LOCUS       NM_008957               7323 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus patched 1 (Ptch1), transcript variant 1, mRNA.
ACCESSION   NM_008957
VERSION     NM_008957.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Zhu,X., Xu,M., Portal,C., Lin,Y., Ferdinand,A., Peng,T.,
            Morrisey,E.E., Dlugosz,A.A., Castellano,J.M., Lee,V., Seykora,J.T.,
            Wong,S.Y., Iomini,C. and Millar,S.E.
  TITLE     Identification of Meibomian gland stem cell populations and
            mechanisms of aging
  JOURNAL   Nat Commun 16 (1), 1663 (2025)
   PUBMED   39955307
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Zhang,C., Zheng,Y., Qu,Y., Huang,R., Huang,H., Li,J., Qiu,M. and
            Li,F.
  TITLE     Transcriptional factor ISL1 regulates palate development by tuning
            the SHH cascade
  JOURNAL   FEBS J 292 (4), 851-863 (2025)
   PUBMED   39704783
REFERENCE   3  (bases 1 to 7323)
  AUTHORS   Palacio,V., Pancho,A., Morabito,A., Malkmus,J., He,Z., Soussi,G.,
            Zeller,R., Treutlein,B. and Zuniga,A.
  TITLE     Single-cell profiling of penta- and tetradactyl mouse limb buds
            identifies mesenchymal progenitors controlling digit numbers and
            identities
  JOURNAL   Nat Commun 16 (1), 1226 (2025)
   PUBMED   39890843
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7323)
  AUTHORS   Fan,J.J., Erickson,A.W., Carrillo-Garcia,J., Wang,X., Skowron,P.,
            Wang,X., Chen,X., Shan,G., Dou,W., Bahrampour,S., Xiong,Y.,
            Dong,W., Abeysundara,N., Francisco,M.A., Pusong,R.J., Wang,W.,
            Li,M., Ying,E., Suarez,R.A., Farooq,H., Holgado,B.L., Wu,X.,
            Daniels,C., Dupuy,A.J., Cadinanos,J., Bradley,A., Bagchi,A.,
            Moriarity,B.S., Largaespada,D.A., Morrissy,A.S., Ramaswamy,V.,
            Mack,S.C., Garzia,L., Dirks,P.B., Li,X., Wanggou,S., Egan,S.,
            Sun,Y., Taylor,M.D. and Huang,X.
  TITLE     A forward genetic screen identifies potassium channel essentiality
            in SHH medulloblastoma maintenance
  JOURNAL   Dev Cell (2025) In press
   PUBMED   39862856
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   5  (bases 1 to 7323)
  AUTHORS   Tanno,B., Fratini,E., Leonardi,S., Novelli,F., Pisano,V.,
            Mancuso,M. and Pazzaglia,S.
  TITLE     Dissecting the Impact of Genetic Background on Oncogenic Response
            to Radiation Exposure in the Ptch1+/- Mouse Model
  JOURNAL   Cells 13 (22), 1912 (2024)
   PUBMED   39594660
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7323)
  AUTHORS   Epstein,D.J., Marti,E., Scott,M.P. and McMahon,A.P.
  TITLE     Antagonizing cAMP-dependent protein kinase A in the dorsal CNS
            activates a conserved Sonic hedgehog signaling pathway
  JOURNAL   Development 122 (9), 2885-2894 (1996)
   PUBMED   8787761
REFERENCE   7  (bases 1 to 7323)
  AUTHORS   Hahn,H., Wicking,C., Zaphiropoulous,P.G., Gailani,M.R., Shanley,S.,
            Chidambaram,A., Vorechovsky,I., Holmberg,E., Unden,A.B.,
            Gillies,S., Negus,K., Smyth,I., Pressman,C., Leffell,D.J.,
            Gerrard,B., Goldstein,A.M., Dean,M., Toftgard,R.,
            Chenevix-Trench,G., Wainwright,B. and Bale,A.E.
  TITLE     Mutations of the human homolog of Drosophila patched in the nevoid
            basal cell carcinoma syndrome
  JOURNAL   Cell 85 (6), 841-851 (1996)
   PUBMED   8681379
REFERENCE   8  (bases 1 to 7323)
  AUTHORS   Hahn,H., Christiansen,J., Wicking,C., Zaphiropoulos,P.G.,
            Chidambaram,A., Gerrard,B., Vorechovsky,I., Bale,A.E., Toftgard,R.,
            Dean,M. and Wainwright,B.
