LOCUS       NM_001093750            6489 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus patched domain containing 1 (Ptchd1), mRNA.
ACCESSION   NM_001093750 XM_142262 XM_911319
VERSION     NM_001093750.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Clayton,K.K., Stecyk,K.S., Guo,A.A., Chambers,A.R., Chen,K.,
            Hancock,K.E. and Polley,D.B.
  TITLE     Sound elicits stereotyped facial movements that provide a sensitive
            index of hearing abilities in mice
  JOURNAL   Curr Biol 34 (8), 1605-1620 (2024)
   PUBMED   38492568
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6489)
  AUTHORS   Pastore,S.F., Muhammad,T., Stan,C., Frankland,P.W., Hamel,P.A. and
            Vincent,J.B.
  TITLE     Neuronal transcription of autism gene PTCHD1 is regulated by a
            conserved downstream enhancer sequence
  JOURNAL   Sci Rep 13 (1), 20391 (2023)
   PUBMED   37990104
  REMARK    GeneRIF: Neuronal transcription of autism gene PTCHD1 is regulated
            by a conserved downstream enhancer sequence.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6489)
  AUTHORS   Kiral,F.R., Cakir,B., Tanaka,Y., Kim,J., Yang,W.S., Wehbe,F.,
            Kang,Y.J., Zhong,M., Sancer,G., Lee,S.H., Xiang,Y. and Park,I.H.
  TITLE     Generation of ventralized human thalamic organoids with thalamic
            reticular nucleus
  JOURNAL   Cell Stem Cell 30 (5), 677-688 (2023)
   PUBMED   37019105
REFERENCE   5  (bases 1 to 6489)
  AUTHORS   Maza,N., Wang,D., Kowalski,C., Stoveken,H.M., Dao,M., Sial,O.K.,
            Giles,A.C., Grill,B. and Martemyanov,K.A.
  TITLE     Ptchd1 mediates opioid tolerance via cholesterol-dependent effects
            on mu-opioid receptor trafficking
  JOURNAL   Nat Neurosci 25 (9), 1179-1190 (2022)
   PUBMED   35982154
REFERENCE   6  (bases 1 to 6489)
  AUTHORS   Ung,D.C., Iacono,G., Meziane,H., Blanchard,E., Papon,M.A.,
            Selten,M., van Rhijn,J.R., Montjean,R., Rucci,J., Martin,S.,
            Fleet,A., Birling,M.C., Marouillat,S., Roepman,R., Selloum,M.,
            Lux,A., Thepault,R.A., Hamel,P., Mittal,K., Vincent,J.B.,
            Dorseuil,O., Stunnenberg,H.G., Billuart,P., Nadif Kasri,N.,
            Herault,Y. and Laumonnier,F.
  TITLE     Ptchd1 deficiency induces excitatory synaptic and cognitive
            dysfunctions in mouse
  JOURNAL   Mol Psychiatry 23 (5), 1356-1367 (2018)
   PUBMED   28416808
  REMARK    GeneRIF: Results support that PTCHD1 deficiency induces a
            neurodevelopmental disorder causing excitatory synaptic
            dysfunction.
REFERENCE   7  (bases 1 to 6489)
  AUTHORS   Tora,D., Gomez,A.M., Michaud,J.F., Yam,P.T., Charron,F. and
            Scheiffele,P.
  TITLE     Cellular Functions of the Autism Risk Factor PTCHD1 in Mice
  JOURNAL   J Neurosci 37 (49), 11993-12005 (2017)
   PUBMED   29118110
  REMARK    GeneRIF: The Ptchd1 protein plays a critical role in the dentate
            gyrus and is associated with dendritic trafficking.
REFERENCE   8  (bases 1 to 6489)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   9  (bases 1 to 6489)
  AUTHORS   Wells,M.F., Wimmer,R.D., Schmitt,L.I., Feng,G. and Halassa,M.M.
