LOCUS       NM_008859               3580 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Mus musculus protein kinase C, theta (Prkcq), transcript variant 1,
            mRNA.
ACCESSION   NM_008859
VERSION     NM_008859.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Gaspar,R.C., Lyu,K., Hubbard,B.T., Leitner,B.P., Luukkonen,P.K.,
            Hirabara,S.M., Sakuma,I., Nasiri,A., Zhang,D., Kahn,M., Cline,G.W.,
            Pauli,J.R., Perry,R.J., Petersen,K.F. and Shulman,G.I.
  TITLE     Distinct subcellular localisation of intramyocellular lipids and
            reduced PKCepsilon/PKCtheta; activity preserve muscle insulin
            sensitivity in exercise-trained mice
  JOURNAL   Diabetologia 66 (3), 567-578 (2023)
   PUBMED   36456864
  REMARK    GeneRIF: Distinct subcellular localisation of intramyocellular
            lipids and reduced PKCepsilon/PKCtheta; activity preserve muscle
            insulin sensitivity in exercise-trained mice.
REFERENCE   3  (bases 1 to 3580)
  AUTHORS   Wang,N., Langfelder,P., Stricos,M., Ramanathan,L., Richman,J.B.,
            Vaca,R., Plascencia,M., Gu,X., Zhang,S., Tamai,T.K., Zhang,L.,
            Gao,F., Ouk,K., Lu,X., Ivanov,L.V., Vogt,T.F., Lu,Q.R.,
            Morton,A.J., Colwell,C.S., Aaronson,J.S., Rosinski,J., Horvath,S.
            and Yang,X.W.
  TITLE     Mapping brain gene coexpression in daytime transcriptomes unveils
            diurnal molecular networks and deciphers perturbation gene
            signatures
  JOURNAL   Neuron 110 (20), 3318-3338 (2022)
   PUBMED   36265442
REFERENCE   4  (bases 1 to 3580)
  AUTHORS   Park,J., Lee,S.Y., Jeon,Y., Kim,K.M., Lee,J.K., Ko,J., Park,E.J.,
            Yoon,J.S., Kang,B.E., Ryu,D., Lee,H., Shin,S.J., Go,H. and Lee,C.W.
  TITLE     The Pellino1-PKCtheta; Signaling Axis Is an Essential Target for
            Improving Antitumor CD8+ T-lymphocyte Function
  JOURNAL   Cancer Immunol Res 10 (3), 327-342 (2022)
   PUBMED   35058288
REFERENCE   5  (bases 1 to 3580)
  AUTHORS   He,Y., Yang,Z., Zhao,C.S., Xiao,Z., Gong,Y., Li,Y.Y., Chen,Y.,
            Du,Y., Feng,D., Altman,A. and Li,Y.
  TITLE     T-cell receptor (TCR) signaling promotes the assembly of
            RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via
            PKC-theta;-mediated phosphorylation of RanGAP1
  JOURNAL   Elife 10, e67123 (2021)
   PUBMED   34110283
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3580)
  AUTHORS   Zappelli,F., Willems,D., Osada,S., Ohno,S., Wetsel,W.C.,
            Molinaro,M., Cossu,G. and Bouche,M.
  TITLE     The inhibition of differentiation caused by TGFbeta in fetal
            myoblasts is dependent upon selective expression of PKCtheta: a
            possible molecular basis for myoblast diversification during limb
            histogenesis
  JOURNAL   Dev Biol 180 (1), 156-164 (1996)
   PUBMED   8948582
REFERENCE   7  (bases 1 to 3580)
  AUTHORS   Bareggi,R., Grill,V., Zweyer,M., Narducci,P. and Martelli,A.M.
  TITLE     Distribution of the extended family of protein kinase C isoenzymes
            in fetal organs of mice: an immunohistochemical study
  JOURNAL   Cell Tissue Res 280 (3), 617-625 (1995)
   PUBMED   7606770
REFERENCE   8  (bases 1 to 3580)
  AUTHORS   Hilgenberg,L. and Miles,K.
  TITLE     Developmental regulation of a protein kinase C isoform localized in
            the neuromuscular junction
  JOURNAL   J Cell Sci 108 (Pt 1), 51-61 (1995)
   PUBMED   7738116
REFERENCE   9  (bases 1 to 3580)
  AUTHORS   Mischak,H., Goodnight,J., Henderson,D.W., Osada,S., Ohno,S. and
            Mushinski,J.F.
  TITLE     Unique expression pattern of protein kinase C-theta: high mRNA
            levels in normal mouse testes and in T-lymphocytic cells and
            neoplasms
  JOURNAL   FEBS Lett 326 (1-3), 51-55 (1993)
   PUBMED   8325388
REFERENCE   10 (bases 1 to 3580)
  AUTHORS   Osada,S., Mizuno,K., Saido,T.C., Suzuki,K., Kuroki,T. and Ohno,S.
