LOCUS       NM_016979               4260 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus protein kinase, X-linked (Prkx), transcript variant 1,
            mRNA.
ACCESSION   NM_016979
VERSION     NM_016979.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Piazzon,N., Maisonneuve,C., Guilleret,I., Rotman,S. and
            Constam,D.B.
  TITLE     Bicc1 links the regulation of cAMP signaling in polycystic kidneys
            to microRNA-induced gene silencing
  JOURNAL   J Mol Cell Biol 4 (6), 398-408 (2012)
   PUBMED   22641646
REFERENCE   3  (bases 1 to 4260)
  AUTHORS   Li,X., Iomini,C., Hyink,D. and Wilson,P.D.
  TITLE     PRKX critically regulates endothelial cell proliferation,
            migration, and vascular-like structure formation
  JOURNAL   Dev Biol 356 (2), 475-485 (2011)
   PUBMED   21684272
  REMARK    GeneRIF: The interaction of PRKX with Pin-1, Magi-1 and Bag-3 could
            contribute to the stimulating role of PRKX in angiogenesis.
            Erratum:[Dev Biol. 2013 Sep 15;381(2):502]
REFERENCE   4  (bases 1 to 4260)
  AUTHORS   Li,X., Burrow,C.R., Polgar,K., Hyink,D.P., Gusella,G.L. and
            Wilson,P.D.
  TITLE     Protein kinase X (PRKX) can rescue the effects of polycystic kidney
            disease-1 gene (PKD1) deficiency
  JOURNAL   Biochim Biophys Acta 1782 (1), 1-9 (2008)
   PUBMED   17980165
REFERENCE   5  (bases 1 to 4260)
  AUTHORS   Li,W., Yu,Z.X. and Kotin,R.M.
  TITLE     Profiles of PrKX expression in developmental mouse embryo and human
            tissues
  JOURNAL   J Histochem Cytochem 53 (8), 1003-1009 (2005)
   PUBMED   15879576
REFERENCE   6  (bases 1 to 4260)
  AUTHORS   Blaschke,R.J., Monaghan,A.P., Bock,D. and Rappold,G.A.
  TITLE     A novel murine PKA-related protein kinase involved in neuronal
            differentiation
  JOURNAL   Genomics 64 (2), 187-194 (2000)
   PUBMED   10729225
REFERENCE   7  (bases 1 to 4260)
  AUTHORS   Blaschke,R.J. and Rappold,G.A.
  TITLE     Man to mouse--lessons learned from the distal end of the human X
            chromosome
  JOURNAL   Genome Res 7 (12), 1114-1117 (1997)
   PUBMED   9414316
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK034681.1, AK037141.1, BG074563.2, CJ136116.1, AK143039.1 and
            BE943829.1.
            
            On Jul 26, 2018 this sequence version replaced NM_016979.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.669016.1, AK154447.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1838              AK034681.1         341-2178
            1839-2865           AK037141.1         1855-2881
            2866-3181           BG074563.2         1-316               c
            3182-3690           CJ136116.1         130-638
            3691-4240           AK143039.1         695-1244
            4241-4260           BE943829.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 38.32 cM"
     gene            1..4260
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /note="protein kinase, X-linked"
                     /db_xref="GeneID:19108"
                     /db_xref="MGI:MGI:1309999"
     exon            1..392
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             236..1303
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     putative serine/threonine kinase; serine/threonine-protein
                     kinase PRKX; PKA-related protein kinase; cAMP-dependent
                     protein kinase catalytic subunit PRKX"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase catalytic subunit
