LOCUS       NM_011179               2676 bp    mRNA    linear   ROD 05-FEB-2024
DEFINITION  Mus musculus prosaposin (Psap), transcript variant 2, mRNA.
ACCESSION   NM_011179
VERSION     NM_011179.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Qiu,X., Guo,Y., Liu,M.F., Zhang,B., Li,J., Wei,J.F. and Li,M.
  TITLE     Single-cell RNA-sequencing analysis reveals enhanced non-canonical
            neurotrophic factor signaling in the subacute phase of traumatic
            brain injury
  JOURNAL   CNS Neurosci Ther 29 (11), 3446-3459 (2023)
   PUBMED   37269057
  REMARK    GeneRIF: Single-cell RNA-sequencing analysis reveals enhanced
            non-canonical neurotrophic factor signaling in the subacute phase
            of traumatic brain injury.
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   He,Y., Kaya,I., Shariatgorji,R., Lundkvist,J., Wahlberg,L.U.,
            Nilsson,A., Mamula,D., Kehr,J., Zareba-Paslawska,J., Biverstal,H.,
            Chergui,K., Zhang,X., Andren,P.E. and Svenningsson,P.
  TITLE     Prosaposin maintains lipid homeostasis in dopamine neurons and
            counteracts experimental parkinsonism in rodents
  JOURNAL   Nat Commun 14 (1), 5804 (2023)
   PUBMED   37726325
  REMARK    GeneRIF: Prosaposin maintains lipid homeostasis in dopamine neurons
            and counteracts experimental parkinsonism in rodents.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2676)
  AUTHORS   Bhattacharya,P., Dhawan,U.K., Hussain,M.T., Singh,P., Bhagat,K.K.,
            Singhal,A., Austin-Williams,S., Sengupta,S. and Subramanian,M.
  TITLE     Efferocytes release extracellular vesicles to resolve inflammation
            and tissue injury via prosaposin-GPR37 signaling
  JOURNAL   Cell Rep 42 (7), 112808 (2023)
   PUBMED   37436891
  REMARK    GeneRIF: Efferocytes release extracellular vesicles to resolve
            inflammation and tissue injury via prosaposin-GPR37 signaling.
REFERENCE   4  (bases 1 to 2676)
  AUTHORS   Kitamura,K., Homma,T., Sohel,M.S.H., Fuyuki,A., Miyawaki,S.,
            Onouchi,S. and Saito,S.
  TITLE     Expression patterns of prosaposin and its receptors, G
            protein-coupled receptor (GPR) 37 and GPR37L1, in the mouse
            olfactory organ
  JOURNAL   Tissue Cell 82, 102093 (2023)
   PUBMED   37075680
  REMARK    GeneRIF: Expression patterns of prosaposin and its receptors, G
            protein-coupled receptor (GPR) 37 and GPR37L1, in the mouse
            olfactory organ.
REFERENCE   5  (bases 1 to 2676)
  AUTHORS   Gehrlein,A., Udayar,V., Anastasi,N., Morella,M.L., Ruf,I.,
            Brugger,D., von der Mark,S., Thoma,R., Rufer,A., Heer,D.,
            Pfahler,N., Jochner,A., Niewoehner,J., Wolf,L., Fueth,M.,
            Ebeling,M., Villasenor,R., Zhu,Y., Deen,M.C., Shan,X., Ehsaei,Z.,
            Taylor,V., Sidransky,E., Vocadlo,D.J., Freskgard,P.O. and
            Jagasia,R.
  TITLE     Targeting neuronal lysosomal dysfunction caused by
            beta-glucocerebrosidase deficiency with an enzyme-based brain
            shuttle construct
  JOURNAL   Nat Commun 14 (1), 2057 (2023)
   PUBMED   37045813
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2676)
  AUTHORS   Sun,Y., Witte,D.P. and Grabowski,G.A.
  TITLE     Developmental and tissue-specific expression of prosaposin mRNA in
            murine tissues
  JOURNAL   Am J Pathol 145 (6), 1390-1398 (1994)
   PUBMED   7992842
REFERENCE   7  (bases 1 to 2676)
  AUTHORS   Kozak,C.A., Adamson,M.C. and Horowitz,M.
  TITLE     Genetic mapping of the mouse prosaposin gene (Psap) to mouse
            chromosome 10
  JOURNAL   Genomics 23 (2), 508-510 (1994)
   PUBMED   7835907
REFERENCE   8  (bases 1 to 2676)
  AUTHORS   Sprecher-Levy,H., Orr-Urtreger,A., Lonai,P. and Horowitz,M.