  TITLE     Thalamic reticular impairment underlies attention deficit in
            Ptchd1(Y/-) mice
  JOURNAL   Nature 532 (7597), 58-63 (2016)
   PUBMED   27007844
  REMARK    GeneRIF: thalamic reticular nucleus-restricted deletion of Ptchd1
            leads to attention deficits and hyperactivity, both of which are
            rescued by pharmacological augmentation of small conductance
            calcium-dependent potassium channel activity
REFERENCE   10 (bases 1 to 6489)
  AUTHORS   Noor,A., Whibley,A., Marshall,C.R., Gianakopoulos,P.J., Piton,A.,
            Carson,A.R., Orlic-Milacic,M., Lionel,A.C., Sato,D., Pinto,D.,
            Drmic,I., Noakes,C., Senman,L., Zhang,X., Mo,R., Gauthier,J.,
            Crosbie,J., Pagnamenta,A.T., Munson,J., Estes,A.M., Fiebig,A.,
            Franke,A., Schreiber,S., Stewart,A.F., Roberts,R., McPherson,R.,
            Guter,S.J., Cook,E.H. Jr., Dawson,G., Schellenberg,G.D.,
            Battaglia,A., Maestrini,E., Jeng,L., Hutchison,T.,
            Rajcan-Separovic,E., Chudley,A.E., Lewis,S.M., Liu,X., Holden,J.J.,
            Fernandez,B., Zwaigenbaum,L., Bryson,S.E., Roberts,W., Szatmari,P.,
            Gallagher,L., Stratton,M.R., Gecz,J., Brady,A.F., Schwartz,C.E.,
            Schachar,R.J., Monaco,A.P., Rouleau,G.A., Hui,C.C., Lucy
            Raymond,F., Scherer,S.W. and Vincent,J.B.
  CONSRTM   Autism Genome Project Consortium
  TITLE     Disruption at the PTCHD1 Locus on Xp22.11 in Autism spectrum
            disorder and intellectual disability
  JOURNAL   Sci Transl Med 2 (49), 49ra68 (2010)
   PUBMED   20844286
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ061303.1, BC116312.1, AK036111.1, BX088539.6 and AK141827.1.
            
            On or before Jul 25, 2007 this sequence version replaced
            XM_911319.2, XM_142262.5.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC116312.1, AK036111.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 CJ061303.1         2-3
            3-207               BC116312.1         1-205
            208-2572            AK036111.1         3-2367
            2573-2684           BC116312.1         2571-2682
            2685-6469           BX088539.6         114338-118122       c
            6470-6489           AK141827.1         2710-2729
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 72.38 cM"
     gene            1..6489
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
                     /note="patched domain containing 1"
                     /db_xref="GeneID:211612"
                     /db_xref="MGI:MGI:2685233"
     exon            1..368
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..2684
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
                     /codon_start=1
                     /product="patched domain-containing protein 1"
                     /protein_id="NP_001087219.1"
                     /db_xref="CCDS:CCDS41188.1"
                     /db_xref="GeneID:211612"
                     /db_xref="MGI:MGI:2685233"
                     /translation="MLRQVLHRGLRTCFSRLGHFIASHPVFFASAPVLISILLGASFS
                     RYQVEESVEHLLAPQHSLAKIERNLVNSLFPVNRSKHRLYSDLQTPGRYGRVIVTSYQ
                     KANMLDQHHTDLILKLHTAVTKIQVPRPGFNYTFAHICVLNNDKTCIVDDIVHVLEEL
