LOCUS       NM_146061               1835 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus proline rich 5 (renal) (Prr5), transcript variant 1,
            mRNA.
ACCESSION   NM_146061 XM_001002278
VERSION     NM_146061.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   3  (bases 1 to 1835)
  AUTHORS   Gan,X., Wang,J., Wang,C., Sommer,E., Kozasa,T., Srinivasula,S.,
            Alessi,D., Offermanns,S., Simon,M.I. and Wu,D.
  TITLE     PRR5L degradation promotes mTORC2-mediated PKC-delta
            phosphorylation and cell migration downstream of Galpha12
  JOURNAL   Nat Cell Biol 14 (7), 686-696 (2012)
   PUBMED   22609986
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1835)
  AUTHORS   Laplante,M. and Sabatini,D.M.
  TITLE     mTOR signaling in growth control and disease
  JOURNAL   Cell 149 (2), 274-293 (2012)
   PUBMED   22500797
  REMARK    Review article
REFERENCE   5  (bases 1 to 1835)
  AUTHORS   Pearce,L.R., Sommer,E.M., Sakamoto,K., Wullschleger,S. and
            Alessi,D.R.
  TITLE     Protor-1 is required for efficient mTORC2-mediated activation of
            SGK1 in the kidney
  JOURNAL   Biochem J 436 (1), 169-179 (2011)
   PUBMED   21413931
REFERENCE   6  (bases 1 to 1835)
  AUTHORS   Johnstone,C.N., Castellvi-Bel,S., Chang,L.M., Sung,R.K.,
            Bowser,M.J., Pique,J.M., Castells,A. and Rustgi,A.K.
  TITLE     PRR5 encodes a conserved proline-rich protein predominant in
            kidney: analysis of genomic organization, expression, and mutation
            status in breast and colorectal carcinomas
  JOURNAL   Genomics 85 (3), 338-351 (2005)
   PUBMED   15718101
REFERENCE   7  (bases 1 to 1835)
  AUTHORS   Sarbassov,D.D., Ali,S.M., Kim,D.H., Guertin,D.A., Latek,R.R.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     Rictor, a novel binding partner of mTOR, defines a
            rapamycin-insensitive and raptor-independent pathway that regulates
            the cytoskeleton
  JOURNAL   Curr Biol 14 (14), 1296-1302 (2004)
   PUBMED   15268862
REFERENCE   8  (bases 1 to 1835)
  AUTHORS   Shan,Z., Haaf,T. and Popescu,N.C.
  TITLE     Identification and characterization of a gene encoding a putative
            mouse Rho GTPase activating protein gene 8, Arhgap8
  JOURNAL   Gene 303, 55-61 (2003)
   PUBMED   12559566
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            W99003.1, AK081231.1, BY059925.1, BE653785.1, BF458163.1,
            AV002324.1, BG079574.2 and AK028831.1.
            
            On Jan 10, 2007 this sequence version replaced NM_146061.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK005634.1, AF482997.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 W99003.1           1-5
            6-331               AK081231.1         2-327
            332-332             BY059925.1         304-304
            333-1070            AK081231.1         329-1066
            1071-1356           AK081231.1         1067-1352
            1357-1466           BE653785.1         379-488
            1467-1678           BF458163.1         14-225
            1679-1703           AV002324.1         63-87
            1704-1834           BG079574.2         382-512
            1835-1835           AK028831.1         955-955
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 39.95 cM"
     gene            1..1835
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /note="proline rich 5 (renal)"
                     /db_xref="GeneID:109270"
                     /db_xref="MGI:MGI:1924714"
     exon            1..322
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..1349
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein observed with Rictor-1; proline-rich protein 5"
                     /codon_start=1
                     /product="proline-rich protein 5 isoform 1"
                     /protein_id="NP_666173.4"
                     /db_xref="CCDS:CCDS27711.1"
                     /db_xref="GeneID:109270"
