LOCUS       NM_138659               7500 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus pre-mRNA processing factor 8 (Prpf8), mRNA.
ACCESSION   NM_138659
VERSION     NM_138659.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Krausova,M., Kreplova,M., Banik,P., Cvackova,Z., Kubovciak,J.,
            Modrak,M., Zudova,D., Lindovsky,J., Kubik-Zahorodna,A., Palkova,M.,
            Kolar,M., Prochazka,J., Sedlacek,R. and Stanek,D.
  TITLE     Retinitis pigmentosa-associated mutations in mouse Prpf8 cause
            misexpression of circRNAs and degeneration of cerebellar granule
            cells
  JOURNAL   Life Sci Alliance 6 (6), e202201855 (2023)
   PUBMED   37019475
  REMARK    GeneRIF: Retinitis pigmentosa-associated mutations in mouse Prpf8
            cause misexpression of circRNAs and degeneration of cerebellar
            granule cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7500)
  AUTHORS   Xu,G., Li,T., Chen,J., Li,C., Zhao,H., Yao,C., Dong,H., Wen,K.,
            Wang,K., Zhao,J., Xia,Q., Zhou,T., Zhang,H., Gao,P., Li,A. and
            Pan,X.
  TITLE     Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is
            essential for hypoxia-induced mitophagy through regulating ULK1
            mRNA splicing
  JOURNAL   Autophagy 14 (10), 1818-1830 (2018)
   PUBMED   30103670
  REMARK    GeneRIF: Knockdown of PRPF8 significantly impairs mitophagosome
            formation and subsequent mitochondrial clearance through the
            aberrant mRNA splicing of ULK1, which mediates
            macroautophagy/autophagy initiation.
REFERENCE   4  (bases 1 to 7500)
  AUTHORS   Farkas,M.H., Lew,D.S., Sousa,M.E., Bujakowska,K., Chatagnon,J.,
            Bhattacharya,S.S., Pierce,E.A. and Nandrot,E.F.
  TITLE     Mutations in pre-mRNA processing factors 3, 8, and 31 cause
            dysfunction of the retinal pigment epithelium
  JOURNAL   Am J Pathol 184 (10), 2641-2652 (2014)
   PUBMED   25111227
  REMARK    GeneRIF: The mouse retinal pigment epithelium (RPE)is the primary
            cell affected by mutations in the RNA splicing factors (PRPF3,
            PRPF8, and PRPF31), and these changes occur at an early age.
REFERENCE   5  (bases 1 to 7500)
  AUTHORS   Tenin,G., Clowes,C., Wolton,K., Krejci,E., Wright,J.A.,
            Lovell,S.C., Sedmera,D. and Hentges,K.E.
  TITLE     Erbb2 is required for cardiac atrial electrical activity during
            development
  JOURNAL   PLoS One 9 (9), e107041 (2014)
   PUBMED   25269082
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7500)
  AUTHORS   Shu,H., Chen,S., Bi,Q., Mumby,M. and Brekken,D.L.
  TITLE     Identification of phosphoproteins and their phosphorylation sites
            in the WEHI-231 B lymphoma cell line
  JOURNAL   Mol Cell Proteomics 3 (3), 279-286 (2004)
   PUBMED   14729942
REFERENCE   7  (bases 1 to 7500)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 7500)
  AUTHORS   Takahashi,A., Muramatsu,H., Takagi,S., Fujisawa,H., Miyake,Y. and
            Muramatsu,T.
  TITLE     A splicing factor, Prp8: preferential localization in the testis
            and ovary in adult mice
  JOURNAL   J Biochem 129 (4), 599-606 (2001)
   PUBMED   11275560
REFERENCE   9  (bases 1 to 7500)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
REFERENCE   10 (bases 1 to 7500)
  AUTHORS   Hamilton,B.A., Smith,D.J., Mueller,K.L., Kerrebrock,A.W.,
            Bronson,R.T., van Berkel,V., Daly,M.J., Kruglyak,L., Reeve,M.P.,
            Nemhauser,J.L., Hawkins,T.L., Rubin,E.M. and Lander,E.S.
  TITLE     The vibrator mutation causes neurodegeneration via reduced
            expression of PITP alpha: positional complementation cloning and
            extragenic suppression
  JOURNAL   Neuron 18 (5), 711-722 (1997)
   PUBMED   9182797
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ120731.1, AK041017.1, AK144232.1, CK636594.1, CN530184.1,
            CB558040.1, BU510116.1, CO426130.1, CV561799.1 and BP757620.1.