                     KNARATNRTNFAITYPITHLKDGRAVYNGHQLGGVTVHSKDRVKSAEAIQLTYYLQSI
                     NSLNDMVAERWESSFCDTVKLFQKSNSKVKIYPYTSSSLREDFQKTSRVSERYLVTSL
                     ILVVTMAILCCSMQDCVRSKPWLGLLGLVTISLATLTAAGIINLTGGKYNSTFLGVPF
                     VMLGHGLYGTFEMLSSWRKTREDQHVKERTAEVYADSMLSFSLTTAMYLVTFGIGASP
                     FTNIEAARIFCCNSCIAILFNYLYVLSFYGSSLVFTGYIENNYQHSIFCRKVPKPDVL
                     QEKPAWYRFLLTARFSEETAEGEEANTYESHLLVCFLKRYYCDWITNTYVKPFVVLFY
                     LIYISFALMGYLQVSEGSDLSNIVATATQTIEYTTAHQKYFNNYSPVIGFYIYESIEY
                     WNSSVQEDVLEYTKGFVRISWFESYLNYLRKLNVSTDLPKKNFTDMLRNSFLKTPQFS
                     HFQEDIIFSKKYNDEVDVVASRMFLVAKTMETNREELYDLLETLRRLSVTSKVKFIVF
                     NPSFVYMDRYASSLGAPLHNSCISALFLLFFSAFLVADSLINVWITLTVVSVEFGVIG
                     FMTLWKVELDCISVLCLIYGINYTIDNCAPLLSTFVLGKDFTRTKWVKNALEVHGVAI
                     LQSYLCYIVGLFPLAAVPSNLTCTLFRCLFLIAFVTFFHCFAILPVILTFLPPSKKKR
                     KEKKNPENREEIECVEMVDIDSTRVVDQITTV"
     exon            369..1029
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..6489
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4549..4554
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
                     /note="hexamer: AATAAA"
     polyA_site      4569
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
     regulatory      6382..6387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
                     /note="hexamer: ATTAAA"
     polyA_site      6405
                     /gene="Ptchd1"
                     /gene_synonym="9630036J22Rik; Gm387"
                     /note="major polyA site"
ORIGIN      
        1 gcccgctctg ctctaggatg ctgcggcagg ttctgcacag gggcttgagg acgtgtttct
       61 cccggcttgg ccacttcatt gccagtcacc cggtcttctt tgcttcggcg ccggtgctca
      121 tctccatcct gctcggcgcc agcttcagcc gctaccaggt cgaagagagc gtggagcacc
      181 tgctggcgcc ccagcacagc ctagccaaga tcgagcgcaa cctagtcaac agcctcttcc
      241 cggtcaaccg ctccaagcac cggctctact cggacctgca gacccctggg cgctacggcc
      301 gggtcattgt cacctcctac cagaaagcca acatgctaga ccaacatcac acggacctga
      361 tcttaaagtt gcatactgca gtgaccaaga tccaggttcc aaggcctggc tttaactata
      421 catttgctca catatgtgtc ctgaataatg ataagacttg cattgtggat gacatagtac
      481 acgtcctgga agagctaaag aatgctcggg ccaccaatcg gaccaatttt gctatcacat
      541 atccgatcac tcacttaaaa gatggaaggg ctgtgtataa tgggcaccaa ctcggaggtg
      601 tcactgtgca cagcaaggat cgggtgaaat ctgcagaggc cattcagctt acctactatc
      661 tgcaatcaat caacagtctc aatgacatgg tagctgagag gtgggagtcc agcttctgtg
      721 acactgtcaa actattccag aaatccaaca gcaaagtcaa aatatacccg tacacatcct
      781 catcactgag ggaagatttc cagaagacca gccgtgtatc agaacgttac ctggtcacca
      841 gcctgatcct ggtagtcacc atggctattc tgtgctgctc tatgcaggac tgtgtccgca
      901 gcaaaccctg gttaggccta cttgggttgg tgaccataag cctagccact ctcactgcag
      961 ccggaatcat caatctcact ggtggaaaat acaactccac cttcctggga gtccctttcg
     1021 tcatgctagg tcatggatta tacgggactt ttgaaatgtt atcctcgtgg aggaaaacta
     1081 gagaggacca acatgttaaa gagcgaactg cagaagtcta tgcagactcc atgctctcct
     1141 tctctctcac cactgccatg tacttggtca ccttcggcat cggggccagc cctttcacaa
     1201 acattgaggc agccaggatt ttctgctgca actcctgtat tgcaatcctc ttcaactacc
     1261 tctatgtact ctcattttat ggttccagcc ttgtgttcac tggctacata gaaaacaatt
