LOCUS       NM_008939               2607 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus serine protease 12 neurotrypsin (motopsin) (Prss12),
            mRNA.
ACCESSION   NM_008939
VERSION     NM_008939.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Miyata,S., Tsuda,M. and Mitsui,S.
  TITLE     Overexpression of Motopsin, an Extracellular Serine Protease
            Related to Intellectual Disability, Promotes Adult Neurogenesis and
            Neuronal Responsiveness in the Dentate Gyrus
  JOURNAL   Mol Neurobiol 61 (8), 4929-4948 (2024)
   PUBMED   38153682
  REMARK    GeneRIF: Overexpression of Motopsin, an Extracellular Serine
            Protease Related to Intellectual Disability, Promotes Adult
            Neurogenesis and Neuronal Responsiveness in the Dentate Gyrus.
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Hashimoto,J., Fujita,E., Tanimoto,K., Kondo,S. and
            Matsumoto-Miyai,K.
  TITLE     Effects of Cardiac Glycoside Digoxin on Dendritic Spines and Motor
            Learning Performance in Mice
  JOURNAL   Neuroscience 541, 77-90 (2024)
   PUBMED   38278474
REFERENCE   3  (bases 1 to 2607)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2607)
  AUTHORS   Doherty,R., MacLeod,B.L., Nelson,M.M., Ibrahim,M.M.H., Borges,B.C.,
            Jaradat,N.W., Finneran,M.C., Giger,R.J. and Corfas,G.
  TITLE     Identification of in vivo roles of ErbB4-JMa and its direct nuclear
            signaling using a novel isoform-specific knock out mouse
  JOURNAL   Sci Rep 12 (1), 17267 (2022)
   PUBMED   36241655
  REMARK    Erratum:[Sci Rep. 2022 Dec 1;12(1):20748. doi:
            10.1038/s41598-022-25021-8.. PMID: 36456684]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2607)
  AUTHORS   Miyata,S., Kashio,T., Tsuchiya,K. and Mitsui,S.
  TITLE     Motopsin deficiency imparts partial insensitivity to
            doxorubicin-induced hippocampal impairments in adult mice
  JOURNAL   Neurosci Lett 763, 136181 (2021)
   PUBMED   34416345
REFERENCE   6  (bases 1 to 2607)
  AUTHORS   Gu,G., Wells,J.M., Dombkowski,D., Preffer,F., Aronow,B. and
            Melton,D.A.
  TITLE     Global expression analysis of gene regulatory pathways during
            endocrine pancreatic development
  JOURNAL   Development 131 (1), 165-179 (2004)
   PUBMED   14660441
REFERENCE   7  (bases 1 to 2607)
  AUTHORS   Simon,R. and Lufkin,T.
  TITLE     Postnatal lethality in mice lacking the Sax2 homeobox gene
            homologous to Drosophila S59/slouch: evidence for positive and
            negative autoregulation
  JOURNAL   Mol Cell Biol 23 (24), 9046-9060 (2003)
   PUBMED   14645517
REFERENCE   8  (bases 1 to 2607)
  AUTHORS   Iijima,N., Tanaka,M., Mitsui,S., Yamamura,Y., Yamaguchi,N. and
            Ibata,Y.
  TITLE     Expression of a serine protease (motopsin PRSS12) mRNA in the mouse
            brain: in situ hybridization histochemical study
  JOURNAL   Brain Res Mol Brain Res 66 (1-2), 141-149 (1999)
   PUBMED   10095086
REFERENCE   9  (bases 1 to 2607)
  AUTHORS   Yamamura,Y., Yamashiro,K., Tsuruoka,N., Nakazato,H., Tsujimura,A.
            and Yamaguchi,N.
  TITLE     Molecular cloning of a novel brain-specific serine protease with a
            kringle-like structure and three scavenger receptor cysteine-rich
            motifs
  JOURNAL   Biochem Biophys Res Commun 239 (2), 386-392 (1997)
   PUBMED   9344839
REFERENCE   10 (bases 1 to 2607)
  AUTHORS   Gschwend,T.P., Krueger,S.R., Kozlov,S.V., Wolfer,D.P. and
            Sonderegger,P.
  TITLE     Neurotrypsin, a novel multidomain serine protease expressed in the
            nervous system
  JOURNAL   Mol Cell Neurosci 9 (3), 207-219 (1997)
   PUBMED   9245503
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110240.8 and AC124005.13.
