LOCUS       NM_001328514            7318 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus patched 1 (Ptch1), transcript variant 2, mRNA.
ACCESSION   NM_001328514
VERSION     NM_001328514.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Zhu,X., Xu,M., Portal,C., Lin,Y., Ferdinand,A., Peng,T.,
            Morrisey,E.E., Dlugosz,A.A., Castellano,J.M., Lee,V., Seykora,J.T.,
            Wong,S.Y., Iomini,C. and Millar,S.E.
  TITLE     Identification of Meibomian gland stem cell populations and
            mechanisms of aging
  JOURNAL   Nat Commun 16 (1), 1663 (2025)
   PUBMED   39955307
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Zhang,C., Zheng,Y., Qu,Y., Huang,R., Huang,H., Li,J., Qiu,M. and
            Li,F.
  TITLE     Transcriptional factor ISL1 regulates palate development by tuning
            the SHH cascade
  JOURNAL   FEBS J 292 (4), 851-863 (2025)
   PUBMED   39704783
REFERENCE   3  (bases 1 to 7318)
  AUTHORS   Palacio,V., Pancho,A., Morabito,A., Malkmus,J., He,Z., Soussi,G.,
            Zeller,R., Treutlein,B. and Zuniga,A.
  TITLE     Single-cell profiling of penta- and tetradactyl mouse limb buds
            identifies mesenchymal progenitors controlling digit numbers and
            identities
  JOURNAL   Nat Commun 16 (1), 1226 (2025)
   PUBMED   39890843
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7318)
  AUTHORS   Fan,J.J., Erickson,A.W., Carrillo-Garcia,J., Wang,X., Skowron,P.,
            Wang,X., Chen,X., Shan,G., Dou,W., Bahrampour,S., Xiong,Y.,
            Dong,W., Abeysundara,N., Francisco,M.A., Pusong,R.J., Wang,W.,
            Li,M., Ying,E., Suarez,R.A., Farooq,H., Holgado,B.L., Wu,X.,
            Daniels,C., Dupuy,A.J., Cadinanos,J., Bradley,A., Bagchi,A.,
            Moriarity,B.S., Largaespada,D.A., Morrissy,A.S., Ramaswamy,V.,
            Mack,S.C., Garzia,L., Dirks,P.B., Li,X., Wanggou,S., Egan,S.,
            Sun,Y., Taylor,M.D. and Huang,X.
  TITLE     A forward genetic screen identifies potassium channel essentiality
            in SHH medulloblastoma maintenance
  JOURNAL   Dev Cell (2025) In press
   PUBMED   39862856
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   5  (bases 1 to 7318)
  AUTHORS   Tanno,B., Fratini,E., Leonardi,S., Novelli,F., Pisano,V.,
            Mancuso,M. and Pazzaglia,S.
  TITLE     Dissecting the Impact of Genetic Background on Oncogenic Response
            to Radiation Exposure in the Ptch1+/- Mouse Model
  JOURNAL   Cells 13 (22), 1912 (2024)
   PUBMED   39594660
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7318)
  AUTHORS   Epstein,D.J., Marti,E., Scott,M.P. and McMahon,A.P.
  TITLE     Antagonizing cAMP-dependent protein kinase A in the dorsal CNS
            activates a conserved Sonic hedgehog signaling pathway
  JOURNAL   Development 122 (9), 2885-2894 (1996)
   PUBMED   8787761
REFERENCE   7  (bases 1 to 7318)
  AUTHORS   Hahn,H., Wicking,C., Zaphiropoulous,P.G., Gailani,M.R., Shanley,S.,
            Chidambaram,A., Vorechovsky,I., Holmberg,E., Unden,A.B.,
            Gillies,S., Negus,K., Smyth,I., Pressman,C., Leffell,D.J.,
            Gerrard,B., Goldstein,A.M., Dean,M., Toftgard,R.,
            Chenevix-Trench,G., Wainwright,B. and Bale,A.E.
  TITLE     Mutations of the human homolog of Drosophila patched in the nevoid
            basal cell carcinoma syndrome
  JOURNAL   Cell 85 (6), 841-851 (1996)
   PUBMED   8681379
REFERENCE   8  (bases 1 to 7318)
  AUTHORS   Hahn,H., Christiansen,J., Wicking,C., Zaphiropoulos,P.G.,
            Chidambaram,A., Gerrard,B., Vorechovsky,I., Bale,A.E., Toftgard,R.,
            Dean,M. and Wainwright,B.
