LOCUS       NP_032991                513 aa            linear   ROD 12-JUN-2025
DEFINITION  prostaglandin E2 receptor EP4 subtype isoform 2 [Mus musculus].
ACCESSION   NP_032991
VERSION     NP_032991.1
DBSOURCE    REFSEQ: accession NM_008965.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 513)
  AUTHORS   Goepp,M., Milburn,J.V., Zhang,B., Dong,Y., Tyrrell,V., Zheng,X.,
            Marshall,J.M., Bolsega,S., Basic,M., Glendinning,L., Ho,G.T.,
            Satsangi,J., Breyer,R.M., Narumiya,S., McSorley,H.J.,
            Schwarze,J.K.J., Anderson,C.J., Dockrell,D.H., Rossi,A.G.,
            Bleich,A., Lucas,C.D., O'Donnell,V.B., Mole,D., Arends,M.J.,
            Zhou,Y. and Yao,C.
  TITLE     Age-related impairment of intestinal inflammation resolution
            through an eicosanoid-immune-microbiota axis
  JOURNAL   Cell Host Microbe 33 (5), 671-687 (2025)
   PUBMED   40373750
REFERENCE   2  (residues 1 to 513)
  AUTHORS   Ansari,S.S., Dillard,M.E., Zhang,Y., Austria,M.A., Boatwright,N.,
            Shelton,E.L., Stewart,D.P., Johnson,A., Wang,C.E., Young,B.M.,
            Rankovic,Z., Hansen,B.S., Pruett-Miller,S.M., Carisey,A.F.,
            Schuetz,J.D., Robinson,C.G. and Ogden,S.K.
  TITLE     Sonic Hedgehog activates prostaglandin signaling to stabilize
            primary cilium length
  JOURNAL   J Cell Biol 223 (9) (2024)
   PUBMED   38856684
REFERENCE   3  (residues 1 to 513)
  AUTHORS   Taketomi,Y., Higashi,T., Kano,K., Miki,Y., Mochizuki,C.,
            Toyoshima,S., Okayama,Y., Nishito,Y., Nakae,S., Tanaka,S.,
            Tokuoka,S.M., Oda,Y., Shichino,S., Ueha,S., Matsushima,K.,
            Akahoshi,N., Ishii,S., Chun,J., Aoki,J. and Murakami,M.
  TITLE     Lipid-orchestrated paracrine circuit coordinates mast cell
            maturation and anaphylaxis through functional interaction with
            fibroblasts
  JOURNAL   Immunity 57 (8), 1828-1847 (2024)
   PUBMED   39002541
REFERENCE   4  (residues 1 to 513)
  AUTHORS   Lacher,S.B., Dorr,J., de Almeida,G.P., Honninger,J., Bayerl,F.,
            Hirschberger,A., Pedde,A.M., Meiser,P., Ramsauer,L., Rudolph,T.J.,
            Spranger,N., Morotti,M., Grimm,A.J., Jarosch,S., Oner,A.,
            Gregor,L., Lesch,S., Michaelides,S., Fertig,L., Briukhovetska,D.,
            Majed,L., Stock,S., Busch,D.H., Buchholz,V.R., Knolle,P.A.,
            Zehn,D., Dangaj Laniti,D., Kobold,S. and Bottcher,J.P.
  TITLE     PGE2 limits effector expansion of tumour-infiltrating stem-like
            CD8+ T cells
  JOURNAL   Nature 629 (8011), 417-425 (2024)
   PUBMED   38658748
REFERENCE   5  (residues 1 to 513)
  AUTHORS   Yarboro,M.T., Boatwright,N., Sekulich,D.C., Hooper,C.W., Wong,T.,
            Poole,S.D., Berger,C.D., Brown,A.J., Jetter,C.S., Sucre,J.M.S.,
            Shelton,E.L. and Reese,J.
  TITLE     A novel role for PGE2-EP4 in the developmental programming of the
            mouse ductus arteriosus: consequences for vessel maturation and
            function
  JOURNAL   Am J Physiol Heart Circ Physiol 325 (4), H687-H701 (2023)
   PUBMED   37566109
REFERENCE   6  (residues 1 to 513)
  AUTHORS   Nishigaki,N., Negishi,M., Honda,A., Sugimoto,Y., Namba,T.,
            Narumiya,S. and Ichikawa,A.
  TITLE     Identification of prostaglandin E receptor 'EP2' cloned from
            mastocytoma cells EP4 subtype
  JOURNAL   FEBS Lett 364 (3), 339-341 (1995)
   PUBMED   7758593
REFERENCE   7  (residues 1 to 513)
  AUTHORS   Taketo,M., Rochelle,J.M., Sugimoto,Y., Namba,T., Honda,A.,
            Negishi,M., Ichikawa,A., Narumiya,S. and Seldin,M.F.