  TITLE     Murine prosaposin: expression in the reproductive system of a gene
            implicated in human genetic disease
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 40 (2), 233 (1994)
   PUBMED   8003952
  REMARK    Correction to:[Cell Mol Biol (Noisy-le-grand). 1993
            May;39(3):287-99. PMID: 8334382]
REFERENCE   9  (bases 1 to 2676)
  AUTHORS   Sprecher-Levy,H., Orr-Urtreger,A., Lonai,P. and Horowitz,M.
  TITLE     Murine prosaposin: expression in the reproductive system of a gene
            implicated in human genetic diseases
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 39 (3), 287-299 (1993)
   PUBMED   8334382
  REMARK    Erratum:[Cell Mol Biol (Noisy-le-grand). 1994 Mar;40(2):233. PMID:
            8003952]
REFERENCE   10 (bases 1 to 2676)
  AUTHORS   Tsuda,M., Sakiyama,T., Endo,H. and Kitagawa,T.
  TITLE     The primary structure of mouse saposin
  JOURNAL   Biochem Biophys Res Commun 184 (3), 1266-1272 (1992)
   PUBMED   1590788
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY325400.1, AK148545.1 and
            S36200.1.
            
            [WARNING] On Mar 22, 2024 this sequence was replaced by
            NM_011179.4.
            
            On Mar 28, 2009 this sequence version replaced NM_011179.2.
            
            Summary: This gene encodes a multifunctional glycoprotein that
            plays a role in the intracellular metabolism of various
            sphingolipids or secreted into the plasma, milk or cerebrospinal
            fluid. The encoded protein undergoes proteolytic processing to
            generate four different polypeptides known as saposin A, B, C or D,
            that are required for the hydrolysis of certain sphingolipids by
            lysosomal hydrolases. Alternately, the encoded protein is secreted
            into body fluids where it exhibits neurotrophic and myelinotrophic
            activities. A complete lack of the encoded protein is fatal to mice
            either at the neonatal stage or within the first month due to
            severe leukodystrophy and sphingolipid accumulation. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms that may undergo similar processing to generate the mature
            saposins. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longest isoform (B).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.223430.1,
                                           SRR1660815.110933.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                BY325400.1         1-85
            86-2655             AK148545.1         2-2571
            2656-2676           S36200.1           2547-2567
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 30.02 cM"
     gene            1..2676
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /note="prosaposin"
                     /db_xref="GeneID:19156"
                     /db_xref="MGI:MGI:97783"
     exon            1..155
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1789
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /note="isoform B precursor is encoded by transcript
                     variant 2; sulfated glycoprotein 1; snoRNA MBII-198"
                     /codon_start=1
                     /product="prosaposin isoform B precursor"
                     /protein_id="NP_035309.3"
                     /db_xref="CCDS:CCDS35911.1"
                     /db_xref="GeneID:19156"
                     /db_xref="MGI:MGI:97783"
                     /translation="MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGA
                     VKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSL
                     SASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPE
                     VDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFI
                     QGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEV
                     KRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNN
                     ATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAA
                     RPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVL
                     YLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFV
                     CSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN"
     exon            156..289
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            290..364
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            365..490
