LOCUS       NM_001163609            1693 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus proteasome subunit alpha 8 (Psma8), transcript variant
            1, mRNA.
ACCESSION   NM_001163609 XM_357002 XM_906754
VERSION     NM_001163609.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Zhang,Z.H., Jiang,T.X., Chen,L.B., Zhou,W., Liu,Y., Gao,F. and
            Qiu,X.B.
  TITLE     Proteasome subunit alpha4s is essential for formation of
            spermatoproteasomes and histone degradation during meiotic DNA
            repair in spermatocytes
  JOURNAL   J Biol Chem 296, 100130 (2021)
   PUBMED   33262216
REFERENCE   3  (bases 1 to 1693)
  AUTHORS   Felipe-Medina,N., Caburet,S., Sanchez-Saez,F., Condezo,Y.B., de
            Rooij,D.G., Gomez-H,L., Garcia-Valiente,R., Todeschini,A.L.,
            Duque,P., Sanchez-Martin,M.A., Shalev,S.A., Llano,E., Veitia,R.A.
            and Pendas,A.M.
  TITLE     A missense in HSF2BP causing primary ovarian insufficiency affects
            meiotic recombination by its novel interactor C19ORF57/BRME1
  JOURNAL   Elife 9, e56996 (2020)
   PUBMED   32845237
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1693)
  AUTHORS   Kondo,H., Matsumura,T., Kaneko,M., Inoue,K., Kosako,H., Ikawa,M.,
            Takahama,Y. and Ohigashi,I.
  TITLE     PITHD1 is a proteasome-interacting protein essential for male
            fertilization
  JOURNAL   J Biol Chem 295 (6), 1658-1672 (2020)
   PUBMED   31915251
REFERENCE   5  (bases 1 to 1693)
  AUTHORS   Gomez-H,L., Felipe-Medina,N., Condezo,Y.B., Garcia-Valiente,R.,
            Ramos,I., Suja,J.A., Barbero,J.L., Roig,I., Sanchez-Martin,M., de
            Rooij,D.G., Llano,E. and Pendas,A.M.
  TITLE     The PSMA8 subunit of the spermatoproteasome is essential for proper
            meiotic exit and mouse fertility
  JOURNAL   PLoS Genet 15 (8), e1008316 (2019)
   PUBMED   31437213
  REMARK    GeneRIF: Study in mouse model shows that Psma8 is localized to and
            dependent on the central element of the synaptonemal complex and
            promotes the assembly of the proteasome activator PA200.
            Accordingly, synapsis-deficient mice show delocalization of Psma8.
            Loss of Psma8 leads to infertility in males.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1693)
  AUTHORS   Zhang,Q., Ji,S.Y., Busayavalasa,K., Shao,J. and Yu,C.
  TITLE     Meiosis I progression in spermatogenesis requires a type of
            testis-specific 20S core proteasome
  JOURNAL   Nat Commun 10 (1), 3387 (2019)
   PUBMED   31358751
  REMARK    GeneRIF: meiosis I progression in spermatogenesis, particularly
            entry into and exit from M-phase, requires the proteasomal activity
            of proteasome subunit alpha 8 (PSMA8)-associated proteasomes.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1693)
  AUTHORS   Qian,M.X., Pang,Y., Liu,C.H., Haratake,K., Du,B.Y., Ji,D.Y.,
            Wang,G.F., Zhu,Q.Q., Song,W., Yu,Y., Zhang,X.X., Huang,H.T.,
            Miao,S., Chen,L.B., Zhang,Z.H., Liang,Y.N., Liu,S., Cha,H.,
            Yang,D., Zhai,Y., Komatsu,T., Tsuruta,F., Li,H., Cao,C., Li,W.,
            Li,G.H., Cheng,Y., Chiba,T., Wang,L., Goldberg,A.L., Shen,Y. and
            Qiu,X.B.
  TITLE     Acetylation-mediated proteasomal degradation of core histones
            during DNA repair and spermatogenesis
  JOURNAL   Cell 153 (5), 1012-1024 (2013)
   PUBMED   23706739
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK010717.1.