  TITLE     A mammalian patched homolog is expressed in target tissues of sonic
            hedgehog and maps to a region associated with developmental
            abnormalities
  JOURNAL   J Biol Chem 271 (21), 12125-12128 (1996)
   PUBMED   8647801
REFERENCE   9  (bases 1 to 7323)
  AUTHORS   Sweet,H.O., Bronson,R.T., Donahue,L.R. and Davisson,M.T.
  TITLE     Mesenchymal dysplasia: a recessive mutation on chromosome 13 of the
            mouse
  JOURNAL   J Hered 87 (2), 87-95 (1996)
   PUBMED   8830098
REFERENCE   10 (bases 1 to 7323)
  AUTHORS   Goodrich,L.V., Johnson,R.L., Milenkovic,L., McMahon,J.A. and
            Scott,M.P.
  TITLE     Conservation of the hedgehog/patched signaling pathway from flies
            to mice: induction of a mouse patched gene by Hedgehog
  JOURNAL   Genes Dev 10 (3), 301-312 (1996)
   PUBMED   8595881
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BF464755.1, AB164615.1, CK639136.1, AK138524.1, CF165613.1,
            CD545333.1, CK636036.1, CN531162.1, AK163736.1, CO433508.1,
            CB520013.1, AK147626.1 and BU700582.1.
            
            On Jun 16, 2016 this sequence version replaced NM_008957.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: U46155.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-497               BF464755.1         1-497
            498-1148            AB164615.1         199-849
            1149-1726           CK639136.1         147-724
            1727-2091           AK138524.1         356-720
            2092-2442           CF165613.1         100-450
            2443-2892           CD545333.1         81-530
            2893-3480           CK636036.1         119-706
            3481-4053           CN531162.1         87-659
            4054-4487           AK163736.1         408-841
            4488-4488           CO433508.1         634-634
            4489-5128           AK163736.1         843-1482
            5129-5680           CB520013.1         181-732
            5681-7050           AK147626.1         6032-7401
            7051-7323           BU700582.1         1-273               c
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 32.8 cM"
     gene            1..7323
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /note="patched 1"
                     /db_xref="GeneID:19206"
                     /db_xref="MGI:MGI:105373"
     exon            1..374
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..4520
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /note="isoform a is encoded by transcript variant 1;
                     protein patched homolog 1; Ptch1 protein +12b; mesenchymal
                     dysplasia"
                     /codon_start=1
                     /product="protein patched homolog 1 isoform a"
                     /protein_id="NP_032983.1"
                     /db_xref="CCDS:CCDS26592.1"
                     /db_xref="GeneID:19206"
                     /db_xref="MGI:MGI:105373"
                     /translation="MASAGNAAGALGRQAGGGRRRRTGGPHRAAPDRDYLHRPSYCDA
                     AFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGL
                     KAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEA
                     LLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPL
                     DCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHG
                     YMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVK
                     NATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEV
                     VHQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSK
                     SQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAF
                     SETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVV
                     VVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTR
                     YSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDN
                     LSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVI
                     LLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADY
                     PNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMP
                     NNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVS
                     NDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSD
                     FVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCA
                     VFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVAL
                     AFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILT
                     VLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPGHTNNGS
                     DSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDSRH
                     QPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPS
                     NRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPR
                     GGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWGSSSN"
     exon            375..567
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            568..757
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            758..827
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            828..919
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1118
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1240
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1388
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1520
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1676
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1775
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1901
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2020
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2423
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2733
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2876
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            2877..3060
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3061..3341
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3479
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3480..3622
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3623..3722
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3723..3977
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3978..4521
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            4522..7306
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7279..7284
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /note="hexamer: AATAAA"
     polyA_site      7306
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /note="major polyA site"
ORIGIN      
        1 gcggccgcaa agacctcggg actcacgcgc aatgtggcaa tggaaggcgc agggtctgag
       61 tccccggcag cggccacggc cgcagcaccc gcagcgcccg ccgtgtgagc ggcagcagcg
      121 ggtctgtcac ccggagccgg agtccccggc ggccagcagc gtcctcgcga gccgagcgcc
      181 caggcgcgcc cggagcccgc ggcggcggcg gcaacatggc ctcggctggt aacgccgccg
      241 gggccctggg caggcaggcc ggcggcggga ggcgcagacg gaccggggga ccgcaccgcg
      301 ccgcgccgga ccgggactat ctgcaccggc ccagctactg cgacgccgcc ttcgctctgg
      361 agcagatttc caaggggaag gctactggcc ggaaagcgcc gctgtggctg agagcgaagt
      421 ttcagagact cttatttaaa ctgggttgtt acattcaaaa gaactgcggc aagtttttgg
      481 ttgtgggtct cctcatattt ggggccttcg ctgtgggatt aaaggcagct aatctcgaga
      541 ccaacgtgga ggagctgtgg gtggaagttg gtggacgagt gagtcgagaa ttaaattata
      601 cccgtcagaa gataggagaa gaggctatgt ttaatcctca actcatgata cagactccaa
      661 aagaagaagg cgctaatgtt ctgaccacag aggctctcct gcaacacctg gactcagcac
      721 tccaggccag tcgtgtgcac gtctacatgt ataacaggca atggaagttg gaacatttgt
      781 gctacaaatc aggggaactt atcacggaga caggttacat ggatcagata atagaatacc
      841 tttacccttg cttaatcatt acacctttgg actgcttctg ggaaggggca aagctacagt
      901 ccgggacagc atacctccta ggtaagcctc ctttacggtg gacaaacttt gaccccttgg
      961 aattcctaga agagttaaag aaaataaact accaagtgga cagctgggag gaaatgctga