  TITLE     Mapping of the genes encoding mouse thromboxane A2 receptor and
            prostaglandin E receptor subtypes EP2 and EP3
  JOURNAL   Genomics 19 (3), 585-588 (1994)
   PUBMED   7910583
REFERENCE   8  (residues 1 to 513)
  AUTHORS   Honda,A., Sugimoto,Y., Namba,T., Watabe,A., Irie,A., Negishi,M.,
            Narumiya,S. and Ichikawa,A.
  TITLE     Cloning and expression of a cDNA for mouse prostaglandin E receptor
            EP2 subtype
  JOURNAL   J Biol Chem 268 (11), 7759-7762 (1993)
   PUBMED   8385118
REFERENCE   9  (residues 1 to 513)
  AUTHORS   Idell,S., James,K.K., Levin,E.G., Schwartz,B.S., Manchanda,N.,
            Maunder,R.J., Martin,T.R., McLarty,J. and Fair,D.S.
  TITLE     Local abnormalities in coagulation and fibrinolytic pathways
            predispose to alveolar fibrin deposition in the adult respiratory
            distress syndrome
  JOURNAL   J Clin Invest 84 (2), 695-705 (1989)
   PUBMED   2788176
REFERENCE   10 (residues 1 to 513)
  AUTHORS   Fuller,J.L., Brady-Wood,S. and Elias,M.F.
  TITLE     Effects of retinal degeneration and brain size upon spatial
            reversal learning in mice
  JOURNAL   Percept Mot Skills 36 (3), 947-950 (1973)
   PUBMED   4704351
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC135079.9.
            
            Transcript Variant: This variant (2) encodes the longer isoform
            (2).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156428.1, AK145045.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 1.99 cM"
     Protein         1..513
                     /product="prostaglandin E2 receptor EP4 subtype isoform 2"
                     /note="prostaglandin E2 receptor EP4 subtype; prostanoid
                     EP4 receptor; PGE2 receptor EP4 subtype"
                     /calculated_mol_wt=56027
     Site            32
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P32240.1)"
     Region          43..369
                     /region_name="7tmA_PGE2_EP4"
                     /note="prostaglandin E2 receptor EP4 subtype, member of
                     the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15142"
                     /db_xref="CDD:320270"
     Region          44..70
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320270"
     Site            45..68
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P32240.1)"
     Region          79..105
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320270"
     Site            81..104
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P32240.1)"
     Site            order(101,104..105,117..122,124..125,128,173,175..179,205,
                     208..210,212..214,216..217,313,316..317,319..320,323,
                     335..336,338..340,343,346..347)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:320270"
     Region          117..147
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320270"
     Site            122..140
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P32240.1)"
     Region          159..181
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320270"
     Site            161..185
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P32240.1)"
     Region          205..234
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320270"
     Site            210..236
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P32240.1)"
     Region          293..323
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320270"
     Site            296..323
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P32240.1)"
     Region          336..361
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320270"
     Site            341..360
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P32240.1)"
     Region          383..403
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (P32240.1)"
     Site            402
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P35408; propagated from
                     UniProtKB/Swiss-Prot (P32240.1)"
     Site            405
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P35408; propagated from
                     UniProtKB/Swiss-Prot (P32240.1)"
     Site            407
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P35408; propagated from
                     UniProtKB/Swiss-Prot (P32240.1)"
     CDS             1..513
                     /gene="Ptger4"
                     /gene_synonym="EP4; Ptgerep4"
                     /coded_by="NM_008965.2:490..2031"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /db_xref="CCDS:CCDS27365.1"
                     /db_xref="GeneID:19219"
                     /db_xref="MGI:MGI:104311"
ORIGIN      
        1 maevggtipr snrelqrcvl ltttimsipg vnasfsstpe rlnspvtipa vmfifgvvgn
       61 lvaivvlcks rkeqkettfy tlvcglavtd llgtllvspv tiatymkgqw pgdqalcdys
      121 tfillffgls glsiicamsi erylainhay fyshyvdkrl agltlfaiya snvlfcalpn
      181 mglgrserqy pgtwcfidwt tnvtayaafs ymyagfssfl ilatvlcnvl vcgallrmhr
      241 qfmrrtslgt eqhhaaaaaa vasvacrgha gaspalqrls dfrrrrsfrr iagaeiqmvi
      301 lliatslvvl icsiplvvrv finqlyqpnv vkdisrnpdl qairiasvnp ildpwiyill
      361 rktvlskaie kikclfcrig gsgrdssaqh csesrrtssa msghsrsfla relkeissts
      421 qtllylpdlt esslggrnll pgshgmgltq adttslrtlr isetsdssqg qdsesvllvd
      481 evsgshreep askgnslqvt fpsetlklse kci
//