                     /db_xref="MGI:MGI:1924714"
                     /translation="MRTLRRLKFMSSPSLSDLGKREPGAAGTDERGTQQRRACANATW
                     NSIHNGVIAVFQRKGLPDQELFILNEGVRQLLKTELGSFFTEYLQNQLLTKGMVILRD
                     KIRFYEGQKLLDSLAETWDFFFSDVLPTLQAIFYPVQGKEPSVRQLALLHFRNTITLS
                     VKLEDALARSHARVPPAIAQMLLVLQGVHESRGVTEDYLRLETLIQKVVSPYLGTYGL
                     YSNEGPCTHSCILEKRFLRRSRSGDILAKNPVVRSKSYNTPLLNPVAEHEAEGTAASG
                     TSIRRHSVSEMTSCPEPQGFVDTPGQGPSGTFRSSPTPHSGPCPSRLYPPAHSPEQGP
                     GHGSPSTSSPETLVDQILESADSDSEGIFIDFGRGSRSSVSDFEAPGGRPSVV"
     exon            323..403
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /inference="alignment:Splign:2.1.0"
     exon            404..452
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /inference="alignment:Splign:2.1.0"
     exon            453..510
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /inference="alignment:Splign:2.1.0"
     exon            511..602
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /inference="alignment:Splign:2.1.0"
     exon            603..743
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /inference="alignment:Splign:2.1.0"
     exon            744..879
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1835
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1816..1821
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /note="hexamer: AATAAA"
     polyA_site      1835
                     /gene="Prr5"
                     /gene_synonym="Arhgap8; C030017C09Rik; Protor-1; Protor1"
                     /note="major polyA site"
ORIGIN      
        1 cttttgcgct ccgagcgctc cacgctgagc cgccgcgagc catggttcac agggccccgc
       61 gctgggagtg ctgccccggg cgggggcgca ggacccgggc gggaccccac ggggtcgccg
      121 ggcttgctgc tagccgccga ctatcgtcgt aggggacgcg ggggcgcgtg tgggccgggg
      181 tgagcatgag gactctccgc aggttgaagt tcatgagttc gcccagcctc agtgacctgg
      241 gcaagaggga gccgggcgcg gcgggtacgg acgagcgggg cacgcagcag cgccgggcct
      301 gcgccaacgc cacctggaac agtatccata acggagtgat cgcggtcttc cagcgcaagg
      361 ggctgcccga ccaggagctc ttcatcctca acgagggcgt ccggcagctg ctgaagacgg
      421 agctggggtc cttcttcact gagtacctgc agaaccagct gctcacgaag ggcatggtga
      481 ttcttcgtga caagattcgc ttctacgaag gacagaagct gctggactca ttggcggaga
      541 cctgggattt cttcttcagt gatgtgctgc caaccctgca ggccatcttc tatcctgtgc
      601 agggcaagga gccgtcggtg cgccagctcg ccctgctgca tttccggaac accatcaccc
      661 tcagcgtgaa gctggaggac gcgctggccc gctctcacgc gcgtgttccc cctgccatcg
      721 cacagatgct gctggtactg cagggggtac atgagtccag gggtgtgact gaagactatc
      781 tgcgcctcga gacactgatt cagaaggtgg tgtcgccgta cctgggcacc tatggtctct
      841 attccaatga aggaccttgt acccattcct gtatcctcga gaaacgattc ctacgccgct
      901 cccgctctgg ggatatccta gccaagaacc cagtggtgcg ctccaaaagc tacaacactc
      961 ccctgctcaa tcctgtggcc gagcatgaag cagaaggcac agcagccagc ggcacgagca
     1021 tccgcagaca ctcagtctcc gagatgacat cttgtccgga gccccagggc tttgtggaca
     1081 cgcctggcca gggcccttcg gggaccttcc ggtcttctcc aacaccccac tcagggcctt
     1141 gccccagcag actatacccc cctgcccatt ccccggaaca gggcccaggc catggctccc
     1201 catccacctc cagcccagag actcttgtgg accagatcct ggagtccgcg gactcagact
     1261 cggaagggat attcattgac tttgggcggg gaagtcgctc cagtgtgtct gactttgagg
     1321 caccgggagg ccggccaagt gttgtgtgat ggcctgagac tagttcatgt ttttactgag
     1381 ccctgtgtgt ttgcgtgcct gtgtatgtgt gtatgtgtgt gtgtgtgtgt gtgtgtgtgt
     1441 gtgtgtgtgt gtgtgtgtgt gtgtgtgaga gagagacttg ttactcttgc cagcccccgt
     1501 cactgcctca agtcacttta tactcccagt ctgttggaaa acccatccac cacccctgct
     1561 cacccggact gattgggacc cccagttggc actgcccaga tgcatactca accacctgga
     1621 ggatggatgg ggtcaccatg tgccctcctg cccaccccac ccttggtgcc cacacttacc
     1681 caggaaaatg tgaaaccagc gggctctgcc tatgccagcc cagcctggcc tccggagtga
     1741 cgggactcaa gccacagtca gacctgcctg cctgcctccc tctgctctgg gtggccacaa
     1801 aataggaagg catgaaataa atttacagcc ccaga
//