            
            On Jun 16, 2009 this sequence version replaced NM_008939.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D89871.1, BC031429.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-724               AC110240.8         43144-43867         c
            725-875             AC110240.8         25893-26043         c
            876-1054            AC110240.8         12625-12803         c
            1055-1196           AC110240.8         11074-11215         c
            1197-1393           AC110240.8         7910-8106           c
            1394-1535           AC110240.8         5216-5357           c
            1536-1741           AC110240.8         3618-3823           c
            1742-1820           AC110240.8         1146-1224           c
            1821-1943           AC124005.13        88394-88516         c
            1944-2224           AC124005.13        74460-74740         c
            2225-2607           AC124005.13        73416-73798         c
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 54.58 cM"
     gene            1..2607
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /note="serine protease 12 neurotrypsin (motopsin)"
                     /db_xref="GeneID:19142"
                     /db_xref="MGI:MGI:1100881"
     exon            1..724
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     CDS             247..2532
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /note="neurotrypsin; serine protease 12; brain-specific
                     serine protease 3; protease, serine 12 neurotrypsin
                     (motopsin)"
                     /codon_start=1
                     /product="neurotrypsin precursor"
                     /protein_id="NP_032965.1"
                     /db_xref="CCDS:CCDS17817.1"
                     /db_xref="GeneID:19142"
                     /db_xref="MGI:MGI:1100881"
                     /translation="MALARCVLAVILGALSVVARADPVSRSPLHRPHPSPPRSQHAHY
                     LPSSRRPPRTPRFPLPLRIPAAQRPQVLSTGHTPPTIPRRCGAGESWGNATNLGVPCL
                     HWDEVPPFLERSPPASWAELRGQPHNFCRSPDGSGRPWCFYRNAQGKVDWGYCDCGQG
                     PALPVIRLVGGNSGHEGRVELYHAGQWGTICDDQWDNADADVICRQLGLSGIAKAWHQ
                     AHFGEGSGPILLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSCVPLTDGVIRLA
                     GGKSTHEGRLEVYYKGQWGTVCDDGWTEMNTYVACRLLGFKYGKQSSVNHFDGSNRPI
                     WLDDVSCSGKEVSFIQCSRRQWGRHDCSHREDVGLTCYPDSDGHRLSPGFPIRLVDGE
                     NKKEGRVEVFVNGQWGTICDDGWTDKHAAVICRQLGYKGPARARTMAYFGEGKGPIHM
                     DNVKCTGNEKALADCVKQDIGRHNCRHSEDAGVICDYLEKKASSSGNKEMLSSGCGLR
                     LLHRRQKRIIGGNNSLRGAWPWQASLRLRSAHGDGRLLCGATLLSSCWVLTAAHCFKR
                     YGNNSRSYAVRVGDYHTLVPEEFEQEIGVQQIVIHRNYRPDRSDYDIALVRLQGPGEQ
                     CARLSTHVLPACLPLWRERPQKTASNCHITGWGDTGRAYSRTLQQAAVPLLPKRFCKE
                     RYKGLFTGRMLCAGNLQEDNRVDSCQGDSGGPLMCEKPDESWVVYGVTSWGYGCGVKD
                     TPGVYTRVPAFVPWIKSVTSL"
     exon            725..875
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1054
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1196
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1393
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1535
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1741
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1820
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1943
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2224
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     exon            2225..2607