  TITLE     A new member of the protein kinase C family, nPKC theta,
            predominantly expressed in skeletal muscle
  JOURNAL   Mol Cell Biol 12 (9), 3930-3938 (1992)
   PUBMED   1508194
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL928715.11 and AL929020.10.
            
            On Mar 18, 2023 this sequence version replaced NM_008859.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D11091.1, SRR13861890.193763.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-350               AL928715.11        88008-88357
            351-477             AL929020.10        7894-8020
            478-677             AL929020.10        13565-13764
            678-738             AL929020.10        17053-17113
            739-901             AL929020.10        26288-26450
            902-933             AL929020.10        27834-27865
            934-1019            AL929020.10        27963-28048
            1020-1149           AL929020.10        29236-29365
            1150-1259           AL929020.10        34104-34213
            1260-1377           AL929020.10        35206-35323
            1378-1538           AL929020.10        37192-37352
            1539-1712           AL929020.10        41688-41861
            1713-1804           AL929020.10        58050-58141
            1805-1867           AL929020.10        60501-60563
            1868-2006           AL929020.10        64781-64919
            2007-2195           AL929020.10        71424-71612
            2196-2324           AL929020.10        80330-80458
            2325-3580           AL929020.10        81002-82257
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 8.42 cM"
     gene            1..3580
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /note="protein kinase C, theta"
                     /db_xref="GeneID:18761"
                     /db_xref="MGI:MGI:97601"
     exon            1..350
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            351..477
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     CDS             360..2483
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /EC_number="2.7.11.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein kinase C theta type; nPKC-theta; PKC theta"
                     /codon_start=1
                     /product="protein kinase C theta type isoform 1"
                     /protein_id="NP_032885.1"
                     /db_xref="CCDS:CCDS15682.1"
                     /db_xref="GeneID:18761"
                     /db_xref="MGI:MGI:97601"
                     /translation="MSPFLRIGLSNFDCGTCQACQGEAVNPYCAVLVKEYVESENGQM
                     YIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGR
                     TEIWLELKPQGRMLMNARYFLEMSDTKDMSEFENEGFFALHQRRGAIKQAKVHHVKCH
                     EFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRE
                     TMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTK
                     VANLCGINQKLMAEALAMIESTQQARSLRDSEHIFREGPVEIGLPCSTKNETRPPCVP
                     TPGKREPQGISWDSPLDGSNKSAGPPEPEVSMRRTSLQLKLKIDDFILHKMLGKGSFG
                     KVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQT
                     KENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKGIVYRDLKLD
                     NILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFG
                     VLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGV
                     RGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRAL
                     INSMDQNMFSNFSFINPGMETLICS"
     exon            478..677
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            678..738
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            739..901
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            902..933
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1019
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1149
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1259
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1377
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1538
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1712
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1804
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1867
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..2006
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2195
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..2324
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..3580
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3033..3038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /note="hexamer: AATAAA"
     polyA_site      3057
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /note="major polyA site"
     regulatory      3561..3566
