LOCUS       NM_022999               1403 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus proline-rich Gla (G-carboxyglutamic acid) polypeptide
            2 (Prrg2), transcript variant 1, mRNA.
ACCESSION   NM_022999 XM_133452
VERSION     NM_022999.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1403)
  AUTHORS   Jolliffe,C.N., Harvey,K.F., Haines,B.P., Parasivam,G. and Kumar,S.
  TITLE     Identification of multiple proteins expressed in murine embryos as
            binding partners for the WW domains of the ubiquitin-protein ligase
            Nedd4
  JOURNAL   Biochem J 351 Pt 3 (Pt 3), 557-565 (2000)
   PUBMED   11042109
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC126256.4.
            
            On Mar 27, 2023 this sequence version replaced NM_022999.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025098.1, SRR17253014.3609506.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               AC126256.4         105761-105904       c
            145-296             AC126256.4         104914-105065       c
            297-478             AC126256.4         104378-104559       c
            479-518             AC126256.4         104145-104184       c
            519-660             AC126256.4         100916-101057       c
            661-789             AC126256.4         98692-98820         c
            790-1403            AC126256.4         97757-98370         c
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.1 cM"
     gene            1..1403
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /note="proline-rich Gla (G-carboxyglutamic acid)
                     polypeptide 2"
                     /db_xref="GeneID:65116"
                     /db_xref="MGI:MGI:1929596"
     exon            1..144
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            145..296
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..808
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; transmembrane gamma-carboxyglutamic acid
                     protein 2; proline-rich Gla protein 2; NEDD4 WW
                     domain-binding protein 1; proline-rich
                     gamma-carboxyglutamic acid protein 2"
                     /codon_start=1
                     /product="transmembrane gamma-carboxyglutamic acid protein
                     2 isoform 1 precursor"
                     /protein_id="NP_075375.1"
                     /db_xref="CCDS:CCDS21227.1"
                     /db_xref="GeneID:65116"
                     /db_xref="MGI:MGI:1929596"
                     /translation="MRGRPSLLLVYMGLATCLDTSPHREQNQVLDIFLDAPEAQSFLV
                     GRRRFPRANHWDLELLTPGNLERECLEERCSWEEAREYFEDNTLTERFWESYTYNGKG
                     GRGRVDVAGLAVGLTSGILLIVLAGLGAFWYLHYRRRRLRGQESCLQETGLIIPLSPQ
                     TPQSPPLPPGLPTYEQALAASGVHDAPPPPYSSLRRPH"
     exon            297..478
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            479..518
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            519..660
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            661..789
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            790..1403
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1355..1360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /note="hexamer: AATAAA"
     regulatory      1382..1387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /note="hexamer: AATAAA"
     polyA_site      1396
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
     polyA_site      1403
                     /gene="Prrg2"
                     /gene_synonym="1600014G02Rik; 2510041M06Rik; N4WBP1"
                     /note="major polyA site"
ORIGIN      
        1 agtagccggc tcgggcctag taacccagga ggagctcccc tcctctcctc ccctcccctc
       61 ttgcctaccc ctttgcacag ctggctactc tgacccagag ggccttcgtt aaagaagaga
      121 ggcagagctc ccagccctgg ccagagtgcg ggcagggagt cctggcctcc taactccgct
      181 gcagcatttg tcatctggga gtccacacag tatgaggggc cgtccttccc tgctcttagt
      241 ctatatggga ttggccacct gcctggacac ctcacctcac agagagcaaa accaagttct
      301 tgacatcttc ctggatgccc cagaggccca gagtttctta gttggccgca ggcgttttcc
      361 tcgtgctaac cactgggacc tggagttgct gacccctggg aacctggaac gggagtgtct
      421 ggaggagagg tgctcctggg aggaggcccg ggagtacttt gaggacaaca cactgacgga
      481 gcgcttctgg gaaagctata cgtacaatgg caagggaggg cgtggacggg tggatgttgc
      541 aggcctggcg gtgggactga cctctgggat cctcctcatt gtcttggccg gcttgggagc
      601 cttttggtat ctgcattatc gacggcgccg cctcagaggc caggagtcct gtctgcaaga
      661 gaccgggctc atcatcccct tgagtcctca gacgcctcaa tctccaccct tgcccccagg
      721 ccttcccacc tatgagcagg ctttagcagc ctccggggtg cacgacgcac ctccgccccc
      781 ttactccagc ctcagaaggc ctcactgaag acctgcttgc agcgcgggct ccctgaactg
      841 tgcccctgat tcatagcgga ttccggaagt cgccagcttc ttcgacgact gggcttcagt
      901 gtgtccgtgg gggagtcagg gtcgtcggac ccgaggtcta ccatgacaca cgtgtttccg
      961 ccgggcacgc atacacgcac gtccctgacc atcctgttgc cgagttggtg cccccggccc
     1021 tccagtgacc cccccccaca cttcgtctga gcagtggccc tgcctcagaa tgggcagacc
     1081 ttttaggaaa ctcggatcag acgtgactcg gctgtcactc cactggtgcc gcggggcacg
     1141 tacgcgcagg gaaccaggcc cttgcacacg cctgacttcg tgtatttccc cagtcgggca
     1201 catgggcggt ttgtaatcca gtataaaggt ggggggcgca tttgcaatgt gctgtaagcg
     1261 gggtgtcacg cttgtgttgc acccgggtaa atggagttga tctgagctgc cctctgctta
     1321 aaaacccttt gggaaaggaa ggggggatgg aagaaataaa aaattgaaac aatgagaaaa
     1381 aaataaaaat gcaaaagaac tga
//