     1021 ataaagccga agttggccat gggtacatgg accggccttg cctcaaccca gccgacccag
     1081 attgccctgc cacagcccct aacaaaaatt caaccaaacc tcttgatgtg gcccttgttt
     1141 tgaatggtgg atgtcaaggt ttatccagga agtatatgca ttggcaggag gagttgattg
     1201 tgggtggtac cgtcaagaat gccactggaa aacttgtcag cgctcacgcc ctgcaaacca
     1261 tgttccagtt aatgactccc aagcaaatgt atgaacactt caggggctac gactatgtct
     1321 ctcacatcaa ctggaatgaa gacagggcag ccgccatcct ggaggcctgg cagaggactt
     1381 acgtggaggt ggttcatcaa agtgtcgccc caaactccac tcaaaaggtg cttcccttca
     1441 caaccacgac cctggacgac atcctaaaat ccttctctga tgtcagtgtc atccgagtgg
     1501 ccagcggcta cctactgatg cttgcctatg cctgtttaac catgctgcgc tgggactgct
     1561 ccaagtccca gggtgccgtg gggctggctg gcgtcctgtt ggttgcgctg tcagtggctg
     1621 caggattggg cctctgctcc ttgattggca tttcttttaa tgctgcgaca actcaggttt
     1681 tgccgtttct tgctcttggt gttggtgtgg atgatgtctt cctcctggcc catgcattca
     1741 gtgaaacagg acagaataag aggattccat ttgaggacag gactggggag tgcctcaagc
     1801 gcaccggagc cagcgtggcc ctcacctcca tcagcaatgt caccgccttc ttcatggccg
     1861 cattgatccc tatccctgcc ctgcgagcgt tctccctcca ggctgctgtg gtggtggtat
     1921 tcaattttgc tatggttctg ctcatttttc ctgcaattct cagcatggat ttatacagac
     1981 gtgaggacag aagattggat attttctgct gtttcacaag cccctgtgtc agcagggtga
     2041 ttcaagttga gccacaggcc tacacagagc ctcacagtaa cacccggtac agccccccac
     2101 ccccatacac cagccacagc ttcgcccacg aaacccatat cactatgcag tccaccgttc
     2161 agctccgcac agagtatgac cctcacacgc acgtgtacta caccaccgcc gagccacgct
     2221 ctgagatctc tgtacagcct gttaccgtca cccaggacaa cctcagctgt cagagtcccg
     2281 agagcaccag ctctaccagg gacctgctct cccagttctc agactccagc ctccactgcc
     2341 tcgagccccc ctgcaccaag tggacactct cttcgtttgc agagaagcac tatgctcctt
     2401 tcctcctgaa acccaaagcc aaggttgtgg taatccttct tttcctgggc ttgctggggg
     2461 tcagccttta tgggaccacc cgagtgagag acgggctgga cctcacggac attgttcccc
     2521 gggaaaccag agaatatgac ttcatagctg cccagttcaa gtacttctct ttctacaaca
     2581 tgtatatagt cacccagaaa gcagactacc cgaatatcca gcacctactt tacgaccttc
     2641 ataagagttt cagcaatgtg aagtatgtca tgctggagga gaacaagcaa cttccccaaa
     2701 tgtggctgca ctactttaga gactggcttc aaggacttca ggatgcattt gacagtgact
     2761 gggaaactgg gaggatcatg ccaaacaatt ataaaaatgg atcagatgac ggggtcctcg
     2821 cttacaaact cctggtgcag actggcagcc gagacaagcc catcgacatt agtcagttga
     2881 ctaaacagcg tctggtagac gcagatggca tcattaatcc gagcgctttc tacatctacc
     2941 tgaccgcttg ggtcagcaac gaccctgtag cttacgctgc ctcccaggcc aacatccggc
     3001 ctcaccggcc ggagtgggtc catgacaaag ccgactacat gccagagacc aggctgagaa
     3061 tcccagcagc agagcccatc gagtacgctc agttcccttt ctacctcaac ggcctacgag
     3121 acacctcaga ctttgtggaa gccatagaaa aagtgagagt catctgtaac aactatacga
     3181 gcctgggact gtccagctac cccaatggct accccttcct gttctgggag caatacatca
     3241 gcctgcgcca ctggctgctg ctatccatca gcgtggtgct ggcctgcacg tttctagtgt
     3301 gcgcagtctt cctcctgaac ccctggacgg ccgggatcat tgtcatggtc ctggctctga
     3361 tgaccgttga gctctttggc atgatgggcc tcattgggat caagctgagt gctgtgcctg
     3421 tggtcatcct gattgcatct gttggcatcg gagtggagtt caccgtccac gtggctttgg
     3481 cctttctgac agccattggg gacaagaacc acagggctat gctcgctctg gagcacatgt
     3541 ttgctcccgt tctggacggt gctgtgtcca ctctgctggg tgtactgatg cttgcagggt
     3601 ccgaatttga tttcattgtc agatacttct ttgccgtcct ggccattctc accgtcttgg
     3661 gggttctcaa tggactggtt ctgctgcctg tcctcttatc cttctttgga ccgtgtcctg
     3721 aggtgtctcc agccaatggc ctaaaccgac tgcccactcc ttcgcctgag ccgcctccaa
     3781 gtgtcgtccg gtttgccgtg cctcctggtc acacgaacaa tgggtctgat tcctccgact
     3841 cggagtacag ctctcagacc acggtgtctg gcatcagtga ggagctcagg caatacgaag
     3901 cacagcaggg tgccggaggc cctgcccacc aagtgattgt ggaagccaca gaaaaccctg
     3961 tctttgcccg gtccactgtg gtccatccgg actccagaca tcagcctccc ttgacccctc
     4021 ggcaacagcc ccacctggac tctggctcct tgtcccctgg acggcaaggc cagcagcctc
     4081 gaagggatcc ccctagagaa ggcttgcggc caccccccta cagaccgcgc agagacgctt
     4141 ttgaaatttc tactgaaggg cattctggcc ctagcaatag ggaccgctca gggccccgtg
