LOCUS       NM_008960               8229 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus phosphatase and tensin homolog (Pten), mRNA.
ACCESSION   NM_008960
VERSION     NM_008960.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Zhang,F., Xiong,X., Li,Z., Wang,H., Wang,W., Zhao,Y. and Sun,Y.
  TITLE     RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and
            aggravates liver tumorigenesis
  JOURNAL   EMBO J 44 (4), 1185-1219 (2025)
   PUBMED   39762645
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Yanushko,D., German Falcon,B., El Bizri,R., Pervizou,D., Dolgos,R.,
            Keime,C., Ye,T., Thibault-Carpentier,C., Le Magnen,C., Henri,S.,
            Laverny,G. and Metzger,D.
  TITLE     p53-loss induced prostatic epithelial cell plasticity and invasion
            is driven by a crosstalk with the tumor microenvironment
  JOURNAL   Cell Death Dis 16 (1), 46 (2025)
   PUBMED   39865080
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8229)
  AUTHORS   Yonan,J.M., Chen,K.D., Baram,T.Z. and Steward,O.
  TITLE     PTEN deletion in the adult dentate gyrus induces epilepsy
  JOURNAL   Neurobiol Dis 203, 106736 (2024)
   PUBMED   39547478
REFERENCE   4  (bases 1 to 8229)
  AUTHORS   Portale,F., Carriero,R., Iovino,M., Kunderfranco,P., Pandini,M.,
            Marelli,G., Morina,N., Lazzeri,M., Casale,P., Colombo,P., De
            Simone,G., Camisaschi,C., Lugli,E., Basso,G., Cibella,J.,
            Marchini,S., Bordi,M., Meregalli,G., Garbin,A., Dambra,M.,
            Magrini,E., Rackwitz,W., Cecconi,F., Corbelli,A., Fiordaliso,F.,
            Eitler,J., Tonn,T. and Di Mitri,D.
  TITLE     C/EBPbeta-dependent autophagy inhibition hinders NK cell function
            in cancer
  JOURNAL   Nat Commun 15 (1), 10343 (2024)
   PUBMED   39609420
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8229)
  AUTHORS   Morel,K.L., German,B., Hamid,A.A., Nanda,J.S., Linder,S.,
            Bergman,A.M., van der Poel,H., Hofland,I., Bekers,E.M.,
            Trostel,S.Y., Burkhart,D.L., Wilkinson,S., Ku,A.T., Kim,M., Kim,J.,
            Ma,D., Plummer,J.T., You,S., Su,X.A., Zwart,W., Sowalsky,A.G.,
            Sweeney,C.J. and Ellis,L.
  TITLE     Low tristetraprolin expression activates phenotypic plasticity and
            primes transition to lethal prostate cancer in mice
  JOURNAL   J Clin Invest 135 (2), e175680 (2024)
   PUBMED   39560993
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8229)
  AUTHORS   Suzuki,A., de la Pompa,J.L., Stambolic,V., Elia,A.J., Sasaki,T.,
            del Barco Barrantes,I., Ho,A., Wakeham,A., Itie,A., Khoo,W.,
            Fukumoto,M. and Mak,T.W.
  TITLE     High cancer susceptibility and embryonic lethality associated with
            mutation of the PTEN tumor suppressor gene in mice
  JOURNAL   Curr Biol 8 (21), 1169-1178 (1998)
   PUBMED   9799734
REFERENCE   7  (bases 1 to 8229)
  AUTHORS   Stambolic,V., Suzuki,A., de la Pompa,J.L., Brothers,G.M.,
            Mirtsos,C., Sasaki,T., Ruland,J., Penninger,J.M., Siderovski,D.P.
            and Mak,T.W.
  TITLE     Negative regulation of PKB/Akt-dependent cell survival by the tumor
            suppressor PTEN
  JOURNAL   Cell 95 (1), 29-39 (1998)
   PUBMED   9778245
REFERENCE   8  (bases 1 to 8229)
  AUTHORS   Di Cristofano,A., Pesce,B., Cordon-Cardo,C. and Pandolfi,P.P.
