LOCUS       NP_666339                439 aa            linear   ROD 30-APR-2024
DEFINITION  probable threonine protease PRSS50 isoform 1 precursor [Mus
            musculus].
ACCESSION   NP_666339 XP_922202
VERSION     NP_666339.2
DBSOURCE    REFSEQ: accession NM_146227.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 439)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 439)
  AUTHORS   Li,J., Niu,C., Ai,H., Li,X., Zhang,L., Lang,Y., Wang,S., Gao,F.,
            Mei,X., Yu,C., Sun,L., Huang,Y., Zheng,L., Wang,G., Sun,Y.,
            Yang,X., Song,Z. and Bao,Y.
  TITLE     TSP50 Attenuates DSS-Induced Colitis by Regulating TGF-beta
            Signaling Mediated Maintenance of Intestinal Mucosal Barrier
            Integrity
  JOURNAL   Adv Sci (Weinh) 11 (11), e2305893 (2024)
   PUBMED   38189580
  REMARK    GeneRIF: TSP50 Attenuates DSS-Induced Colitis by Regulating
            TGF-beta Signaling Mediated Maintenance of Intestinal Mucosal
            Barrier Integrity.
REFERENCE   3  (residues 1 to 439)
  AUTHORS   Scovell,J.M., Bournat,J.C., Szafran,A.T., Solis,M., Moore,J.,
            Rivera,A., Chen,C.H., Zhang,J., Wilken,N., Seth,A. and Jorgez,C.J.
  TITLE     PRSS50 is a testis protease responsible for proper sperm tail
            formation and function
  JOURNAL   Development 148 (8) (2021)
   PUBMED   33913480
  REMARK    GeneRIF: PRSS50 is a testis protease responsible for proper sperm
            tail formation and function.
REFERENCE   4  (residues 1 to 439)
  AUTHORS   Ai,H.H., Liu,B., Yang,M.T., Zuo,Q.Q., Song,Z.B., Bao,Y.L.,
            Sun,L.G., Zhou,L. and Li,Y.X.
  TITLE     Expression and effects of TSP50 in mouse embryo and cardiac myocyte
            development
  JOURNAL   Biochem Biophys Res Commun 502 (2), 283-288 (2018)
   PUBMED   29842883
  REMARK    GeneRIF: TSP50 expression at embryonic day 6.5 (E6.5), day 8.5
            (E8.5) and day 10.5 (E10.5) was increasingly enhanced, However, the
            expression of TSP50 decreased gradually in the development and
            differentiation of cardiac myocyte from E12.5 to postnatal (P0).
REFERENCE   5  (residues 1 to 439)
  AUTHORS   Harten,S.K., Oey,H., Bourke,L.M., Bharti,V., Isbel,L., Daxinger,L.,
            Faou,P., Robertson,N., Matthews,J.M. and Whitelaw,E.
  TITLE     The recently identified modifier of murine metastable epialleles,
            Rearranged L-Myc Fusion, is involved in maintaining epigenetic
            marks at CpG island shores and enhancers
  JOURNAL   BMC Biol 13, 21 (2015)
   PUBMED   25857663
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 439)
  AUTHORS   Zhou,L., Bao,Y.L., Zhang,Y., Wu,Y., Yu,C.L., Huang,Y.X., Sun,Y.,
            Zheng,L.H. and Li,Y.X.
  TITLE     Knockdown of TSP50 inhibits cell proliferation and induces
            apoptosis in P19 cells
  JOURNAL   IUBMB Life 62 (11), 825-832 (2010)
   PUBMED   21086474
  REMARK    GeneRIF: results showed that downregulation of TSP50 expression in
            P19 embryonal carcinoma stem cells not only reduced cell
            proliferation, colony formation, and migration but also induced
            cell apoptosis
REFERENCE   7  (residues 1 to 439)
  AUTHORS   Huang,Y., Wang,Y., Wang,M., Sun,B., Li,Y., Bao,Y., Tian,K. and
            Xu,H.
