgatgtaa cagtccatct
     3001 ctcaatgcaa taggtatcag tggcctcgtg agcttcttca caatattgat atgtcttcca
     3061 gcccattgaa cctggactgc agaaggcccc atgtcatgtg tgagctcagc ctggatgcca
     3121 gcattgctgc tcctcttagt tccgtttctc gtggtcactt tactacgaga aacgctgatt
     3181 gggttttcgt agctgtgttc caggttttta gtatcagaac tattctttct ttaacctcta
     3241 ttcatatttt ctctacttga agttttacat tcaggaaaac ctcagctcag gactactatg
     3301 tacctcccct ttggagggaa aaattatttt aggtaaaagg caaaaatttt ttaaaaatat
     3361 ttttatttat aattatatgg aagggcccta ccaagatgct agaaatatag ggagttcctg
     3421 acaagaaatt tccattctta ttctgaagaa ttgctttctt acttaaaaac aaagacagtt
     3481 tgtgagtagt tctgggcaat agggataaat ataaaacaat aatgatgatc attttctaca
     3541 tctcattatc agctgaggta ctgtatatta ctgaatttat tgaagatagt tatgtctttt
     3601 agacattgtt gttataaact atgtttaagc ctactacaag tgtttctttt tgcattatgt
     3661 tggaattgat gtaccttttt tatgattacc tctctgaact atggtgtgaa caatcaaaca
     3721 aaatgatgag attaacgttc atggataaat tctaagaaaa ctagtgtatt tttttgaaaa
     3781 gtttgaagtt agaacttagg ctgttggaat ttacgcataa agcagactgc atagatccaa
     3841 tattgactga cccaagcatg ttataaagac tgacatttta gacattttga aggccctgta
     3901 agtgtttatt aattagttag aacttaattg attaaaaaat atatccaaag cactataggc
     3961 attagaattc gtgcatcaag aaatgatgac aaataatact gttatttata taaataacta
     4021 aaaagggtgt ctaatgaaga aatatatttt attacaaaga aattataaaa cattttgaag
     4081 attatatgct ttaaaagttt aagatgaaaa aataatcaac cttagaaaaa atgtataaaa
     4141 atatataaat tgttaatgtc attgattaaa aaaaaaaaaa actagccaac atgatttttg
     4201 tgaatatcaa cctgctgatt gaacctggga attcaggtta agtatgtgac tgctacagtg
     4261 cctcagagaa atttgtattt aaattaactt atgtaaaaaa taaatcttag agcaaatgct
     4321 tgtttatttt tatacttatt taagaattga aaacttctga aaataaagtt tgattgtttc
     4381 ttta
//