     1321 accagcatag tatattctgt agaaaagttc cgaagcctga tgtattacag gagaagccag
     1381 catggtatag gtttcttctg acggctagat tcagtgagga aacagctgaa ggtgaggaag
     1441 caaacactta tgagagtcac ttattggtat gtttcctcaa acgctattac tgtgactgga
     1501 taaccaacac ctatgtcaag ccttttgtag tcctctttta ccttatttat atttcctttg
     1561 ccttaatggg ctatctgcag gtcagtgaag ggtcagacct tagtaacatc gtagcaactg
     1621 caacacaaac cattgagtac actactgccc accaaaagta ctttaacaac tacagtcctg
     1681 ttattgggtt ttacatatat gagtccatag aatattggaa ctctagtgtc caagaagatg
     1741 ttctagagta cactaagggt tttgtgcgga tatcctggtt tgaaagctat ttaaattatc
     1801 ttcggaaact caatgtgtcc actgacttgc ctaagaaaaa tttcacagac atgttgagga
     1861 attccttcct gaaaactcct caattttcac attttcaaga ggacatcatc ttctctaaaa
     1921 agtacaatga tgaggttgat gtagtggcct ctagaatgtt tttggtggcc aagactatgg
     1981 aaacaaacag agaagaactt tatgatctct tggagactct gaggaggctt tcagtcacct
     2041 ccaaggtgaa gttcattgtc tttaatccgt cttttgtgta catggatcga tatgcctcct
     2101 ctctgggagc ccccctgcac aattcctgca tcagtgcttt gttcctgctc ttcttctcgg
     2161 cattcctggt agcagattcg ctgatcaacg tctggatcac tctcacggtt gtgtctgtgg
     2221 agtttggagt gataggcttc atgacattat ggaaagtcga actggattgc atttctgtgc
     2281 tatgcttaat ttatggaatt aactacacaa ttgacaactg tgctccactg ttatccacat
     2341 ttgttctagg caaggatttt acaagaacta aatgggttaa aaatgccctg gaagtgcatg
     2401 gggtagctat tttacagagt tacctctgtt atattgttgg tctatttcct cttgcagctg
     2461 tgccttcaaa tctgacctgt acactgttca ggtgcttgtt tttaatagca tttgtcacct
     2521 tctttcactg ctttgccatt ttacctgtga tacttacttt cctgccaccc tctaagaaaa
     2581 aaaggaaaga gaagaaaaat cctgaaaacc gggaggaaat tgagtgtgta gaaatggtag
     2641 atattgatag tactcgagtg gttgaccaaa taaccacagt gtgatagtgt ctgcttcata
     2701 tattttcacc ctaggtctta ccaagaacaa aaagcttatg ttgctgaaac aattaaagtc
     2761 aaagttgttc tgttaaaaaa aaaaaaaaga taagaaacag acatgggaaa aaggcaaaag
     2821 gtgaggataa tgggtgtagc atctttaaat tttttaaagc aaaagggttt ctatgataat
     2881 ttatgataat ttacttacac acacacacaa acaaacatga cacgtagtat tttaagaaag
     2941 atcaaacaaa ggaatgctta ttaataagca tgatcctatt ttacctacac tgcacaagtt
     3001 actggtattg taataagata caactgattg caaagtcagc tgttatgaca aatataggtt
     3061 taagcattgt tcaaacaagg ttttctaaac ttctatcaag aagtaactct acttaggttc
     3121 tgtcatttga ggtcttcatt gtttcatcta gcacctcaac actcaaggag attctgtgaa
     3181 agttctgatc agcaagagga agccagaact tttaaaccag atgtgataga ctgaatatct
     3241 ccacaaattg aaattgagta tcacccccaa attctcttag gtgagccaag tttatttttt
     3301 tcttgaatga acttgtcata ttcctccact gatgcacctt ttaacacctg tatggtttag
     3361 ttaatgcacc cagaaatcca tgacagctcc agcattacag aactggggca attcaatttc
     3421 agaatatctt gttacctgtc atgaaaaggg tctactttat tagttacagc ccttggcagg
     3481 aacctctgag ggtgcaaagc cacagagtca agctgaaata tctggcatgt tggaagaaaa
     3541 gcaggtatct atttgaacat acagagtatt tctatattac caaaaaagaa aagccagctg
     3601 gtacagatgt ttgtggtttg gccctacatg cacttttgca tggatgccca gaaacaacat
     3661 ctgtcccaaa ataacatctg cccacatata tgaggaaaag aaaataaaca ccaaaatggt
     3721 tattggccaa caagtgccag attttttttt tttttttttt tttttttttt tttttttttt
     3781 ttttttgcta tgtgataagt tctacttgac ttagtggaga aaaggaaaac ctttgataag
     3841 actaagactt ggcaatgaga aactttataa aagtgcttgg agataaaggg ctcaaaggaa