  TITLE     Pten is essential for embryonic development and tumour suppression
  JOURNAL   Nat Genet 19 (4), 348-355 (1998)
   PUBMED   9697695
REFERENCE   9  (bases 1 to 8229)
  AUTHORS   Hansen,G.M. and Justice,M.J.
  TITLE     Pten, a candidate tumor suppressor gene, maps to mouse chromosome
            19
  JOURNAL   Mamm Genome 9 (1), 88-90 (1998)
   PUBMED   9434957
REFERENCE   10 (bases 1 to 8229)
  AUTHORS   Steck,P.A., Pershouse,M.A., Jasser,S.A., Yung,W.K., Lin,H.,
            Ligon,A.H., Langford,L.A., Baumgard,M.L., Hattier,T., Davis,T.,
            Frye,C., Hu,R., Swedlund,B., Teng,D.H. and Tavtigian,S.V.
  TITLE     Identification of a candidate tumour suppressor gene, MMAC1, at
            chromosome 10q23.3 that is mutated in multiple advanced cancers
  JOURNAL   Nat Genet 15 (4), 356-362 (1997)
   PUBMED   9090379
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U92437.1, AK138025.1,
            CK616227.1 and BU701098.1.
            
            On Feb 2, 2006 this sequence version replaced NM_008960.1.
            
            Summary: This gene encodes a phosphatase with dual activity against
            phospholipids and proteins, and acts as a tumor-suppressor. The
            protein contains four structural domains, a PIP2-binding domain, a
            catalytic tensin-type phosphatase domain, a C2 tensin-type domain
            and a PDZ-binding domain. The protein belongs to the protein
            tyrosine phosphatase family. Deletion of this gene in mice
            contribute to tumorigenesis in multiple tissues. [provided by
            RefSeq, Sep 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076980.1, AK088717.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2080              U92437.1           81-2160
            2081-2171           AK138025.1         390-480
            2172-2172           CK616227.1         5-5
            2173-8187           AK138025.1         481-6495
            8188-8229           BU701098.1         1-42                c
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 28.14 cM"
     gene            1..8229
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /note="phosphatase and tensin homolog"
                     /db_xref="GeneID:19211"
                     /db_xref="MGI:MGI:109583"
     exon            1..947
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             869..2080
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /EC_number="3.1.3.67"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /note="mutated in multiple advanced cancers 1;
                     mitochondrial PTENalpha; PTENepsilon; inositol
                     polyphosphate 3-phosphatase"
                     /codon_start=1
                     /product="phosphatidylinositol 3,4,5-trisphosphate
                     3-phosphatase and dual-specificity protein phosphatase
                     PTEN"
                     /protein_id="NP_032986.1"
                     /db_xref="CCDS:CCDS29753.1"
                     /db_xref="GeneID:19211"
                     /db_xref="MGI:MGI:109583"
