LOCUS       NM_001293650            2086 bp    mRNA    linear   ROD 19-DEC-2022
DEFINITION  Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript
            variant 1, mRNA.
ACCESSION   NM_001293650 XM_006510094
VERSION     NM_001293650.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Man JCK, Mohan RA, Boogaard MVD, Hilvering CRE, Jenkins C, Wakker
            V, Bianchi V, Laat W, Barnett P, Boukens BJ and Christoffels VM.
  TITLE     An enhancer cluster controls gene activity and topology of the
            SCN5A-SCN10A locus in vivo
  JOURNAL   Nat Commun 10 (1), 4943 (2019)
   PUBMED   31666509
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caputo AT, Alonzi DS, Marti L, Reca IB, Kiappes JL, Struwe WB,
            Cross A, Basu S, Lowe ED, Darlot B, Santino A, Roversi P and
            Zitzmann N.
  TITLE     Structures of mammalian ER alpha-glucosidase II capture the binding
            modes of broad-spectrum iminosugar antivirals
  JOURNAL   Proc Natl Acad Sci U S A 113 (32), E4630-E4638 (2016)
   PUBMED   27462106
REFERENCE   3  (bases 1 to 2086)
  AUTHORS   Fedeles,S.V., So,J.S., Shrikhande,A., Lee,S.H., Gallagher,A.R.,
            Barkauskas,C.E., Somlo,S. and Lee,A.H.
  TITLE     Sec63 and Xbp1 regulate IRE1alpha activity and polycystic disease
            severity
  JOURNAL   J Clin Invest 125 (5), 1955-1967 (2015)
   PUBMED   25844898
REFERENCE   4  (bases 1 to 2086)
  AUTHORS   Shin GC, Ahn SH, Choi HS, Kim J, Park ES, Kim DH and Kim KH.
  TITLE     Hepatocystin contributes to interferon-mediated antiviral response
            to hepatitis B virus by regulating hepatocyte nuclear factor 4alpha
  JOURNAL   Biochim Biophys Acta 1842 (9), 1648-1657 (2014)
   PUBMED   24769044
REFERENCE   5  (bases 1 to 2086)
  AUTHORS   Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H,
            Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T,
            Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P,
            Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ,
            Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD,
            Flicek P, Skarnes WC, Mallon AM and Parkinson H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 2086)
  AUTHORS   Li A, Davila S, Furu L, Qian Q, Tian X, Kamath PS, King BF, Torres
            VE and Somlo S.
  TITLE     Mutations in PRKCSH cause isolated autosomal dominant polycystic
            liver disease
  JOURNAL   Am J Hum Genet 72 (3), 691-703 (2003)
   PUBMED   12529853
REFERENCE   7  (bases 1 to 2086)
  AUTHORS   Brule S, Rabahi F, Faure R, Beckers JF, Silversides DW and Lussier
            JG.
  TITLE     Vacuolar system-associated protein-60: a protein characterized from
            bovine granulosa and luteal cells that is associated with
            intracellular vesicles and related to human 80K-H and murine
            beta-glucosidase II
  JOURNAL   Biol Reprod 62 (3), 642-654 (2000)
   PUBMED   10684806
REFERENCE   8  (bases 1 to 2086)
  AUTHORS   Arendt CW, Dawicki W and Ostergaard HL.
  TITLE     Alternative splicing of transcripts encoding the alpha- and
            beta-subunits of mouse glucosidase II in T lymphocytes
  JOURNAL   Glycobiology 9 (3), 277-283 (1999)
   PUBMED   10024665
REFERENCE   9  (bases 1 to 2086)
  AUTHORS   Brady KP, Rowe LB, Her H, Stevens TJ, Eppig J, Sussman DJ, Sikela J
            and Beier DR.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
REFERENCE   10 (bases 1 to 2086)
  AUTHORS   Arendt CW and Ostergaard HL.
  TITLE     Identification of the CD45-associated 116-kDa and 80-kDa proteins
            as the alpha- and beta-subunits of alpha-glucosidase II
  JOURNAL   J Biol Chem 272 (20), 13117-13125 (1997)
   PUBMED   9148925
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY295975.1, AK137661.1, CX207653.1 and AC163623.5.
            
            [WARNING] On Apr 26, 2023 this sequence was replaced by
            NM_001293650.2.
