LOCUS       NM_181348              12512 bp    mRNA    linear   ROD 02-JUL-2024
DEFINITION  Mus musculus prune homolog 2 (Prune2), mRNA.
ACCESSION   NM_181348 XR_032740 XR_035391
VERSION     NM_181348.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12512)
  AUTHORS   Islam,S., Ueda,M., Nishida,E., Wang,M.X., Osawa,M., Lee,D.,
            Itoh,M., Nakagawa,K., Tana and Nakagawa,T.
  TITLE     Odor preference and olfactory memory are impaired in
            Olfaxin-deficient mice
  JOURNAL   Brain Res 1688, 81-90 (2018)
   PUBMED   29571668
  REMARK    GeneRIF: Study shows that odor preference and olfactory memory are
            impaired in Prune 2deficient mice. Knockout of Prune2 leads to a
            decrease in the Prune2 protein isoform Olfaxin. Levels of Olfaxin
            mRNA were decreased.
REFERENCE   4  (bases 1 to 12512)
  AUTHORS   Boulay,A.C., Burbassi,S., Lorenzo,H.K., Loew,D., Ezan,P., Giaume,C.
            and Cohen-Salmon,M.
  TITLE     Bmcc1s interacts with the phosphate-activated glutaminase in the
            brain
  JOURNAL   Biochimie 95 (4), 799-807 (2013)
   PUBMED   23246912
  REMARK    GeneRIF: kidney-type glutaminase (KGA) as a novel partner of Bmcc1s
REFERENCE   5  (bases 1 to 12512)
  AUTHORS   Li,S., Hayakawa-Yano,Y., Itoh,M., Ueda,M., Ohta,K., Suzuki,Y.,
            Mizuno,A., Ohta,E., Hida,Y., Wang,M.X. and Nakagawa,T.
  TITLE     Olfaxin as a novel Prune2 isoform predominantly expressed in
            olfactory system
  JOURNAL   Brain Res 1488, 1-13 (2012)
   PUBMED   23059019
  REMARK    GeneRIF: We showed that Olfaxin, a novel Prune2 isoform contains a
            BCH motif and localizes predominantly to the synaptic cytosol in
            the olfactory bulb and layer Ia of the piriform cortex.
REFERENCE   6  (bases 1 to 12512)
  AUTHORS   Li,S., Itoh,M., Ohta,K., Ueda,M., Mizuno,A., Ohta,E., Hida,Y.,
            Wang,M.X., Takeuchi,K. and Nakagawa,T.
  TITLE     The expression and localization of Prune2 mRNA in the central
            nervous system
  JOURNAL   Neurosci Lett 503 (3), 208-214 (2011)
   PUBMED   21893162
  REMARK    GeneRIF: Prune2 mRNA is predominantly expressed in the neurons of
            the cranial nerve motor nuclei and the motor neurons of the spinal
            cord
REFERENCE   7  (bases 1 to 12512)
  AUTHORS   Cheng,T.W. and Gong,Q.
  TITLE     Secreted TARSH regulates olfactory mitral cell dendritic complexity
  JOURNAL   Eur J Neurosci 29 (6), 1083-1095 (2009)
   PUBMED   19302145
REFERENCE   8  (bases 1 to 12512)
  AUTHORS   Visel,A., Carson,J., Oldekamp,J., Warnecke,M., Jakubcakova,V.,
            Zhou,X., Shaw,C.A., Alvarez-Bolado,G. and Eichele,G.
  TITLE     Regulatory pathway analysis by high-throughput in situ
            hybridization
  JOURNAL   PLoS Genet 3 (10), 1867-1883 (2007)
   PUBMED   17953485
REFERENCE   9  (bases 1 to 12512)
  AUTHORS   Machida,T., Fujita,T., Ooo,M.L., Ohira,M., Isogai,E., Mihara,M.,
            Hirato,J., Tomotsune,D., Hirata,T., Fujimori,M., Adachi,W. and
            Nakagawara,A.
  TITLE     Increased expression of proapoptotic BMCC1, a novel gene with the
            BNIP2 and Cdc42GAP homology (BCH) domain, is associated with
            favorable prognosis in human neuroblastomas
  JOURNAL   Oncogene 25 (13), 1931-1942 (2006)
   PUBMED   16288218
REFERENCE   10 (bases 1 to 12512)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK039241.1, AC126674.3, BC094224.1 and AC147026.2.