                     PRKX isoform 1"
                     /protein_id="NP_058675.1"
                     /db_xref="CCDS:CCDS30248.1"
                     /db_xref="GeneID:19108"
                     /db_xref="MGI:MGI:1309999"
                     /translation="MEPPAGAAATVKDPDHDPVKTKVSAPAADPKPRTSSQKAGHSLQ
                     DWDTIATVGTGTFGRVNLVKEKTGRQYCALKIMSIPDVIRLKQEQHVQNEKAVLKEIN
                     HPFLIKLLWTGHDNRFLYMLMEFVPGGELFTYLRNRGRFSSVASVFYATEIVCAIEYL
                     HSKEIVYRDLKPENILLDREGHIKLTDFGFAKKLVDRTWTLCGTPEYLAPEVIQSKGH
                     GRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILACKIDFPRQLDFTSKDLIKKLL
                     VVDRTRRLGNMKNGAEDIKRHRWFRGVEWESVPQRKLKPPIVPKLSGDGDISNFETYP
                     ESELDKTPSVSDKDLETFKNF"
     exon            393..561
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            562..825
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..945
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1041
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1099
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1177
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1326
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..4246
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4221..4226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /note="hexamer: AATAAA"
     polyA_site      4246
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /note="major polyA site"
ORIGIN      
        1 gcccagccgg cttccgccgg gaatgaagag ggttgcggtt ggctccggtc ccgcggaccc
       61 cacgcgcgtc cgtggagccc tcgggcgact tccatgagcc cctcccatcg ctctgagacc
      121 cgctgagttt gcttccattg tcctctcggc cgcgggctcc ggaacacccg tgcgagccca
      181 ctgctgatgc acagactgcc attgcgccgc tgtcacaccg tgcgtccccc gccccatgga
      241 gccgcccgct ggtgccgcgg cgaccgtaaa agatccagac catgaccccg tgaagaccaa
      301 ggtctcggcg cccgccgcgg accccaaacc caggacatcg tcacagaagg cggggcactc
      361 gttacaagat tgggacacca tagccaccgt gggcactggg acctttggcc gtgtaaacct
      421 ggtgaaggag aagacaggca ggcaatactg tgccttgaag attatgagta tcccagatgt
      481 catccgcctg aaacaggaac agcatgtgca gaatgagaaa gcagtcctga aggaaataaa
      541 ccatcccttc ctcattaaac tgctttggac aggccatgac aaccgcttcc tatacatgtt
      601 gatggagttc gtgcctggtg gtgagctctt cacctacctg cgtaatcgtg gccgattctc
      661 ctcagttgct tcagtcttct acgccacaga gattgtgtgt gccatagaat atctacactc
      721 aaaggaaatc gtatacaggg acctgaagcc tgaaaacatc cttctagaca gggaaggaca
      781 tattaaactg acagacttcg gcttcgccaa gaaactggtg gataggacat ggactctctg
      841 tgggacacca gaatatctcg ccccagaagt cattcagagc aaaggtcatg gaagggctgt
      901 ggactggtgg gcactgggca ttcttatatt cgagatgctg tctgggtttc ccccattttt
      961 cgatgacaac ccgtttggga tttaccagaa aatccttgca tgcaaaatag atttccccag
     1021 acaattggat ttcacctcta aggacctcat caagaagctg ctggtggtgg acaggacccg
     1081 acgcctaggc aacatgaaga atggggcaga agacatcaag cggcaccggt ggttccgagg
     1141 tgtggagtgg gaatctgtgc cacaaagaaa actgaagcca cccattgtgc ccaagttatc
     1201 gggtgatggt gacatctcca actttgagac ttatccagag agtgaattgg acaagacacc
     1261 ttctgtatct gacaaagacc tggaaacatt caaaaatttc tgaggatgag aactcatgtc
     1321 tgaaagatgt atgtgaagac atcgatacca caccatacca caccacagca caacatacca
     1381 caccaatggc cagaaccttt tttgtcgtac gtttccattt cctactatga aactagaagg
     1441 tttggtgtgt gtattccagt tcctgaaaga tcaatacaat catcaccctt ggaacccagc