                     /translation="MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEG
                     VYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIK
                     PFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRT
                     RDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVC
                     QLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVN
                     TFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKAN
                     RYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPF
                     DEDQHSQITKV"
     exon            948..1032
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1077
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1121
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1360
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1502
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1503..1669
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1894
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..8212
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2359..2364
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /note="hexamer: AATAAA"
     polyA_site      2385
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /note="major polyA site"
     regulatory      5356..5361
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /note="hexamer: ATTAAA"
     polyA_site      5379
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
     regulatory      8080..8085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /note="hexamer: ATTAAA"
     polyA_site      8105
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
     regulatory      8189..8194
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /note="hexamer: ATTAAA"
     polyA_site      8212
                     /gene="Pten"
                     /gene_synonym="2310035O07Rik; A130070J02Rik;
                     B430203M17Rik; MMAC1; PTENbeta; TEP1"
                     /note="major polyA site"
ORIGIN      
        1 gcggcaggat acgcgcttgg gcgtcgggac gcggctgcgc tcagctctct cctctcggaa
       61 gctgcagcca tgatggaagt ttgagagttg agccgctgtg aggccaggcc cggcgcaggc
      121 gagggagatg agagacggcg gcggccacgg cccagagccc ctctcagcgc ctgtgagcag
      181 ccgcgggggc agcgccctcg gggagccggc cgggcggcgg cggcggcagc ggcggcgggc
      241 ctcgcctcct cgtcgtctgt tctaaccggg cagcttctga gcagcttcgg agagagacgg
      301 tggaagaagc cgtgggctcg agcgggagcc ggcgcaggct cggcggctgc acctcccgct
      361 cctggagcgg gggggagaag cggcggcggc ggccgcggct ccggggaggg ggtcggagtc
      421 gcctgtcacc attgccaggg ctgggaacgc cggagagttg ctctctcccc ttctcctgcc
      481 tccaacacgg cggcggcggc ggcggcacgt ccagggaccc gggccggtgt taagcctccc
      541 gtccgccgcc gccgcacccc ccctggcccg ggctccggag gccgccggag gaggcagccg
      601 ctgcgaggat tatccgtctt ctccccattc cgctgcctcg gctgccaggc ctctggctgc
      661 tgaggagaag caggcccagt ctctgcaacc atccagcagc cgccgcagca gccattaccc
      721 ggctgcggtc cagggccaag cggcagcaga gcgaggggca tcagcgaccg ccaagtccag
      781 agccatttcc atcctgcaga agaagcctcg ccaccagcag cttctgccat ctctctcctc
      841 ctttttcttc agccacaggc tcccagacat gacagccatc atcaaagaga tcgttagcag