            
            On Oct 6, 2006 this sequence version replaced NM_138659.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC093481.1, AB047391.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-472               CJ120731.1         2-473
            473-2631            AK041017.1         240-2398
            2632-3518           AK144232.1         2392-3278
            3519-4121           CK636594.1         114-716
            4122-4729           CN530184.1         22-629
            4730-5404           CB558040.1         132-806
            5405-5843           BU510116.1         363-801
            5844-6351           CO426130.1         95-602
            6352-7048           CV561799.1         47-743
            7049-7500           BP757620.1         1-452               c
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.92 cM"
     gene            1..7500
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /note="pre-mRNA processing factor 8"
                     /db_xref="GeneID:192159"
                     /db_xref="MGI:MGI:2179381"
     exon            1..415
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     CDS             316..7323
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /note="splicing factor Prp8"
                     /codon_start=1
                     /product="pre-mRNA-processing-splicing factor 8"
                     /protein_id="NP_619600.2"
                     /db_xref="CCDS:CCDS25048.1"
                     /db_xref="GeneID:192159"
                     /db_xref="MGI:MGI:2179381"
                     /translation="MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKR
                     YAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKL
                     LENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRH
                     FKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRK
                     YVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGG
                     PKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHT
                     PNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTP
                     LYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKL
                     LKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLI
                     HRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRL
                     GNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKG
                     PGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFD
                     LELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMIL
                     RYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDG
                     PYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRL
                     NQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPL
                     EKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGE
                     CNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGI
                     IRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLF
                     CRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHD
                     VNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSY
                     EEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNK
                     WNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
                     ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESE
                     FIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGW
                     RVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTY
                     FPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQ
                     VQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQ
                     KETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRW
                     GDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLI
                     QQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTN
                     VYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTA
                     EEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVE
                     KFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDK
                     TTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEI
                     SAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWR
                     VRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVS
                     PPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWIHTQPNESPQLSP
                     QDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNP
                     KGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYH
                     EVHRPSHFLNFALLQEGEVYSADREDLYA"
     exon            416..584
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            585..749
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            750..968
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1181
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1307
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1413
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1604
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1724
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1914
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2034
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2169
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2299
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..2496
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2703
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2867
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..2994
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            2995..3187
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            3188..3375
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            3376..3614
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            3615..3761
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            3762..3972
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            3973..4089
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            4090..4337
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            4338..4517
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            4518..4653
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            4654..4823
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            4824..4953
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            4954..5100
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            5101..5261
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            5262..5453
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            5454..5691
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            5692..5820
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            5821..5934
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            5935..6108
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            6109..6302
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            6303..6542
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            6543..6684
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            6685..6825
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            6826..6965
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            6966..7168
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     exon            7169..7481
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7462..7467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /note="hexamer: AATAAA"
     polyA_site      7481
                     /gene="Prpf8"
                     /gene_synonym="D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l"
                     /note="major polyA site"
ORIGIN      
        1 ggatgagctt ctgagcctgg ttgcgcactt cctggagttg ggaacgcgga gcggacgtcg
       61 tctttccgcc attgctctgc gagcctagcc gctacctgag tgaggggcga caggtgggtg
      121 gtgggcgtct gaggctgagc gcaaggtaat cgagtgggcc gagcggcccg aggagctgcg
      181 ccaggcccgg gtcccgagcg ctcagcccgg ctagagtgca ggccaggggg ctccggacgt
      241 gttccatctg ccccttgtgg gcccgggcct agctcattag ttttggcctg cctctctatc
      301 ccagattctc ccggcatggc cggagtgttt ccttaccgag ggccgggtaa cccggtgccc
      361 ggccctctag ccccgctgcc ggattatatg tcagaggaga agctgcagga gaaagctcga
      421 aaatggcagc agttgcaggc caagcgatat gcagagaagc ggaaatttgg gtttgtggat
      481 gctcagaagg aagacatgcc accagaacat gtcaggaaga tcattcgaga ccatggagac
      541 atgaccaaca ggaagttccg tcatgacaaa agggtctatt tgggagctct taagtacatg