  TITLE     A mammalian patched homolog is expressed in target tissues of sonic
            hedgehog and maps to a region associated with developmental
            abnormalities
  JOURNAL   J Biol Chem 271 (21), 12125-12128 (1996)
   PUBMED   8647801
REFERENCE   9  (bases 1 to 7318)
  AUTHORS   Sweet,H.O., Bronson,R.T., Donahue,L.R. and Davisson,M.T.
  TITLE     Mesenchymal dysplasia: a recessive mutation on chromosome 13 of the
            mouse
  JOURNAL   J Hered 87 (2), 87-95 (1996)
   PUBMED   8830098
REFERENCE   10 (bases 1 to 7318)
  AUTHORS   Goodrich,L.V., Johnson,R.L., Milenkovic,L., McMahon,J.A. and
            Scott,M.P.
  TITLE     Conservation of the hedgehog/patched signaling pathway from flies
            to mice: induction of a mouse patched gene by Hedgehog
  JOURNAL   Genes Dev 10 (3), 301-312 (1996)
   PUBMED   8595881
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147626.1, AB164615.1, CN535675.1 and BU700582.1.
            
            Transcript Variant: This variant (2) contains an alternate 5'
            terminal exon and thus differs in the 5' UTR and 5' coding region,
            compared to variant 1. The encoded isoform (b) has a distinct
            N-terminus and is shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164139
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-961               AK147626.1         1-961
            962-1143            AB164615.1         668-849
            1144-2052           AK147626.1         1143-2051
            2053-2114           CN535675.1         390-451
            2115-4516           AK147626.1         2052-4453
            4517-7045           AK147626.1         4873-7401
            7046-7318           BU700582.1         1-273               c
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 32.8 cM"
     gene            1..7318
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /note="patched 1"
                     /db_xref="GeneID:19206"
                     /db_xref="MGI:MGI:105373"
     exon            1..369
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     CDS             367..4515
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /note="isoform b is encoded by transcript variant 2;
                     protein patched homolog 1; Ptch1 protein +12b; mesenchymal
                     dysplasia"
                     /codon_start=1
                     /product="protein patched homolog 1 isoform b"
                     /protein_id="NP_001315443.1"
                     /db_xref="GeneID:19206"
                     /db_xref="MGI:MGI:105373"
                     /translation="MGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFG
                     AFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGAN
                     VLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPC
                     LIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNK
                     AEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELI
                     VGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQ
                     RTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTML
                     RWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVF
                     LLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFS
                     LQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTE
                     PHSNTRYSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPV
                     TVTQDNLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPK
                     AKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIV
                     TQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWE
                     TGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIY
                     LTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNG
                     LRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLAC
                     TFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEF
                     TVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFA
                     VLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPG
                     HTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVV
                     HPDSRHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTE
                     GHSGPSNRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPG
                     PGRNPRGGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWG
                     SSSN"
     exon            370..562
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            563..752
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            753..822
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            823..914
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1113
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1235
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1383
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1515
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1671
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1770
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1896
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..2015
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2418
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2728
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            2729..2871
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            2872..3055
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3336
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3337..3474
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3475..3617
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3618..3717
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3718..3972
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            3973..4516
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     exon            4517..7301
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7274..7279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /note="hexamer: AATAAA"
     polyA_site      7301
                     /gene="Ptch1"
                     /gene_synonym="A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig"
                     /note="major polyA site"
ORIGIN      
        1 gagggccatg gacctgctta atagaaacag gcttgtaatt gtgagtccgc gccgcactcc
       61 gccgaaagcc tgcggcggcc ccgcgcgccg gggtttttac actttccgtt ccttttgtaa
      121 agacggagga ggaggagaag acgaagacga agacgggggg ggggggaaaa gagaaggcga
      181 cagggcagac aaagagaccc gcggcgacag ggcctggggg agaagaagag agacagggag
      241 aagagagagc cacgcagcac cagccaaggg gggccccggg aaattaataa aaggaattga
      301 tgtgaacctc acggtcagcg tccgcgccat cggcagctgc gctccggcca ggggctgagg
      361 ccggctatgg ggaaggctac tggccggaaa gcgccgctgt ggctgagagc gaagtttcag
      421 agactcttat ttaaactggg ttgttacatt caaaagaact gcggcaagtt tttggttgtg
      481 ggtctcctca tatttggggc cttcgctgtg ggattaaagg cagctaatct cgagaccaac
      541 gtggaggagc tgtgggtgga agttggtgga cgagtgagtc gagaattaaa ttatacccgt
      601 cagaagatag gagaagaggc tatgtttaat cctcaactca tgatacagac tccaaaagaa
      661 gaaggcgcta atgttctgac cacagaggct ctcctgcaac acctggactc agcactccag
      721 gccagtcgtg tgcacgtcta catgtataac aggcaatgga agttggaaca tttgtgctac
      781 aaatcagggg aacttatcac ggagacaggt tacatggatc agataataga atacctttac
      841 ccttgcttaa tcattacacc tttggactgc ttctgggaag gggcaaagct acagtccggg
      901 acagcatacc tcctaggtaa gcctccttta cggtggacaa actttgaccc cttggaattc
      961 ctagaagagt taaagaaaat aaactaccaa gtggacagct gggaggaaat gctgaataaa
     1021 gccgaagttg gccatgggta catggaccgg ccttgcctca acccagccga cccagattgc
     1081 cctgccacag cccctaacaa aaattcaacc aaacctcttg atgtggccct tgttttgaat
