LOCUS       NM_008968               1837 bp    mRNA    linear   ROD 24-DEC-2022
DEFINITION  Mus musculus prostaglandin I2 (prostacyclin) synthase (Ptgis),
            mRNA.
ACCESSION   NM_008968
VERSION     NM_008968.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ochiai T, Sasaki Y, Yokoyama C, Kuwata H and Hara S.
  TITLE     Absence of prostacyclin greatly relieves cyclophosphamide-induced
            cystitis and bladder pain in mice
  JOURNAL   FASEB J 35 (10), e21952 (2021)
   PUBMED   34555210
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Sasaki Y, Kuwata H, Akatsu M, Yamakawa Y, Ochiai T, Yoda E,
            Nakatani Y, Yokoyama C and Hara S.
  TITLE     Involvement of prostacyclin synthase in high-fat-diet-induced
            obesity
  JOURNAL   Prostaglandins Other Lipid Mediat 153, 106523 (2021)
   PUBMED   33383181
REFERENCE   3  (bases 1 to 1837)
  AUTHORS   Ochiai T, Sasaki Y, Kuwata H, Nakatani Y, Yokoyama C and Hara S.
  TITLE     Coordinated action of microsomal prostaglandin E synthase-1 and
            prostacyclin synthase on contact hypersensitivity
  JOURNAL   Biochem Biophys Res Commun 546, 124-129 (2021)
   PUBMED   33582554
  REMARK    GeneRIF: Coordinated action of microsomal prostaglandin E
            synthase-1 and prostacyclin synthase on contact hypersensitivity.
REFERENCE   4  (bases 1 to 1837)
  AUTHORS   Sasaki Y, Kamiyama S, Kamiyama A, Matsumoto K, Akatsu M, Nakatani
            Y, Kuwata H, Ishikawa Y, Ishii T, Yokoyama C and Hara S.
  TITLE     Genetic-deletion of Cyclooxygenase-2 Downstream Prostacyclin
            Synthase Suppresses Inflammatory Reactions but Facilitates
            Carcinogenesis, unlike Deletion of Microsomal Prostaglandin E
            Synthase-1
  JOURNAL   Sci Rep 5, 17376 (2015)
   PUBMED   26611322
  REMARK    GeneRIF: Prostacyclin synthase and prostaglandin E synthase-1
            cooperatively exacerbate inflammatory reactions but have opposing
            effects on carcinogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1837)
  AUTHORS   Umansky KB, Gruenbaum-Cohen Y, Tsoory M, Feldmesser E, Goldenberg
            D, Brenner O and Groner Y.
  TITLE     Runx1 Transcription Factor Is Required for Myoblasts Proliferation
            during Muscle Regeneration
  JOURNAL   PLoS Genet 11 (8), e1005457 (2015)
   PUBMED   26275053
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1837)
  AUTHORS   Yokoyama C, Yabuki T, Shimonishi M, Wada M, Hatae T, Ohkawara S,
            Takeda J, Kinoshita T, Okabe M and Tanabe T.
  TITLE     Prostacyclin-deficient mice develop ischemic renal disorders,
            including nephrosclerosis and renal infarction
  JOURNAL   Circulation 106 (18), 2397-2403 (2002)
   PUBMED   12403673
REFERENCE   7  (bases 1 to 1837)
  AUTHORS   Pradono P, Tazawa R, Maemondo M, Tanaka M, Usui K, Saijo Y,
            Hagiwara K and Nukiwa T.
  TITLE     Gene transfer of thromboxane A(2) synthase and prostaglandin I(2)
            synthase antithetically altered tumor angiogenesis and tumor growth
  JOURNAL   Cancer Res 62 (1), 63-66 (2002)
   PUBMED   11782360
  REMARK    GeneRIF: Gene transfer of thromboxane A(2) synthase and
            prostaglandin I(2) synthase antithetically altered tumor
            angiogenesis and tumor growth.
REFERENCE   8  (bases 1 to 1837)
  AUTHORS   Ohkawara S, Shimonishi M, Tanabe T and Yokoyama C.
  TITLE     Cloning and characterization of 5'-flanking region of mouse
            prostacyclin synthase gene
  JOURNAL   Biochim Biophys Acta 1494 (1-2), 155-161 (2000)
   PUBMED   11072079
REFERENCE   9  (bases 1 to 1837)
  AUTHORS   Lim H, Gupta RA, Ma WG, Paria BC, Moller DE, Morrow JD, DuBois RN,
            Trzaskos JM and Dey SK.
  TITLE     Cyclo-oxygenase-2-derived prostacyclin mediates embryo implantation
            in the mouse via PPARdelta
  JOURNAL   Genes Dev 13 (12), 1561-1574 (1999)
   PUBMED   10385625
REFERENCE   10 (bases 1 to 1837)
  AUTHORS   Kuwamoto S, Inoue H, Tone Y, Izumi Y and Tanabe T.
  TITLE     Inverse gene expression of prostacyclin and thromboxane synthases
            in resident and activated peritoneal macrophages
  JOURNAL   FEBS Lett 409 (2), 242-246 (1997)
   PUBMED   9202154
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591854.18, AK076585.1 and CD742059.1.
            
            [WARNING] On May 5, 2023 this sequence was replaced by NM_008968.5.