     3901 aataggtggg atttcctgga taggaataat taccagtaca aatcaatcca taacagcagc
     3961 caaacttccc tgctaaactg gctgtaccgc ccctccccta aaatgccttt ttgctatctc
     4021 tctacttcat tataaggctc tctgtacatt aacatgccca gatatttata cagttgaaca
     4081 gttgtatcta caagggcaat gattcatcaa tctaaacaat gacagtttga tctgaagggg
     4141 gggtatgtat gctgccctca gacccctgta tttctttaaa tccataaaaa ttaagccaaa
     4201 tcctgggtta catttgaggc aaacagctac aatgtctttc ctttttaaag atgtagcttc
     4261 tttagtcacc tatagtggta agcatttcca aaaggcatat tacctttcag gactttagca
     4321 gacatattat actacttctt cattctccat aacgggtgaa acagaccttg aagaatgaaa
     4381 ctggcccagg ttacactact ataggaggtc catcatttca acatgctgaa gacacagcag
     4441 gacaattgta tattttcctg taggtagcta ctacccctta aaattcatac ataaggtggg
     4501 aacataaggc ttcattggct gtggaacaaa catgtcttta atgtctggaa taaaaatact
     4561 ttggattaag gttggctatt atccttctaa atagtaaaaa tgcccaaatg attgtgatgt
     4621 aatcacagca agtgataatc tatgcttttg ctctcatgtg cttttgtcca aaaagttcta
     4681 acaaatattc taatttctca gtctcccctg cagacacatt aagcagtcat agtaatgtgt
     4741 ttgttcactg aacaagaaga ggaggagttt taaatctaaa gacaagtata ttcttcacaa
     4801 aatggcaagt ttgcatacta gatgctcaat cagttctctc tcttatggtc ctgcaaccag
     4861 gaaattattt atcaaaaaca actgaagaag ggattggttt tattcttaaa ttatatgctg
     4921 ctaaactgtt ttctgaacag tgtgttctaa acctgccatt gatttagtgt cagccatgtg
     4981 aaacatctca gcttagtgaa ttcacaaaac tgacccaaca catgcaccaa ttaaatggat
     5041 tttgttgaga atttaatcat caatttggtc aaccagagtg acttactgtg gaactttgtt
     5101 ttatgacacg tagtagtttt ccaacttgat tgagtctctg tgtatcttga gatgttgtat
     5161 ttttaaatgg agggtatttt caaacttgtc acattctcca aacttgtcag cttctctttt
     5221 cagcacttaa aattaagtta tgaaattctg actgtaaaat gaatttttct attggaggac
     5281 tgcatgccaa aagttattta ttgttgagca attattatgt atttgttcat atttattatt
     5341 gattattaat agtgatctga cagcatcagt ctaacttcac catggagatg acactgtaga
     5401 aatagctcaa aatattttaa gtcttcataa aatttccctt tttaaattgc taaaagggaa
     5461 ggaatgatct cagcaaatca gttttttctt ttaattaatt ccaaccaaga cattaatgtt
     5521 cactttacca aagaaacatt ggttggcttt aagttagtgt ttggtgtcca tttaccaaag
     5581 aaaatgttcc atcatcaaaa tatttttcct ggtcaactaa actactgtga tgcttgggcc
     5641 ttctgaacga agataagaaa agccaaggta agaggaataa tattctctcc tttggtagaa
     5701 tccagcagaa gatgtaaatt cacaagggaa ttggtgttta caaaaaagaa aacaataatg
     5761 gaaattggat gttgttattc tgacatgcag taatgttcca acaaggacca tgagggatac
     5821 acaggctaaa agtacatttt gttccagaga aaatgtaaaa caaaccttca tgaaaatctc
     5881 tgtccattgt tcctgctaat aataactctt tgtgtgtgtg tttgtgtgtt tgttttgggc
     5941 tagtttcatt tttgcttgtt tgattaatgg gttggctggg tttaattttt attggggaag
     6001 gggttgtctt cattttgctt ttatttagtt tgtgggtttt gttcatgtgg cttctgcttc
     6061 tgctctcact ctcacctcta tattttcagt cagaagtgaa ataattagag gtctcctcca
     6121 tggccgctgt tattcatgca atctactgct ctaaaaagac ccttgagaaa ctgtttattt
     6181 taaataacca ctccccaatt gtctgtggca ttctaagact atgacactac cttcctccaa
     6241 ccccagcaac agaccttggc tgtgtccctc atgagaacag gtcaaatgaa gaaccgccat
     6301 cggggtgtgg tatggtatga ggcaaagcct cttgaacaca aatggtaccc tcttcataat
     6361 ggctttattc ctttggatcc tattaaagat tccctgcagc ttgtaccagt gtttgacctc
     6421 tgtgtatctc tgtttcttca tctgtacagt ggggataaaa aaaaaaaaac agtaaatatc
     6481 tttaaattc
//
