LOCUS       NM_001411686            4168 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus protein kinase, X-linked (Prkx), transcript variant 2,
            mRNA.
ACCESSION   NM_001411686
VERSION     NM_001411686.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Piazzon,N., Maisonneuve,C., Guilleret,I., Rotman,S. and
            Constam,D.B.
  TITLE     Bicc1 links the regulation of cAMP signaling in polycystic kidneys
            to microRNA-induced gene silencing
  JOURNAL   J Mol Cell Biol 4 (6), 398-408 (2012)
   PUBMED   22641646
REFERENCE   3  (bases 1 to 4168)
  AUTHORS   Li,X., Iomini,C., Hyink,D. and Wilson,P.D.
  TITLE     PRKX critically regulates endothelial cell proliferation,
            migration, and vascular-like structure formation
  JOURNAL   Dev Biol 356 (2), 475-485 (2011)
   PUBMED   21684272
  REMARK    GeneRIF: The interaction of PRKX with Pin-1, Magi-1 and Bag-3 could
            contribute to the stimulating role of PRKX in angiogenesis.
            Erratum:[Dev Biol. 2013 Sep 15;381(2):502]
REFERENCE   4  (bases 1 to 4168)
  AUTHORS   Li,X., Burrow,C.R., Polgar,K., Hyink,D.P., Gusella,G.L. and
            Wilson,P.D.
  TITLE     Protein kinase X (PRKX) can rescue the effects of polycystic kidney
            disease-1 gene (PKD1) deficiency
  JOURNAL   Biochim Biophys Acta 1782 (1), 1-9 (2008)
   PUBMED   17980165
REFERENCE   5  (bases 1 to 4168)
  AUTHORS   Li,W., Yu,Z.X. and Kotin,R.M.
  TITLE     Profiles of PrKX expression in developmental mouse embryo and human
            tissues
  JOURNAL   J Histochem Cytochem 53 (8), 1003-1009 (2005)
   PUBMED   15879576
REFERENCE   6  (bases 1 to 4168)
  AUTHORS   Blaschke,R.J., Monaghan,A.P., Bock,D. and Rappold,G.A.
  TITLE     A novel murine PKA-related protein kinase involved in neuronal
            differentiation
  JOURNAL   Genomics 64 (2), 187-194 (2000)
   PUBMED   10729225
REFERENCE   7  (bases 1 to 4168)
  AUTHORS   Blaschke,R.J. and Rappold,G.A.
  TITLE     Man to mouse--lessons learned from the distal end of the human X
            chromosome
  JOURNAL   Genome Res 7 (12), 1114-1117 (1997)
   PUBMED   9414316
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL714017.11.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR3363657.71899.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-392               AL714017.11        157241-157632       c
            393-561             AL714017.11        147821-147989       c
            562-825             AL714017.11        143731-143994       c
            826-945             AL714017.11        134809-134928       c
            946-1041            AL714017.11        132940-133035       c
            1042-1099           AL714017.11        127042-127099       c
            1100-1248           AL714017.11        125799-125947       c
            1249-4168           AL714017.11        122665-125584       c
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 38.32 cM"
     gene            1..4168
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /note="protein kinase, X-linked"
                     /db_xref="GeneID:19108"
                     /db_xref="MGI:MGI:1309999"
     exon            1..392
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             236..1225
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     putative serine/threonine kinase; serine/threonine-protein