            
            On Apr 24, 2018 this sequence version replaced NM_008968.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062151.1, AK076585.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                AL591854.18        117543-117610       c
            69-1819             AK076585.1         2-1752
            1820-1837           CD742059.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2"
     gene            1..1837
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /note="prostaglandin I2 (prostacyclin) synthase"
                     /db_xref="GeneID:19223"
                     /db_xref="MGI:MGI:1097156"
     exon            1..262
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..1691
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /EC_number="5.3.99.4"
                     /EC_number="4.2.1.152"
                     /note="prostacyclin synthase; hydroperoxy icosatetraenoate
                     dehydratase"
                     /codon_start=1
                     /product="prostacyclin synthase precursor"
                     /protein_id="NP_032994.1"
                     /db_xref="CCDS:CCDS17097.1"
                     /db_xref="GeneID:19223"
                     /db_xref="MGI:MGI:1097156"
                     /translation="MSWAALLGLLAVLLLLLLLLSRRRARRPGEPPLDLGSIPWLGHA
                     LEFGRDAASFLTRMKEKHGDIFTVLVGGRYVTVLLDPHSYDTVVWELRTRLDFHPYAI
                     FLMERIFDLQLPNFNPSEEKARMKPTLMHRDLQALTEAMYTNLRTVLLGDSTEAGSGW
                     QETGLLEFSYNALLSAGYLTLYGVEASPRTHESQAQDRVHSADVFHTFRQLDLLLPKL
                     ARGSLSAGDKDHACSVKNRLWKLLSPARLASRADRSSWLESYLRHLEEMGVSEEMQAR
                     ALVLQLWATQGNMGPTAFWLLLFLLKNPEALAAVRAELKHTVWQAEQPVSQMTTLPQK
                     ILDSMPVLDSVLNETLRLTAAPFITREVMADLALPMADGREFSLRRGDRLLLFPFLSP
                     QKDPEIYTEPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNQCLGKSYAIN
                     SIKQFVVLLLTHFDLELGSEDTEVPEFDLSRYGFGLMQPEEDVPIRYRARL"
     exon            263..386
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     exon            387..565
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     exon            566..709
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     exon            710..861
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1043
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1212
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1394
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1546
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1821
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaccccctct cctctcctct gcggcagccc ggccatccct cccttcccgc tccttcctct
       61 ccatccacgt tttccagtcg gacccctaga gtcggggcct ttggggctgg ctgggttgag
      121 aatcctgcgg tccggtgttg gtggcggtga cttgttgcca ccctgcagcc gcacaccctc
      181 gagccatgtc ctgggctgct ctgctcggcc tcctggccgt gctattgctg ttgctgctgc
      241 tgctgagccg ccggcgcgcg cggagacctg gtgagcctcc gttggacctg ggcagcatcc
      301 cctggctggg ccatgccttg gagtttggga gagatgctgc cagcttcctt accaggatga
      361 aggaaaagca cggtgacata tttactgtgc tggtgggggg cagatacgtc actgttctcc
      421 tggacccaca ctcttacgac acagtggtgt gggagctgcg tacacggctg gacttccatc
      481 cctatgccat cttcctcatg gagaggattt ttgatctgca gcttccaaat ttcaacccca
      541 gtgaggagaa ggccaggatg aagccgacgc tcatgcacag agacctgcag gcgctcacag
      601 aagccatgta taccaacctt cgcacggtcc tgctgggtga ctccacagaa gcaggcagtg
      661 gctggcagga gacaggtctc cttgagttct cctacaacgc cctgctcagc gccggctacc
      721 tgaccctata tggagtggag gcctcaccac gcacccatga gagtcaggcc caagatcgtg
      781 tccactcagc ggatgttttc cataccttcc gccagctgga tctgctgctc cccaaactgg
      841 ctcgaggctc cttgtcagcg ggggataaag accatgcatg cagtgtcaaa aaccgcctgt
      901 ggaagctgct gtccccagcc aggctggcct ccagggctga ccggagcagc tggctggaga
      961 gttatctgag acatcttgag gagatggggg tgtcagagga gatgcaggcc cgggctctgg
     1021 tgttgcagct ctgggccaca caggggaata tgggtcccac ggccttctgg ctccttcttt
     1081 tcctcctcaa gaatccggaa gccctcgccg ctgtccgtgc agagctgaag cacacggtct
     1141 ggcaagcaga gcagcctgtt tcacagatga ccacactccc acagaagatt ctagacagca
     1201 tgcctgtgct agacagtgtg ctcaatgaga cactccggct cacggctgcc cccttcatca
     1261 cccgtgaggt catggcagac ctggccttgc ctatggcaga cgggagggaa ttctctcttc
     1321 gacgtggtga ccgccttctc ctctttccct tcctgagtcc ccagaaggac ccagaaatct
     1381 acacagagcc tgaggtgttt aaatacaacc gattcttgaa cccagatgga tctgaaaaga
     1441 aagattttta caaagatggg aaacggctga agaattacaa catgccatgg ggcgcagggc
     1501 acaaccagtg cctggggaag agttatgcca tcaacagcat caaacaattt gtcgtcctac
     1561 tgctgactca tttcgacctg gagctgggca gcgaggacac agaggttcct gagtttgacc
     1621 tcagcagata tggcttcggt ctgatgcagc cagaggaaga cgtgcccatc cggtaccgtg
     1681 ccaggctgtg acctatggag cagactcacc tggggccttc tgacttcctg ttgttcctct
     1741 agaatgctat gtccagcgga ggggacactg aggacagtga gagcaggaaa agctaataaa
     1801 agctctgtat ctcgctgtga aaaaaaaaaa aaaaaaa
//