     1501 ctcatttcaa caactggaaa cctgcccaat gagaattcgc aaaacctgtt aaattgtagg
     1561 ttttctcctt cttcatgagt cttgtgtttg tgctgtgaat tttgtttctt tttttgtttg
     1621 tttgtctttc tactgaactt tttaagtttg aatttgaccc atttggtttt atggctgtca
     1681 gtgtatatgt gcacagaaat tgtgcaaact ggaaattttg gcactttgtg cttgtgacat
     1741 gttcattttt taaagctttc tgaaaaatct ttcaccctac aggcaaaaaa aaaaagggtg
     1801 agatatatgc tttttaaaaa tgcctttcta tgcattttgc aatcatttgt gtacattgtt
     1861 tatttagtta ctgtttgttt atttagttcc tttctggtca ctttaaactt ggaagtcagg
     1921 aagccacatg ccctgcagac ccttattcag atatagcaca aatgtgtggc tttcctccaa
     1981 tcttccagtt tagatgtttt cagtaaaata tcccttccta aagttaagcc agaactctgc
     2041 atacagcatg gcacctgggt gcaaagattg cctttttatg ataatacaaa tgtacccaca
     2101 tccccacaag ggaactttaa ggatccaccc cttaagcccc tccctcctct gttgtaaatg
     2161 gcattttctt ctcaccatct taactttgtt ctcccagcca tgtccgtccg tgaaaactca
     2221 ggttccaggc cgggaacttc tatgaaagtt tgtacagagc tgtatttttg gtgagctttc
     2281 tcagtgcttt ccaatatgca aactaactgg gttatgtacc tcaaacaaac aaacacatac
     2341 aggagataga atgcaggatt ataatcaagt cctgtcagta gatgtttgct attaagtagg
     2401 aaataacatg aaattagcat aggaaatatt gccttaattc taaacacaca tggggagatc
     2461 acacatgcgc acatgcaccc tctgtgtgct cctaaagcac cttgcaatct gactccaatc
     2521 ctaaaaatca aataaaaaac ccttaatcag gctgtaaatc aaatgacact atgcgatgtc
     2581 actacagtga catcagacat catataggaa tcataatttt ctattttaag atttttaatt
     2641 ctgtgtgtac atgtgactgc agtacacaca gccaacaagt gcaccagaga ccctacagct
     2701 ggacttatag gcagttctat gccgccctgc ggatgctgag aaccaaccca acctcgatcc
     2761 tctgcagtcc atgtagttaa catgcaagaa ggggtggtaa ggcaaaagca gccatgaaaa
     2821 agggtgaggg aaatatgtcc tgttcttaac tctgagtcca gggagagttg tgcacccaac
     2881 accttctcat gaaacaatgg aatagttaat aaatttcaaa tttctctggt ggtagcttga
     2941 ttcgaaccaa agtataaaag ataatcacta gcaaataaaa atttaaaaac aaatttctgg
     3001 gctggagaga tggctcagcg gttaagagca gtgactgctc ttccggaggt cctgagttca
     3061 aatcccagca tcaacctggt ggctcacaac tatctgtaat gggatccgat gcccttttct
     3121 gatgtgtcta aagacagcta caatgtactt acatataata aataaataaa tcttaaaaag
     3181 caaaaaaaaa aaaaaggaaa tcccctcttt taaaaaaaaa aaaaacaaat ctctatgctc
     3241 ttcaactaag actgacttta atgtttagta cagataaagt actacgtgct gtggattggt
     3301 gcttgtctca ctaaactagc tgttacatgt tacactgtag aatgtgattt gatggcttaa
     3361 tactgttcac aggaccctct ggctgtgcaa atatcccaac attggaccaa aacataccaa
     3421 tcaccactgt gttgctaggt gaaccctagt gaaaatttag cattgtgtgg gtcttgatgt
     3481 tgaggtgaaa atcacagaaa gggtctcact tctgactgta gatttgaaat atgttgattt
     3541 cctgggattt gtttgtttgt ttttgttatt gttttggatg aagctccgta ggaagcattt
     3601 gatgaacatt caacacctgt cattcttatt tgtcagaaca ctgtaaacca cctgaaactt
     3661 ataatgcaaa gtcaaggttt atgtgtgagt tggtgccccc attaggttgt gtttgttccc
     3721 atcagtttag ggggaggatc tgcttcccaa ctgttaaatg ttactatcaa atctgccttt
     3781 gagtctcaca cccttgcatc tggcctcaca gttatttggg agagggaggt tgctccagtt
     3841 ggttatgtaa tagacatcat gacatcatta tctgtgtgac ttagagaaag tgagcacccc
     3901 agcgctcctg gtcctcacat gtcagactaa aaccccttgt cacctccgtt cttctcagag
     3961 tgtggggctc atcacttgac ctgctataac acaggtccca cgtgcactgt tcaggtgcac
     4021 ccccccccct gctgccgagc aaccgtcagc ttgcctgaaa ttatgcagaa tgaaaaagcc
     4081 tgtttctttt tcctttttta tcctgatgca tattaaaaaa aatcactgtg atttgtgtgt
     4141 atatctctct atatatttgc cccaggaaaa ctttctgctg tctaatttat aaaatactat
     4201 tactgagtcc aggtttgttt aataaaagtt tgtatgtttt aagaaaaaaa aaaaaaaaaa
//