      601 cctcatgcag tcctcaagct ccttgagaac atgcccatgc cttgggagca gatccgggac
      661 gtgcctgtgc tgtaccacat cactggagcc atctcctttg ttaacgagat tccctgggtt
      721 atcgagccgg tttacatctc ccagtggggg tcaatgtgga tcatgatgcg tagagaaaaa
      781 agagacagga ggcatttcaa gaggatgcgt ttcccccctt tcgatgacga ggaaccgccc
      841 ctggattatg ccgacaacat cttagatgtt gagccactgg aagccattca gctagagctg
      901 gacccagagg aggatgcccc tgtgttggac tggttctatg accaccagcc tttgagagac
      961 agtaggaagt acgttaatgg ttccacctac caacgctggc agttcacatt gcctatgatg
     1021 tccacactct accgactggc caatcagctt ctgactgacc ttgtagatga caactacttc
     1081 tatctctttg atctgaaggc cttttttacc tctaaggcac tcaacatggc cattcctggg
     1141 ggacccaaat ttgagcctct tgttcgagac atcaacttac aggatgaaga ctggaatgaa
     1201 tttaacgata ttaataagat catcatccgt cagcctattc gtactgagta caagattgct
     1261 tttccttacc tgtataacaa ccttccacac cacgtccacc tgacctggta ccacactccc
     1321 aacgttgtgt tcatcaagac tgaggatcct gatctgccag ctttctactt tgatcctttg
     1381 atcaacccaa tctcccatag acactcagtc aagagccaag aaccactgcc tgatgatgat
     1441 gaagagtttg aactcccaga gtttgtcgag cccttcctga aggatacacc tctctataca
     1501 gacaacacag ccaatggcat tgccctgctc tgggcaccac ggcccttcaa cctacgctct
     1561 ggtcgtaccc gccgggcctt ggacataccc cttgtcaaga actggtatcg ggagcactgt
     1621 cctgctgggc agcctgtgaa ggttcgagtc tcttatcaga agctgcttaa gtactatgtg
     1681 ctgaatgccc tcaagcaccg gcctcccaaa gcccagaaga agaggtatct gttccgttct
     1741 ttcaaagcta ccaaattctt tcagtccacc aagctggact gggtggaggt agggctgcag
     1801 gtttgccgcc agggctacaa catgctcaac cttctcattc accggaagaa cctcaactat
     1861 ctgcacttgg actacaactt caacctgaag cctgtcaaga cgctcaccac caaggaaaga
     1921 aagaaatccc gttttgggaa tgctttccat ctatgtcggg aagtactgcg tttaaccaag
     1981 ctggttgtgg acagccatgt gcagtatcgc ttgggcaatg tagatgcctt ccagctggca
     2041 gatggtttgc agtatatatt tgcccacgtg ggacagttga caggcatgta tcgatacaaa
     2101 tataagctga tgcggcagat ccgcatgtgc aaggacttga aacatctcat ctattaccgc
     2161 ttcaacacgg gtcctgtagg gaaaggccct ggctgtggtt tctgggctgc tggctggcgg
     2221 gtctggctgt tctttatgcg tggaattacc cctctgctag agcgatggct gggcaacctt
     2281 ctggcccgac agtttgaagg ccgtcactca aagggggtgg caaagacagt aacgaagcag
     2341 cgggtggagt cgcactttga cctggagctc agggctgctg tgatgcacga cattctggac
     2401 atgatgcctg aaggcatcaa gcagaacaag gcccggacaa ttctgcagca cctcagtgaa
     2461 gcctggcgct gctggaaggc caacatccct tggaaggtcc caggactgcc aacacctata
     2521 gagaatatga ttcttcggta cgttaaggcc aaggctgatt ggtggaccaa tactgcccac
     2581 tacaaccggg aacggatccg tcgcggggct actgtagaca agactgtttg caaaaagaac
     2641 ctgggccgcc tcactcgcct gtatctgaag gcagagcagg agcggcagca caattacctg
     2701 aaggatgggc cttacatcac agccgaggag gcagtggccg tgtataccac cactgtgcac
     2761 tggctggaga gccggagatt ctcgcccatc cccttccctc cactctccta caagcatgat
     2821 accaagctgc tcatcttggc tctggagcgt ctcaaggaag cttatagtgt gaagtctagg
     2881 ttaaaccagt ctcagaggga ggagctgggt ctgattgaac aagcctatga caacccccat
     2941 gaggctttgt cccgcatcaa gcgtcacctc ctcacacaga gagcctttaa ggaagtgggc
     3001 attgagttta tggatctcta tagccacctt gtaccagtgt atgatgtgga accactggag
     3061 aaaataactg atgcttacct ggaccagtac ctgtggtatg aagcagataa acgccgtctt
     3121 ttcccaccct ggatcaagcc tgctgacaca gagccacctc cactgcttgt ttacaagtgg
     3181 tgtcaaggca ttaataacct gcaggatgtg tgggagacga gtgagggcga gtgcaacgtc
     3241 atgctggagt ctcggtttga gaagatgtat gagaagattg acctgaccct gcttaacagg
     3301 ctgctgcgac tcattgtgga ccacaacata gctgactaca tgacggccaa gaacaacgta
     3361 gtcatcaact ataaggacat gaaccataca aactcatatg gaatcatcag aggcctgcag
     3421 tttgcctcat tcattgtaca gtactatggc ttggtgatgg atttgcttgt actgggattg
     3481 caccgggcca gtgagatggc tgggcctcct cagatgccaa atgactttct cagtttccag
     3541 gacatagcca ctgaggctgc acacccaatc cgactcttct gtagatacat tgatcgcatc
     3601 catattttct tcaggttcac agcagatgag gctcgggatc tgatccagcg ttacctgaca
     3661 gaacacccag accccaacaa tgaaaacatt gttggctaca ataacaagaa atgctggccc
     3721 cgagatgccc gaatgcggct catgaagcat gatgtcaact tgggtcgggc agtgttctgg
     3781 gacatcaaaa accggctgcc acgatcggtg actacagtac agtgggagaa tagctttgtg
     3841 tctgtgtaca gtaaagacaa ccccaacctg ctattcaaca tgtgtggctt tgagtgccgc
     3901 atcctgccta agtgccgtac cagctatgaa gagttcaccc acaaggacgg ggtctggaac
     3961 ctacagaatg aggttaccaa ggagcggaca gctcagtgct tcctgcgtgt ggatgatgag
