LOCUS       NM_080554               2065 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus proteasome (prosome, macropain) 26S subunit,
            non-ATPase, 5 (Psmd5), transcript variant 1, mRNA.
ACCESSION   NM_080554
VERSION     NM_080554.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Levin,A., Minis,A., Lalazar,G., Rodriguez,J. and Steller,H.
  TITLE     PSMD5 Inactivation Promotes 26S Proteasome Assembly during
            Colorectal Tumor Progression
  JOURNAL   Cancer Res 78 (13), 3458-3468 (2018)
   PUBMED   29716915
  REMARK    GeneRIF: Study in HT-29 cells and mouse colorectal tumor models
            revealed that levels of the proteasome assembly inhibitor PSMD5/S5B
            are significantly decreased in tumors, and re-expression of PSMD5
            blocks 26S proteasome assembly. These results suggest that
            intestinal cancer cells escape proteotoxic stress by reducing PSMD5
            to stimulate 26S proteasome assembly.
REFERENCE   3  (bases 1 to 2065)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2065)
  AUTHORS   Shim,S.M., Lee,W.J., Kim,Y., Chang,J.W., Song,S. and Jung,Y.K.
  TITLE     Role of S5b/PSMD5 in proteasome inhibition caused by
            TNF-alpha/NFkappaB in higher eukaryotes
  JOURNAL   Cell Rep 2 (3), 603-615 (2012)
   PUBMED   22921402
  REMARK    GeneRIF: the critical role of S5b/PSMD5 in negative regulation of
            proteasome by TNF-alpha/NFkappaB and provide insights into
            proteasome inhibition in human disease.
REFERENCE   5  (bases 1 to 2065)
  AUTHORS   Gomes,A.V., Zong,C., Edmondson,R.D., Li,X., Stefani,E., Zhang,J.,
            Jones,R.C., Thyparambil,S., Wang,G.W., Qiao,X., Bardag-Gorce,F. and
            Ping,P.
  TITLE     Mapping the murine cardiac 26S proteasome complexes
  JOURNAL   Circ Res 99 (4), 362-371 (2006)
   PUBMED   16857966
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK148284.1, AK032992.1 and BY449619.1.
            
            On Mar 24, 2007 this sequence version replaced NM_080554.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148284.1, SRR1660817.8827.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AK148284.1         2-24
            24-2042             AK032992.1         2-2020
            2043-2065           BY449619.1         376-398
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.99 cM"
     gene            1..2065
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /note="proteasome (prosome, macropain) 26S subunit,
                     non-ATPase, 5"
                     /db_xref="GeneID:66998"
                     /db_xref="MGI:MGI:1914248"
     exon            1..213
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..1555
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /note="isoform 1 is encoded by transcript variant 1; 26S
                     proteasome non-ATPase regulatory subunit 5; 26S proteasome
                     subunit S5B; 26S protease subunit S5 basic"
                     /codon_start=1
                     /product="26S proteasome non-ATPase regulatory subunit 5
                     isoform 1"
                     /protein_id="NP_542121.2"
                     /db_xref="CCDS:CCDS15953.1"
                     /db_xref="GeneID:66998"
                     /db_xref="MGI:MGI:1914248"
                     /translation="MAAQAVSLLREVARLEAPLEELRALQSVVQAVPLHELREQAAEL
                     RLRPLFSLLNQNNREQTALCVSILERLLQAVEPIHLARNLRLDLQRGLTHPDDSVKTL
                     TLSQIGRIVENSEAVTEILNNAELLKQIVYCIGGENLSVAKAAIKSLSRISLTQAGLE
                     ALFESNLLDDLKNVMKTNDVVRYRVYELIIDISSVSSESLNYCTTSGLVTQLLKELTG
                     EDVLVRATCIEMVTSLAYTHHGRQYLAQEGVIDQISNIIVGADSDPFSGFYLPGFVKF
                     FGNLAVMDSPQQICERYPVFLEKVFEMADSQDPTMIGVAVDTVGILGSSVEGKQVLQK
                     TGTRFERVLMRVGYQAKNASTELKIRCLDAVSSLLYLSPEQQTDDFLGMTESWFSSMS