     1141 ggtggatgtc aaggtttatc caggaagtat atgcattggc aggaggagtt gattgtgggt
     1201 ggtaccgtca agaatgccac tggaaaactt gtcagcgctc acgccctgca aaccatgttc
     1261 cagttaatga ctcccaagca aatgtatgaa cacttcaggg gctacgacta tgtctctcac
     1321 atcaactgga atgaagacag ggcagccgcc atcctggagg cctggcagag gacttacgtg
     1381 gaggtggttc atcaaagtgt cgccccaaac tccactcaaa aggtgcttcc cttcacaacc
     1441 acgaccctgg acgacatcct aaaatccttc tctgatgtca gtgtcatccg agtggccagc
     1501 ggctacctac tgatgcttgc ctatgcctgt ttaaccatgc tgcgctggga ctgctccaag
     1561 tcccagggtg ccgtggggct ggctggcgtc ctgttggttg cgctgtcagt ggctgcagga
     1621 ttgggcctct gctccttgat tggcatttct tttaatgctg cgacaactca ggttttgccg
     1681 tttcttgctc ttggtgttgg tgtggatgat gtcttcctcc tggcccatgc attcagtgaa
     1741 acaggacaga ataagaggat tccatttgag gacaggactg gggagtgcct caagcgcacc
     1801 ggagccagcg tggccctcac ctccatcagc aatgtcaccg ccttcttcat ggccgcattg
     1861 atccctatcc ctgccctgcg agcgttctcc ctccaggctg ctgtggtggt ggtattcaat
     1921 tttgctatgg ttctgctcat ttttcctgca attctcagca tggatttata cagacgtgag
     1981 gacagaagat tggatatttt ctgctgtttc acaagcccct gtgtcagcag ggtgattcaa
     2041 gttgagccac aggcctacac agagcctcac agtaacaccc ggtacagccc cccaccccca
     2101 tacaccagcc acagcttcgc ccacgaaacc catatcacta tgcagtccac cgttcagctc
     2161 cgcacagagt atgaccctca cacgcacgtg tactacacca ccgccgagcc acgctctgag
     2221 atctctgtac agcctgttac cgtcacccag gacaacctca gctgtcagag tcccgagagc
     2281 accagctcta ccagggacct gctctcccag ttctcagact ccagcctcca ctgcctcgag
     2341 cccccctgca ccaagtggac actctcttcg tttgcagaga agcactatgc tcctttcctc
     2401 ctgaaaccca aagccaaggt tgtggtaatc cttcttttcc tgggcttgct gggggtcagc
     2461 ctttatggga ccacccgagt gagagacggg ctggacctca cggacattgt tccccgggaa
     2521 accagagaat atgacttcat agctgcccag ttcaagtact tctctttcta caacatgtat
     2581 atagtcaccc agaaagcaga ctacccgaat atccagcacc tactttacga ccttcataag
     2641 agtttcagca atgtgaagta tgtcatgctg gaggagaaca agcaacttcc ccaaatgtgg
     2701 ctgcactact ttagagactg gcttcaagga cttcaggatg catttgacag tgactgggaa
     2761 actgggagga tcatgccaaa caattataaa aatggatcag atgacggggt cctcgcttac
     2821 aaactcctgg tgcagactgg cagccgagac aagcccatcg acattagtca gttgactaaa
     2881 cagcgtctgg tagacgcaga tggcatcatt aatccgagcg ctttctacat ctacctgacc
     2941 gcttgggtca gcaacgaccc tgtagcttac gctgcctccc aggccaacat ccggcctcac
     3001 cggccggagt gggtccatga caaagccgac tacatgccag agaccaggct gagaatccca
     3061 gcagcagagc ccatcgagta cgctcagttc cctttctacc tcaacggcct acgagacacc
     3121 tcagactttg tggaagccat agaaaaagtg agagtcatct gtaacaacta tacgagcctg
     3181 ggactgtcca gctaccccaa tggctacccc ttcctgttct gggagcaata catcagcctg
     3241 cgccactggc tgctgctatc catcagcgtg gtgctggcct gcacgtttct agtgtgcgca
     3301 gtcttcctcc tgaacccctg gacggccggg atcattgtca tggtcctggc tctgatgacc
     3361 gttgagctct ttggcatgat gggcctcatt gggatcaagc tgagtgctgt gcctgtggtc
     3421 atcctgattg catctgttgg catcggagtg gagttcaccg tccacgtggc tttggccttt
     3481 ctgacagcca ttggggacaa gaaccacagg gctatgctcg ctctggagca catgtttgct
     3541 cccgttctgg acggtgctgt gtccactctg ctgggtgtac tgatgcttgc agggtccgaa
     3601 tttgatttca ttgtcagata cttctttgcc gtcctggcca ttctcaccgt cttgggggtt
     3661 ctcaatggac tggttctgct gcctgtcctc ttatccttct ttggaccgtg tcctgaggtg
     3721 tctccagcca atggcctaaa ccgactgccc actccttcgc ctgagccgcc tccaagtgtc
     3781 gtccggtttg ccgtgcctcc tggtcacacg aacaatgggt ctgattcctc cgactcggag
     3841 tacagctctc agaccacggt gtctggcatc agtgaggagc tcaggcaata cgaagcacag
     3901 cagggtgccg gaggccctgc ccaccaagtg attgtggaag ccacagaaaa ccctgtcttt
     3961 gcccggtcca ctgtggtcca tccggactcc agacatcagc ctcccttgac ccctcggcaa
     4021 cagccccacc tggactctgg ctccttgtcc cctggacggc aaggccagca gcctcgaagg
     4081 gatcccccta gagaaggctt gcggccaccc ccctacagac cgcgcagaga cgcttttgaa
     4141 atttctactg aagggcattc tggccctagc aatagggacc gctcagggcc ccgtggggcc
     4201 cgttctcaca accctcggaa cccaacgtcc accgccatgg gcagctctgt gcccagctac
