LOCUS       NP_032994                501 aa            linear   ROD 15-OCT-2024
DEFINITION  prostacyclin synthase isoform a [Mus musculus].
ACCESSION   NP_032994
VERSION     NP_032994.1
DBSOURCE    REFSEQ: accession NM_008968.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 501)
  AUTHORS   Ge,J., Zhou,Y., Li,H., Zeng,R., Xie,K., Leng,J., Chen,X., Yu,G.,
            Shi,X., Xu,Y., He,D., Guo,P., Zhou,Y., Luo,H., Luo,W. and Liu,B.
  TITLE     Prostacyclin Synthase Deficiency Leads to Exacerbation or
            Occurrence of Endothelium-Dependent Contraction and Causes
            Cardiovascular Disorders Mainly via the Non-TxA2 Prostanoids/TP
            Axis
  JOURNAL   Circ Res 135 (6), e133-e149 (2024)
   PUBMED   39082135
  REMARK    GeneRIF: Prostacyclin Synthase Deficiency Leads to Exacerbation or
            Occurrence of Endothelium-Dependent Contraction and Causes
            Cardiovascular Disorders Mainly via the Non-TxA2 Prostanoids/TP
            Axis.
REFERENCE   2  (residues 1 to 501)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 501)
  AUTHORS   Wang,B., Ge,S., Wang,Z., Wang,W., Wang,Y., Leng,H. and Ma,X.
  TITLE     Analysis and experimental validation of fatty acid
            metabolism-related genes prostacyclin synthase (PTGIS) in
            endometrial cancer
  JOURNAL   Aging (Albany NY) 15 (19), 10322-10346 (2023)
   PUBMED   37796199
  REMARK    GeneRIF: Analysis and experimental validation of fatty acid
            metabolism-related genes prostacyclin synthase (PTGIS) in
            endometrial cancer.
REFERENCE   4  (residues 1 to 501)
  AUTHORS   Ochiai,T., Sasaki,Y., Yokoyama,C., Kuwata,H. and Hara,S.
  TITLE     Absence of prostacyclin greatly relieves cyclophosphamide-induced
            cystitis and bladder pain in mice
  JOURNAL   FASEB J 35 (10), e21952 (2021)
   PUBMED   34555210
REFERENCE   5  (residues 1 to 501)
  AUTHORS   Ochiai,T., Sasaki,Y., Kuwata,H., Nakatani,Y., Yokoyama,C. and
            Hara,S.
  TITLE     Coordinated action of microsomal prostaglandin E synthase-1 and
            prostacyclin synthase on contact hypersensitivity
  JOURNAL   Biochem Biophys Res Commun 546, 124-129 (2021)
   PUBMED   33582554
  REMARK    GeneRIF: Coordinated action of microsomal prostaglandin E
            synthase-1 and prostacyclin synthase on contact hypersensitivity.
REFERENCE   6  (residues 1 to 501)
  AUTHORS   Yokoyama,C., Yabuki,T., Shimonishi,M., Wada,M., Hatae,T.,
            Ohkawara,S., Takeda,J., Kinoshita,T., Okabe,M. and Tanabe,T.
  TITLE     Prostacyclin-deficient mice develop ischemic renal disorders,
            including nephrosclerosis and renal infarction
  JOURNAL   Circulation 106 (18), 2397-2403 (2002)
   PUBMED   12403673
REFERENCE   7  (residues 1 to 501)
  AUTHORS   Pradono,P., Tazawa,R., Maemondo,M., Tanaka,M., Usui,K., Saijo,Y.,
            Hagiwara,K. and Nukiwa,T.
  TITLE     Gene transfer of thromboxane A(2) synthase and prostaglandin I(2)
            synthase antithetically altered tumor angiogenesis and tumor growth
  JOURNAL   Cancer Res 62 (1), 63-66 (2002)
   PUBMED   11782360
  REMARK    GeneRIF: Gene transfer of thromboxane A(2) synthase and
            prostaglandin I(2) synthase antithetically altered tumor
            angiogenesis and tumor growth.
REFERENCE   8  (residues 1 to 501)
  AUTHORS   Ohkawara,S., Shimonishi,M., Tanabe,T. and Yokoyama,C.
  TITLE     Cloning and characterization of 5'-flanking region of mouse
            prostacyclin synthase gene
  JOURNAL   Biochim Biophys Acta 1494 (1-2), 155-161 (2000)
   PUBMED   11072079
REFERENCE   9  (residues 1 to 501)
  AUTHORS   Lim,H., Gupta,R.A., Ma,W.G., Paria,B.C., Moller,D.E., Morrow,J.D.,
            DuBois,R.N., Trzaskos,J.M. and Dey,S.K.
  TITLE     Cyclo-oxygenase-2-derived prostacyclin mediates embryo implantation
            in the mouse via PPARdelta
  JOURNAL   Genes Dev 13 (12), 1561-1574 (1999)
   PUBMED   10385625
REFERENCE   10 (residues 1 to 501)
  AUTHORS   Kuwamoto,S., Inoue,H., Tone,Y., Izumi,Y. and Tanabe,T.
  TITLE     Inverse gene expression of prostacyclin and thromboxane synthases
            in resident and activated peritoneal macrophages
  JOURNAL   FEBS Lett 409 (2), 242-246 (1997)
   PUBMED   9202154
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591854.18.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062151.1, AK076585.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 87.22 cM"
     Protein         1..501
                     /product="prostacyclin synthase isoform a"
                     /EC_number="5.3.99.4"
                     /EC_number="4.2.1.152"
                     /note="prostacyclin synthase; hydroperoxy icosatetraenoate
                     dehydratase"
                     /calculated_mol_wt=56916
     Site            1..21
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O35074.1)"
     Region          53..494
                     /region_name="PGIS_CYP8A1"
                     /note="prostacyclin Synthase, also called cytochrome P450
                     family 8, subfamily A, polypeptide 1; cd20634"
                     /db_xref="CDD:410727"
     Site            order(97,100..101,104,283..285,288,356..360,483)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410727"
     Site            order(122,129,181,284..285,288..289,292,350,356,434..436,
                     440,442..444,447..448,452,486)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410727"
     CDS             1..501
                     /gene="Ptgis"
                     /gene_synonym="Cyp8; Cyp8a1; Pgis"
                     /coded_by="NM_008968.5:152..1657"
                     /note="isoform a is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS17097.1"
                     /db_xref="GeneID:19223"
                     /db_xref="MGI:MGI:1097156"
ORIGIN      
        1 mswaallgll avllllllll srrrarrpge ppldlgsipw lghalefgrd aasfltrmke
       61 khgdiftvlv ggryvtvlld phsydtvvwe lrtrldfhpy aiflmerifd lqlpnfnpse
      121 ekarmkptlm hrdlqaltea mytnlrtvll gdsteagsgw qetgllefsy nallsagylt
      181 lygveasprt hesqaqdrvh sadvfhtfrq ldlllpklar gslsagdkdh acsvknrlwk
      241 llsparlasr adrsswlesy lrhleemgvs eemqaralvl qlwatqgnmg ptafwlllfl
      301 lknpealaav raelkhtvwq aeqpvsqmtt lpqkildsmp vldsvlnetl rltaapfitr
      361 evmadlalpm adgrefslrr gdrlllfpfl spqkdpeiyt epevfkynrf lnpdgsekkd
      421 fykdgkrlkn ynmpwgaghn qclgksyain sikqfvvlll thfdlelgse dtevpefdls
      481 rygfglmqpe edvpiryrar l
//