            
            On or before Jul 1, 2009 this sequence version replaced
            XR_035391.1, XR_032740.1, NM_181348.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849390,
                              SAMN01164133 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2540              AK039241.1         2-2541
            2541-5756           AC126674.3         34661-37876         c
            5757-10244          BC094224.1         1-4488
            10245-12512         AC147026.2         87302-89569         c
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 12.34 cM"
     gene            1..12512
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /note="prune homolog 2"
                     /db_xref="GeneID:353211"
                     /db_xref="MGI:MGI:1925004"
     exon            1..175
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..9394
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /note="neuronal protein; BNIP2 motif-containing molecule
                     at the C-terminal region 1"
                     /codon_start=1
                     /product="protein prune homolog 2"
                     /protein_id="NP_851993.3"
                     /db_xref="CCDS:CCDS50400.1"
                     /db_xref="GeneID:353211"
                     /db_xref="MGI:MGI:1925004"
                     /translation="MEEFLQRAKSKLDRSKQLEQVHAVIGPKSCDLDSLISAFTYAYF
                     LDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNIPESFHIFRDEINLHQLNDEGK
                     LSITLVGSHVLGSEDRTLESAVVRVINPGEQSDGELGFPETSSSLVLKELLREAPELI
                     TQQLAHLLRGSILFTWMSMDPELPEKQEEILSILEEQFPNLPPRDDIINVLQESQLSA
                     QGLSLEQTMLKDLKELSDGEIKVAISTVNMTLEDYLLHGNITSDLKAFTDKFGFDVLI
                     LISSFTWEEQQRQQIAVYSQNLELCSQICCELEESQNPCLELEPFECGCDEILVYQQE
                     DPSVTSDQVFLLLKEVINRRCAEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIE
                     PQPSSVNFIENPPELNDSNQAQADGNIDLVSPDSGLATIRSSRSSKESSVFLSDDSPV
                     GDGGAPHHSLLPGFDSYSPIPEGIVAEEHAHSGEHSEHFDLFNFDSAPIASEQSQPSS
                     HSADYSPEDDFPNSDSSEGNLSAGPKGLGEMGINMSNYSSSSLLSEAGKDSLVEFDEE
                     FIQRQESSGDNSERNLSLTCFAGEEPSSPERLKNPGKMIPPTPMNSFVEISPSNEEPT
                     PLYPEDIIQNAIDTGHLGPPQARARCRSWWGGLEIDSKNVVDTWNASEQESVFQSPEP
                     WKDPKPEPVERRTSDSTFQPKSLEFSKSDPWESEFGQPELGNKEAQDQKEESLQYQHL
                     PTVRPHLTDASPHGTNHLIEDFAALWHSGHSPTTMPEPWGNPTDAGEAAVTMSFPTWG
                     AFDKEEDNADTLKNTWNLHPTNNETPSGQEPSEWAMGQSGFSFPAADLLDNPLIEVNK
                     DAAPEIWGKNNSSKDTSLTSGSPTSDLGQTWNNSKLPGEDQNGLVDPKATGKVYEKEG
                     SWSLFEESAKKRGADVLAPWEDSFLSYKCSDYSASNIGEDSVPSPLDTNYSTSDSYTS
                     PTYAGDEKEIANKPVDKDNGFEAKDAEFPAEGLEVLATSSQQSQRNRIGSGPGNLDMW
                     ALPHTEDKPEGNDAHHPDSDALKTEHAEDKNASMEDDVRESSPSSYDDPSMMRLYEAN
                     RQLTLLHSNTNSRQAAPDSLDTWNRVTLEDTQSTATISDMDNDLDWDDCSGGVAISGD
                     GQAEGYIAANSEPETRFSVKQLEPWGTEHQEANQVDWDLSASAEPTGDPGPSEYQTLN
                     EKTGQLIANSIWDSVMGDKNMPSFRLPSPPNTVDMEHGTWPSESPRHSNGKDSHMLEA
                     SRLSESGGLTSQPVNQDTWGDSQGDTASSVTGLASPEHFAQSDPWTGHTYGQSESEIE
                     GLVASDCEHLDKEAALGSGVNGAPWAFGKKPRDQEFSSSDAFEHQDISSASGKISSLS