     4021 tcaatgcaac gcttccataa ccgtgtgcgg cagattctga tggcctctgg ctccaccacg
     4081 ttcaccaaga ttgtgaataa gtggaacaca gctctcatcg gccttatgac atattttcga
     4141 gaggctgtgg tgaatacaca ggaactcctg gacttattgg tgaagtgtga aaacaagatc
     4201 cagacacgta tcaagattgg attgaactcc aagatgccta gccggttccc gcctgttgta
     4261 ttctacaccc ctaaggagct gggtgggctt ggcatgctct cgatgggcca tgtgcttatc
     4321 ccccagtctg acctcaggtg gtccaagcag acagatgtgg gcatcacaca cttccgttca
     4381 ggaatgagtc atgaagaaga ccaactgatt cccaacttgt accgctacat acagccatgg
     4441 gagagcgagt tcattgactc ccagcgggtc tgggctgagt atgcactcaa gagacaagag
     4501 gctattgctc agaacagacg cttgacttta gaagatttag aagattcatg ggatcgaggc
     4561 attccccgga tcaataccct cttccagaaa gaccggcaca cattggctta tgataaaggc
     4621 tggcgtgtcc gaactgactt taagcagtac caggttttga agcagaaccc cttttggtgg
     4681 acacatcagc ggcatgatgg caagctttgg aacctcaata actaccgcac agacatgatt
     4741 caggcgctgg gtggtgtgga gggcattctg gagcacactc tcttcaaggg cacttacttc
     4801 cccacctggg agggtctgtt ctgggagaaa gccagtggct ttgaggagtc catgaagtgg
     4861 aagaagctga ccaatgctca gcgatcagga ttaaaccaga ttcctaatcg tagatttacc
     4921 ctctggtggt ccccaaccat caatcgagcc aatgtctatg tgggcttcca ggtgcagctg
     4981 gacctgacag gaatcttcat gcacggcaag atccccacac tgaagatctc tctcatccag
     5041 atattccgag ctcacttgtg gcagaagatc catgagagca tcgtgatgga cttgtgtcag
     5101 gtctttgacc aggaactgga tgcactggaa attgagacag tgcaaaagga gacaatccat
     5161 ccccgaaagt cctataagat gaactcttcc tgtgcggata tactactctt tgcctcctat
     5221 aagtggaatg tctcccggcc ctcgctgctg gcagactcca aggatgtgat ggacagcacc
     5281 accacacaga agtattggat tgacatccaa ttacgctggg gagactatga ttcccatgat
     5341 attgagcgct atgcccgggc caagttcctg gattacacca cagacaacat gagtatctac
     5401 ccttcgccca caggcgtgct cattgccatt gatctggcct ataatctaca cagtgcctat
     5461 ggaaactggt tcccgggcag caagcctctc atacagcagg ccatggccaa gatcatgaag
     5521 gcaaaccctg cgttgtatgt gttacgtgaa cggatacgca aaggattaca gctgtattca
     5581 tctgagccca ctgagcctta cctgtcctct cagaactatg gtgaactctt ctctaaccag
     5641 attatctggt ttgtggatga caccaatgtc tatagagtaa ctatccacaa gacctttgag
     5701 gggaacttga caactaaacc catcaatggg gccatcttca tcttcaatcc acgaacggga
     5761 cagctgttcc tcaagataat ccacacatct gtatgggctg gacagaagcg tttgggtcag
     5821 ttggccaagt ggaagactgc tgaggaagtg gctgccctga tccgatctct gcctgtggag
     5881 gaacagccta aacagatcat tgtcactcgg aagggcatgc tggatcctct ggaggtccac
     5941 ttactggatt tccccaatat tgtgatcaaa ggatcggagc tccagctgcc cttccaggct
     6001 tgtctcaagg tggagaagtt tggggatctc atccttaaag ccaccgagcc ccagatggtc
     6061 cttttcaacc tctatgatga ctggctcaag actatctcct cctacacggc tttctcccgt
     6121 ctcattctga ttctgcgggc tttgcatgtg aacaatgata gagcaaaggt gatcctgaag
     6181 ccagacaaga ctactgttac ggaaccccac cacatctggc ccacgctgac tgacgaggaa
     6241 tggatcaagg tggaggtaca gctcaaggat ctgatcttgg ctgactacgg caagaaaaac
     6301 aacgtgaatg tggcgtcact gacacagtca gaaattcgag acatcatcct gggaatggag
     6361 atctcggcgc catcgcagca gcggcagcag atagctgaga ttgaaaagca gaccaaggaa
     6421 cagtcccagc tcactgctac acagactcga actgtcaaca agcacggtga cgagattatc
     6481 acttcaacca ctagcaacta tgagacccag accttctcat caaagactga atggagagtc
     6541 agggccatct ctgctgccaa cctgcatctg cggaccaatc atatttatgt ttcatctgat
     6601 gacattaagg agactggtta tacgtacatt cttccgaaaa acgtgcttaa gaagttcatc
     6661 tgcatatctg atcttcgtgc ccaaattgca ggatacctgt atggggtgag ccctccagat
     6721 aaccctcagg tgaaggagat ccgttgtatt gtgatggtgc cgcagtgggg cacgcaccag
     6781 actgtgcacc tgcccagcca gctgccccag catgagtacc tcaaggagat ggaaccctta
     6841 ggttggatcc acactcagcc caatgaatcc ccccagctat caccccaaga tgtcaccact
     6901 catgccaaaa tcatggctga caacccatct tgggatgggg agaagactat tatcatcacg
     6961 tgcagcttca caccaggctc ctgcacactg acagcctaca agctgacacc cagtgggtat
     7021 gagtggggcc ggcagaacac agacaaaggc aacaacccca aaggctacct accctcgcac
     7081 tacgagaggg ttcagatgct cctgtctgac cgattcctcg gcttcttcat ggtccccgcc
     7141 cagtcctcct ggaactacaa ctttatgggt gttcgacatg atcccaacat gaaatacgag
     7201 ctgcagctgg caaaccccaa ggaattctac catgaggtcc acaggccctc ccacttcctc
     7261 aacttcgccc ttctgcagga gggtgaggtc tactctgcag acagagagga cctctatgcc
     7321 tagttacttc cttctgcttc agatccccaa agcctggagc ctcttgaacg ccctccccag
     7381 acaagctgct gacattcagc agtcagcccc tttgcgttct gtgcttgtgt tgttgataga
     7441 ttgtaatcct gaacaaaatg taataaattt tataaatagt gaaaaaaaaa aaaaaaaaaa
//