                     RDSLELFRGISNQPFPELHCAALKVFTAIADQPWAQRLMFNSPGFVEFVMDRSVEHDK
                     ASKDAKYELVKALANSKTVAEIFGNSNYLRLRAYLSEGPYYVKPVATTAVEGAD"
     exon            214..358
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     exon            359..472
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     exon            473..601
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     exon            602..711
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     exon            712..854
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1046
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1156
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1297
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..2042
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1823..1828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /note="hexamer: AATAAA"
     polyA_site      1843
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /note="major polyA site"
     regulatory      2016..2021
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /note="hexamer: AATAAA"
     polyA_site      2031
                     /gene="Psmd5"
                     /gene_synonym="1500032A03Rik; mKIAA0072; S5b"
                     /note="major polyA site"
ORIGIN      
        1 gggaacctag taggtgggcg ggcagacggg agccagggag atggcggcgc aggctgtgtc
       61 gctcctgcgg gaggtggcgc ggctggaggc gccgctggag gagcttcgtg cgctgcagtc
      121 agtggtgcag gctgtgccgc tgcacgagct tcgcgagcag gcggcggagc tacgcctccg
      181 cccgctgttc tccttgctta accagaacaa tcgggaacag actgctttgt gtgtctccat
      241 tctggagagg ctgctccaag ctgtggagcc gattcacttg gccaggaacc tcaggcttga
      301 cctgcagagg ggactgactc accctgatga ctctgtaaaa accctcactc tgtcgcagat
      361 tggaagaatt gttgaaaatt cagaagctgt tactgagatt ctcaataatg ctgagctgtt
      421 gaaacagatt gtttattgca ttggcggaga gaatttatct gttgctaaag cggctattaa
      481 atccctgtcg agaatatcac taacccaggc tggcctggaa gctctgtttg aaagtaacct
      541 gctggatgat ttgaagaatg taatgaaaac aaacgacgtt gttcgataca gggtctatga
      601 gctaattata gatatttctt ctgtgtcatc agaatcttta aactactgta ccacaagtgg
      661 actggtgact caactcctaa aagagctgac gggtgaggac gtgttagtca gagccacctg
      721 tatagaaatg gtgacatcac tagcttatac ccatcacgga cgacaatacc ttgctcaaga
      781 aggagtcatt gaccagatat ccaatataat tgttggcgca gattcagacc ctttctctgg
      841 cttctatctg ccaggatttg tgaagttttt tggaaacctg gccgtcatgg atagtcctca
      901 gcagatctgt gagcgctacc ctgtttttct ggagaaggtg tttgaaatgg cggatagtca
      961 agaccccacc atgattggtg tcgctgtcga cacagttgga atcctgggat ccagtgttga
     1021 aggaaaacaa gttttacaga agacaggaac ccgctttgaa cgtgtcctca tgagagtagg
     1081 atatcaagca aagaatgctt ccacggagct gaagattagg tgcttggatg cagtttcctc
     1141 tctcctgtat ttatcgcctg agcagcagac tgatgacttc ctgggaatga cagaatcctg
     1201 gttttcctcc atgtctcgag actccctgga gcttttccgt ggtatcagta accagccctt
     1261 cccggagcta cactgtgctg ccttgaaagt tttcacagct attgcagacc agccctgggc
     1321 tcagagactt atgtttaaca gtccaggttt tgtagagttt gtgatggacc ggtctgtgga
     1381 acatgacaaa gcttcaaagg atgccaaata tgaactggta aaagcacttg ccaattccaa
     1441 gacagttgca gagatctttg ggaactcaaa ttatttgagg ctcagagcgt acctaagtga
     1501 aggtccatac tatgtgaaac ctgttgccac aacagcagta gaaggggctg actgatccct
     1561 ttccacctaa aacagcagaa tttcccaagg cacacgattc catccgtgct ccacaccaca
     1621 ggacgtctgt cttcccaggc acagcctcac catcagcagt gttgtcccta cttaatgctg
     1681 tgtagactcg gctcttgtaa aagcagtagg agcctgagag tgctcgtgtt tccccgagtc
     1741 ctctggctgc caaagtctct gctgcaaggt cgaggtttgt tttctcaaag tttccatggg
     1801 cacactcatg acatttttaa aaaataaaat gaagccaaaa tgattctgga ttctctcaac
     1861 cacattttct ttttccttac ctgatttttg cctcaaaatc ttgggtatct tgggtagtct
     1921 ctaaaaaata agtcatttag aatcatcgaa ttatattatt tgtatataag gaataatagt
     1981 ttgaataata tttcttactt ttcctttgaa aataaaataa atccaaactt gtttggcgtt
     2041 ggaaaaaaaa aaaaaaaaaa aaaaa
//