                     VTSSPQSEEPGEALEVEKEPFILDSLAVQTETFTWDLQSKDTHEESLVDHRNLGEANT
                     TLDRINPMKNRPLSGMELEKTEACTILKPERANGKLLYESSQDFGVWDGPMDSDVWDS
                     HISYETAMNSTGQRTEERSLEALSPGNYDRDSLSSGCTHSSASSPDLHDSSVALSSWT
                     YGPSAEHQKENHDDANKQIHQESELFTTEAHVDVITEMKDFVENREDGFGKMSNQEDP
                     QFPEIPNDPFNSGPSSSSSSLGADKYSEYSHAYQEGDLTTKRQEENELGFLEIVEPEG
                     TRIISTSSGSGNDSGGDEELLEKELHLATVAQSEAGACTSLHEPAFSATEHSKPEFSV
                     FVGSLESIEKENKSSPFSDSQQSSPGQWILSPLMQADTQDTCKEETRAAETGTMDTTW
                     HGSASTEAKNGDPDKLEMLGFSADSTEWWNAGAQEGRANAGMSAEELSNSEGELEPTS
                     PVFQNAGPWSLPIQNDSEPVDTGSTNPFRGKLKSPVLDSHGDKSQEKLWNIQPKQLDS
                     DANQLSQLVILDQMKDKDSGQQTAMSPAAGDLPAETLTQGQGRESMLSVWDRAEPALT
                     HRDENGCVSTGVSPTECQQENQWEPEKPYLSHVTHSSTPTENALESNAPTQLMRKLDS
                     DWNSPSPSEPQHNFVPDILHGNFEEGGQLASASPDLWMDVKQPFSFKVDSENPDILTH
                     CDHDSNSQASSSPDVCHDSEGEQKMEKHTAVYLGLEVEPSEFSLTEPNMNDEPTWEPE
                     QESLPHNSELHSEHAMPLPPIDSQNDINNSSKPASSRSSPEPSDMRGDNNTSVTSMEE
                     DTNPEVEAVDSVTIPGHFPRSEDADTFEAHQEVSVEVDDSWVSKDLCPESQTGTRALL
                     DCEQPFASESPAVLTDIFLTSDTCLDVSEAALDHSFSDASGLNTSTGTIDDMSKLTLS
                     EGHPETPVDGDAGKQDVCSSEASWGDFEYDAMGQNIDEELMREPEHFLYGGDLPSEES
                     ALKQSLTPYTPPFDLSYLTEPTGCTETAQGAESPGDESLGSDAAEMLLSALPDHREED
                     KAETNIRKPRYQMTVLHIHEDPEALSSPVGGTGSNNESSPSNIDWEIETDNSDSPAGG
                     DMKPPNGKEILELEEDEKVIPTKGPKQTELEYKEEKQPEQSEDHQVLAVDYILVSHEK
                     DSPLKPEAREARENIPELEQLSIGSRETGLPETQLSGTPDTCQSEFLNDVKVHSAERM
                     SSSSNHESASLENPAQDQSWMVLSHSEVGDPPTETRDSGPESPGRTPEPFLSLSLDKG
                     PKSQVLERNKPLNSLALEEVAGLSSQSRNIERQGQAGLDAVPTQAATHDNEWEMLSPQ
                     LSRKNRNPPQEMEEETQCPEPGPRKPRLKGPPSEDEGMDIHFEEGVLSPSAADMRPEP
                     PNSLDLNGSHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEAD
                     SFEYTNHEDPTANKSSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPY
                     RRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMI
                     VYLNGATPRRKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISS
                     KFSSKIKYVTSLSELSGLIPMDCIHIPESIIKYDEEKSFKRSVRLDEELREASEAAKT
                     SCLYNDPEMSSMEKDIDMKLKEKP"
     exon            176..280
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            281..483
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            484..647
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            648..797
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            798..892
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1048
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..7598
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            7599..8364
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            8365..8444
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            8445..8645
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            8646..8819
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            8820..8922
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            8923..9054
