LOCUS       NM_008965               3264 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus prostaglandin E receptor 4 (subtype EP4) (Ptger4),
            transcript variant 2, mRNA.
ACCESSION   NM_008965
VERSION     NM_008965.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ansari,S.S., Dillard,M.E., Zhang,Y., Austria,M.A., Boatwright,N.,
            Shelton,E.L., Stewart,D.P., Johnson,A., Wang,C.E., Young,B.M.,
            Rankovic,Z., Hansen,B.S., Pruett-Miller,S.M., Carisey,A.F.,
            Schuetz,J.D., Robinson,C.G. and Ogden,S.K.
  TITLE     Sonic Hedgehog activates prostaglandin signaling to stabilize
            primary cilium length
  JOURNAL   J Cell Biol 223 (9) (2024)
   PUBMED   38856684
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Taketomi,Y., Higashi,T., Kano,K., Miki,Y., Mochizuki,C.,
            Toyoshima,S., Okayama,Y., Nishito,Y., Nakae,S., Tanaka,S.,
            Tokuoka,S.M., Oda,Y., Shichino,S., Ueha,S., Matsushima,K.,
            Akahoshi,N., Ishii,S., Chun,J., Aoki,J. and Murakami,M.
  TITLE     Lipid-orchestrated paracrine circuit coordinates mast cell
            maturation and anaphylaxis through functional interaction with
            fibroblasts
  JOURNAL   Immunity 57 (8), 1828-1847 (2024)
   PUBMED   39002541
REFERENCE   3  (bases 1 to 3264)
  AUTHORS   Lacher,S.B., Dorr,J., de Almeida,G.P., Honninger,J., Bayerl,F.,
            Hirschberger,A., Pedde,A.M., Meiser,P., Ramsauer,L., Rudolph,T.J.,
            Spranger,N., Morotti,M., Grimm,A.J., Jarosch,S., Oner,A.,
            Gregor,L., Lesch,S., Michaelides,S., Fertig,L., Briukhovetska,D.,
            Majed,L., Stock,S., Busch,D.H., Buchholz,V.R., Knolle,P.A.,
            Zehn,D., Dangaj Laniti,D., Kobold,S. and Bottcher,J.P.
  TITLE     PGE2 limits effector expansion of tumour-infiltrating stem-like
            CD8+ T cells
  JOURNAL   Nature 629 (8011), 417-425 (2024)
   PUBMED   38658748
REFERENCE   4  (bases 1 to 3264)
  AUTHORS   Yarboro,M.T., Boatwright,N., Sekulich,D.C., Hooper,C.W., Wong,T.,
            Poole,S.D., Berger,C.D., Brown,A.J., Jetter,C.S., Sucre,J.M.S.,
            Shelton,E.L. and Reese,J.
  TITLE     A novel role for PGE2-EP4 in the developmental programming of the
            mouse ductus arteriosus: consequences for vessel maturation and
            function
  JOURNAL   Am J Physiol Heart Circ Physiol 325 (4), H687-H701 (2023)
   PUBMED   37566109
REFERENCE   5  (bases 1 to 3264)
  AUTHORS   Meiser,P., Knolle,M.A., Hirschberger,A., de Almeida,G.P.,
            Bayerl,F., Lacher,S., Pedde,A.M., Flommersfeld,S., Honninger,J.,
            Stark,L., Stogbauer,F., Anton,M., Wirth,M., Wohlleber,D.,
            Steiger,K., Buchholz,V.R., Wollenberg,B., Zielinski,C.E.,
            Braren,R., Rueckert,D., Knolle,P.A., Kaissis,G. and Bottcher,J.P.
  TITLE     A distinct stimulatory cDC1 subpopulation amplifies CD8+ T cell
            responses in tumors for protective anti-cancer immunity
  JOURNAL   Cancer Cell 41 (8), 1498-1515 (2023)
   PUBMED   37451271
REFERENCE   6  (bases 1 to 3264)
  AUTHORS   Nishigaki,N., Negishi,M., Honda,A., Sugimoto,Y., Namba,T.,
            Narumiya,S. and Ichikawa,A.
  TITLE     Identification of prostaglandin E receptor 'EP2' cloned from
            mastocytoma cells EP4 subtype
  JOURNAL   FEBS Lett 364 (3), 339-341 (1995)
   PUBMED   7758593
REFERENCE   7  (bases 1 to 3264)
  AUTHORS   Taketo,M., Rochelle,J.M., Sugimoto,Y., Namba,T., Honda,A.,
            Negishi,M., Ichikawa,A., Narumiya,S. and Seldin,M.F.
  TITLE     Mapping of the genes encoding mouse thromboxane A2 receptor and
            prostaglandin E receptor subtypes EP2 and EP3
  JOURNAL   Genomics 19 (3), 585-588 (1994)
   PUBMED   7910583
REFERENCE   8  (bases 1 to 3264)
  AUTHORS   Honda,A., Sugimoto,Y., Namba,T., Watabe,A., Irie,A., Negishi,M.,
            Narumiya,S. and Ichikawa,A.
