LOCUS       NM_146227               1455 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus serine protease 50 (Prss50), transcript variant 1,
            mRNA.
ACCESSION   NM_146227 XM_917109
VERSION     NM_146227.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Li,J., Niu,C., Ai,H., Li,X., Zhang,L., Lang,Y., Wang,S., Gao,F.,
            Mei,X., Yu,C., Sun,L., Huang,Y., Zheng,L., Wang,G., Sun,Y.,
            Yang,X., Song,Z. and Bao,Y.
  TITLE     TSP50 Attenuates DSS-Induced Colitis by Regulating TGF-beta
            Signaling Mediated Maintenance of Intestinal Mucosal Barrier
            Integrity
  JOURNAL   Adv Sci (Weinh) 11 (11), e2305893 (2024)
   PUBMED   38189580
  REMARK    GeneRIF: TSP50 Attenuates DSS-Induced Colitis by Regulating
            TGF-beta Signaling Mediated Maintenance of Intestinal Mucosal
            Barrier Integrity.
REFERENCE   3  (bases 1 to 1455)
  AUTHORS   Scovell,J.M., Bournat,J.C., Szafran,A.T., Solis,M., Moore,J.,
            Rivera,A., Chen,C.H., Zhang,J., Wilken,N., Seth,A. and Jorgez,C.J.
  TITLE     PRSS50 is a testis protease responsible for proper sperm tail
            formation and function
  JOURNAL   Development 148 (8) (2021)
   PUBMED   33913480
  REMARK    GeneRIF: PRSS50 is a testis protease responsible for proper sperm
            tail formation and function.
REFERENCE   4  (bases 1 to 1455)
  AUTHORS   Ai,H.H., Liu,B., Yang,M.T., Zuo,Q.Q., Song,Z.B., Bao,Y.L.,
            Sun,L.G., Zhou,L. and Li,Y.X.
  TITLE     Expression and effects of TSP50 in mouse embryo and cardiac myocyte
            development
  JOURNAL   Biochem Biophys Res Commun 502 (2), 283-288 (2018)
   PUBMED   29842883
  REMARK    GeneRIF: TSP50 expression at embryonic day 6.5 (E6.5), day 8.5
            (E8.5) and day 10.5 (E10.5) was increasingly enhanced, However, the
            expression of TSP50 decreased gradually in the development and
            differentiation of cardiac myocyte from E12.5 to postnatal (P0).
REFERENCE   5  (bases 1 to 1455)
  AUTHORS   Harten,S.K., Oey,H., Bourke,L.M., Bharti,V., Isbel,L., Daxinger,L.,
            Faou,P., Robertson,N., Matthews,J.M. and Whitelaw,E.
  TITLE     The recently identified modifier of murine metastable epialleles,
            Rearranged L-Myc Fusion, is involved in maintaining epigenetic
            marks at CpG island shores and enhancers
  JOURNAL   BMC Biol 13, 21 (2015)
   PUBMED   25857663
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1455)
  AUTHORS   Zhou,L., Bao,Y.L., Zhang,Y., Wu,Y., Yu,C.L., Huang,Y.X., Sun,Y.,
            Zheng,L.H. and Li,Y.X.
  TITLE     Knockdown of TSP50 inhibits cell proliferation and induces
            apoptosis in P19 cells
  JOURNAL   IUBMB Life 62 (11), 825-832 (2010)
   PUBMED   21086474
  REMARK    GeneRIF: results showed that downregulation of TSP50 expression in
            P19 embryonal carcinoma stem cells not only reduced cell
            proliferation, colony formation, and migration but also induced
            cell apoptosis
REFERENCE   7  (bases 1 to 1455)
  AUTHORS   Huang,Y., Wang,Y., Wang,M., Sun,B., Li,Y., Bao,Y., Tian,K. and
            Xu,H.
  TITLE     Differential methylation of TSP50 and mTSP50 genes in different
            types of human tissues and mouse spermatic cells
  JOURNAL   Biochem Biophys Res Commun 374 (4), 658-661 (2008)
   PUBMED   18662669
  REMARK    GeneRIF: These findings suggested that DNA methylation might
            regulate the TSP50 and mTSP50 gene expressions in different types
            of tissues and spermatic cells.
REFERENCE   8  (bases 1 to 1455)
  AUTHORS   Cho,K.I., Suh,J.G., Lee,J.W., Hong,S.H., Kang,T.C., Oh,Y.S. and
            Ryoo,Z.Y.
  TITLE     The circling mouse (C57BL/6J-cir) has a 40-kilobase genomic
            deletion that includes the transmembrane inner ear (tmie) gene
  JOURNAL   Comp Med 56 (6), 476-481 (2006)
   PUBMED   17219777
REFERENCE   9  (bases 1 to 1455)
  AUTHORS   Xu,H.P., Yuan,L., Shan,J. and Feng,H.
  TITLE     Localization and expression of TSP50 protein in human and rodent
            testes
  JOURNAL   Urology 64 (4), 826-832 (2004)
   PUBMED   15491742
  REMARK    GeneRIF: TSP50 may perform a critical biologic function in
            mammalian spermatogenesis. It may be a new type of threonine
            protease whose linear catalytic structure is similar to many serine
            proteases, except for a Thr-for-Ser residual catalytic site
            substitution.
REFERENCE   10 (bases 1 to 1455)
  AUTHORS   Mitchem,K.L., Hibbard,E., Beyer,L.A., Bosom,K., Dootz,G.A.,
            Dolan,D.F., Johnson,K.R., Raphael,Y. and Kohrman,D.C.