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            9055..9141
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            9142..9240
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            9241..9276
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            9277..9315
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            9316..9363
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
     exon            9364..12512
                     /gene="Prune2"
                     /gene_synonym="6330414G02Rik; A230083H22Rik;
                     A330102H22Rik; Bmcc1; mKIAA0367; Olfaxin"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcccggcc ggcccgccta gggagcggct ggtccctgcg caaagcgacc actttctgga
       61 gatcccgctc aagtccaccg cagctctcct ctctcggcgc tctccatccg gacccctggt
      121 cccagttcct agcagagaca tggaagaatt tttgcaacgc gccaaatcta aactggatcg
      181 aagtaaacaa ttggaacagg tccacgcagt tattggacct aagtcatgtg acctggattc
      241 tctcatttct gccttcacat atgcatactt tctggacaag gtcagtcctc ccggggtcct
      301 ctgcttgcca gtgctgaata tcccaagaac tgaattcaac tacttcacag agacaaggtt
      361 tattttagaa gagctgaaca tccctgaatc attccacata ttccgagatg agattaacct
      421 gcatcagctg aacgatgaag ggaaactgtc gataacgctc gtgggcagcc acgtcctggg
      481 gagtgaggac agaactttgg aatcggcagt tgtcagagtc attaaccccg gagagcagag
      541 tgatggtgag ctggggttcc cggagacttc ctcctctctg gtgctgaagg agctcctgcg
      601 tgaggctcca gaactcatca cccagcagct ggcacatctc ctcagaggca gcatcctgtt
      661 cacgtggatg agtatggatc ctgagctccc ggagaagcag gaggaaatcc tctccatcct
      721 ggaagaacag tttcccaacc tccctccgag ggatgatatc atcaacgtgc tgcaagagag
      781 tcagctcagt gctcagggtt taagtcttga acaaacaatg ctgaaagatc taaaggagct
      841 gtcagacggt gaaataaaag tggccatcag caccgtgaac atgaccctgg aggactacct
      901 gcttcacggc aacatcacca gtgatttaaa agccttcaca gacaagttcg gctttgacgt
      961 cctcattctg atctcaagct tcacatggga ggaacagcag aggcagcaga tcgccgtgta
     1021 ctcccagaac ctggaactgt gcagccagat ttgctgtgag ctagaagaaa gtcagaaccc
     1081 ttgcctggaa ctggagccct tcgaatgtgg ctgtgatgag atcctggtat accagcagga
     1141 agacccttct gtgacctccg accaggtgtt tcttcttctt aaggaagtca tcaataggag
     1201 gtgtgcagag atggtctcta acagtcggac atcctcaaca gaagccgtgg caggcagcgc
     1261 cccgctgtcc cagggctctt ccgggattat ggaattgtat ggatctgaca tagagccaca
     1321 gcccagctct gtgaatttca tagaaaaccc cccagagctc aacgattcta accaagctca
     1381 ggcggatggc aacatagacc tggttagccc tgacagtggg ttggccacca tcagaagcag
     1441 tcgctcgtcc aaggaaagct ccgtcttcct cagtgacgac agccccgtgg gagacggtgg
     1501 tgcgcctcac catagccttc tcccaggatt tgactcctac agccccattc ctgaagggat
     1561 agttgcggag gagcatgctc actccgggga acacagtgaa cactttgacc tcttcaactt
     1621 tgattcagcg cccatagctt cagagcagtc ccagccgtct tcccactctg cagactactc
     1681 gccagaagac gacttcccca acagcgattc atcagaaggg aacctctctg ccgggcctaa
     1741 ggggcttggt gagatgggga tcaacatgtc caattactcc tctagttctc ttttgtcaga
     1801 ggctggtaaa gacagccttg tggaatttga tgaagagttt atccagcgac aagaaagctc
     1861 aggggataac tctgaaagaa atttaagcct gacatgtttt gcgggagagg agccttcttc
     1921 ccctgaaagg ctgaagaatc ctggaaagat gatcccacca acacctatga acagctttgt
     1981 ggaaatctca ccatcaaatg aggagccaac tccactctat cctgaagata taatccaaaa
     2041 tgcaattgac accggacatc tgggcccacc tcaggcccgt gcacggtgca gaagctggtg
