LOCUS       NM_212446               3559 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus proteasome (prosome, macropain) inhibitor subunit 1
            (Psmf1), transcript variant 1, mRNA.
ACCESSION   NM_212446
VERSION     NM_212446.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Minis,A., Rodriguez,J.A., Levin,A., Liu,K., Govek,E.E., Hatten,M.E.
            and Steller,H.
  TITLE     The proteasome regulator PI31 is required for protein homeostasis,
            synapse maintenance, and neuronal survival in mice
  JOURNAL   Proc Natl Acad Sci U S A 116 (49), 24639-24650 (2019)
   PUBMED   31754024
  REMARK    GeneRIF: Study in global and conditional PI31 knockout mouse
            establishes a critical role for PI31 and protein degradation in the
            maintenance of neuronal architecture, circuitry, and function.
            Because mutations that impair PI31 function are thought to cause
            neurodegenerative diseases in humans, reduced PI31 activity may
            contribute to age-related neurodegenerative diseases.
REFERENCE   3  (bases 1 to 3559)
  AUTHORS   Liu,K., Jones,S., Minis,A., Rodriguez,J., Molina,H. and Steller,H.
  TITLE     PI31 Is an Adaptor Protein for Proteasome Transport in Axons and
            Required for Synaptic Development
  JOURNAL   Dev Cell 50 (4), 509-524 (2019)
   PUBMED   31327739
  REMARK    GeneRIF: Inactivation of PI31 in mouse neurons attenuated
            proteasome movement in axons, indicating this process is conserved.
            Because mutations affecting PI31 activity are associated with human
            neurodegenerative diseases, impairment of PI31-mediated axonal
            transport of proteasomes may contribute to these disorders.
REFERENCE   4  (bases 1 to 3559)
  AUTHORS   Alvarez-Dominguez,J.R., Zhang,X. and Hu,W.
  TITLE     Widespread and dynamic translational control of red blood cell
            development
  JOURNAL   Blood 129 (5), 619-629 (2017)
   PUBMED   27899360
REFERENCE   5  (bases 1 to 3559)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 3559)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 3559)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 3559)
  AUTHORS   Zaiss,D.M., Standera,S., Kloetzel,P.M. and Sijts,A.J.
  TITLE     PI31 is a modulator of proteasome formation and antigen processing
  JOURNAL   Proc Natl Acad Sci U S A 99 (22), 14344-14349 (2002)
   PUBMED   12374861
  REMARK    GeneRIF: PI31 acts as a selective modulator of the
            proteasome-mediated steps in MHC class I antigen processing
REFERENCE   9  (bases 1 to 3559)
  AUTHORS   Zaiss,D.M., Standera,S., Holzhutter,H., Kloetzel,P. and Sijts,A.J.
  TITLE     The proteasome inhibitor PI31 competes with PA28 for binding to 20S
            proteasomes
  JOURNAL   FEBS Lett 457 (3), 333-338 (1999)
   PUBMED   10471803
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BE649179.1, AK053924.1 and AK083431.1.
            
            On Mar 5, 2015 this sequence version replaced NM_212446.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.208633.1, AK031363.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                BE649179.1         1-17
            18-3391             AK053924.1         4-3377
            3392-3559           AK083431.1         3141-3308
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 74.83 cM"
     gene            1..3559
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /note="proteasome (prosome, macropain) inhibitor subunit
                     1"
                     /db_xref="GeneID:228769"
                     /db_xref="MGI:MGI:1346072"
     exon            1..300
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     CDS             172..987
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proteasome inhibitor PI31 subunit"
                     /codon_start=1
                     /product="proteasome inhibitor PI31 subunit isoform 1"
                     /protein_id="NP_997611.1"
                     /db_xref="CCDS:CCDS16872.1"
                     /db_xref="GeneID:228769"
                     /db_xref="MGI:MGI:1346072"
                     /translation="MAGLEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQP
                     GPSDKKSELLPAKWNSNKELYALRYESKDGARKLLLKAVSVENGMIINVLELGTQQVA
                     DLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIITPIHEQWEKARANSPPREFPP
                     ATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIVDPLRSGFP
                     RVLIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPSGPNPDHLPPPGYDDMYL"
     exon            301..453
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            454..536
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            537..722
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            723..776
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            777..935
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            936..3559
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1337..1342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /note="hexamer: AATACA"
