LOCUS       NM_008934               1584 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus protein C (Proc), transcript variant 1, mRNA.
ACCESSION   NM_008934 XM_984063
VERSION     NM_008934.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ren,D., Fedorova,J., Davitt,K., Van Le,T.N., Griffin,J.H.,
            Liaw,P.C., Esmon,C.T., Rezaie,A.R. and Li,J.
  TITLE     Activated Protein C Strengthens Cardiac Tolerance to Ischemic
            Insults in Aging
  JOURNAL   Circ Res 130 (2), 252-272 (2022)
   PUBMED   34930019
  REMARK    GeneRIF: Activated Protein C Strengthens Cardiac Tolerance to
            Ischemic Insults in Aging.
            Erratum:[Circ Res. 2022 Jun 24;131(1):e1. doi:
            10.1161/RES.0000000000000556.. PMID: 35737758]
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Xue,M., Lin,H., Zhao,R., Fryer,C., March,L. and Jackson,C.J.
  TITLE     Activated Protein C Protects against Murine Contact Dermatitis by
            Suppressing Protease-Activated Receptor 2
  JOURNAL   Int J Mol Sci 23 (1), 516 (2022)
   PUBMED   35008942
  REMARK    GeneRIF: Activated Protein C Protects against Murine Contact
            Dermatitis by Suppressing Protease-Activated Receptor 2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1584)
  AUTHORS   Cai,X., Biswas,I., Panicker,S.R., Giri,H. and Rezaie,A.R.
  TITLE     Activated protein C inhibits lipopolysaccharide-mediated
            acetylation and secretion of high-mobility group box 1 in
            endothelial cells
  JOURNAL   J Thromb Haemost 17 (5), 803-817 (2019)
   PUBMED   30865333
  REMARK    GeneRIF: APC inhibits LPS-mediated cytoplasmic translocation and
            secretion of HMGB1 in endothelial cells by epigenetic mechanisms
REFERENCE   4  (bases 1 to 1584)
  AUTHORS   Heestermans,M., Ouweneel,A.B., Hassan,J., Kloosterman,M.,
            Reitsma,P.H., Gijbels,M.J.J., van Vlijmen,B.J.M. and van Eck,M.
  TITLE     Predilection of Low Protein C-induced Spontaneous Atherothrombosis
            for the Right Coronary Sinus in Apolipoprotein E deficient mice
  JOURNAL   Sci Rep 8 (1), 15106 (2018)
   PUBMED   30305662
  REMARK    GeneRIF: there is a predilection of low protein C-induced
            spontaneous atherothrombosis in Apoe(-/-) mice for the right
            coronary sinus.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1584)
  AUTHORS   Iwama,A., Zhang,P., Darlington,G.J., McKercher,S.R., Maki,R. and
            Tenen,D.G.
  TITLE     Use of RDA analysis of knockout mice to identify myeloid genes
            regulated in vivo by PU.1 and C/EBPalpha
  JOURNAL   Nucleic Acids Res 26 (12), 3034-3043 (1998)
   PUBMED   9611252
REFERENCE   6  (bases 1 to 1584)
  AUTHORS   Jalbert,L.R., Rosen,E.D., Lissens,A., Carmeliet,P., Collen,D. and
            Castellino,F.J.
  TITLE     Nucleotide structure and characterization of the murine gene
            encoding anticoagulant protein C
  JOURNAL   Thromb Haemost 79 (2), 310-316 (1998)
   PUBMED   9493582
REFERENCE   7  (bases 1 to 1584)
  AUTHORS   Gerlitz,B. and Grinnell,B.W.
  TITLE     Mutation of protease domain residues Lys37-39 in human protein C
            inhibits activation by the thrombomodulin-thrombin complex without
            affecting activation by free thrombin
  JOURNAL   J Biol Chem 271 (37), 22285-22288 (1996)
   PUBMED   8798383
REFERENCE   8  (bases 1 to 1584)
  AUTHORS   Krawczak,M., Wacey,A. and Cooper,D.N.