     4261 tgccagccca tcaccactgt gacggcttct gcttcggtga ctgttgctgt gcatcccccg
     4321 cctggacctg ggcgcaaccc ccgagggggg ccctgtccag gctatgagag ctaccctgag
     4381 actgatcacg gggtatttga ggatcctcat gtgccttttc atgtcaggtg tgagaggagg
     4441 gactcaaagg tggaggtcat agagctacag gacgtggaat gtgaggagag gccgtggggg
     4501 agcagctcca actgagggta attaaaatct gaagcaaaga ggccaaagat tggaaagccc
     4561 cgcccccacc tctttccaga actgcttgaa gagaactgct tggaattatg ggaaggcagt
     4621 tcattgttac tgtaactgat tgtattattt tgtgaaatat ttctataaat atttaaaagg
     4681 tgtacacatg taatatacat ggaaatgctg tacagtctat ttcctggggc ctctccactc
     4741 ctgccccaga gtggggagac cacaggggcc ctttcccctg tgtacattgg tctctgtgcc
     4801 acaaccaagc ttaacttagt tttaaaaaaa atctcccagc atatgtcgct gctgcttaaa
     4861 tattgtataa tttacttgta taattctatg caaatattgc ttatgtaata ggattatttg
     4921 taaaggtttc tgtttaaaat attttaaatt tgcatatcac aaccctgtgg taggatgaat
     4981 tgttactgtt aacttttgaa cacgctatgc gtggtaattg tttaacgagc agacatgaag
     5041 aaaacaggtt aatcccagtg gcttctctag gggtagttgt atatggttcg catgggtgga
     5101 tgtgtgtgtg catgtgactt tccaatgtac tgtattgtgg tttgttgttg ttgttgctgt
     5161 tgttgttcat ttttgtgttt tttgttgctt tgtatgatct tagctctggc ctaggtgggc
     5221 tgggaaggtc caggtctttt tctgtcgtga tgctggtgga aaggtgaccc caatcatctg
     5281 tcctattctc tgggactatt caagagaagc cagatttgct tcatgcctgt gtgtggcagc
     5341 ttctgaggtc atgggtagcc ctccaagcac ctctgcttgg gtttcaaaga gaagatgttc
     5401 tcacaaacat tgtgctgcct attagggcct ctatatagtc agcagtcagc actagtggtg
     5461 aaagattgga caatgttgcc tgatggtgag actcagccgg gtcatgagat taccctctag
     5521 gggtgtgtgg ttgttccttt ctgatgatca cacatacaca cagcctccca cccccatact
     5581 ccagatacag gcccaaaaaa acctatttca ctgaaggtct atattcgagc ctttacaaat
     5641 gatagtccct cccttgctat gactgcctgg ttggagcagg ctaggctaat cgtggttcag
     5701 tcaactgtgt ggggagagtt tctaaggact cttttccaca cggtcttttc ctgtgtatac
     5761 tttcttctct tccccactgt tagcatccat ccagtgtgat gtctgtgaaa gcaggaagat
     5821 gccacctcgt aaaagcatat agggtaagct ctttgaacca ggtgttgggc tttagagttt
     5881 gattgtgttc tccttcccca aggtttgggt ttctttgtct ctggtcagca actagtagat
     5941 agggaaggaa atgcccttat tccttgcaag tgaacattga ggacttgtct cagaggaagg
     6001 aagcctagtg ctcatggaag ctgttgtgag gaatgtactc tgtctgcatg cagcttccct
     6061 gaatccctcc tatccttcat ggaaaaattt aaggagatgg gtccctagta gtaccaataa
     6121 tctagtgccc tcagatccta aaatttatcc cccaaattgt aaggtactaa ttggaagcca
     6181 cctgtgacta ccgtgtgtgt gtgtgtctgt gtctgtctgt atatccgtgt gcgcgtgcgc
     6241 gccagaagcc ctggccatcc actgctactg tcttaatgcc tgtcaagcca ctgtctgcct
     6301 acccaacacc agctctgctc ggatatcttg caccctgagt tgaggaggga gatgtagagt
     6361 gcggaagcca ccttggctgt ggttcttgat tgtgtccctc atgcctgagc cttgtgcatg
     6421 tggcagaagg aagtttgtac agcctctcgg cttctgtgca tcatcatgag tcccatcagc
     6481 caaggcagga aggacacaga gctggcagga gactaaagtc agagagtgtg tgtctctgtc
     6541 tgtctctctc atagttttat tttgtctgta ttgtttgttc atttggatgt tttaatttgt
     6601 aaaagaaaag atctttgctg atatttataa ttttgtatca taagaatgtc ctccagaatt
     6661 tgtcatgcca gtttataaca agaagaaaaa attgcaggga ttttatttct attggaaaca
     6721 cttattgcag ttatgtttta cttttgaaca gaagttttat ttgtatagag tgcttactaa
     6781 tgttaaatag ttcagagtat ataacattta cattaaggac tcatggtagg ttttagtgta
     6841 aggagtttaa aggaaataaa tattcaaact gggtctcgtc tgccaatttg ggtggaaatg
     6901 agtttgtgtc acttcaatta caaagatgaa agtatgccat ataatttatt tatatgaaaa
     6961 tttatttttg tagtgtacat agtagtcatc aagtctttcg acagaagtat atttttaaag
     7021 aatttatatg tgatgaaatc cataatgtct ggaactttgc tgagacatgg gtgtgaggac
     7081 acgtttcgtt ataaatgaca gcaagggaga gaagagagta tgttttaaca gtgttaggag
     7141 agtacacgtg agcagtgatc catgtgattg gaaagtatcg gtgtgaacat ggtgacctag
     7201 tgcggttctc agatgaaaat gtacaaaact ctctaaatat taatgttcaa acactgatag
     7261 aaattctaac atgaataaag ataatataac ttgttggttt aaaaaaaaaa aaaaaaaa
//