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            491..688
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            689..832
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            833..889
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            890..898
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1030
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1126
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1406
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1564
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1645
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1753
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..2660
                     /gene="Psap"
                     /gene_synonym="SGP-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2637..2642
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Psap"
                     /gene_synonym="SGP-1"
     polyA_site      2660
                     /gene="Psap"
                     /gene_synonym="SGP-1"
ORIGIN      
        1 gtttgggcgg ggcccgcgcc tgcgcactgg cgtctgcggc gaggttgggg ggtagattta
       61 tattcgtctg gtcagctgac gttctgcatt gcagcctgcg aagtgaagcg gcgccatgta
      121 cgccctcgcc ctcttcgcca gccttctggc caccgctctg accagccctg tccaagaccc
      181 gaagacatgc tctgggggct cagcagtgct gtgcagagat gtgaagacgg cggtggactg
      241 tggggccgtg aagcactgcc agcagatggt ctggagcaag cccacagcga aatcccttcc
      301 ttgcgacata tgcaaaactg ttgtcaccga agctgggaac ttgctgaaag ataatgctac
      361 gcaggaggag atccttcatt acctggagaa gacctgtgag tggattcatg actccagcct
      421 gtcggcctcg tgcaaggagg tggttgactc ttacctgcct gtcatcctgg acatgattaa
      481 gggcgagatg agcaaccctg gggaagtgtg ctctgcgctc aacctctgcc agtcccttca
      541 ggagtacttg gccgagcaaa accagaaaca gcttgagtcc aacaagatcc cggaggtgga
      601 catggcccgt gtggttgccc ccttcatgtc caacatccct ctcctgctgt accctcagga
      661 tcacccccgc agccagcccc aacctaaggc taacgaggac gtctgccagg actgtatgaa
      721 gctggtgtct gatgtccaga ctgctgtgaa gaccaactcc agctttatcc agggcttcgt
      781 ggaccacgtg aaggaggatt gtgaccgctt ggggccaggc gtgtctgaca tatgcaagaa
      841 ctacgtggac cagtattccg aggtctgtgt ccagatgttg atgcacatgc aggatcagca
      901 acccaaggaa atctgtgtgc tggctggctt ctgtaatgag gtcaagagag tgccaatgaa
      961 gactctggtc cctgccaccg agaccattaa gaacatcctc cctgccctgg agatgatgga
     1021 cccctatgag cagaatctgg tccaggccca caatgtgatt ttatgccaga cctgtcagtt
     1081 tgtgatgaat aagttttctg agctgattgt caataatgcc actgaggagc tcctagttaa
     1141 aggtttgagc aacgcatgcg cactgctccc cgatcctgcc agaaccaagt gccaggaggt
     1201 ggtgggaaca tttggcccct ccctgttgga catctttatc catgaggtaa accccagctc
     1261 tctgtgcggt gtgatcggcc tctgtgctgc ccgcccggag ttggtggagg cacttgagca
     1321 gcctgcgcca gccattgtat ctgcactgct caaagagccc acaccgccaa agcagcccgc
     1381 acagcccaag cagtcggcat tgcccgccca tgtgcctcct cagaagaatg gtgggttctg
     1441 tgaggtgtgc aagaaactgg tcctctattt ggaacataac ctggagaaaa acagcaccaa
     1501 ggaggaaatc ctggccgcac ttgagaaggg ctgcagcttc ctgccagacc cttaccagaa
     1561 gcagtgcgat gactttgtgg ctgagtatga gcccttgcta ttggagatcc tcgtggaagt
     1621 gatggatcct ggatttgtgt gctcgaaaat tggagtttgc ccttctgcct ataagctgct
     1681 gctgggaacc gagaagtgtg tctggggccc tagctactgg tgtcagaaca tggagactgc
     1741 cgcccgatgc aatgctgtcg atcattgcaa acgccatgtg tggaactagt ttcccagctg
     1801 cagaagtcac ctacttgtgg gtctagggta atgaacacat agatctattt gacttaataa
     1861 gtaggaaccc cctttgccct tcccccatct cctctccctt actgtagcat ttctgtcatg
     1921 taagaggtgc tgacagccac ttccgtgtcc cctttctgct cgaaggatga ggataccttg
     1981 ggcatcagct ccccggctgc ccttttcacc cacctgctgg aggggggtgg tgagccagag
     2041 ggcaggagca ttttctgagc cctttcttgg tgtgtggggg atctatggcc atctcctacc
     2101 atgagggagc tacccagctt cctgtggtac caaggagtta ttttggatga ttagaagcac
     2161 agaatgatca ggcctttaga gcgatggaat ggccattgtc atagcacaga gatttcagaa
     2221 gcacctgcag gtggcttgct tgggatgttg ctgtccctgg gtcagccttc cattctgctt
     2281 tcctgtcttc ccgtctgcct tgttggggtt ctgtggggta gggtggggag gggaaacttg
     2341 tgaatgtaac ttgcctgtgc cgtgtgacgg tcacgtgggc ctggtctttt gtgtgtgagg
     2401 cccttgaccg tgtggcctct gcctggctgt ttggggtcct gcacggcttt cccaccacct
     2461 gtagctcttg ttgacctgcc tgttcacctc atgagtgaag cgtctgcctg gcagtgggcc
     2521 atgaactgag gggtctctgt gtagagtaga agcttcctgt gcctccggtt gccaggagac
     2581 agcctgtgca gttaaatgga cctagatttt gttttgcact aaagtttctg tgacttaata
     2641 aagttctgtt aaccaacaga aaaaaaaaaa aaaaaa
//