  TITLE     Molecular reconstruction and homology modelling of the catalytic
            domain of the common ancestor of the haemostatic
            vitamin-K-dependent serine proteinases
  JOURNAL   Hum Genet 98 (3), 351-370 (1996)
   PUBMED   8707309
REFERENCE   9  (bases 1 to 1584)
  AUTHORS   Murakawa,M., Okamura,T., Kamura,T., Kuroiwa,M., Harada,M. and
            Niho,Y.
  TITLE     A comparative study of partial primary structures of the catalytic
            region of mammalian protein C
  JOURNAL   Br J Haematol 86 (3), 590-600 (1994)
   PUBMED   8043441
REFERENCE   10 (bases 1 to 1584)
  AUTHORS   Tada,N., Sato,M., Tsujimura,A., Iwase,R. and Hashimoto-Gotoh,T.
  TITLE     Isolation and characterization of a mouse protein C cDNA
  JOURNAL   J Biochem 111 (4), 491-495 (1992)
   PUBMED   1618739
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY086829.1, DV662175.1,
            AC127347.4, CJ237265.1 and BX639472.1.
            
            On Sep 15, 2015 this sequence version replaced NM_008934.3.
            
            Summary: This gene encodes the vitamin K-dependent protein C, which
            plays a vital role in the anticoagulation pathway. The encoded
            protein undergoes proteolytic processing including activation by
            thrombin-thrombomodulin complex to form the anticoagulant serine
            protease that degrades activated coagulation factors. A complete
            lack of the encoded protein in mice results in severe perinatal
            consumptive coagulopathy in the brain and liver, resulting in death
            within 24 hours after birth. Alternative splicing results in
            multiple transcript variants encoding different isoforms that may
            undergo similar processing to generate the mature protein.
            [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). This isoform (1) may undergo proteolytic processing similar to
            isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC013896.1, AF318182.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                BY086829.1         1-67
            68-98               BY086829.1         69-99
            99-830              DV662175.1         1-732
            831-831             AC127347.4         215925-215925       c
            832-903             DV662175.1         734-805
            904-1064            AC127347.4         214414-214574       c
            1065-1571           CJ237265.1         1-507
            1572-1584           BX639472.1         1-13                c
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 17.94 cM"
     gene            1..1584
                     /gene="Proc"
                     /gene_synonym="PC"
                     /note="protein C"
                     /db_xref="GeneID:19123"
                     /db_xref="MGI:MGI:97771"
     exon            1..77
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..1477
                     /gene="Proc"
                     /gene_synonym="PC"
                     /EC_number="3.4.21.69"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; vitamin K-dependent protein C; blood
                     coagulation factor XIV; autoprothrombin IIA; anticoagulant
                     protein C; inactivator of coagulation factors Va, VIII"
                     /codon_start=1
                     /product="vitamin K-dependent protein C isoform 1
                     precursor"
                     /protein_id="NP_032960.3"
                     /db_xref="GeneID:19123"
                     /db_xref="MGI:MGI:97771"
                     /translation="MALPGRNCSFSLAHACVSSSRMWQFRVFLLLMSTWGISSIPAHP
