LOCUS       NM_023333               2142 bp    mRNA    linear   ROD 19-NOV-2024
DEFINITION  Mus musculus serine protease 3B (Prss3b), mRNA.
ACCESSION   NM_023333
VERSION     NM_023333.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Orekhova,A., Nemeth,B.C., Jancso,Z., Geisz,A., Mosztbacher,D.,
            Demcsak,A. and Sahin-Toth,M.
  TITLE     Evolutionary expansion of polyaspartate motif in the activation
            peptide of mouse cationic trypsinogen limits autoactivation and
            protects against pancreatitis
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 321 (6), G719-G734 (2021)
   PUBMED   34643096
REFERENCE   3  (bases 1 to 2142)
  AUTHORS   Mosztbacher,D. and Sahin-Toth,M.
  TITLE     Mouse model suggests limited role for human mesotrypsin in
            pancreatitis
  JOURNAL   Pancreatology 21 (2), 342-352 (2021)
   PUBMED   33526384
REFERENCE   4  (bases 1 to 2142)
  AUTHORS   Jancso,Z. and Sahin-Toth,M.
  TITLE     Mutation That Promotes Activation of Trypsinogen Increases Severity
            of Secretagogue-Induced Pancreatitis in Mice
  JOURNAL   Gastroenterology 158 (4), 1083-1094 (2020)
   PUBMED   31751559
REFERENCE   5  (bases 1 to 2142)
  AUTHORS   Geisz,A. and Sahin-Toth,M.
  TITLE     A preclinical model of chronic pancreatitis driven by trypsinogen
            autoactivation
  JOURNAL   Nat Commun 9 (1), 5033 (2018)
   PUBMED   30487519
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2142)
  AUTHORS   Xue,L., Cai,J.Y., Ma,J., Huang,Z., Guo,M.X., Fu,L.Z., Shi,Y.B. and
            Li,W.X.
  TITLE     Global expression profiling reveals genetic programs underlying the
            developmental divergence between mouse and human embryogenesis
  JOURNAL   BMC Genomics 14, 568 (2013)
   PUBMED   23961710
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2142)
  AUTHORS   Nemeth,B.C., Wartmann,T., Halangk,W. and Sahin-Toth,M.
  TITLE     Autoactivation of mouse trypsinogens is regulated by chymotrypsin C
            via cleavage of the autolysis loop
  JOURNAL   J Biol Chem 288 (33), 24049-24062 (2013)
   PUBMED   23814066
REFERENCE   8  (bases 1 to 2142)
  AUTHORS   Sah,R.P., Dudeja,V., Dawra,R.K. and Saluja,A.K.
  TITLE     Cerulein-induced chronic pancreatitis does not require intra-acinar
            activation of trypsinogen in mice
  JOURNAL   Gastroenterology 144 (5), 1076-1085 (2013)
   PUBMED   23354015
REFERENCE   9  (bases 1 to 2142)
  AUTHORS   Dawra,R., Sah,R.P., Dudeja,V., Rishi,L., Talukdar,R., Garg,P. and
            Saluja,A.K.
  TITLE     Intra-acinar trypsinogen activation mediates early stages of
            pancreatic injury but not inflammation in mice with acute
            pancreatitis
  JOURNAL   Gastroenterology 141 (6), 2210-2217 (2011)
   PUBMED   21875495
REFERENCE   10 (bases 1 to 2142)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK008460.1, AK008695.1 and AK007943.1.