     polyA_site      1360
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /note="major polyA site"
     regulatory      2856..2861
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Psmf1"
                     /gene_synonym="PI31"
                     /note="hexamer: AATAAA"
     polyA_site      2878
                     /gene="Psmf1"
                     /gene_synonym="PI31"
     polyA_site      3291
                     /gene="Psmf1"
                     /gene_synonym="PI31"
     polyA_site      3394
                     /gene="Psmf1"
                     /gene_synonym="PI31"
ORIGIN      
        1 cgtctctgcg tttccgcgtt tccacttttc cgcgcaggtg cggacagagc cgaaagcgaa
       61 ggcggcatca aaaggcgtcg ctgccgctcg cggtcccgct tctcttaggc ggagcggctt
      121 tctgcatagt cccctccttt tcttcctttc ccacagaagc cgcaaacatc catggcgggt
      181 ctcgaggttc tcttcgcgtc ggccgctcca tccatgagct gcccgcagga cgcactcgtc
      241 tgcttcctgc actgggaagt ggtgacaaac ggctactatg ccttgggcac gggcgaccag
      301 ccaggtccca gtgataagaa atcagaacta ctgccagcta agtggaacag caataaagaa
      361 ctgtatgccc tccggtatga gtctaaggat ggagccagaa aactccttct gaaagctgtc
      421 tctgtggaga acggcatgat catcaacgtg ctggaacttg gcacacagca ggtggcagac
      481 ttgaccctga acttagatga ttatattgat gcagaagacc tgagtgactt ccacaggacc
      541 tataagaaca gtgaggagct tcggtctcag atcagatctg gaattatcac acctatccat
      601 gagcagtggg agaaggctcg tgcaaacagt cctccgaggg agttcccacc tgccactgcc
      661 agagaggtgg acccactcca gatttcctca caccgtcctc atactagcag gcagcctgct
      721 tggcgtgatc ccctgagccc atttgctgtc gggggagatg acctagaccc ttttgggtgt
      781 cagagaggtg gcatgattgt ggatcccctg agatccggct tcccaagagt acttattgac
      841 ccatcctcag gcctcccaaa ccggcttcct cctggtgctg tgcccccagg agctcgcttt
      901 gacccctttg gacccattgg gaccagccca tctggaccta accctgacca tcttccccca
      961 ccgggctacg atgacatgta cctgtgaagg cctcaagaat gtaacagctc agcctccctt
     1021 ttactctcca ggagctgcca ccactggccc catcagcgtc agtattcttg tgggcttggg
     1081 gcagaggatt ctgcccatgt gtttgcaggc ctgctggggt tgcatcccca gcccttgtca
     1141 gagccgaggt gcctgctgca gcaccccacc aagctgcata taggtcccac ggagaaatta
     1201 gtgtgtctca atcaccagct cctccccact tgcaaggaag actccggcaa catcagccct
     1261 ccacccgatg cagtcgcggt cacagcgcta taagaacaga acgcattttg gatgttatta
     1321 ttaagaacca aatgtcaata cagaatacat gttgccagtc ttccacgctc ctttttgtta
     1381 atgcacaaac tcttaagagt gtgatactgc ataggactac gggattccta cagggcattc
     1441 ttctcaagca ccctcgagtc acaagttact cttgctcctg acatcactaa gatgagcccc
     1501 acccaggctt tctgggtgga agtgtgattg gaatccatga gccacaagga tgatttcacc
     1561 aaagcaggca cactagaaaa actgccatct cctcccaagg gcatgctcaa ccagttgctc
     1621 tgagcagcct gttgactctc cctgggctgc agtggcctgt aagtagagga ataaacagaa
     1681 cagacctgta ggctagtttc atagtgaccc taaagaagtc ctttctctta ggagcctagt
     1741 aacgacctgt gaaatcagag gtgccttcac tggagctctg tagtgagctg tgtggatggg
     1801 aatagcccta ggtactagag aagagaaaac gaaggtacgc tgtgtctatt acctgctctt
     1861 catgtcctcc tgggactgac aagagcatag gaacatgggt ccagccagga ggaaggggag
     1921 cctttgtctt tggaagcctg tgtccaagct ctcccaatct gtagaattat ctagatattt
     1981 gaagccccag ctgacaggca ctggcagtgc tctacccctg tgccataaag ctggaagagc
     2041 acaatggcgt actgttccag gagacaccaa atcaagtacc aagcgcttcg ttccatggcc
     2101 ggcagcccaa ccatgtgtgg ctccaggata gatggggaga tagactggac tgacaggacc
     2161 agggccagtc tcagggaggc tgggcttggg gctcaatcca gcatgagcag gctccctagc
     2221 tgttgaagga tcccatgggt caggcactga ccaccttgaa ggtgtaggcc attctgtgct
     2281 ctgaggccac agaccatgca gagacaagga caagacgtcc attgtgcatc aacttcatcc
     2341 ttgagggctg gcaggtggat gtttgggttc tgttggtaac tgggtacctg caaaagagct
     2401 taggacactt tgctcattaa agcatccctt ttttatgcaa ggaagcatta aaggaacagt
     2461 ggctagaatt ctccagatag gtttgggaat gtagataact tggaccctga tttaaaggaa
     2521 aaggtaaaag ttgaaacatt cttccattgg gctttttagt agcaaagata aaaaatataa
     2581 tcatacatta gttctgtttt ctacagacaa actgcagtgt ttctggagaa aggactggtt
     2641 tggtttggtt cctataagtc ttatatagta tctctaagaa acccagtatc atagatgtgg
     2701 gaagccttga atggtgcctc caggaacttg tcaagcagct ggaacttgtc tcagtctctg
     2761 tcacttaacc tcctcccagc tgtgtacggc caacagcaag agggaatgtt tcagtgtatg
     2821 tcccatagat cctccagact ggagcctttg cagtaaataa atagcacttt ataacctgtt
     2881 ttttttaata gctttattaa gagaatttac ataaccatga aattcactat tttaaagtac
     2941 acaactcagt gggttgggag atattcacag tgctgtgcat ccaaccccag attcctccaa
     3001 aagaaatatc ttcactggca atcactccca ttttccctcc cctgggcagg caaccctcca
     3061 gtctgtttgc ctattttgga tagttttcat aagtgacatc actcagtgtg tggtctgtgt
     3121 gtccagcatt tcccactctc tgtcctcact gttcatgtga ttgtagcatc gacattcccc
     3181 atggctgtac aatcatagca tggctatgtc atatttgtta atttataagt tcatggatgc
     3241 ttggattgtt tctccttgct tgttatattg aatgctgtta acattcatgt acaaagtggg
     3301 ttttgttatt ttgcgtgggc agtttttaag tcattgaatg tatctggaat ggattttcag
     3361 ggtcccataa taacacgtta aattttttga ggaactgcca gtgtttccca aaacagccac
     3421 gctattttca taccagaggt agtgctccag tttctctgta tcctcaccaa tccttgcttg
     3481 tacccaaact agtgatgtca agtcatatct tgtttgtatt gtcccatttg cttttgtttt
     3541 ttaatggaaa aatttgacc
//