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2555..2560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /note="hexamer: AGTAAA"
     polyA_site      2567
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
                     /note="major polyA site"
     polyA_site      2602
                     /gene="Prss12"
                     /gene_synonym="Bssp-3; motopsin"
ORIGIN      
        1 cgagggtggg gtggaggtcg gactccgggc tacagagctc ctggcgctca tcgcctctgg
       61 cgctccagcc tttgcttcgc ggggctgacc ctttgggtcc cggtgtgatc ctccagctgc
      121 cccgggggct gggacagcag ggcggcggcg cgagcgtggg agggggctct aggactctgc
      181 cggccccgcc ccgccccctc cgcggggacc cggagcccag catggaccac actcggcgcc
      241 gcagccatgg cgctcgcccg ctgcgtgctg gctgtgattt taggggcact gtctgtagtg
      301 gcccgcgctg atccggtctc gcgctctccc cttcaccgcc cgcatccgtc cccaccgcgt
      361 tcccaacacg cgcactacct tcccagctcg cggcggccac ccaggacccc gcgcttcccg
      421 ctcccgctgc ggatccccgc tgcccagcgc ccgcaggtcc tcagcaccgg gcacacgccc
      481 ccgacgattc cacgccgctg cggggcagga gagtcgtggg gcaatgccac caacctcggc
      541 gtcccgtgtc tacactggga cgaggtgccg cccttcctgg agcggtcgcc cccggccagt
      601 tgggctgagc tgcgagggca gccgcacaac ttctgccgga gcccggatgg ctcgggcaga
      661 ccttggtgct tctatcggaa tgcccagggc aaagtagact ggggctactg cgattgtggt
      721 caaggcccgg cgttgcccgt cattcgcctt gttggtggga acagtgggca tgaaggtcga
      781 gtggagctgt accacgctgg ccagtggggg accatctgtg acgaccaatg ggacaatgca
      841 gacgcagacg tcatctgtag gcagctgggg ctcagtggca ttgccaaagc atggcatcag
      901 gcacattttg gggaaggatc tggcccaata ttgttggatg aagtacgctg caccggaaac
      961 gagctgtcaa ttgagcaatg tccaaagagt tcctggggcg aacataactg tggccataaa
     1021 gaagatgctg gagtgtcttg tgttcctcta acagatggtg tcatcagact ggcaggagga
     1081 aaaagtaccc atgaaggtcg cctggaggtc tactacaagg ggcagtgggg gacagtctgt
     1141 gatgatggct ggactgagat gaacacatac gtggcttgtc gactgctggg atttaaatac
     1201 ggcaaacagt cctctgtgaa ccattttgat ggcagcaaca ggcccatatg gctggatgac
     1261 gtcagctgct caggaaaaga agtcagcttc attcagtgtt ccaggagaca gtggggaagg
     1321 catgactgca gccatagaga agatgtgggc ctcacctgct atcctgacag cgatggacat
     1381 aggctttctc caggttttcc catcagacta gtggatggag agaataagaa ggaaggacga
     1441 gtggaggttt ttgtcaatgg ccaatgggga acaatctgcg atgacggatg gaccgataag
     1501 catgcagctg tgatctgccg gcagcttggc tataagggtc ctgccagagc aaggactatg
     1561 gcttattttg gggaaggaaa aggccccatc cacatggata atgtgaagtg cacaggaaat
     1621 gagaaggccc tggctgactg tgtcaaacaa gacattggaa ggcacaactg ccgccacagt
     1681 gaggatgcag gagtcatctg tgactattta gagaagaaag catcaagtag tggtaataaa
     1741 gagatgctct catctggatg tggactgagg ttactgcacc gtcggcagaa acggatcatt
     1801 ggtgggaaca attctttaag gggtgcctgg ccttggcagg cttccctcag gctgaggtcg
     1861 gcccatggag acggcaggct gctttgtgga gctacccttc tgagtagctg ctgggtcctg
     1921 acagctgcac actgcttcaa aaggtacgga aacaactcga ggagctatgc agttcgagtt
     1981 ggggattatc atactctggt accagaggag tttgaacaag aaataggggt tcaacagatt
     2041 gtgattcaca ggaactacag gccagacaga agcgactatg acattgccct ggttagattg
     2101 caaggaccag gggagcaatg tgccagacta agcacccacg ttttgccagc ctgtttacct
     2161 ctatggagag agaggccaca gaaaacagcc tccaactgtc acataacagg atggggagac
     2221 acaggtcgtg cctactcaag aactctacaa caagctgctg tgcctctgtt acccaagagg
     2281 ttttgtaaag agaggtacaa gggactattt actgggagaa tgctctgtgc tgggaacctc
     2341 caagaagaca accgtgtgga cagctgccag ggagacagtg gaggaccact catgtgtgaa
     2401 aagcctgatg agtcctgggt tgtgtatggg gtgacttcct gggggtatgg atgtggagtc
     2461 aaagacactc ctggagttta taccagagtc cccgcctttg taccttggat aaaaagtgtc
     2521 accagtctgt aacttatgga aagctcaaga aaatagtaaa acagtaacca ttcagtcttc
     2581 atacttggca ccatgccaga aatgaca
//