            
            On Jul 21, 2005 this sequence version replaced NM_023333.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK008695.1, AK008460.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AK008460.1         2-3
            3-83                AK008695.1         2-82
            84-890              AK008460.1         85-891
            891-1714            AK008695.1         890-1713
            1715-1725           AK007943.1         415-425
            1726-2142           AK008695.1         1725-2141
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 19.1 cM"
     gene            1..2142
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /note="serine protease 3B"
                     /db_xref="GeneID:67373"
                     /db_xref="MGI:MGI:1914623"
     exon            1..71
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..775
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /EC_number="3.4.21.4"
                     /note="protease, serine 3B; cationic trypsinogen (isoform
                     T7)"
                     /codon_start=1
                     /product="trypsinogen 7 precursor"
                     /protein_id="NP_075822.3"
                     /db_xref="CCDS:CCDS20043.1"
                     /db_xref="GeneID:67373"
                     /db_xref="MGI:MGI:1914623"
                     /translation="MKTLIFLAFLGAAVALPLDDDDDKIVGGYTCQRNALPYQVSLNS
                     GYHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNIDALEGGEQFIDAAKIIRHPNYNA
                     NTYNNDIMLIKLKTAATLNSRVSTVALPRSCPSAGTRCLVSGWGNTLSSGTNYPSLLQ
                     CLDAPVLSDSSCTSSYPGKITSNMFCLGFLEGGKDSCQGDSGGPVVCNGQLQGVVSWG
                     YGCAQRGKPGVYTKVCKYVNWIQQTIAAN"
     exon            72..234
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /inference="alignment:Splign:2.1.0"
     exon            235..488
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /inference="alignment:Splign:2.1.0"
     exon            489..625
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /inference="alignment:Splign:2.1.0"
     exon            626..2142
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      873
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /note="major polyA site"
     regulatory      984..989
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /note="hexamer: ATTAAA"
     polyA_site      1007
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
     regulatory      2103..2108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
                     /note="hexamer: AATAAA"
     polyA_site      2122
                     /gene="Prss3b"
                     /gene_synonym="2210010C04Rik; T7"
ORIGIN      
        1 ctattgactg ctctagccac agtgagcaac catgaagacc ttaatcttcc ttgccttcct
       61 tggagctgct gttgctctcc ccctggatga tgatgatgac aaaattgttg gaggctacac
      121 ctgccagagg aatgctctcc cctaccaggt gtctctgaac agcggctacc atttttgtgg
      181 aggctcactc atcaattccc agtgggttgt ttcagccgct cactgctaca aatcccgaat
      241 tcaggtgcgc ctgggagaac acaacattga tgcccttgag ggtggtgagc aattcattga
      301 tgcagctaaa atcatccgcc accccaacta taatgcaaac acctataaca atgacatcat
      361 gttgattaag ctgaagacag ctgccaccct caactctcga gtgtccactg tcgctctgcc
      421 aagatcttgt ccatcagctg gtactaggtg tcttgtgtct ggctggggca acaccctgag
      481 ctctggcacc aactaccctt cactccttca gtgtcttgat gctcctgtcc tctctgacag
      541 ttcttgcaca agttcttacc caggcaagat cactagcaac atgttctgtc tgggcttcct
      601 ggagggcgga aaggactcct gccagggtga ctctggtggc cccgtagtct gcaatggaca
      661 actccagggt gttgtttcct ggggttatgg ctgtgctcag agaggaaaac ctggtgtcta
      721 caccaaggtg tgcaagtatg tgaactggat tcagcagacc atcgctgcca actaaatgcc
      781 tttagctctt cataagccct ctctttgttg tcaaatccac cttccttcta tgccctaaac
      841 cactatcaag agaaaaacat tttgttttta ccacatctgt ctatgaatgg ttcttgcata
      901 ataacgaact ttctcttgaa attgctgcca ataagaaaat atatacagtg ttctactatt
      961 ttgtgaagat aattagaaag gatattaaat atttcattca aacctaattt gctagtttat
     1021 taatatagct ggcacacctt agttgaatat ttggctatga tatatgcttt cttatgcccc
     1081 aatgtggtag acctagtgtt aattatttgt aatagattat gatacaaaaa atgcagagat
     1141 aaaagcaata gctaaaagtc agggaaatgg ctcatactgg aatggagatg aagtcaaagc
     1201 tgccagcatt cctaggtcat ttccatttcc atgacagaat gctatttctt ctatgttttt
     1261 cttcagtcaa tccaaaactc tattgtgttg tgtaaaatat taaaaagtac aatccatgct
     1321 atgctggcag ggcctggaga gctggctggc caggcactgg caggcatggc cggcctgttc
     1381 tcatcttgtg gagacgctct gactcagagc tctgaaatct ctccacccag ctcactaagt
     1441 tcccactggt gggtcattac caggccaatt cccacagact tttgggtgca cttcttgcaa
     1501 acccacgctc tgccacttta cctttctctc tgcatgaagg aaaacactac ataaacatag
     1561 ttcagaatca ttgggaactc aatcactgcg tacaactaac ataatttaca agccattcta
     1621 agttaaatcc tctggaagca gatccaaatg gatccaccac ctgaaacgag gtcccctcta
     1681 tctatatttt tgcctcatct ccagccctcc cactcactct cttctcctcc actgtccctc
     1741 tgcccacccc ggaagtccca cctacttgcc cagtgattgg tttctttatt cattagaagg
     1801 gtcacatgac atcccaagta tttgattcac tcctctttcc agtcagttcc tcttggggaa
     1861 gttgaattag catcaaaata cagacagcac cagggctatc cacaacacta ctacacctac
     1921 ctttccaagg attcacactc taatatttcc tcttgttttt ttaatggaat gttgttttaa
     1981 taacaaatac tgtgtatgga gataatctgg cctatctttt tctcaaccac acctcaaaca
     2041 ccaatttaaa tgatgcatta actaaaatat aagccaaaaa taaatgaaat cagtttctct
     2101 aaaataaaca tgttaaacca gagttcataa atctctggat cc
//
