LOCUS       NM_178695               2846 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus proline rich Gla (G-carboxyglutamic acid) 4
            (transmembrane) (Prrg4), mRNA.
ACCESSION   NM_178695
VERSION     NM_178695.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK038703.1 and AK035530.1.
            
            On Mar 14, 2009 this sequence version replaced NM_178695.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK035530.1, AK038703.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN01164134,
                                           SAMN01164137 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1086              AK038703.1         1-1086
            1087-2846           AK035530.1         913-2672
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 54.98 cM"
     gene            1..2846
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /note="proline rich Gla (G-carboxyglutamic acid) 4
                     (transmembrane)"
                     /db_xref="GeneID:228413"
                     /db_xref="MGI:MGI:2442211"
     exon            1..305
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /inference="alignment:Splign:2.1.0"
     exon            306..430
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /inference="alignment:Splign:2.1.0"
     CDS             328..1008
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /note="transmembrane gamma-carboxyglutamic acid protein 4;
                     proline-rich Gla protein 4; proline-rich
                     gamma-carboxyglutamic acid protein 4"
                     /codon_start=1
                     /product="transmembrane gamma-carboxyglutamic acid protein
                     4 precursor"
                     /protein_id="NP_848810.3"
                     /db_xref="CCDS:CCDS16493.1"
                     /db_xref="GeneID:228413"
                     /db_xref="MGI:MGI:2442211"
                     /translation="MFPLLIVLSQLPRLTLAVPHCIRSLKDSEHAPEEVFASKEAANI
                     FMHRRLLNNRFDLELFTPGDLERECYEEFCSYEEAREILGDDENTIKFWQTYSIKGPT
                     TGSDVNKEKIDVMSLLTGLIVAGVFLVIFGLVGYYVCLTKCKRRPYPSSSANYTRTAR
                     YTPSIVFRSPEEAVLSPSTSSEDAGLPSYEQAVALTRKHSVSPPPPYPGPARGFRVFK
                     KSMSLPSH"
     exon            431..594
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /inference="alignment:Splign:2.1.0"
     exon            595..643
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /inference="alignment:Splign:2.1.0"
     exon            644..776
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /inference="alignment:Splign:2.1.0"
     exon            777..2846
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1124..1129
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /note="hexamer: AATAAA"
     polyA_site      1147
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /note="major polyA site"
     polyA_site      1299
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /note="major polyA site"
     regulatory      1411..1416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /note="hexamer: AATAAT"
     polyA_site      1449
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /note="major polyA site"
     regulatory      1798..1803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
                     /note="hexamer: AACAAA"
     polyA_site      1826
                     /gene="Prrg4"
                     /gene_synonym="9930111I18Rik; TMG4"
ORIGIN      
        1 gagaagggct ggggaccgag agcgggaggt ggagcgctgc caggtgacct cctcgtccgg