     2101 gggtggcttg gagattgact ctaaaaacgt tgtagataca tggaacgcca gtgaacaaga
     2161 atctgtcttc cagagccctg aaccatggaa agatcctaag cctgagccag ttgagaggag
     2221 aacctcagat tccacattcc aaccaaagag tcttgaattc tcaaagtcag atccttggga
     2281 atctgagttt ggacagcctg aactgggcaa caaagaagcc caggaccaga aggaggaaag
     2341 cttgcaatac cagcacttgc ctacagtaag gccacatttg acagatgcct ctcctcatgg
     2401 gacaaaccac ttgatagagg actttgctgc tttgtggcat tcaggacact caccaacaac
     2461 aatgcctgag ccctggggaa accccaccga tgctggtgaa gctgcagtta cgatgtcctt
     2521 ccccacctgg ggtgcatttg ataaagaaga agataatgct gacacattaa agaatacttg
     2581 gaatctccat cccactaaca acgagacacc ttcagggcag gaaccaagtg aatgggctat
     2641 gggccaaagt gggttttcat tccctgcagc agacctactt gacaatcccc tcattgaggt
     2701 aaataaagat gcagctccag aaatctgggg caagaacaac agctccaagg ataccagtct
     2761 tacgtctgga agtcccactt cagatctggg tcaaacgtgg aataattcta agctaccagg
     2821 ggaagaccag aatggcttgg tggatcctaa agctacaggg aaggtatatg agaaggaagg
     2881 gtcctggagc ctctttgagg agagtgctaa gaaaagaggg gctgatgttt tagctccttg
     2941 ggaagattcc ttcttgtcct ataaatgttc tgattacagt gcatccaaca taggagaaga
     3001 ttcagtccca tctccattag acaccaacta ctccacctct gactcttaca catcaccaac
     3061 atatgctggg gatgaaaaag aaatagcaaa caagccagtt gacaaagata atggttttga
     3121 ggcaaaagat gctgaatttc ctgcagaggg gcttgaggtt cttgcaacat catcacagca
     3181 gtctcagaga aatcgaattg gttctggtcc tgggaatcta gacatgtggg cattgcctca
     3241 cacagaagat aagcctgaag gaaatgacgc acatcaccca gatagcgatg cgctcaagac
     3301 ggagcacgca gaagacaaaa atgcttccat ggaggatgac gtgagggaaa gcagcccgtc
     3361 cagttacgat gaccctagca tgatgcgact gtacgaggca aaccggcagc tcacccttct
     3421 acacagcaac accaactcac gccaggcagc ccctgacagc cttgacacat ggaaccgagt
     3481 gactctggag gacacgcagt ccactgcaac gatctcggac atggataatg acttggattg
     3541 ggatgactgc agtgggggcg tggcgatcag tggggatggt caagcagaag gttacatagc
     3601 tgcaaacagt gaacctgaaa cccggttctc agtgaagcag ctggaaccat ggggtacaga
     3661 acatcaggaa gcaaatcagg tagactggga tctctctgcc tctgctgagc ccacagggga
     3721 tcctggtccc agtgaatacc agaccctaaa tgagaaaact gggcagttaa ttgcaaacag
     3781 tatttgggat tctgtcatgg gagataaaaa tatgccatca ttcagattac ctagcccacc
     3841 aaatactgta gatatggaac acggcacttg gccttctgaa agtcccaggc actcaaatgg
     3901 taaggacagc cacatgttag aagcctctag actcagtgag tcgggagggc ttacgtctca
     3961 acctgtcaac caggacacgt ggggagactc ccaaggtgac acagcatcct cggtgaccgg
     4021 actggcaagt ccagaacact ttgcacagtc agatccatgg acaggccata cttatgggca
     4081 gagtgaaagt gagattgaag gcctggtggc ctctgattgt gagcatcttg acaaggaggc
     4141 agcgctaggc tctggagtga atggtgcccc gtgggctttt ggaaaaaagc ccagggacca
     4201 ggaattctct tcttcagatg catttgaaca ccaagacatc tccagtgcat caggcaaaat
     4261 cagctctctt tcagtcacct ccagtcctca gagtgaggaa ccaggggagg ccttagaagt
     4321 cgagaaggag cctttcattc tagactccct tgctgtgcaa acagagacat tcacatggga
     4381 tttgcaaagc aaagacaccc atgaagaatc tctagtggat catagaaatt taggtgaagc
     4441 caacacaaca ttggatagga ttaatccaat gaaaaacagg cccttatctg gaatggaact
     4501 tgagaaaact gaagcctgta ccattctgaa gccagaaaga gcaaatggaa aactacttta
     4561 tgaatcttct caagactttg gtgtttggga tggcccgatg gatagtgatg tgtgggatag
     4621 tcatataagt tatgagacag ctatgaattc tacaggtcag agaactgagg aaagatctct