      901 aaacaaaagg agatatcaag aggatggatt cgacttagac ttgacctata tttatccaaa
      961 tattattgct atgggatttc ctgcagaaag acttgaaggt gtatacagga acaatattga
     1021 tgatgtagta aggtttttgg attcaaagca taaaaaccat tacaagatat acaatctatg
     1081 tgctgagaga cattatgaca ccgccaaatt taactgcaga gttgcacagt atccttttga
     1141 agaccataac ccaccacagc tagaacttat caaacccttc tgtgaagatc ttgaccaatg
     1201 gctaagtgaa gatgacaatc atgttgcagc aattcactgt aaagctggaa agggacggac
     1261 tggtgtaatg atttgtgcat atttattgca tcggggcaaa tttttaaagg cacaagaggc
     1321 cctagatttt tatggggaag taaggaccag agacaaaaag ggagtcacaa ttcccagtca
     1381 gaggcgctat gtatattatt atagctacct gctaaaaaat cacctggatt acagacccgt
     1441 ggcactgctg tttcacaaga tgatgtttga aactattcca atgttcagtg gcggaacttg
     1501 caatcctcag tttgtggtct gccagctaaa ggtgaagata tattcctcca attcaggacc
     1561 cacgcggcgg gaggacaagt tcatgtactt tgagttccct cagccattgc ctgtgtgtgg
     1621 tgatatcaaa gtagagttct tccacaaaca gaacaagatg ctcaaaaagg acaaaatgtt
     1681 tcacttttgg gtaaatacgt tcttcatacc aggaccagag gaaacctcag aaaaagtgga
     1741 aaatggaagt ctttgtgatc aggaaatcga tagcatttgc agtatagagc gtgcagataa
     1801 tgacaaggag tatcttgtac tcaccctaac aaaaaacgat cttgacaaag caaacaaaga
     1861 caaggccaac cgatacttct ctccaaattt taaggtgaaa ctatacttta caaaaacagt
     1921 agaggagcca tcaaatccag aggctagcag ttcaacttct gtgactccag atgttagtga
     1981 caatgaacct gatcattata gatattctga caccactgac tctgatccag agaatgaacc
     2041 ttttgatgaa gatcagcatt cacaaattac aaaagtctga tttttttttt cttatcaaga
     2101 gggataaaat accatgaaaa aaaaaaaact tgaataaact gaaatggacc tttttttttt
     2161 tttttttttt ttaaatggca ataggacatt gtgtcagatt gcagttatag gaacaattct
     2221 cttctcctga ccaatcttgt tttaccctat acatccacag ggttttgaca cttgttgtcc
     2281 agttaaaaaa aggttgtgta gctgtgtcat gtatatacct ttttgtgtca aaaggacatt
     2341 taaaattcaa ttaggataaa taaaagatgg cactttccca ttttattcca gttttataaa
     2401 aagtggagac aggctgatgt gtatacgcag gagtttttcc tttattttct gtcaccagct
     2461 gaagtggctg aagagctctg attcccgggt tcacgtccta cccctttgca cttgtggcaa
     2521 cagataagtt tgcagttggc taaggaagtt tctgcagggt tttgttagat tctaatgcat
     2581 gcacttgggt tgggaatgga gggaatgctc agaaaggaat gtttctacct gggctctgga
     2641 ccatacacca tctccagctc cttagatgca cctttcttta gcatgctcca cttactaatc
     2701 tggacatccg agagattggc tgctgtcctg ctgtttgttt gtgcatttta aagagcatat
     2761 tggtgctaga caaggcagct agagtgagta tatttgtagt ggggtacagg aatgaaccat
     2821 ctacagcatc ttaagaatcc acaaaggaag ggatataaaa aaagtggtca tagatagata
     2881 aaagacacag cagcaatgac ttaaccatac aaatgtggag gctttcaaca aaggatgggc
     2941 tggaaacaga aaatttgaca atgatttatt cagtatgctt tctcagttgt aatgactgct
     3001 ccatctccta tgtaatcaag gccagtgcta agagtcagat gctattagtc cctacatcag
     3061 tcaacacctt acctttattt ttattaattt tcaatcatat acctactgtg gatgcttcat
     3121 gtgctggctg ccagtttgtt tttctcctta aatattttat aattcttcac aggaaatttc
     3181 aacttgagat tcaacagtaa gcaggttttg tttttttttt ttcctagaga ttgatgatgc
     3241 gcgtcctcag tccagtggct gtcagacgtt cagccccttt gaccttacac attctattac