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /note="hexamer: AATAAA"
     polyA_site      3574
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
     polyA_site      3580
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
ORIGIN      
        1 ctggcactca ggcggctgag gctctggcgt ccgtcccgct tcgcgctgcc tgcggcgtcg
       61 ctccccgccc ggtgactgct tcatccagac ctcctgcagc ctccgggtcc cagtcgcggc
      121 agagcgacaa gtggccagtg aggcgtgagg atggagggtg gggacagagc cagcaggagg
      181 aaggccgaga gcgctcgcgg aacgcaacca ggcgcgcgtc cctgagccgg cgtggcgagc
      241 gccctctggc cggtgccgcc accggagtac ccttgggtcg ccaggcccgc gccagtcccc
      301 gccatccgag caacagcggc gctgctctgg gaccgcggcc gcgacaccag ggaacaacca
      361 tgtcaccgtt tcttcgaatc ggtttatcca actttgactg tgggacctgc caagcttgtc
      421 agggagaggc agtgaacccc tactgcgctg tgcttgtcaa agagtatgtg gaatcagaaa
      481 atgggcagat gtacatccag aaaaagccaa ccatgtaccc accttgggac agcacctttg
      541 acgcccacat taacaaggga agggtgatgc agatcatcgt gaagggcaag aatgtagacc
      601 tcatctcaga aacaaccgtg gaactctact ccctggcgga gagatgccgc aagaacaatg
      661 ggcggacaga aatatggtta gagctgaaac ctcaaggccg aatgctaatg aatgcaagat
      721 actttctgga aatgagtgac acaaaggaca tgagtgagtt tgagaatgaa ggattctttg
      781 cactgcatca gcgccgagga gccatcaaac aggccaaagt ccaccatgtc aagtgtcacg
      841 agttcacggc cacctttttc cctcaaccca cattttgctc tgtctgccat gaatttgtct
      901 ggggcctgaa caagcagggt taccagtgcc gacagtgtaa tgcagcgatt cacaagaagt
      961 gcattgataa agtgatagcc aagtgcacag gatccgcaat caatagccga gaaaccatgt
     1021 tccataagga gagattcaag atcgacatgc cacacagatt caaagtctac aactacaaga
     1081 gtccaacctt ctgtgagcac tgtggtaccc tgctctgggg gctggcgagg caaggactca
     1141 aatgtgatgc atgtggcatg aacgtccacc accgatgcca gacaaaggtt gccaatcttt
     1201 gtggtataaa ccagaagcta atggctgaag cactagcgat gattgaaagc acccaacagg
     1261 ctcgctcctt acgagattca gaacacatct tccgagaagg cccagttgaa attggtctcc
     1321 catgctccac caaaaacgaa accaggccac catgcgtacc aacacctggg aaaagagaac
     1381 cccagggcat ttcctgggat tcccctttgg atgggtcaaa taaatcggcc ggtcctcctg
     1441 aacccgaagt gagcatgcgc aggacttcac tgcagctgaa actgaagatc gatgacttca
     1501 tcctgcacaa gatgttggga aaaggaagtt ttggcaaggt cttcctggca gagttcaaga
     1561 gaaccaatca gtttttcgca ataaaagcct taaagaaaga tgtggtgttg atggatgatg
     1621 acgtcgagtg tacaatggtg gaaaagaggg ttctgtcctt ggcatgggag catccatttc
     1681 taacacacat gttctgcaca ttccagacca aggaaaatct ctttttcgtg atggagtatc
     1741 tcaatggagg cgacttaatg taccacatcc aaagttgcca caaatttgat ctttccagag
     1801 ccacgtttta tgctgctgag gtcatccttg gtctgcagtt ccttcattcc aaaggaattg
     1861 tctacaggga cctgaagctt gataatatcc tgttagacag agatggacat atcaaaatag
     1921 cagactttgg gatgtgcaaa gagaacatgc taggagatgc gaagacaaat actttctgtg
     1981 gaactcctga ctacattgct ccggagatct tgctgggtca gaagtacaac cattccgtcg
     2041 actggtggtc cttcggggtg ctcgtttatg agatgctgat tggccagtcc cccttccacg
     2101 ggcaggacga ggaggagctg ttccactcca tccgcatgga caaccccttc tacccgaggt
     2161 ggctcgaaag ggaggccaag gaccttctag tgaagctttt tgtgagagaa cctgagaaga
     2221 ggctgggagt gagaggagac atccgccagc atcctttgtt tcgagagatc aactgggaag
     2281 agcttgaaag gaaagagatt gacccaccct tcagaccaaa agtgaaatca ccatatgact
     2341 gtagcaattt cgacaaggaa ttcctaagtg agaaaccccg gctatcattc gccgacagag
     2401 cactcatcaa cagcatggac cagaacatgt tcagcaactt ttccttcatt aacccaggga
     2461 tggagactct catttgctcc tgaacctcat ctgtctccag actggaaggg atttgccttc
     2521 tctctgggaa ctggttcaag taacacttct gggggtgggg gtctcttttt cacgttagag
     2581 aagaaaagaa acactgcaaa ggcagggagg actgctgagc tccttgtgtg acttgttacc
     2641 tacagcacaa accacgccta cttcactaat gacatcatcc ctaatgacat catcccgtta
     2701 tatctcctgg aatctctcac agcagccctt gaagttagat cattattaac tctagtcatt
     2761 tacttgaaag atggttcccg atgctgtgaa agattcgaaa tgcagttctg ctcttgccct
     2821 agacaacagc tgctggttgg tgatgaacca aggcgcaagt ggaacagatt tctcaagact
     2881 ggagcagtga tcgcctgtta tagaagtcaa ttccactcaa ccacagagaa ggaaccacta
     2941 agccacgttg atgtgtgcat gtctgtggaa atgtcgatga cagaagggag ggaaagggga
     3001 agctctgagc agattgtaat gggaagctct ccaataaaca tagcatgaaa cttgaaattt
     3061 acaaatctgt tcattctggc tagccccaaa attcccaagg cagaggaaag taaagggcag
     3121 tgagcttagc agagcccttt gtcgccaaca gggaagggta aggatgtcgc ctacgtggaa
     3181 catcttatac acacagaagg aaagtataac caacaagggc agggtggttt acagctgcca
     3241 atcaaacctg ccctcccccc tctgttctca gttgatctct ctgtcagcgt aggtaggcac
     3301 tcattaccat cctcccatca tacaagaaat aaaatgcatg actcttctaa gataaagaaa
     3361 accaatccct tatcacgttg ttcccagtga tttgatggca aataagtccc tccttaggca
     3421 tcctgcaaga caacccaacc catgcatgct atttgcagta gtcagtcctg ttgagttaga
     3481 gtcctaacta tacacaatat cgtgcgatgt ttatatatgt tgatgagatg ttgtgatgat
     3541 aacgtggata tgtaaaaggg aataaaagaa gaaagaaaga
//