     4681 agaagccctt tcaccaggaa actatgacag agacagcctc tctagtgggt gtacgcattc
     4741 cagtgcatca agtcctgacc tacatgattc ctcagtagcc ttgtcctcct ggacttacgg
     4801 acccagtgct gaacatcaga aagagaatca tgatgatgcg aacaaacaga ttcaccaaga
     4861 atcagaacta tttaccaccg aagcccatgt agatgtcatt actgaaatga aggactttgt
     4921 agaaaacagg gaggatggct ttggaaagat gtccaatcag gaagatcctc aattcccaga
     4981 gattccaaat gatcccttca acagtggtcc ctcatcctct tcatccagcc ttggagcaga
     5041 taaatattca gaatattctc atgcatatca ggaaggggat ctaacgacta aacgtcaaga
     5101 ggaaaatgaa cttggttttc tggaaattgt ggagccagag ggtaccagga tcatatccac
     5161 aagctcaggt tctggcaatg acagtggggg tgacgaagag ttgctagaga aggagctcca
     5221 tttggccaca gttgctcaga gtgaagctgg ggcttgcact tcattgcatg aacctgcgtt
     5281 ctcggccaca gagcattcca agcctgagtt ttctgtcttt gtgggtagtt tggagtcaat
     5341 agagaaagag aacaagtcaa gcccgttcag tgacagtcaa cagagcagcc ctggtcagtg
     5401 gattttgtca ccactcatgc aggctgacac acaggacaca tgtaaggagg agaccagagc
     5461 tgcagaaacg gggacaatgg ataccacgtg gcatggatct gccagcacgg aagctaagaa
     5521 tggggaccct gacaaattgg aaatgcttgg cttctcagct gacagcactg agtggtggaa
     5581 cgctggggca caggaaggga gagcaaacgc cggtatgtct gcagaggagt tgtctaactc
     5641 agagggtgag ctagagccta cttctccagt gttccagaac gctggtccgt ggagcttacc
     5701 cattcagaat gatagtgaac ctgtggacac aggcagcacc aatcctttcc gtggtaaact
     5761 aaagtcaccc gtcctagata gtcatggaga caagtcacag gagaaacttt ggaacattca
     5821 acctaagcag ctggattcag atgccaatca actcagccag cttgtgatcc tggaccaaat
     5881 gaaggacaaa gattctggac agcaaacagc catgtctcct gctgctggtg atcttcctgc
     5941 agaaactctc acccaagggc agggccggga atctatgctg tctgtctggg accgtgcaga
     6001 accagcatta actcacagag atgaaaatgg atgtgttagc actggggttt cacccactga
     6061 gtgccaacaa gagaaccagt gggaaccaga aaaaccctat ctcagccacg tgacacattc
     6121 aagcactccc acagaaaatg ccctcgagag caatgccccc acacaactga tgaggaagct
     6181 ggactctgat tggaatagcc ccagccccag cgaaccccaa cataactttg ttccagatat
     6241 tttacatggc aacttcgaag agggtgggca gctggcctcg gcgtcacctg atttgtggat
     6301 ggatgtcaag caacccttca gttttaaagt agatagcgag aatcctgata tattgactca
     6361 ctgtgaccat gacagcaact ctcaggcatc cagcagccct gacgtgtgcc acgattctga
     6421 aggggagcaa aagatggaga agcatactgc tgtttacctg ggacttgaag tggaacccag
     6481 tgagttttct ctcaccgagc caaacatgaa tgatgaacca acttgggaac ctgagcagga
     6541 aagtctccca cacaattcag aactccattc cgaacacgca atgcccttgc ctccaatcga
     6601 cagtcagaat gatataaata actcatctaa gcctgcctct tcaagaagtt ccccagaacc
     6661 ctctgatatg cgcggagaca acaacacaag tgtaacatcg atggaagaag acaccaaccc
     6721 agaggtagaa gcagtggaca gcgtgacgat cccaggtcac ttccccagaa gtgaagacgc
     6781 tgacactttt gaagctcacc aagaggtcag tgtggaagtc gacgactctt gggtgtcaaa
     6841 agacctttgt cctgaaagtc agacaggcac tagagctctg cttgactgtg agcaaccgtt
     6901 tgcttccgag agccctgctg tacttacgga catcttccta acctcagaca cctgcctgga
     6961 tgtaagcgaa gctgccttgg atcacagttt cagtgatgcc tccggcctca acacttccac
     7021 aggcactata gacgacatga gtaagctgac tctatcggaa ggccatcccg aaacaccagt
     7081 tgacggggat gcagggaagc aagatgtctg ttcatctgaa gcttcctggg gtgattttga
     7141 atatgatgca atgggtcaga atattgacga agagctgatg agagagcctg aacacttcct
     7201 ctatggtggt gatcttccgt cagaggaaag cgcgctgaag caatcactga caccatacac
     7261 accccccttt gatttgtcct atctcacgga acctactgga tgcactgaaa ctgcacaggg