     3301 aatgagtttt gcagttttgc acattttttt taaatgtcat taactgttag ggaattttac
     3361 ttgaatactg aatacatata atgtgtatat taaaaaagtc attgtttgtg ttaaaaaaga
     3421 aattagagtt gcagtaaatt tacagcactg cacgaataat aaggcattga agtttttcag
     3481 tagaaattgt cctacagatg ctttatcgac ttgctattgg aagaatagat cttcttaaat
     3541 gtgcagtgtt gagtcacttc gttatagtgg tagagttggg attagggctt caattttact
     3601 tcttaaatat cattctatgt ttgatatgcc cagactgcat acaatttaaa gcaagagtac
     3661 aactactatc gtaatggtaa tgtgaagatg ctattacaaa ggatctcctc ccaacccctc
     3721 gggaatttgg tgtctttcaa attatatctt gaccttgaca tttgaatatc cagccattat
     3781 tagatttctt aatggtgtga agtcccattt tcaataactt attggtgctg aaattgttca
     3841 ctagctgtgg tctgacctag ttaatttaca agtacagatt gcataggacc cactagagaa
     3901 gcatttatag tttgatggta agtagattag gcagaacgcc atctaaaata ttcttagaaa
     3961 ataatgttga tgtattttcc atacctcatc agtttcactc aaccaataaa gtttttaaaa
     4021 ttgtaacaaa gctcttagga tttacacatt tatatttaaa cattgataca tgaatattga
     4081 ctgactgttg ataaagtcag agacaacttt tcctgagatc tcaccatgga aatctgtaca
     4141 cccccttgtc tttcctaaaa gctgaaagtg gctgactaaa atgcaaagca gctgttgatg
     4201 ttttgaagat agtgataaac actgttcttt gttagttttg ggcacagcat gctaaactat
     4261 aacttgtatt gttccaatat gtaacacaga gggccaggtc atgaataatg acattacaat
     4321 gggctgttgc actgttaata tttttccttt ggaatgtgaa ggtctgaatg agggttttga
     4381 ttttgaatgt ttcagtgttt ttgagaagcc ttgcttacat tttatggtgt agtcattgga
     4441 aatggaaaaa tggcattata tatatattat atatatataa atatatatat tatacatact
     4501 ctccttactt tatttcagtt accatcccca tagaatttga caagaattgc tatgactgaa
     4561 agggttttga gtcctaattc aaactttctt tatgacagta ttcacgatta gcctgaagtg
     4621 cattctgtag gtgatctctc ccgtgtttct ggaatgcttt cttagactct tggatgtgca
     4681 gcagcttatg tgtctgaaat gacttgaagg catcaccttt aagaaggctt acagttgggc
     4741 cccgtacatc ccaagtcctc tgtaattcct cttggacatt tttgccataa ttgtaaaagg
     4801 gtagttgaat taaatagcgt caccattctt tgctgtggca caggttataa acttaagtgg
     4861 agtttaccgg cagcatcaaa tgtttcagct ttaaaaataa aagtaggtta caagttacat
     4921 gtttagtttt agaaaatttg tgcaatatgt tcataacgat ggctgtggtt gccacaaagt
     4981 gcctcgttta cctttaaata ctgttaatgt gtcgtgcatg cagacggaag gggtggatct
     5041 gtgcactaaa cggggggctt ttactctagt attcggcaga gttgccttct acctgccagc
     5101 tcaaaagttc gatctgtttt catatagaat atatatacta aaaccatcca gtctgtaaaa
     5161 cagccttacc ccgattcagc ctcttcagat actcttgtgc tgtgcagcag tggctctgtg
     5221 tgtaaatgct atgcactgag gatacacaaa tatgacgtgt acaggataat gcctcatacc
     5281 aatcagatgt ccatttgtta ctgtgtttgt taacaaccct ttatctctta gtgttataaa
     5341 ctccacttaa aactgattaa agtctcattc ttgtcattgt gtgggtgttt tattaaatga
     5401 gagtatttat aattcaaatt gcttaaatcc attaaaatgt tcagtaatgg gcagccacat
     5461 atgattacaa agttcctgtg catttttcta tttttccccc tccttgctat ccttccaagc
     5521 aaagcatctt tctgtcatct tggtagacac atacctgtct actcatggtt aagaagagca
     5581 ctttaagcct tagtcatcac ttaataagtt attccaggca cagtaaaaag ttcaaggttc
     5641 ttggaaaacg gtgcttattt ctcttcttat aagccagatg tctgaagata gccctaaccc
     5701 caagaacggg cttgatgtct caggtctgtt ctgtggcttt ctgttttttt taacactgca