  TITLE     Cloning and expression of a cDNA for mouse prostaglandin E receptor
            EP2 subtype
  JOURNAL   J Biol Chem 268 (11), 7759-7762 (1993)
   PUBMED   8385118
REFERENCE   9  (bases 1 to 3264)
  AUTHORS   Idell,S., James,K.K., Levin,E.G., Schwartz,B.S., Manchanda,N.,
            Maunder,R.J., Martin,T.R., McLarty,J. and Fair,D.S.
  TITLE     Local abnormalities in coagulation and fibrinolytic pathways
            predispose to alveolar fibrin deposition in the adult respiratory
            distress syndrome
  JOURNAL   J Clin Invest 84 (2), 695-705 (1989)
   PUBMED   2788176
REFERENCE   10 (bases 1 to 3264)
  AUTHORS   Fuller,J.L., Brady-Wood,S. and Elias,M.F.
  TITLE     Effects of retinal degeneration and brain size upon spatial
            reversal learning in mice
  JOURNAL   Percept Mot Skills 36 (3), 947-950 (1973)
   PUBMED   4704351
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC135079.9.
            
            On Oct 2, 2013 this sequence version replaced NM_008965.1.
            
            Transcript Variant: This variant (2) encodes the longer isoform
            (2).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156428.1, AK145045.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-505               AC135079.9         26204-26708
            506-1440            AC135079.9         27196-28130
            1441-3264           AC135079.9         35169-36992
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 1.99 cM"
     gene            1..3264
                     /gene="Ptger4"
                     /gene_synonym="EP4; Ptgerep4"
                     /note="prostaglandin E receptor 4 (subtype EP4)"
                     /db_xref="GeneID:19219"
                     /db_xref="MGI:MGI:104311"
     exon            1..505
                     /gene="Ptger4"
                     /gene_synonym="EP4; Ptgerep4"
                     /inference="alignment:Splign:2.1.0"
     CDS             490..2031
                     /gene="Ptger4"
                     /gene_synonym="EP4; Ptgerep4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     prostaglandin E2 receptor EP4 subtype; prostanoid EP4
                     receptor; PGE2 receptor EP4 subtype"
                     /codon_start=1
                     /product="prostaglandin E2 receptor EP4 subtype isoform 2"
                     /protein_id="NP_032991.1"
                     /db_xref="CCDS:CCDS27365.1"
                     /db_xref="GeneID:19219"
                     /db_xref="MGI:MGI:104311"
                     /translation="MAEVGGTIPRSNRELQRCVLLTTTIMSIPGVNASFSSTPERLNS
                     PVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTI
                     ATYMKGQWPGDQALCDYSTFILLFFGLSGLSIICAMSIERYLAINHAYFYSHYVDKRL
                     AGLTLFAIYASNVLFCALPNMGLGRSERQYPGTWCFIDWTTNVTAYAAFSYMYAGFSS
                     FLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQHHAAAAAAVASVACRGHAGASPAL
                     QRLSDFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFINQLYQPNVVKDI
                     SRNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSGRDSSAQHCS
                     ESRRTSSAMSGHSRSFLARELKEISSTSQTLLYLPDLTESSLGGRNLLPGSHGMGLTQ
                     ADTTSLRTLRISETSDSSQGQDSESVLLVDEVSGSHREEPASKGNSLQVTFPSETLKL
                     SEKCI"
     exon            506..1440
                     /gene="Ptger4"
                     /gene_synonym="EP4; Ptgerep4"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..3264
                     /gene="Ptger4"
                     /gene_synonym="EP4; Ptgerep4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3241..3246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ptger4"
                     /gene_synonym="EP4; Ptgerep4"
                     /note="hexamer: AATAAA"
     polyA_site      3264
                     /gene="Ptger4"
                     /gene_synonym="EP4; Ptgerep4"
                     /note="major polyA site"
ORIGIN      
        1 cagaggctgg agacgccgag agcgaagctc gaaacccggc agcgggagag gaagatgaac