     7321 agctgagtct ccaggtgatg aatctctggg aagtgacgcg gcagagatgt tgctttctgc
     7381 tcttccagat cacagggagg aagacaaggc tgagaccaac atcaggaaac ctagatacca
     7441 gatgactgtg ttgcatattc atgaagaccc tgaggctctt tcatcacctg tgggaggaac
     7501 aggttccaat aatgagtcat ctccatcaaa cattgactgg gaaatagaaa cagataattc
     7561 agattcacca gcaggtggcg acatgaaacc accaaacggc aaggagatac tggagttaga
     7621 agaagatgag aaagtaattc ccacgaaagg gccaaagcaa acagaattag aatacaaaga
     7681 agaaaaacag ccagagcaaa gtgaagacca tcaggtacta gctgtggact acatccttgt
     7741 aagccatgaa aaagattcac cattgaaacc agaggccagg gaagcaagag aaaacatacc
     7801 cgaattggaa caattatcca taggttccag agaaacaggg ctaccagaga ctcagttatc
     7861 aggcacccca gacacatgtc agtctgagtt tttaaatgat gtcaaagttc attctgcaga
     7921 gagaatgtct tcgtcttcca accacgagag tgcatctctt gaaaaccctg cacaagacca
     7981 gagttggatg gtcttgagcc atagtgaggt tggtgacccg cctacagaaa ccagggactc
     8041 ggggcctgag tctcctggta gaactccgga gccgttcttg agtctcagtt tggacaaggg
     8101 ccccaagtcc caggttctgg aaagaaataa gcctctaaat tctttagcac tagaggaagt
     8161 tgctggtttg agcagccaat caaggaacat cgagagacaa ggtcaagctg gtctagatgc
     8221 agttccaacc caggcggcca cccacgacaa tgaatgggaa atgctttcgc cacagctctc
     8281 ccggaaaaac aggaaccctc cacaggaaat ggaagaagag acacagtgcc ctgagcctgg
     8341 acccagaaag ccaagactga aaggaccccc gtcagaggat gaaggaatgg atatccactt
     8401 cgaggaggga gtgctgagtc ccagtgccgc agacatgagg cccgaacctc ccaattctct
     8461 ggatctcaat ggcagtcacc ctagaagaat caagctcaca gccccaaata tcaacctctc
     8521 tctggaccaa agtgaaggat ctattctctc tgatgataac ctagacagtc cggatgagat
     8581 tgacatcaat gtggatgaac tcgatacccc tgatgaagca gactcttttg aatatactaa
     8641 ccatgaagat cccacggcca acaaaagctc tggtcaagag tcagagtcta ttccagaata
     8701 tacggctgaa gaggagcgag aagacaaccg gctgtggagg acggtggtca tcggggagca
     8761 ggagcaacgg attgacatga aggtcattga accctacagg agagtcattt ctcacggagg
     8821 atactatggg gacggtctga atgccatcat tgtgtttgct gcctgctttc tgccagacag
     8881 cagccgggcg gattaccact atgtcatgga aaaccttttc ctatatgtaa taagtacctt
     8941 agagctgatg gttgctgaag actacatgat cgtgtactta aatggtgcaa ccccaaggcg
     9001 gaagatgcca ggactaggtt ggatgaagaa atgctaccag atgattgaca gacggttgcg
     9061 caagaatttg aagtcattca tcattgttca cccatcttgg ttcatcagga caatcttagc
     9121 agtgactcgg ccttttataa gctctaaatt cagcagtaaa attaaatatg tgactagctt
     9181 atctgagctc agtggcctga tccccatgga ctgcatccac attccagaga gcatcatcaa
     9241 gtatgatgaa gagaaatctt ttaagagaag tgtgagactg gatgaagaac tgagggaggc
     9301 atccgaagca gctaaaacta gctgccttta caatgatcca gaaatgtctt ctatggaaaa
     9361 ggacattgac atgaagctga aggagaagcc gtagctggcc tgctggagga ggactccctt
     9421 tctgcttggt gattttgctg ggacgtacct acctggaagc aacagggatg gtgacatctc
     9481 cactttgcac cacctggtgc cattggaaac tttcaaggaa aagagaattt gtttactgtt
     9541 aacatatcct atgaaatgtt gtgtcttttc ctattttgcc aattacgtgc ctcaatgatt
     9601 ttagttgaac cttagcaact aaaccttccg tttagtttta tggaccttcc atttttacta
     9661 ttatgctaat cttcggtgca gtgggacttg gttccttaga ctggttttta ccaaaagcat
     9721 agcctagttc tgctgttaga cagatgtgga tatgtttctg tagactgact gaatccctac
     9781 agagcttcct tctagattca ggatgcacag ggcttgcctt gtcccaggaa catggctttc
     9841 tggaactcta tgaaatgctg aacttgaata ggacagacag acagatataa aatcctcatc