     5761 gttggccatc agcacatggg aggtttcatc gggacttgtc cagagtagta ggctcaaata
     5821 tactatctcc tttctaatat tcttaaaggc taaggagtcc tttcaatata acagtaagat
     5881 aacttgtgat gttttagaag taagcagacc attaatgtca atgtggagtc ttaatgttac
     5941 atgaagttga tagtttctct gtgacccatt taaaaataca aaccgagtag catgcaatta
     6001 tgtaaagaaa tatgaagatt atatgtagtc acacattttc tttagaattc ttagtttggt
     6061 gaaaacttga atataaaggt attttgattt atatgacatt ttgatgatat ttgaaaaaaa
     6121 ggaatttcct gacattttgc ttttagatca tgtcccccat tgtgctgtaa tttaagccaa
     6181 cttggttcag tgaatgccat caccatttcc attgagaatt taaaactcac cagtgtttaa
     6241 catgcaggct tctgagggct cccggagaat cagaccttaa gcccagttga tttacttcta
     6301 acgtgaaact tcgagttcct gtatactttg ctagataatt tgtggtacat ctaaagctta
     6361 gtcttaagtg gcttgtgtgt ggattttatt caacattctt gttgctaggg tagagagaaa
     6421 tgttgctgag tagaaacaag agtacccagt tcaatgtggt acagagagca gtccctaaaa
     6481 tctgtacaca gtgtaatgga ccactttagg agtcaagagg ctgatttttc ctatgaaatt
     6541 acattgcaac aggaagcctt ctagtatagt tccttttact gttagaatat gtttttatgc
     6601 atacgctata gctgctttcc catcttccaa caacaggtat caggatgtaa gcaagcttta
     6661 aacagtgtga agatggcagg atagtgtcat cggtaacagt cctctgactc taaatgtagt
     6721 tgctctgtaa cactttgtga atataacatc acaattctca tgtccttggg gggggggggc
     6781 atacccagta ttagtatgtt ttagtgacta agcaatcatt tttctgttta ctcatgtaca
     6841 ttttctcttt aaaactaaaa cctgtactgt gtatgtctcc aaagcctttt agcttagttt
     6901 ttaggaaatg aacactgaat ggatcacttt ttagtgtagc aggtatggga tatgtgcatt
     6961 atagagagac cttgtcagct ctctgggcct atttgaatgt ttattgttgg tgtgaggatg
     7021 gtaggggaat cagtaaatac aagttacgtt ggtttagcag agcaagctca gtgtgggtat
     7081 ttctctttga agcgtggtgc gtgacgcact gtgagtagag aatttggtca ccctttgagt
     7141 cctcttgcat tttgcaaact tgctcagcaa atgcgtacct accttgcccc ctaggtaaaa
     7201 gcaggaacta ctactgattt atctgtcact cagctgtctt tatatgtgtg cttctgtgac
     7261 ttgtatcaca caagaatctt aaagatttca caaattgtta ccttttagct ctgaatgttg
     7321 agtattctgg tgggctaaca acaagacaaa ctcttgacag tcatttgaga attttcatga
     7381 aacatttagc tgaaaacatt ttataattta tgaaaaaaat gtgttacctt aaacttttac
     7441 atatgtggga gacattaact gccatatttg agcatactga attttaaatt taaaataaag
     7501 ctgcatattt ttaaatgaaa tgtttaacaa ggattcatat tttttgtttt ttaagattaa
     7561 aaataattta tgtcttctca tgtggaacct catctgtcac aatggttaga ttatacagaa
     7621 tggagcaagg cttgtagtgg tttagcttac agtaaaattc ttaatgttta gatgtgttta
     7681 cttactggct gttatgtata cttttgagat tttccacctg ttctgtgtag ttttctaaat
     7741 gatactccta cttaaaaaca gcattttagt atctattttc tgtctccatt aaatggtcct
     7801 cattttctat tgagtttgga agtgtgcaca ttgtgtgtgt gtgtgtgtgt gtgtgtgtgc
     7861 acacgtgtgc gcgcccgtgc gtgtgtctat ttgtggagtt tgtatgggag aattagtttt
     7921 gaaagtgcta gaatagagat gaaatttggt tcaagtaaaa ttttcccact gggattttac
     7981 agtttattgt aataaaatgt taattttgga tgaccttgaa tattaatgaa tttgttagcc
     8041 tcttgatgtg tgcattaatg agatatatca aagttgtata ttaaaccaaa gttggagttg
     8101 tggaagtgtt tttatgaagt tccgtttggc taccaatgga cataagacta gaaatacctt
     8161 cctgtggaga atatttttcc tttaaacaat taaaaaggtt cattattttt gaaaaaaaaa
     8221 aaaaaaaaa
//