                     DPVFSSSEHAHQVLRVRRANSFLEEMRPGSLERECMEEICDFEEAQEIFQNVEDTLAF
                     WIKYFDGDQCSAPPLDHQCDSPCCGHGTCIDGIGSFSCSCDKGWEGKFCQQELRFQDC
                     RVNNGGCLHYCLEESNGRRCACAPGYELADDHMRCKSTVNFPCGKLGRWIEKKRKILK
                     RDTDLEDELEPDPRIVNGTLTKQGDSPWQAILLDSKKKLACGGVLIHTSWVLTAAHCV
                     EGTKKLTVRLGEYDLRRRDHWELDLDIKEILVHPNYTRSSSDNDIALLRLAQPATLSK
                     TIVPICLPNNGLAQELTQAGQETVVTGWGYQSDRIKDGRRNRTFILTFIRIPLVARNE
                     CVEVMKNVVSENMLCAGIIGDTRDACDGDSGGPMVVFFRGTWFLVGLVSWGEGCGHTN
                     NYGIYTKVGSYLKWIHSYIGEKGVSLKSQKL"
     exon            78..164
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     exon            165..328
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     exon            329..353
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     exon            354..491
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     exon            492..626
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     exon            627..772
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     exon            773..890
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1581
                     /gene="Proc"
                     /gene_synonym="PC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1557..1562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Proc"
                     /gene_synonym="PC"
                     /note="hexamer: ATTAAA"
     polyA_site      1581
                     /gene="Proc"
                     /gene_synonym="PC"
                     /note="major polyA site"
ORIGIN      
        1 ggttatggat taacctgact cccagactga catggcgcta cctggacgaa attgcagttt
       61 ctccttggcc cacgcctgtg tcagcagctc caggatgtgg caattcagag tcttcctgct
      121 gctcatgtcc acctggggaa tatctagcat accggcccat cctgacccag tgttctccag
      181 cagcgagcat gcccaccagg tgcttcgggt cagacgtgcc aacagcttcc tggaagagat
      241 gcggccaggc agcctggaac gggagtgtat ggaggagatc tgtgacttcg aggaggccca
      301 ggagattttc caaaatgtgg aagacacact ggccttctgg atcaagtact ttgacggtga
      361 ccagtgctcg gctccaccct tggaccacca gtgcgacagc ccatgctgcg ggcatggcac
      421 ttgcatcgac ggcataggca gcttcagctg cagctgcgat aagggctggg agggcaagtt
      481 ctgtcagcag gagttgcgct tccaggactg tcgggtgaac aatggcggct gcttgcacta
      541 ctgcctggag gagagcaatg ggcggcgctg cgcttgtgcc ccgggctatg agctggcaga
      601 cgaccacatg cgctgcaagt ccactgtgaa ttttccatgt gggaaactgg ggaggtggat
      661 agagaagaaa cgcaagatcc tcaaacgaga cacagactta gaagatgaac tggaaccaga
      721 tccaaggata gtcaacggaa cgctgacgaa gcagggtgac agtccttggc aggcaatcct
      781 tctggactcc aagaagaagc tggcctgcgg aggggtgctc atccacactt cctgggtgct
      841 gacggcagcc cactgcgtgg agggcaccaa gaagcttacc gtgaggcttg gtgagtatga
      901 tctgcgacgc agggaccact gggagctgga cctggacatc aaggagatcc tcgtccaccc
      961 taactacacc cggagcagca gtgacaacga cattgctctg ctccgcctag cccagccagc
     1021 cactctctcc aaaaccatag tgcccatctg cctgccgaac aatgggctgg cgcaggagct
     1081 cactcaggct ggccaggaga cagtggtgac aggctggggc tatcaaagcg acagaatcaa
     1141 ggatggcaga aggaaccgca ccttcatcct caccttcatc cgcatccctt tggttgctcg
     1201 aaatgagtgc gtggaggtca tgaagaatgt ggtctcggag aacatgctgt gtgcaggcat
     1261 cattggggac acgagagacg cctgtgatgg tgacagtggg gggcccatgg tggtcttctt
     1321 tcggggtacc tggttcctgg tgggcctggt gagctggggt gagggctgtg ggcacaccaa
     1381 caactatggc atctacacca aagtgggaag ctacctcaaa tggattcaca gttacattgg
     1441 ggaaaagggt gtctccctta agagccagaa gctatagcac ccctccctgc tcacctctgg
     1501 accctagaag tcactcttgg agtaaggctg ggctagtgag taccaagaca gaggacatta
     1561 aaggagcatg caacaaacat aaaa
//