     9901 agctaaggca tctgccccct tcctgtccac atctttcagt ttttcaaaag gacccgggct
     9961 ttctaaccca tgcaagcttt actttatatt tctgcatgta aattgctctt ttacaacatc
    10021 atcttctagt gagctttgaa gaactgcatc ttgtgaaact taagacgagc tatgcttcag
    10081 gccacagaac aggctccaac ctttacactt cgtccaactc caaggtggcc cactgagaaa
    10141 tcactgggta acatcgaatc gtccaaaatt aacactcaga tgcattgcaa aagcaatctg
    10201 cttgaggaag tttaaaatta aatacaagta tatactagcc tttatgaaat agctcatttc
    10261 ttgtttttta tatgccccta aattgttacg gcaatttcca gtctgtggat ctccagcaga
    10321 ggttggcaag ctgtaccgag gaggctaagg ctccaaagca agagaaggtg gcttccacac
    10381 ttacagttga aaacaaaaat taccacttaa ctgtctgaag cttcttttac tcattctcca
    10441 ccagaactga acagtgacaa cagacagttc ttcacaaagc cagaacacta tctttacagg
    10501 agaaaaaaca aagtgtgctg acccctacga gaccaaatat ccattccctc cctggtctcc
    10561 ctgctctgag tatttattta aatgctttcc ccaactgagt acacagaggt ggttgtaatc
    10621 tcatttggtt ttgtcaaaaa catttttatt ttctttcaag gcaggatctc accatgtagc
    10681 ccaaattggc ctagaactca ctatgtagac caagtcagcc tcaaagttgt ggcaatccgc
    10741 ctgccttagc ctcccaagtg caggtattat tataggcttg caccatcata tctggacttg
    10801 taaagaaaat ttcgacattt acagacagtg aaacatcacc tgtggcctac tgagaaaagc
    10861 gatgggtctt tctcacaaaa gtgtggctct cttaaagcat gggattgcta gactcaaact
    10921 gaaaatgtcc gaaaactctt tagactcact cactcttcaa agtcatccgt taccaagcag
    10981 ctggcaagac aggccctgcc tggtggcttt gtaggagagc tgcagacact ctgagccctg
    11041 aagaagtctc ctcaacaggg aggcttctta gcccaggtgc cagaggtctt ctgactttat
    11101 ctgcttcatg tctgtttcca atctgtcttc cgtacaagct cacacaatat acgtgagccg
    11161 gtcggttccc ttcccttcat aaagctccat tatttacttt cacctgttga tcctgttcaa
    11221 cttagttttt tttacctttg tctttttttc aatctccctt ctctctctct ctcttttttt
    11281 tttttttttt tttttttttt tttttttttt gctaattcga tagaaaaacc tcagaatcat
    11341 ctaggctccc acagcctgcc ctgccctttg gaagcccacc tcaaacatca tggtgatagg
    11401 aatatagtta cccaatatcc tgattcgctc acctgcttaa catattaact cctttatatt
    11461 ccattctgga agatgaattt cacatttccc ctagccatgt taaacttatg ttgttgtgag
    11521 aactgcagag tttggctcag cagatctgta gacatgggac tcttttcata accaaccaga
    11581 catggcaaca ggcaaacagg cacaccattc acactttctc tgagtctgtg tatggaaaat
    11641 gactcactcg tgtggcatgg caaagtaaat ttaatgtgag cagtttcacg tcaatgatat
    11701 ttggaaaatc ctatgggctg taataccatt acaatattta agcacatgtt atcttactat
    11761 cttcaaaaaa caagttactt taattgcatg tgctagagaa aagctatcat gctcatcaaa
    11821 tgaataggct tctagggaat tctgggaaaa tagcccataa acaggtagat gtaactctca
    11881 cgtcgctcag ctgtagcgcc tgcaccctgg gcattcttgc tttggtctgg actgccagga
    11941 cctgctttgc actttttcct gtagatttgt agttaaggga acaccgaggg gtcattgagg
    12001 caggagtggt gttcactctg agttccagct agtacttagc tggattttga ctaaggctga
    12061 aacaaaaacc ttcacagaag gtagttgttg aagagtgaga acaactgggg gtggaggaag
    12121 accccaaagt tcctattgaa gccatacagt gttatataag gttcctccct agggcatact
    12181 gatgagcaaa gaggccaccc tccctagcac acatccttag gatgtaactt tgagagtccc
    12241 ttcatggttc tgcacaaccc ttgtcttatc actatgcccc agtggtctgc tactttagga
    12301 atatctaggt gtgcacaaac ttaacctttt ctatatctaa agagttttct acgtgtatcg
    12361 gattgctcag aataaagtat atattagacg agttttggta aagaaataaa ttctatctgg
    12421 atagtatggt ttaaaaatat gaaaatatgt taagaacaca atgtattgct atacccttac
    12481 tgtgtaattc agaaataaaa atagattcca ca
//