                     kinase PRKX; PKA-related protein kinase; cAMP-dependent
                     protein kinase catalytic subunit PRKX"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase catalytic subunit
                     PRKX isoform 2"
                     /protein_id="NP_001398615.1"
                     /db_xref="GeneID:19108"
                     /db_xref="MGI:MGI:1309999"
                     /translation="MEPPAGAAATVKDPDHDPVKTKVSAPAADPKPRTSSQKAGHSLQ
                     DWDTIATVGTGTFGRVNLVKEKTGRQYCALKIMSIPDVIRLKQEQHVQNEKAVLKEIN
                     HPFLIKLLWTGHDNRFLYMLMEFVPGGELFTYLRNRGRFSSVASVFYATEIVCAIEYL
                     HSKEIVYRDLKPENILLDREGHIKLTDFGFAKKLVDRTWTLCGTPEYLAPEVIQSKGH
                     GRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILACKIDFPRQLDFTSKDLIKKLL
                     VVDRTRRLGNMKPPIVPKLSGDGDISNFETYPESELDKTPSVSDKDLETFKNF"
     exon            393..561
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            562..825
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..945
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1041
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1099
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1248
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..4168
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4143..4148
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /note="hexamer: AATAAA"
     polyA_site      4168
                     /gene="Prkx"
                     /gene_synonym="Pkare; PKX1"
                     /note="major polyA site"
ORIGIN      
        1 gcccagccgg cttccgccgg gaatgaagag ggttgcggtt ggctccggtc ccgcggaccc
       61 cacgcgcgtc cgtggagccc tcgggcgact tccatgagcc cctcccatcg ctctgagacc
      121 cgctgagttt gcttccattg tcctctcggc cgcgggctcc ggaacacccg tgcgagccca
      181 ctgctgatgc acagactgcc attgcgccgc tgtcacaccg tgcgtccccc gccccatgga
      241 gccgcccgct ggtgccgcgg cgaccgtaaa agatccagac catgaccccg tgaagaccaa
      301 ggtctcggcg cccgccgcgg accccaaacc caggacatcg tcacagaagg cggggcactc
      361 gttacaagat tgggacacca tagccaccgt gggcactggg acctttggcc gtgtaaacct
      421 ggtgaaggag aagacaggca ggcaatactg tgccttgaag attatgagta tcccagatgt
      481 catccgcctg aaacaggaac agcatgtgca gaatgagaaa gcagtcctga aggaaataaa
      541 ccatcccttc ctcattaaac tgctttggac aggccatgac aaccgcttcc tatacatgtt
      601 gatggagttc gtgcctggtg gtgagctctt cacctacctg cgtaatcgtg gccgattctc
      661 ctcagttgct tcagtcttct acgccacaga gattgtgtgt gccatagaat atctacactc
      721 aaaggaaatc gtatacaggg acctgaagcc tgaaaacatc cttctagaca gggaaggaca
      781 tattaaactg acagacttcg gcttcgccaa gaaactggtg gataggacat ggactctctg
      841 tgggacacca gaatatctcg ccccagaagt cattcagagc aaaggtcatg gaagggctgt
      901 ggactggtgg gcactgggca ttcttatatt cgagatgctg tctgggtttc ccccattttt
      961 cgatgacaac ccgtttggga tttaccagaa aatccttgca tgcaaaatag atttccccag
     1021 acaattggat ttcacctcta aggacctcat caagaagctg ctggtggtgg acaggacccg
     1081 acgcctaggc aacatgaagc cacccattgt gcccaagtta tcgggtgatg gtgacatctc
     1141 caactttgag acttatccag agagtgaatt ggacaagaca ccttctgtat ctgacaaaga
     1201 cctggaaaca ttcaaaaatt tctgaggatg agaactcatg tctgaaagat gtatgtgaag
     1261 acatcgatac cacaccatac cacaccacag cacaacatac cacaccaatg gccagaacct
     1321 tttttgtcgt acgtttccat ttcctactat gaaactagaa ggtttggtgt gtgtattcca
     1381 gttcctgaaa gatcaataca atcatcaccc ttggaaccca gcctcatttc aacaactgga
     1441 aacctgccca atgagaattc gcaaaacctg ttaaattgta ggttttctcc ttcttcatga