       61 ggcctcaggt cagagcccca ggcccagtgg accccgtgcc gcccccagag cccaaggagc
      121 ggcctcagcg gcagccgcct tctccggtag ctgtccgttc agcccgctgc tggggaggag
      181 ggagcccgga cctacgctct gttccacctg cacgcgtggc ggcgacacac ctggacgctg
      241 acctcccgca ctagacctgc gcgggccctg caaagctgag actcgccttt gaaaggagga
      301 ggcgttagta aatcaaacaa gaaaccccag actggtcttc accgacccat cccgctagcc
      361 tctctggctt tccaagcttt tttgaaagca agatactctg acctcagttc cggaaagttg
      421 gcagccaccg agccccggtt ccgagacagc aaaagcttga caagttccgc actgcgtggg
      481 aagagactga tggctgaggt tggaggtacc attcctagat cgaaccgtga gctccaacgc
      541 tgtgtgttac taaccaccac catcatgtcc atccccggag tcaacgcgtc cttctcctcc
      601 actccggaga ggctgaacag cccggtgacc attcccgcag tgatgttcat cttcggggtg
      661 gtgggcaacc tggtggccat cgtagtattg tgcaagtcgc gcaaggagca gaaagagacg
      721 accttttaca ctctagtatg tgggctggct gtcactgacc ttctgggcac cttgttggta
      781 agcccggtga ccatcgccac atacatgaag ggccagtggc ccggagacca ggcactgtgt
      841 gactatagca ccttcatcct acttttcttc ggtctgtcgg gtctcagcat catctgtgcc
      901 atgagcatcg agcgctacct ggccatcaac cacgcctact tctacagcca ctacgtggac
      961 aagcggctgg ccggcctcac actcttcgcc atctatgcat ctaacgtgct gttctgcgcg
     1021 ctgcccaaca tgggcctggg cagatccgag cggcagtacc cgggcacctg gtgcttcatc
     1081 gactggacca ccaacgtaac ggcctacgcc gccttctctt acatgtacgc cggcttcagc
     1141 tccttcctca tccttgccac cgtgctctgc aacgtgctgg tgtgcggcgc gctgctccgc
     1201 atgcaccgcc agttcatgcg ccgcacctcg ttgggcacgg agcagcacca tgcggctgcc
     1261 gccgccgcgg tagcttcggt ggcctgtcgg ggccacgctg gggcctcccc agccctgcag
     1321 cgcctcagcg actttcgccg ccgcaggagt ttccggcgca tcgcgggtgc ggagatccag
     1381 atggtcatct tactcatcgc cacctctctg gtggtgctca tctgctccat tccgctcgtg
     1441 gtgcgagtgt tcattaacca gttatatcag ccaaacgtgg tgaaagacat cagcagaaac
     1501 ccagatttgc aggccatcag gattgcttct gtgaacccca tcctggaccc ctggatttac
     1561 atccttcttc ggaagactgt gctcagtaaa gccatagaga agatcaagtg cctcttctgc
     1621 cgcattggcg gttccggcag agacagctcg gcccagcact gctcagagag tcggaggaca
     1681 tcttccgcca tgtccggcca ctctcgctcc ttcctcgccc gggagttaaa ggagatcagc
     1741 agcacgtccc agaccctcct gtacctgcca gacctgactg aaagcagcct cggaggcagg
     1801 aatttgcttc caggttcgca tggcatgggc ctgacccaag cagacaccac ctcgctgaga
     1861 actttgcgaa tttccgagac ctcagactcc tcccagggcc aggactctga gagtgtcctg
     1921 ttggtggatg aggttagtgg gagccacaga gaggagcctg cctctaaagg aaactctctg
     1981 caagtcacat tccccagtga aactctgaaa ttatctgaaa aatgtatata gtagctaaag
     2041 ggggaatctt ataaaatcct gtgcaataga catacatagc tgtactcaga agggctgtct
     2101 tcatctggac tcccactaga gaacaggcga gctcctgagg gctctccaag gctgcagact
     2161 gaggtccttg agtgcccagg cttgaagcac attggctgtc attctgatgt gactcgagat
     2221 tgcagttgca acttggcagc ttttttctac tggacaggaa gatggcagaa gctacgctat
     2281 tgtcatagca aaagagcttt ctatttggca cataccaggg gtccagctac tggaagggct
     2341 ctaccccaaa ctctgaggac taccttacag ctgacttaag tgtctcacta aagcatgaaa
     2401 tgtgaatttt tattgttgga aatataattt aaggtattta tgttcttctc tgtgagaagg
     2461 tttattgtta atacaaggta taaaaaacac atgatatgcc ctctcctgcc aatataacca
     2521 gctaatattg tcgatgttat tttttttttt ccataaacaa gttcaggcca aagtgttgaa
     2581 aacagagtga aactaatatc tataaaatag atataaattt ttaaaatagt ttagtatcat
     2641 caaagaaaaa ataagtagta tttaagatgt gaaaaatgaa caacctaaaa tatattttcc
     2701 aagctatata taataatgaa aaataaaaac attacattta tttatccaga aaactgtgat
     2761 tttaggatta cctaacattg ctggtaaata ttttcaacct ttgtccccac aaattggtat
     2821 ttttaaagat gtaacatact ttttttttta ggtctctagc tacccacaag ggaagtgtat
     2881 agcataaaaa gtctaagtag catatcctgt cctgaaagtg tgtgtagttt tacttgttca
     2941 acagaatgtc cgacagcatc ctttaaaact taaagagaaa gacaactttg cagaaagaga
     3001 cacagctggc tctctgcagt ggtgtcctct ctgttgcgtg tgtcgagaga cactgacagt
     3061 ttcgtgtaac tcccaggaat actgtacctg tgtttggagg agctttctca gagaaatagc
     3121 aagcatgtcc tgttgcttaa cgtgtccttg tgaattgctt ggttttgatt aaagtctgag
     3181 cctgatactg atatgatttt aaagcgtagc tgaaccgact gaaactatta tggagattac
     3241 aataaataat tgcattcaat ctga
//