  TITLE     Differential methylation of TSP50 and mTSP50 genes in different
            types of human tissues and mouse spermatic cells
  JOURNAL   Biochem Biophys Res Commun 374 (4), 658-661 (2008)
   PUBMED   18662669
  REMARK    GeneRIF: These findings suggested that DNA methylation might
            regulate the TSP50 and mTSP50 gene expressions in different types
            of tissues and spermatic cells.
REFERENCE   8  (residues 1 to 439)
  AUTHORS   Cho,K.I., Suh,J.G., Lee,J.W., Hong,S.H., Kang,T.C., Oh,Y.S. and
            Ryoo,Z.Y.
  TITLE     The circling mouse (C57BL/6J-cir) has a 40-kilobase genomic
            deletion that includes the transmembrane inner ear (tmie) gene
  JOURNAL   Comp Med 56 (6), 476-481 (2006)
   PUBMED   17219777
REFERENCE   9  (residues 1 to 439)
  AUTHORS   Xu,H.P., Yuan,L., Shan,J. and Feng,H.
  TITLE     Localization and expression of TSP50 protein in human and rodent
            testes
  JOURNAL   Urology 64 (4), 826-832 (2004)
   PUBMED   15491742
  REMARK    GeneRIF: TSP50 may perform a critical biologic function in
            mammalian spermatogenesis. It may be a new type of threonine
            protease whose linear catalytic structure is similar to many serine
            proteases, except for a Thr-for-Ser residual catalytic site
            substitution.
REFERENCE   10 (residues 1 to 439)
  AUTHORS   Mitchem,K.L., Hibbard,E., Beyer,L.A., Bosom,K., Dootz,G.A.,
            Dolan,D.F., Johnson,K.R., Raphael,Y. and Kohrman,D.C.
  TITLE     Mutation of the novel gene Tmie results in sensory cell defects in
            the inner ear of spinner, a mouse model of human hearing loss DFNB6
  JOURNAL   Hum Mol Genet 11 (16), 1887-1898 (2002)
   PUBMED   12140191
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139378.4.
            
            On or before Dec 7, 2005 this sequence version replaced
            XP_922202.1, NP_666339.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045168.1, BC150686.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 60.79 cM"
     Protein         1..439
                     /product="probable threonine protease PRSS50 isoform 1
                     precursor"
                     /note="testes-specific protease 50; testis-specific
                     protease-like protein 50; probable threonine protease
                     PRSS50; protease, serine 50"
                     /calculated_mol_wt=43622
     sig_peptide     1..47
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=5073
     Region          1..22
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BLH5.1)"
     mat_peptide     48..439
                     /product="Probable threonine protease PRSS50.
                     /id=PRO_0000415813"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BLH5.1)"
                     /calculated_mol_wt=43622
     Region          48..130
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BLH5.1)"
     Site            116
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BLH5.1)"
     Region          177..407
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            187
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BLH5.1)"
     Site            order(207,260,364)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            226
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BLH5.1)"
     Site            order(358,383,385)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     Site            416..436
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BLH5.1)"
     CDS             1..439
                     /gene="Prss50"
                     /gene_synonym="79H19C; Tsp50"
                     /coded_by="NM_146227.4:25..1344"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS23577.1"
                     /db_xref="GeneID:235631"
                     /db_xref="MGI:MGI:2447303"
ORIGIN      
        1 mepwcgaevr gqgpqgprvp gasrsrsral llllllllll lprrpageri rprrpprhah
       61 prppltrwrp stgylaagas pgtlsttvpt gpgvscgsrg icpsgrlrlp rqaqtnqttt
      121 appnsqtmap lktvgtlgmm dttgsvlktv hssnlpfcgs shepdptlrd peamtrrwpw
      181 mvsvqangsh icagiliasq wvltvahcls qnhvnyivra gspwinqtag tssdvpvhrv
      241 iinhgyqprr ywswvgrahd igllklkwgl kyskyvwpic lpgldyvved sslctvtgwg
      301 yprangiwpq fqslqekevs ilnskkcdhf yhkfsrissl vriinpqmic asdnnreefc
      361 yeitgeplvc ssdgtwylvg mmswgpgckk seappiflqv syyrpwiwdr lsgeplalpa
      421 psrtlllafl lllillgtl
//