            
            On or before Jul 26, 2009 this sequence version replaced
            XM_906754.3, XM_357002.5.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK010717.1, BC116990.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1693              AK010717.1         2-1694
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 8.25 cM"
     gene            1..1693
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /note="proteasome subunit alpha 8"
                     /db_xref="GeneID:73677"
                     /db_xref="MGI:MGI:1920927"
     exon            1..182
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..833
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /EC_number="3.4.25.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proteasome alpha 8 subunit; proteasome (prosome,
                     macropain) subunit, alpha type, 8; alpha4s; proteasome
                     alpha 4 subunit; proteasome subunit alpha type-8"
                     /codon_start=1
                     /product="proteasome subunit alpha type-8 isoform 1"
                     /protein_id="NP_001157081.1"
                     /db_xref="CCDS:CCDS50226.1"
                     /db_xref="GeneID:73677"
                     /db_xref="MGI:MGI:1920927"
                     /translation="MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVV
                     LGVEKKSVAKLQDERTVRKICALDDHVCMAFAGLTADARVVISRARVECQSHKLTVED
                     PVTVEYITRFIATLKQKYTQSNGRRPFGISALIVGFDDDGIPRLYQTDPSGTYHAWKA
                     NAIGRSAKTVREFLEKNYTEDAISNDKEAIKLAIKALLEVVQSGGKNIELAIIRRDQP
                     LKMFSAKEIELEVSEIEREKDEAEKTKSKKST"
     exon            183..309
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            310..434
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            435..557
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            558..677
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            678..740
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            741..1693
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1668..1673
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1691
                     /gene="Psma8"
                     /gene_synonym="2410072D24Rik"
                     /note="major polyA site"
ORIGIN      
        1 aggaataacc gcttccgggc gggcgttagc acgtgggggc ccgtggactc ctgtcctagg
       61 cagcttctga ggcctgcgtg atggcttctc ggtatgacag ggcaatcacc gtcttctccc
      121 cagatggaca ccttttccaa gtggagtatg cccaggaagc agtgaagaaa ggctccaccg
      181 cggttggaat tcgaggaact aatatagttg tgcttggggt agaaaaaaag tcagtggcca
      241 agcttcaaga tgaaaggact gtgaggaaaa tctgcgctct cgatgaccat gtctgcatgg
      301 cttttgcagg tctgacagcg gatgctagag tggtgatcag cagagcgcgg gtggagtgcc
      361 agagccacaa gctcacagtg gaggatcccg tcactgtaga gtatataact cgcttcatag
      421 cgacgctaaa gcagaaatat acccagagca atggacgaag gccttttggt atttcagcct
      481 taattgtagg ctttgatgat gatggcatcc caagattgta tcagacagat ccctccggga
      541 cttaccacgc ttggaaagca aatgcaatag gccgaagtgc taaaactgtc cgggaatttc
      601 tggaaaaaaa ttacacagaa gatgcaattt caaatgacaa ggaagctatc aaattagcaa
      661 taaaagcttt gctcgaagtt gtccagtctg gcggaaagaa cattgaactg gctataataa
      721 gaagagatca acctttgaag atgtttagtg ccaaagaaat tgaactggag gtgagtgaaa
      781 tagaaaggga aaaggacgaa gcagagaaga caaagtcaaa gaagtcaacc taatgccagc
      841 gtcactgggc gctgagagtt ccctcctgag gttttagaag aaatcaatgg gttggaacca
      901 accattgagc ttttctacgt tcatcttcaa gaatgtccgt tgttttcaaa cataagttaa
      961 tgggaagatt ttctgtaaaa ttcctcagtt ccacgtaact ctaatcgtat actgtagagc
     1021 ttgaatagaa tagtactgtt gcttttcttc atacaagcaa attctagggg gaaagaagat
     1081 gatttttaaa gttagtaatg ttttgaagtt tgtctcttct ctgttagtta aggagctagt
     1141 catctcagag taaacagaac cctgagcggc atttcttggt gaatggtaag gacatcaatg
     1201 tgttggtgag gtgtcaacaa tttgataaaa tctgtatgtg ctgactccat aggctaaagc
     1261 agaaggattg taagttaggc attgtgatag atgctaaaaa aaatataggg acaaaaataa
     1321 gctcagatta ttggtttttc ttttttaaca taggatctca tgtagcccgc actggccttg
     1381 aaaaccctct gtagccagag gatgaccttg aactcctgat ttttttatct tcacttttca
     1441 atgggtaact cgtctgtccc cccatgccca gctagactta gcttttgtcc ttattactat
     1501 atagcaggaa gatggacatt aagttacaat cattttcatt tgggattaat gtagtgaaat
     1561 tctttatgtc tgccttctat tctaatttag aggctccaat agagctttaa ggctgggttc
     1621 cagatcatac tcatctttgt tcttgcagct tgcctatggt gtgacttaat aaatgtttat
     1681 aaagttggta act
//
