LOCUS       NM_008933                624 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus protamine 2 (Prm2), mRNA.
ACCESSION   NM_008933
VERSION     NM_008933.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Arevalo,L., Merges,G.E., Schneider,S., Oben,F.E., Neumann,I.S. and
            Schorle,H.
  TITLE     Loss of the cleaved-protamine 2 domain leads to incomplete
            histone-to-protamine exchange and infertility in mice
  JOURNAL   PLoS Genet 18 (6), e1010272 (2022)
   PUBMED   35763544
  REMARK    GeneRIF: Loss of the cleaved-protamine 2 domain leads to incomplete
            histone-to-protamine exchange and infertility in mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  AUTHORS   Ho,U.Y., Feng,C.A., Yeap,Y.Y., Bain,A.L., Wei,Z., Shohayeb,B.,
            Reichelt,M.E., Homer,H., Khanna,K.K., Bowles,J. and Ng,D.C.H.
  TITLE     WDR62 is required for centriole duplication in spermatogenesis and
            manchette removal in spermiogenesis
  JOURNAL   Commun Biol 4 (1), 645 (2021)
   PUBMED   34059773
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 624)
  AUTHORS   Schneider,S., Shakeri,F., Trotschel,C., Arevalo,L., Kruse,A.,
            Buness,A., Poetsch,A., Steger,K. and Schorle,H.
  TITLE     Protamine-2 Deficiency Initiates a Reactive Oxygen Species
            (ROS)-Mediated Destruction Cascade during Epididymal Sperm
            Maturation in Mice
  JOURNAL   Cells 9 (8), 1789 (2020)
   PUBMED   32727081
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 624)
  AUTHORS   Keime,S., Heitland,K., Kumm,S., Schlosser,M., Hroch,N., Holtz,W.
            and Engel,W.
  TITLE     Characterization of four genes encoding basic proteins of the
            porcine spermatid nucleus and close linkage of three of them
  JOURNAL   Biol Chem Hoppe Seyler 373 (5), 261-270 (1992)
   PUBMED   1627265
REFERENCE   5  (bases 1 to 624)
  AUTHORS   Charles,C.H., Abler,A.S. and Lau,L.F.
  TITLE     cDNA sequence of a growth factor-inducible immediate early gene and
            characterization of its encoded protein
  JOURNAL   Oncogene 7 (1), 187-190 (1992)
   PUBMED   1741163
REFERENCE   6  (bases 1 to 624)
  AUTHORS   Engel,W., Keime,S., Kremling,H., Hameister,H. and Schluter,G.
  TITLE     The genes for protamine 1 and 2 (PRM1 and PRM2) and transition
            protein 2 (TNP2) are closely linked in the mammalian genome
  JOURNAL   Cytogenet Cell Genet 61 (2), 158-159 (1992)
   PUBMED   1395729
REFERENCE   7  (bases 1 to 624)
  AUTHORS   Carre-Eusebe,D., Lederer,F., Le,K.H. and Elsevier,S.M.
  TITLE     Processing of the precursor of protamine P2 in mouse. Peptide
            mapping and N-terminal sequence analysis of intermediates
  JOURNAL   Biochem J 277 (Pt 1) (Pt 1), 39-45 (1991)
   PUBMED   1854346
REFERENCE   8  (bases 1 to 624)
  AUTHORS   Elsevier,S.M., Noiran,J. and Carre-Eusebe,D.
  TITLE     Processing of the precursor of protamine P2 in mouse.
            Identification of intermediates by their insolubility in the
            presence of sodium dodecyl sulfate
  JOURNAL   Eur J Biochem 196 (1), 167-175 (1991)
   PUBMED   2001695
REFERENCE   9  (bases 1 to 624)
  AUTHORS   Bellve,A.R., McKay,D.J., Renaux,B.S. and Dixon,G.H.
  TITLE     Purification and characterization of mouse protamines P1 and P2.