            
            On Jun 12, 2014 this sequence version replaced XM_006510094.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC009816.1, SRR7652917.88996.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                BY295975.1         2-35
            35-1010             AK137661.1         2-977
            1011-1474           CX207653.1         130-593
            1475-2060           AK137661.1         1421-2006
            2061-2086           AC163623.5         80362-80387
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 8.04 cM"
     gene            1..2086
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /note="protein kinase C substrate 80K-H"
                     /db_xref="GeneID:19089"
                     /db_xref="MGI:MGI:107877"
     exon            1..101
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            102..240
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     CDS             162..1748
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; alpha glucosidase II, beta-subunit; glucosidase
                     2 subunit beta; glucosidase II beta-subunit; carbohydrate
                     processing enzyme of the endoplasmic reticulum; protein
                     kinase C substrate 60.1 kDa protein heavy chain;
                     hepatocystin"
                     /codon_start=1
                     /product="glucosidase 2 subunit beta isoform 1 precursor"
                     /protein_id="NP_001280579.1"
                     /db_xref="CCDS:CCDS80967.1"
                     /db_xref="GeneID:19089"
                     /db_xref="MGI:MGI:107877"
                     /translation="MLLLLLLLLPLCWAVEVKRPRGVSLSNHHFYEESKPFTCLDGTA
                     TIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDG
                     TDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLL
                     ELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAF
                     QELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAI
                     RDKYRSEVPPTDIPVPEETEPKEEKPPVLPPTEEEEEEEEEPEEEEEEEEEEEVQGEQ
                     PKEAPPPLQPPQPPSPTEDEKMPPYDEETQAIIDAAQEARSKFEEVERSLKEMEESIR
                     SLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWG
                     SWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMT
                     PAACPEPPPEAPSDGDHDEL"
     exon            241..357
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            358..453
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            454..511
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            512..629
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            630..759
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            760..844
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            845..923
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1010
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1169
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1266
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1336
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1426
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1427..1501
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1601
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1752
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..2086
                     /gene="Prkcsh"
                     /gene_synonym="80K-H; PKCSH"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtgacgtat ccggagcgtt ccgttctctt attctactgc aggaaccgtg gctgctgagc
       61 taacctggtg tggtggctat tgacctttgc ctctacctcg ctgagaggcc atcgcgtatc
      121 ttcctgccgc ataacacact tccggtctcg gcccggacgg gatgctgctg ctgctgctac
      181 tactactacc cctctgttgg gctgtagaag ttaagagacc ccggggcgtt tccctcagca
      241 accatcactt ctatgaagaa tctaaacctt tcacctgttt ggacggcaca gccaccatcc
      301 cattcgatca ggtgaacgac gactactgcg actgtaagga cggttcagat gagcctggca
      361 cagctgcttg tcccaatggc agctttcact gcaccaacac tgggtacaag cccttgtaca
      421 tcctctccag ccgggtcaat gatggggtat gtgactgctg tgatggcaca gacgagtaca
      481 acagcggcac ggtctgcgag aacacctgca gagagaaggg tcgcaaggaa aaagagtccc
      541 tgcagcagct ggcggaagtc acccgtgaag ggttccgcct gaagaagatt ctcattgagg
      601 agtggaagac agcccgggaa gaaaagcaga gtaagcttct tgagcttcag gctggaaaga
      661 agtctctgga agaccaggta gaaacactgc gggcagcgaa agaagaagca gagaggccag
      721 agaaggaggc caaggaccag caccggaagc tgtgggaaga gcagcaagct gctgccaagg
      781 cccggcggga acaggagcgg gcagccagtg ccttccagga acttgacgac aacatggatg
      841 ggatggtctc gctggctgag ttacagactc acccggagct ggacacagat ggagatggag
      901 cgctgtctga ggaggaggcc caggcccttc tcagtggaga cacacagact gacaccacct
      961 ccttctatga ccgtgtctgg gctgccatca gggacaagta ccgctctgag gtcccgccca
     1021 ctgacatacc tgttccggag gagactgagc ccaaagagga aaagccacca gtgttgccac
     1081 ccacagagga ggaggaagag gaggaggagg agccagaaga agaggaggag gaagaggaag
     1141 aggaggaggt gcagggggag cagcccaagg aggctccgcc cccactgcag cccccacagc
     1201 ctcccagccc cacagaggat gagaagatgc cgccctatga tgaggagacc caggccatca
     1261 tcgatgctgc acaggaggcc cggagtaagt ttgaggaagt cgaacggtcc ttgaaagaga
     1321 tggaagagtc catcaggagt ttggaacaag agatctcctt tgatttcggt ccctctggag
     1381 agtttgcata tctctacagc caatgctacg agctcaccac caatgagtac gtctaccggc
     1441 tttgcccctt caaactggtc tcccagaaac ccaaacatgg gggctccccg accagcctgg
     1501 gcacatgggg ctcctgggct ggccctgatc atgacaagtt cagtgcgatg aagtacgagc
     1561 agggcacggg ctgttggcag ggccccaacc gatccaccac agtgcgcctg ctgtgtggca
     1621 aagagactgt ggtgaccagc accacggagc ccagtcgctg tgagtacctc atggagctga
     1681 tgacaccagc agcctgccca gagccgccac cagaagcacc cagtgatggg gaccatgacg
     1741 agctgtagcc tgaacctcaa ggcctgcaac cagtctactg atcctgaagt ctgcagacca
     1801 aggcctgccc accctgggct tcctggccat cctctgggaa gcaggaaccc tgaggggctc
     1861 taagccccac ttctggccca ggcttgccaa gccatgttcc tgaggcccca gtggcctcca
     1921 gagatgaata atgagctcgc ctaccttgga ttcctaaggg gagttagtcc ctgcacccac
     1981 ctcacccctg cctccccggc tggtgggaga taaatggatg acttgacctg tgacctcaga
     2041 acaataaatg tgacttgtcc acattgtgtg gctgtttttt aatttc
//