     4201 gggcccgttc tcacaaccct cggaacccaa cgtccaccgc catgggcagc tctgtgccca
     4261 gctactgcca gcccatcacc actgtgacgg cttctgcttc ggtgactgtt gctgtgcatc
     4321 ccccgcctgg acctgggcgc aacccccgag gggggccctg tccaggctat gagagctacc
     4381 ctgagactga tcacggggta tttgaggatc ctcatgtgcc ttttcatgtc aggtgtgaga
     4441 ggagggactc aaaggtggag gtcatagagc tacaggacgt ggaatgtgag gagaggccgt
     4501 gggggagcag ctccaactga gggtaattaa aatctgaagc aaagaggcca aagattggaa
     4561 agccccgccc ccacctcttt ccagaactgc ttgaagagaa ctgcttggaa ttatgggaag
     4621 gcagttcatt gttactgtaa ctgattgtat tattttgtga aatatttcta taaatattta
     4681 aaaggtgtac acatgtaata tacatggaaa tgctgtacag tctatttcct ggggcctctc
     4741 cactcctgcc ccagagtggg gagaccacag gggccctttc ccctgtgtac attggtctct
     4801 gtgccacaac caagcttaac ttagttttaa aaaaaatctc ccagcatatg tcgctgctgc
     4861 ttaaatattg tataatttac ttgtataatt ctatgcaaat attgcttatg taataggatt
     4921 atttgtaaag gtttctgttt aaaatatttt aaatttgcat atcacaaccc tgtggtagga
     4981 tgaattgtta ctgttaactt ttgaacacgc tatgcgtggt aattgtttaa cgagcagaca
     5041 tgaagaaaac aggttaatcc cagtggcttc tctaggggta gttgtatatg gttcgcatgg
     5101 gtggatgtgt gtgtgcatgt gactttccaa tgtactgtat tgtggtttgt tgttgttgtt
     5161 gctgttgttg ttcatttttg tgttttttgt tgctttgtat gatcttagct ctggcctagg
     5221 tgggctggga aggtccaggt ctttttctgt cgtgatgctg gtggaaaggt gaccccaatc
     5281 atctgtccta ttctctggga ctattcaaga gaagccagat ttgcttcatg cctgtgtgtg
     5341 gcagcttctg aggtcatggg tagccctcca agcacctctg cttgggtttc aaagagaaga
     5401 tgttctcaca aacattgtgc tgcctattag ggcctctata tagtcagcag tcagcactag
     5461 tggtgaaaga ttggacaatg ttgcctgatg gtgagactca gccgggtcat gagattaccc
     5521 tctaggggtg tgtggttgtt cctttctgat gatcacacat acacacagcc tcccaccccc
     5581 atactccaga tacaggccca aaaaaaccta tttcactgaa ggtctatatt cgagccttta
     5641 caaatgatag tccctccctt gctatgactg cctggttgga gcaggctagg ctaatcgtgg
     5701 ttcagtcaac tgtgtgggga gagtttctaa ggactctttt ccacacggtc ttttcctgtg
     5761 tatactttct tctcttcccc actgttagca tccatccagt gtgatgtctg tgaaagcagg
     5821 aagatgccac ctcgtaaaag catatagggt aagctctttg aaccaggtgt tgggctttag
     5881 agtttgattg tgttctcctt ccccaaggtt tgggtttctt tgtctctggt cagcaactag
     5941 tagataggga aggaaatgcc cttattcctt gcaagtgaac attgaggact tgtctcagag
     6001 gaaggaagcc tagtgctcat ggaagctgtt gtgaggaatg tactctgtct gcatgcagct
     6061 tccctgaatc cctcctatcc ttcatggaaa aatttaagga gatgggtccc tagtagtacc
     6121 aataatctag tgccctcaga tcctaaaatt tatcccccaa attgtaaggt actaattgga
     6181 agccacctgt gactaccgtg tgtgtgtgtg tctgtgtctg tctgtatatc cgtgtgcgcg
     6241 tgcgcgccag aagccctggc catccactgc tactgtctta atgcctgtca agccactgtc
     6301 tgcctaccca acaccagctc tgctcggata tcttgcaccc tgagttgagg agggagatgt
     6361 agagtgcgga agccaccttg gctgtggttc ttgattgtgt ccctcatgcc tgagccttgt
     6421 gcatgtggca gaaggaagtt tgtacagcct ctcggcttct gtgcatcatc atgagtccca
     6481 tcagccaagg caggaaggac acagagctgg caggagacta aagtcagaga gtgtgtgtct
     6541 ctgtctgtct ctctcatagt tttattttgt ctgtattgtt tgttcatttg gatgttttaa
     6601 tttgtaaaag aaaagatctt tgctgatatt tataattttg tatcataaga atgtcctcca
     6661 gaatttgtca tgccagttta taacaagaag aaaaaattgc agggatttta tttctattgg
     6721 aaacacttat tgcagttatg ttttactttt gaacagaagt tttatttgta tagagtgctt
     6781 actaatgtta aatagttcag agtatataac atttacatta aggactcatg gtaggtttta
     6841 gtgtaaggag tttaaaggaa ataaatattc aaactgggtc tcgtctgcca atttgggtgg
     6901 aaatgagttt gtgtcacttc aattacaaag atgaaagtat gccatataat ttatttatat
     6961 gaaaatttat ttttgtagtg tacatagtag tcatcaagtc tttcgacaga agtatatttt
     7021 taaagaattt atatgtgatg aaatccataa tgtctggaac tttgctgaga catgggtgtg
     7081 aggacacgtt tcgttataaa tgacagcaag ggagagaaga gagtatgttt taacagtgtt
     7141 aggagagtac acgtgagcag tgatccatgt gattggaaag tatcggtgtg aacatggtga
     7201 cctagtgcgg ttctcagatg aaaatgtaca aaactctcta aatattaatg ttcaaacact
     7261 gatagaaatt ctaacatgaa taaagataat ataacttgtt ggtttaaaaa aaaaaaaaaa
     7321 aaa
//