       61 tagcggtggg agtggctcca gcacacaagc tgggccccgt gcggcgcacg ttccccgagc
      121 gcccctgcgc ggatcctggg cgcctctttt gcgtcctggt gtcgccttgg agccgcgaag
      181 agctaggctc cgactgtcgc cgagatccag aggcgtccgc gactcagggc caggccacct
      241 ctcgggccaa gcggataact cggttcccgc ccaggggctg ctggaacatg cgcccgggga
      301 cacagtttgt ttgacagttg ctagactatg tttccacttc tgatcgtact cagccagctg
      361 cccagactta ccctcgcagt tcctcattgt atcagaagcc taaaggattc cgaacatgcc
      421 ccagaagaag tctttgcgtc aaaagaagca gcaaacatct ttatgcaccg ccgcctcctg
      481 aacaataggt ttgatctaga actcttcact cctggggacc tggagagaga gtgctatgag
      541 gagttctgta gttatgaaga agccagagag attttggggg atgatgaaaa tacgatcaaa
      601 ttttggcaga cttattcaat taaaggacca accacaggat cagatgttaa caaagagaaa
      661 attgatgtta tgagccttct gactggattg attgttgctg gagtattctt ggttattttt
      721 ggcttagttg gctactatgt atgtctcacc aagtgtaaaa ggcggccata tccaagctct
      781 tcagccaact acacaagaac ggccagatac acaccctcca ttgttttccg aagccctgag
      841 gaagctgtct tatctccatc aacatcttca gaggatgcag gactaccttc ctatgaacag
      901 gcagtagcac tgaccagaaa gcacagtgtc tcaccaccac ctccctatcc tgggcctgca
      961 agaggattta gggtatttaa aaagtccatg tccctcccat ctcactaagc ctacctggct
     1021 gccttgctgg aagacattca ttcatgtggt tgaataattt gttctccctg gtggggaaaa
     1081 aaaaaagatt tgcttgttca gcttcttatg acacattgca aggaataaag gaatgatgca
     1141 tacagaagag aattcaccac agctctgctt tcgtcttgga cttgaactgg atcgctatca
     1201 tcttggtaag aaacttcaac tccattacct tgaaattaag aagaaagtgc cttttgcaat
     1261 gtatactgaa ctggttcaaa aatgtttgtt gtagctggat aatgtggtgt gtgcctacaa
     1321 acactcagga ggcttcgagg ggcgggatga gagttcaagg ccctgggctc tgtcaagacc
     1381 ctgtctgaaa acacagtttg ttattagtag aataattaga attagccaac caggaactgt
     1441 ccgatcttag atcatgagat tgggtcttct cttccttagg atttcttttg ggctaaaaat
     1501 acttcataga atttgatgct ttgctgtaaa tctgtggcct tgatataatc ccttggtttt
     1561 ctgttctaat tagtgcagag cattcatcaa atttacaggt cttagttttt cactatcaat
     1621 cacagttcaa tggtgtcaag aggttttgtt tgtttttggt tttttttttt ggtttttgtt
     1681 ttttttttgt ttttggtttt ttaattacta ttcatggact aaggggatgc ttcacttgga
     1741 gagtgctggt catacaaata gaaggacctg agttcaatcc tcaataccca tgtttgaaac
     1801 aaaccaacca acaacaacaa acaaaatgaa caaacaaaca aacaaacaaa acaactttga
     1861 gtacttgggt gcacacctgt aatctcagca ctgtggggag gactcaggag gacccctgag
     1921 cctggtggct agccacagga actgaaccag gaacctctaa attcagtctc aaaatttaag
     1981 ataggtgggt gaatgccttt aatcccggca ctcgatacgc agagatctct ctgtgagttc
     2041 aaggtcagcc tattctacat tttaagttct gggacagcta caaagcctgc ctgagaaaaa
     2101 acaaacaaac aaacaaacaa aaagcaaaaa acaaggcttc gaggtagggg tatatgtctt
     2161 taatcacagc actcagaaga cagaggcaga aggatctctg agttcgaagc caccttggtt
     2221 tatatagcta gttccaggac acccagggct acacaaagaa accctgtctc aaataccatt
     2281 taaaaaaatt aaggtggaga gcagttgaag aagataccaa tgtcaacttc tggcctctac
     2341 atgcacaagc ttgcatgcat gagcatacat gcacatacaa tattgagcta gaagtttagt
     2401 aatagaatgg tagaggcaag tggttgaaca tttacttcat gtccatgagg ccctggcttt
     2461 gaggtcctca gcagtaccaa ccagaaagcc tttgcatctt agatgcatgg gtaggctttg
     2521 tttttttttt ctcattctgt ttgttggtgt gtgtgtgtgt gtgtgagtgt gtgtgtgtgt
     2581 gtgtgtgtct gtgtgctcat gcgtgagcat gctggagata gaagcaaagc atcatatgtg
     2641 ctaagcacat gctgtacaat taagccaaac tctgtctccc ttgattctcc gagtacattt
     2701 taacacacag aaaacacatc atagtcttat cagatacttg tcataatcat ataaatggcc
     2761 agttcttccc taataatgta gaagagctca gtgaaaaacc ccatgctcag catacgtgca
     2821 agaacctggg tcagaccccc agcacc
//