     1501 gtcttgtgtt tgtgctgtga attttgtttc tttttttgtt tgtttgtctt tctactgaac
     1561 tttttaagtt tgaatttgac ccatttggtt ttatggctgt cagtgtatat gtgcacagaa
     1621 attgtgcaaa ctggaaattt tggcactttg tgcttgtgac atgttcattt tttaaagctt
     1681 tctgaaaaat ctttcaccct acaggcaaaa aaaaaaaggg tgagatatat gctttttaaa
     1741 aatgcctttc tatgcatttt gcaatcattt gtgtacattg tttatttagt tactgtttgt
     1801 ttatttagtt cctttctggt cactttaaac ttggaagtca ggaagccaca tgccctgcag
     1861 acccttattc agatatagca caaatgtgtg gctttcctcc aatcttccag tttagatgtt
     1921 ttcagtaaaa tatcccttcc taaagttaag ccagaactct gcatacagca tggcacctgg
     1981 gtgcaaagat tgccttttta tgataataca aatgtaccca catccccaca agggaacttt
     2041 aaggatccac cccttaagcc cctccctcct ctgttgtaaa tggcattttc ttctcaccat
     2101 cttaactttg ttctcccagc catgtccgtc cgtgaaaact caggttccag gccgggaact
     2161 tctatgaaag tttgtacaga gctgtatttt tggtgagctt tctcagtgct ttccaatatg
     2221 caaactaact gggttatgta cctcaaacaa acaaacacat acaggagata gaatgcagga
     2281 ttataatcaa gtcctgtcag tagatgtttg ctattaagta ggaaataaca tgaaattagc
     2341 ataggaaata ttgccttaat tctaaacaca catggggaga tcacacatgc gcacatgcac
     2401 cctctgtgtg ctcctaaagc accttgcaat ctgactccaa tcctaaaaat caaataaaaa
     2461 acccttaatc aggctgtaaa tcaaatgaca ctatgcgatg tcactacagt gacatcagac
     2521 atcatatagg aatcataatt ttctatttta agatttttaa ttctgtgtgt acatgtgact
     2581 gcagtacaca cagccaacaa gtgcaccaga gaccctacag ctggacttat aggcagttct
     2641 atgccgccct gcggatgctg agaaccaacc caacctcgat cctctgcagt ccatgtagtt
     2701 aacatgcaag aaggggtggt aaggcaaaag cagccatgaa aaagggtgag ggaaatatgt
     2761 cctgttctta actctgagtc cagggagagt tgtgcaccca acaccttctc atgaaacaat
     2821 ggaatagtta ataaatttca aatttctctg gtggtagctt gattcgaacc aaagtataaa
     2881 agataatcac tagcaaataa aaatttaaaa acaaatttct gggctggaga gatggctcag
     2941 cggttaagag cagtgactgc tcttccggag gtcctgagtt caaatcccag catcaacctg
     3001 gtggctcaca actatctgta atgggatccg atgccctttt ctgatgtgtc taaagacagc
     3061 tacaatgtac ttacatataa taaataaata aatcttaaaa agcaaaaaaa aaaaaaagga
     3121 aatcccctct tttaaaaaaa aaaaaaacaa atctctatgc tcttcaacta agactgactt
     3181 taatgtttag tacagataaa gtactacgtg ctgtggattg gtgcttgtct cactaaacta
     3241 gctgttacat gttacactgt agaatgtgat ttgatggctt aatactgttc acaggaccct
     3301 ctggctgtgc aaatatccca acattggacc aaaacatacc aatcaccact gtgttgctag
     3361 gtgaacccta gtgaaaattt agcattgtgt gggtcttgat gttgaggtga aaatcacaga
     3421 aagggtctca cttctgactg tagatttgaa atatgttgat ttcctgggat ttgtttgttt
     3481 gtttttgtta ttgttttgga tgaagctccg taggaagcat ttgatgaaca ttcaacacct
     3541 gtcattctta tttgtcagaa cactgtaaac cacctgaaac ttataatgca aagtcaaggt
     3601 ttatgtgtga gttggtgccc ccattaggtt gtgtttgttc ccatcagttt agggggagga
     3661 tctgcttccc aactgttaaa tgttactatc aaatctgcct ttgagtctca cacccttgca
     3721 tctggcctca cagttatttg ggagagggag gttgctccag ttggttatgt aatagacatc
     3781 atgacatcat tatctgtgtg acttagagaa agtgagcacc ccagcgctcc tggtcctcac
     3841 atgtcagact aaaacccctt gtcacctccg ttcttctcag agtgtggggc tcatcacttg
     3901 acctgctata acacaggtcc cacgtgcact gttcaggtgc accccccccc ctgctgccga
     3961 gcaaccgtca gcttgcctga aattatgcag aatgaaaaag cctgtttctt tttccttttt
     4021 tatcctgatg catattaaaa aaaatcactg tgatttgtgt gtatatctct ctatatattt
     4081 gccccaggaa aactttctgc tgtctaattt ataaaatact attactgagt ccaggtttgt
     4141 ttaataaaag tttgtatgtt ttaagaaa
//