            Amino acid sequence of P2
  JOURNAL   Biochemistry 27 (8), 2890-2897 (1988)
   PUBMED   3401454
REFERENCE   10 (bases 1 to 624)
  AUTHORS   Yelick,P.C., Balhorn,R., Johnson,P.A., Corzett,M., Mazrimas,J.A.,
            Kleene,K.C. and Hecht,N.B.
  TITLE     Mouse protamine 2 is synthesized as a precursor whereas mouse
            protamine 1 is not
  JOURNAL   Mol Cell Biol 7 (6), 2173-2179 (1987)
   PUBMED   3600661
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK005729.1 and AV207640.1.
            
            On Sep 11, 2015 this sequence version replaced NM_008933.1.
            
            Summary: Protamines substitute for histones in the chromatin of
            sperm during the haploid phase of spermatogenesis, and are the
            major DNA-binding proteins in the nucleus of sperm in many
            vertebrates. They package the sperm DNA into a highly condensed
            complex in a volume less than 5% of a somatic cell nucleus. Many
            mammalian species have only one protamine (protamine 1); however, a
            few species, including human and mouse, have two. This gene encodes
            protamine 2, which is synthesized as a precursor and then cleaved
            to give rise to a family of protamine 2 peptides. [provided by
            RefSeq, Sep 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BU962013.1, CA464166.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-618               AK005729.1         2-619
            619-624             AV207640.1         199-204
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 5.84 cM"
     gene            1..624
                     /gene="Prm2"
                     /gene_synonym="Prm-2"
                     /note="protamine 2"
                     /db_xref="GeneID:19119"
                     /db_xref="MGI:MGI:97766"
     exon            1..366
                     /gene="Prm2"
                     /gene_synonym="Prm-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..431
                     /gene="Prm2"
                     /gene_synonym="Prm-2"
                     /note="sperm histone P2; sperm protamine P2"
                     /codon_start=1
                     /product="protamine-2"
                     /protein_id="NP_032959.1"
                     /db_xref="CCDS:CCDS27955.1"
                     /db_xref="GeneID:19119"
                     /db_xref="MGI:MGI:97766"
                     /translation="MVRYRMRSPSEGPHQGPGQDHEREEQGQGQGLSPERVEDYGRTH
                     RGHHHHRHRRCSRKRLHRIHKRRRSCRRRRRHSCRHRRRHRRGCRRSRRRRRCRCRKC
                     RRHHH"
     exon            367..624
                     /gene="Prm2"
                     /gene_synonym="Prm-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      603..608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prm2"
                     /gene_synonym="Prm-2"
                     /note="hexamer: AATAAA"
     polyA_site      621
                     /gene="Prm2"
                     /gene_synonym="Prm-2"
                     /note="major polyA site"
ORIGIN      
        1 accagaccat catcaccacc aagagcaggt gggcaggctt tcgtccctcc tcctccaatc
       61 caggtcagct gcagcctcaa tccagaacct cctgatctcc tggcaccatg gttcgctacc
      121 gaatgaggag ccccagtgag ggtccgcacc aggggcctgg acaagaccat gaacgcgagg
      181 agcaggggca ggggcaaggg ctgagcccag agcgcgtaga ggactatggg aggacacaca
      241 ggggccacca ccaccacaga cacaggcgct gctctcgtaa gaggctacat aggatccaca
      301 agaggcgtcg gtcatgcaga aggcggagga gacactcctg ccgccacagg aggcggcatc
      361 gcagaggctg cagaagatcc cgaaggagga ggagatgcag gtgcaggaaa tgtaggaggc
      421 accatcacta agcctcccca ggcctgtcca ttctgcctgg agccaaggaa gtcacttgcc
      481 caaggaatag tcacctgccc aagcaacatc atgtgaggcc acaccaccat tccatgtcga
      541 tgtctgagcc ctgagctgcc aaggagccac gagatctgag tactgagcaa agccacctgc
      601 caaataaagc ttgacacgag attc
//