  TITLE     Mutation of the novel gene Tmie results in sensory cell defects in
            the inner ear of spinner, a mouse model of human hearing loss DFNB6
  JOURNAL   Hum Mol Genet 11 (16), 1887-1898 (2002)
   PUBMED   12140191
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139378.4.
            
            On May 1, 2007 this sequence version replaced NM_146227.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045168.1, BC150686.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-493               AC139378.4         173905-174397
            494-656             AC139378.4         177198-177360
            657-940             AC139378.4         178200-178483
            941-1107            AC139378.4         179668-179834
            1108-1455           AC139378.4         180219-180566
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 60.79 cM"
     gene            1..1455
                     /gene="Prss50"
                     /gene_synonym="79H19C; Tsp50"
                     /note="serine protease 50"
                     /db_xref="GeneID:235631"
                     /db_xref="MGI:MGI:2447303"
     exon            1..493
                     /gene="Prss50"
                     /gene_synonym="79H19C; Tsp50"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1344
                     /gene="Prss50"
                     /gene_synonym="79H19C; Tsp50"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; testes-specific protease 50; testis-specific
                     protease-like protein 50; probable threonine protease
                     PRSS50; protease, serine 50"
                     /codon_start=1
                     /product="probable threonine protease PRSS50 isoform 1
                     precursor"
                     /protein_id="NP_666339.2"
                     /db_xref="CCDS:CCDS23577.1"
                     /db_xref="GeneID:235631"
                     /db_xref="MGI:MGI:2447303"
                     /translation="MEPWCGAEVRGQGPQGPRVPGASRSRSRALLLLLLLLLLLLPRR
                     PAGERIRPRRPPRHAHPRPPLTRWRPSTGYLAAGASPGTLSTTVPTGPGVSCGSRGIC
                     PSGRLRLPRQAQTNQTTTAPPNSQTMAPLKTVGTLGMMDTTGSVLKTVHSSNLPFCGS
                     SHEPDPTLRDPEAMTRRWPWMVSVQANGSHICAGILIASQWVLTVAHCLSQNHVNYIV
                     RAGSPWINQTAGTSSDVPVHRVIINHGYQPRRYWSWVGRAHDIGLLKLKWGLKYSKYV
                     WPICLPGLDYVVEDSSLCTVTGWGYPRANGIWPQFQSLQEKEVSILNSKKCDHFYHKF
                     SRISSLVRIINPQMICASDNNREEFCYEITGEPLVCSSDGTWYLVGMMSWGPGCKKSE
                     APPIFLQVSYYRPWIWDRLSGEPLALPAPSRTLLLAFLLLLILLGTL"
     exon            494..656
                     /gene="Prss50"
                     /gene_synonym="79H19C; Tsp50"
                     /inference="alignment:Splign:2.1.0"
     exon            657..940
                     /gene="Prss50"
                     /gene_synonym="79H19C; Tsp50"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1107
                     /gene="Prss50"
                     /gene_synonym="79H19C; Tsp50"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1455
                     /gene="Prss50"
                     /gene_synonym="79H19C; Tsp50"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 attgtggagg aacctagcgg cgcaatggag ccctggtgcg gggcagaggt ccgtgggcag
       61 ggccctcagg gtccccgtgt gcctggggct tcccgctccc gctcccgcgc ccttctcctg
      121 ctgttgctgc tgctgctgct gctcctgcct cggcggccgg caggtgagcg catccgcccc
      181 cgacgccctc cccggcacgc ccacccgcgg ccacctctga ctcgctggag accttccaca
      241 ggctacttgg ccgcaggagc atccccgggg acgctgtcca ccaccgtccc gaccggaccc
      301 ggggtctcct gtggctccag aggcatctgt ccctcaggca ggcttcgcct tcctcggcaa
      361 gcccagacta accagaccac gacggctcca ccgaactcac agaccatggc tccactgaaa
      421 acggtgggga ctctcggcat gatggacact actgggtccg ttcttaagac ggttcattcc
      481 agcaacctcc ccttctgtgg ctcctcccac gagccagacc ctactctcag ggacccagaa
      541 gccatgactc ggcggtggcc ctggatggtc agcgtgcagg ctaatggctc acacatctgt
      601 gctggcatcc ttatcgcttc ccagtgggtg ctgaccgtgg cccattgctt gagccagaac
      661 catgttaact acatagtgag ggcggggagc ccgtggatta atcagacggc aggaaccagc
      721 tcagatgtgc cggtccatcg agtcatcata aaccatggct accaacccag acggtactgg
      781 tcatgggttg gccgggccca tgacatcggc cttctcaagc tcaagtgggg gctcaagtac
      841 agtaaatacg tgtggcccat ctgcctgcct ggcctggatt acgtggtgga ggacagttct
      901 ctctgcactg tgacaggctg gggatatccc agggctaacg gcatctggcc ccagttccag
      961 tccctccagg agaaggaagt ctctatcctg aacagcaaga aatgtgatca tttctaccac
     1021 aagttctcca gaatctcctc tctggttcgg atcatcaacc ctcagatgat ctgtgcctcg
     1081 gacaacaaca gggaggagtt ctgctatgag ataactggcg agcccctggt ctgctcttca
     1141 gatggcacat ggtacctggt gggaatgatg agctggggcc caggctgcaa gaagagcgag
     1201 gccccaccca tctttctgca ggtctcctac tacaggccct ggatctggga ccggctcagt
     1261 ggggagcccc tggcccttcc agccccatcc aggaccttgc tcctggcttt ccttctgctc
     1321 ctcatccttc tgggcacact gtgacactgc catgtctctc cttccttccc ctcctcctaa
     1381 gtgctgctgt gggggtggcg ctcagcctgc cggaggcggg gaggagctag cagagattaa
     1441 acacttcttt tcccc
//
